Experiment: Trisodium citrate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4573 and Pf6N2E2_4574 are separated by 84 nucleotides Pf6N2E2_4574 and Pf6N2E2_4575 are separated by 27 nucleotides Pf6N2E2_4575 and Pf6N2E2_4576 are separated by 160 nucleotides Pf6N2E2_4576 and Pf6N2E2_4577 are separated by 34 nucleotides
Pf6N2E2_4573 - FIG022869: Oxidoreductase, GMC family, at 5,302,947 to 5,304,542
_4573
Pf6N2E2_4574 - FIG028932: hypothetical protein, at 5,304,627 to 5,305,172
_4574
Pf6N2E2_4575 - Coniferyl aldehyde dehydrogenase (EC 1.2.1.68), at 5,305,200 to 5,306,630
_4575
Pf6N2E2_4576 - Transcriptional regulator, TetR family, at 5,306,791 to 5,307,450
_4576
Pf6N2E2_4577 - Thiosulfate sulfurtransferase, rhodanese (EC 2.8.1.1), at 5,307,485 to 5,308,339
_4577
Position (kb)
5305
5306
5307 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 5304.299 kb on - strand, within Pf6N2E2_4573 at 5304.310 kb on + strand, within Pf6N2E2_4573 at 5304.311 kb on - strand, within Pf6N2E2_4573 at 5304.311 kb on - strand, within Pf6N2E2_4573 at 5304.490 kb on + strand at 5304.490 kb on + strand at 5304.490 kb on + strand at 5304.490 kb on + strand at 5304.491 kb on - strand at 5304.491 kb on - strand at 5304.491 kb on - strand at 5304.491 kb on - strand at 5304.491 kb on - strand at 5304.491 kb on - strand at 5304.491 kb on - strand at 5304.491 kb on - strand at 5304.557 kb on + strand at 5304.557 kb on + strand at 5304.557 kb on + strand at 5304.557 kb on + strand at 5304.557 kb on + strand at 5304.557 kb on + strand at 5304.557 kb on + strand at 5304.557 kb on + strand at 5304.557 kb on + strand at 5304.558 kb on - strand at 5304.558 kb on - strand at 5304.558 kb on - strand at 5304.558 kb on - strand at 5304.558 kb on - strand at 5304.560 kb on + strand at 5304.560 kb on + strand at 5304.560 kb on + strand at 5304.560 kb on + strand at 5304.560 kb on + strand at 5304.561 kb on - strand at 5304.561 kb on - strand at 5304.561 kb on - strand at 5304.561 kb on - strand at 5304.563 kb on + strand at 5304.683 kb on - strand, within Pf6N2E2_4574 at 5304.716 kb on + strand, within Pf6N2E2_4574 at 5304.716 kb on + strand, within Pf6N2E2_4574 at 5304.716 kb on + strand, within Pf6N2E2_4574 at 5304.716 kb on + strand, within Pf6N2E2_4574 at 5304.717 kb on - strand, within Pf6N2E2_4574 at 5304.717 kb on - strand, within Pf6N2E2_4574 at 5304.717 kb on - strand, within Pf6N2E2_4574 at 5304.717 kb on - strand, within Pf6N2E2_4574 at 5304.717 kb on - strand, within Pf6N2E2_4574 at 5305.152 kb on - strand at 5305.174 kb on - strand at 5305.179 kb on + strand at 5305.179 kb on + strand at 5305.179 kb on + strand at 5305.179 kb on + strand at 5305.255 kb on + strand at 5305.480 kb on + strand, within Pf6N2E2_4575 at 5305.481 kb on - strand, within