Experiment: Trisodium citrate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4445 and Pf6N2E2_4446 are separated by 303 nucleotides Pf6N2E2_4446 and Pf6N2E2_4447 are separated by 3 nucleotides Pf6N2E2_4447 and Pf6N2E2_4448 are separated by 106 nucleotides Pf6N2E2_4448 and Pf6N2E2_4449 are separated by 54 nucleotides
Pf6N2E2_4445 - TPR domain protein, at 5,175,964 to 5,177,364
_4445
Pf6N2E2_4446 - hypothetical protein, at 5,177,668 to 5,177,781
_4446
Pf6N2E2_4447 - Cytochrome c family protein, at 5,177,785 to 5,179,572
_4447
Pf6N2E2_4448 - probable P23 protein, at 5,179,679 to 5,180,134
_4448
Pf6N2E2_4449 - Sigma cross-reacting protein 27A, at 5,180,189 to 5,180,857
_4449
Position (kb)
5177
5178
5179
5180 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 5176.805 kb on + strand, within Pf6N2E2_4445 at 5176.806 kb on - strand, within Pf6N2E2_4445 at 5176.806 kb on - strand, within Pf6N2E2_4445 at 5177.013 kb on + strand, within Pf6N2E2_4445 at 5177.014 kb on - strand, within Pf6N2E2_4445 at 5177.048 kb on + strand, within Pf6N2E