Pf6N2E2_4575 at 5305.484 kb on - strand, within Pf6N2E2_4575 at 5305.516 kb on + strand, within Pf6N2E2_4575 at 5305.516 kb on + strand, within Pf6N2E2_4575 at 5305.516 kb on + strand, within Pf6N2E2_4575 at 5305.516 kb on + strand, within Pf6N2E2_4575 at 5305.516 kb on + strand, within Pf6N2E2_4575 at 5305.516 kb on + strand, within Pf6N2E2_4575 at 5305.517 kb on - strand, within Pf6N2E2_4575 at 5305.517 kb on - strand, within Pf6N2E2_4575 at 5305.517 kb on - strand, within Pf6N2E2_4575 at 5305.517 kb on - strand, within Pf6N2E2_4575 at 5305.517 kb on - strand, within Pf6N2E2_4575 at 5305.517 kb on - strand, within Pf6N2E2_4575 at 5305.517 kb on - strand, within Pf6N2E2_4575 at 5305.517 kb on - strand, within Pf6N2E2_4575 at 5305.544 kb on - strand, within Pf6N2E2_4575 at 5305.744 kb on + strand, within Pf6N2E2_4575 at 5305.744 kb on + strand, within Pf6N2E2_4575 at 5305.744 kb on + strand, within Pf6N2E2_4575 at 5305.744 kb on + strand, within Pf6N2E2_4575 at 5305.745 kb on - strand, within Pf6N2E2_4575 at 5305.840 kb on + strand, within Pf6N2E2_4575 at 5305.840 kb on + strand, within Pf6N2E2_4575 at 5305.841 kb on - strand, within Pf6N2E2_4575 at 5305.841 kb on - strand, within Pf6N2E2_4575 at 5306.209 kb on - strand, within Pf6N2E2_4575 at 5306.239 kb on + strand, within Pf6N2E2_4575 at 5306.239 kb on + strand, within Pf6N2E2_4575 at 5306.239 kb on + strand, within Pf6N2E2_4575 at 5306.240 kb on - strand, within Pf6N2E2_4575 at 5306.240 kb on - strand, within Pf6N2E2_4575 at 5306.240 kb on - strand, within Pf6N2E2_4575 at 5306.281 kb on + strand, within Pf6N2E2_4575 at 5306.281 kb on + strand, within Pf6N2E2_4575 at 5306.281 kb on + strand, within Pf6N2E2_4575 at 5306.282 kb on - strand, within Pf6N2E2_4575 at 5306.369 kb on - strand, within Pf6N2E2_4575 at 5306.481 kb on + strand, within Pf6N2E2_4575 at 5306.482 kb on - strand, within Pf6N2E2_4575 at 5306.482 kb on - strand, within Pf6N2E2_4575 at 5306.546 kb on - strand at 5306.611 kb on + strand at 5306.611 kb on + strand at 5306.611 kb on + strand at 5306.645 kb on + strand at 5306.646 kb on - strand at 5306.648 kb on + strand at 5306.648 kb on + strand at 5306.648 kb on + strand at 5306.648 kb on + strand at 5306.648 kb on + strand at 5306.648 kb on + strand at 5306.648 kb on + strand at 5306.648 kb on + strand at 5306.649 kb on - strand at 5306.649 kb on - strand at 5306.649 kb on - strand at 5306.649 kb on - strand at 5306.651 kb on + strand at 5306.651 kb on + strand at 5306.651 kb on + strand at 5306.651 kb on + strand at 5306.652 kb on - strand at 5306.652 kb on - strand at 5306.652 kb on - strand at 5306.652 kb on - strand at 5306.652 kb on - strand at 5306.674 kb on + strand at 5306.675 kb on - strand at 5306.675 kb on - strand at 5306.675 kb on - strand at 5306.675 kb on - strand at 5306.675 kb on - strand at 5306.676 kb on + strand at 5306.677 kb on - strand at 5306.737 kb on + strand at 5306.737 kb on + strand at 5306.737 kb on + strand at 5306.738 kb on - strand at 5306.738 kb on - strand at 5306.738 kb on - strand at 5306.766 kb on + strand at 5306.767 kb on - strand at 5306.908 kb on + strand, within Pf6N2E2_4576 at 5306.909 kb on - strand, within Pf6N2E2_4576 at 5306.909 kb on - strand, within Pf6N2E2_4576 at 5306.912 kb on - strand, within Pf6N2E2_4576 at 5306.960 kb on - strand, within Pf6N2E2_4576 at 5306.960 kb on - strand, within Pf6N2E2_4576 at 5306.960 kb on - strand, within Pf6N2E2_4576 at 5307.031 kb on + strand, within Pf6N2E2_4576 at 5307.031 kb on + strand, within Pf6N2E2_4576 at 5307.032 kb on - strand, within Pf6N2E2_4576 at 5307.032 kb on - strand, within Pf6N2E2_4576 at 5307.168 kb on + strand, within Pf6N2E2_4576 at 5307.169 kb on - strand, within Pf6N2E2_4576 at 5307.169 kb on - strand, within Pf6N2E2_4576 at 5307.172 kb on + strand, within Pf6N2E2_4576 at 5307.172 kb on + strand, within Pf6N2E2_4576 at 5307.172 kb on + strand, within Pf6N2E2_4576 at 5307.172 kb on + strand, within Pf6N2E2_4576 at 5307.172 kb on + strand, within Pf6N2E2_4576 at 5307.172 kb on + strand, within Pf6N2E2_4576 at 5307.173 kb on - strand, within Pf6N2E2_4576 at 5307.173 kb on - strand, within Pf6N2E2_4576 at 5307.173 kb on - strand, within Pf6N2E2_4576 at 5307.173 kb on - strand, within Pf6N2E2_4576 at 5307.173 kb on - strand, within Pf6N2E2_4576 at 5307.173 kb on - strand, within Pf6N2E2_4576 at 5307.334 kb on + strand, within Pf6N2E2_4576 at 5307.341 kb on + strand, within Pf6N2E2_4576 at 5307.341 kb on + strand, within Pf6N2E2_4576 at 5307.341 kb on + strand, within Pf6N2E2_4576 at 5307.341 kb on + strand, within Pf6N2E2_4576 at 5307.342 kb on - strand, within Pf6N2E2_4576 at 5307.342 kb on - strand, within Pf6N2E2_4576 at 5307.409 kb on + strand at 5307.409 kb on + strand at 5307.410 kb on - strand at 5307.410 kb on - strand at 5307.412 kb on + strand at 5307.412 kb on + strand at 5307.412 kb on + strand at 5307.412 kb on + strand at 5307.412 kb on + strand at 5307.412 kb on + strand at 5307.412 kb on + strand at 5307.412 kb on + strand at 5307.412 kb on + strand at 5307.412 kb on + strand at 5307.412 kb on + strand at 5307.412 kb on + strand at 5307.413 kb on - strand at 5307.413 kb on - strand at 5307.413 kb on - strand at 5307.413 kb on - strand at 5307.413 kb on - strand at 5307.413 kb on - strand at 5307.413 kb on - strand at 5307.413 kb on - strand at 5307.413 kb on - strand at 5307.413 kb on - strand at 5307.413 kb on - strand at 5307.413 kb on - strand at 5307.448 kb on + strand at 5307.449 kb on - strand at 5307.449 kb on - strand at 5307.587 kb on + strand, within Pf6N2E2_4577 at 5307.588 kb on - strand, within Pf6N2E2_4577 at 5307.603 kb on - strand, within Pf6N2E2_4577 at 5307.603 kb on - strand, within Pf6N2E2_4577
Per-strain Table
Position Strand Gene LocusTag Fraction Trisodium citrate (C) remove 5,304,299 - Pf6N2E2_4573 0.85 +0.1 5,304,310 + Pf6N2E2_4573 0.85 +0.4 5,304,311 - Pf6N2E2_4573 0.85 -0.4 5,304,311 - Pf6N2E2_4573 0.85 -0.0 5,304,490 + -0.3 5,304,490 + +0.7 5,304,490 + +0.8 5,304,490 + +1.3 5,304,491 - -1.3 5,304,491 - -1.0 5,304,491 - +0.3 5,304,491 - +0.1 5,304,491 - -0.1 5,304,491 - +0.9 5,304,491 - -1.3 5,304,491 - +1.2 5,304,557 + -0.4 5,304,557 + +1.1 5,304,557 + +0.4 5,304,557 + +0.0 5,304,557 + -1.0 5,304,557 + -0.2 5,304,557 + -0.8 5,304,557 + -0.3 5,304,557 + +0.6 5,304,558 - -0.1 5,304,558 - -1.4 5,304,558 - -0.5 5,304,558 - -0.1 5,304,558 - +0.5 5,304,560 + +0.4 5,304,560 + -0.1 5,304,560 + -0.2 5,304,560 + -1.2 5,304,560 + +0.4 5,304,561 - -1.2 5,304,561 - -0.1 5,304,561 - -0.3 5,304,561 - +0.5 5,304,563 + +0.9 5,304,683 - Pf6N2E2_4574 0.10 +0.6 5,304,716 + Pf6N2E2_4574 0.16 -0.7 5,304,716 + Pf6N2E2_4574 0.16 -0.0 5,304,716 + Pf6N2E2_4574 0.16 +0.3 5,304,716 + Pf6N2E2_4574 0.16 -0.0 5,304,717 - Pf6N2E2_4574 0.16 +0.1 5,304,717 - Pf6N2E2_4574 0.16 +1.3 5,304,717 - Pf6N2E2_4574 0.16 +0.0 5,304,717 - Pf6N2E2_4574 0.16 -0.5 5,304,717 - Pf6N2E2_4574 0.16 +0.4 5,305,152 - +1.5 5,305,174 - +1.0 5,305,179 + -0.2 5,305,179 + -0.2 5,305,179 + +0.3 5,305,179 + +0.4 5,305,255 + +0.3 5,305,480 + Pf6N2E2_4575 0.20 +0.2 5,305,481 - Pf6N2E2_4575 0.20 +0.6 5,305,484 - Pf6N2E2_4575 0.20 +0.8 5,305,516 + Pf6N2E2_4575 0.22 -0.0 5,305,516 + Pf6N2E2_4575 0.22 -0.6 5,305,516 + Pf6N2E2_4575 0.22 +1.6 5,305,516 + Pf6N2E2_4575 0.22 +0.6 5,305,516 + Pf6N2E2_4575 0.22 +0.2 5,305,516 + Pf6N2E2_4575 0.22 +0.1 5,305,517 - Pf6N2E2_4575 0.22 +1.2 5,305,517 - Pf6N2E2_4575 0.22 -0.3 5,305,517 - Pf6N2E2_4575 0.22 -1.2 5,305,517 - Pf6N2E2_4575 0.22 +0.3 5,305,517 - Pf6N2E2_4575 0.22 +0.4 5,305,517 - Pf6N2E2_4575 0.22 -0.2 5,305,517 - Pf6N2E2_4575 0.22 +0.8 5,305,517 - Pf6N2E2_4575 0.22 +1.2 5,305,544 - Pf6N2E2_4575 0.24 -0.1 5,305,744 + Pf6N2E2_4575 0.38 +0.2 5,305,744 + Pf6N2E2_4575 0.38 -0.9 5,305,744 + Pf6N2E2_4575 0.38 -0.3 5,305,744 + Pf6N2E2_4575 0.38 -0.7 5,305,745 - Pf6N2E2_4575 0.38 +1.7 5,305,840 + Pf6N2E2_4575 0.45 -0.4 5,305,840 + Pf6N2E2_4575 0.45 +0.0 5,305,841 - Pf6N2E2_4575 0.45 +1.9 5,305,841 - Pf6N2E2_4575 0.45 +0.7 5,306,209 - Pf6N2E2_4575 0.71 +0.7 5,306,239 + Pf6N2E2_4575 0.73 -0.1 5,306,239 + Pf6N2E2_4575 0.73 -0.2 5,306,239 + Pf6N2E2_4575 0.73 -0.4 5,306,240 - Pf6N2E2_4575 0.73 +0.7 5,306,240 - Pf6N2E2_4575 0.73 +1.3 5,306,240 - Pf6N2E2_4575 0.73 +1.0 5,306,281 + Pf6N2E2_4575 0.76 +1.1 5,306,281 + Pf6N2E2_4575 0.76 -0.3 5,306,281 + Pf6N2E2_4575 0.76 -0.8 5,306,282 - Pf6N2E2_4575 0.76 +0.6 5,306,369 - Pf6N2E2_4575 0.82 +0.1 5,306,481 + Pf6N2E2_4575 0.90 -2.3 5,306,482 - Pf6N2E2_4575 0.90 +2.6 5,306,482 - Pf6N2E2_4575 0.90 +1.1 5,306,546 - -0.8 5,306,611 + +0.6 5,306,611 + -0.7 5,306,611 + +0.9 5,306,645 + +0.1 5,306,646 - +0.5 5,306,648 + -0.2 5,306,648 + -0.1 5,306,648 + -1.0 5,306,648 + +0.3 5,306,648 + -0.9 5,306,648 + +0.5 5,306,648 + +0.2 5,306,648 + +1.9 5,306,649 - +0.1 5,306,649 - +1.2 5,306,649 - +0.8 5,306,649 - +0.1 5,306,651 + +0.6 5,306,651 + +1.0 5,306,651 + +0.3 5,306,651 + +0.1 5,306,652 - +0.9 5,306,652 - +0.9 5,306,652 - +0.3 5,306,652 - +1.0 5,306,652 - -0.5 5,306,674 + -1.1 5,306,675 - +0.6 5,306,675 - +0.5 5,306,675 - -1.1 5,306,675 - +1.9 5,306,675 - -1.3 5,306,676 + +0.8 5,306,677 - +1.4 5,306,737 + +0.9 5,306,737 + +0.0 5,306,737 + +0.5 5,306,738 - +0.6 5,306,738 - -0.7 5,306,738 - +0.4 5,306,766 + +0.2 5,306,767 - +1.0 5,306,908 + Pf6N2E2_4576 0.18 +2.6 5,306,909 - Pf6N2E2_4576 0.18 -1.1 5,306,909 - Pf6N2E2_4576 0.18 +0.3 5,306,912 - Pf6N2E2_4576 0.18 +1.1 5,306,960 - Pf6N2E2_4576 0.26 -0.4 5,306,960 - Pf6N2E2_4576 0.26 +0.5 5,306,960 - Pf6N2E2_4576 0.26 +0.5 5,307,031 + Pf6N2E2_4576 0.36 -0.3 5,307,031 + Pf6N2E2_4576 0.36 +0.6 5,307,032 - Pf6N2E2_4576 0.37 +0.1 5,307,032 - Pf6N2E2_4576 0.37 +0.6 5,307,168 + Pf6N2E2_4576 0.57 -0.5 5,307,169 - Pf6N2E2_4576 0.57 +1.8 5,307,169 - Pf6N2E2_4576 0.57 -1.0 5,307,172 + Pf6N2E2_4576 0.58 -0.2 5,307,172 + Pf6N2E2_4576 0.58 +0.2 5,307,172 + Pf6N2E2_4576 0.58 +0.1 5,307,172 + Pf6N2E2_4576 0.58 -0.5 5,307,172 + Pf6N2E2_4576 0.58 +1.5 5,307,172 + Pf6N2E2_4576 0.58 +1.9 5,307,173 - Pf6N2E2_4576 0.58 +1.5 5,307,173 - Pf6N2E2_4576 0.58 +0.3 5,307,173 - Pf6N2E2_4576 0.58 +0.6 5,307,173 - Pf6N2E2_4576 0.58 +0.4 5,307,173 - Pf6N2E2_4576 0.58 +0.3 5,307,173 - Pf6N2E2_4576 0.58 +1.9 5,307,334 + Pf6N2E2_4576 0.82 -0.4 5,307,341 + Pf6N2E2_4576 0.83 +0.8 5,307,341 + Pf6N2E2_4576 0.83 -0.7 5,307,341 + Pf6N2E2_4576 0.83 +0.6 5,307,341 + Pf6N2E2_4576 0.83 +0.3 5,307,342 - Pf6N2E2_4576 0.83 +0.5 5,307,342 - Pf6N2E2_4576 0.83 -0.4 5,307,409 + -0.3 5,307,409 + +0.8 5,307,410 - +1.1 5,307,410 - +1.5 5,307,412 + +0.2 5,307,412 + +1.0 5,307,412 + +0.3 5,307,412 + -1.7 5,307,412 + +0.2 5,307,412 + +0.1 5,307,412 + +0.6 5,307,412 + +0.3 5,307,412 + +0.9 5,307,412 + -1.0 5,307,412 + +0.9 5,307,412 + -0.3 5,307,413 - -0.4 5,307,413 - +1.1 5,307,413 - -0.1 5,307,413 - +0.3 5,307,413 - +0.3 5,307,413 - -0.2 5,307,413 - -1.4 5,307,413 - -0.2 5,307,413 - -0.6 5,307,413 - +0.9 5,307,413 - +0.6 5,307,413 - -0.5 5,307,448 + +0.1 5,307,449 - -0.1 5,307,449 - +0.9 5,307,587 + Pf6N2E2_4577 0.12 +0.2 5,307,588 - Pf6N2E2_4577 0.12 -0.6 5,307,603 - Pf6N2E2_4577 0.14 +1.5 5,307,603 - Pf6N2E2_4577 0.14 -0.6
Or see this region's nucleotide sequence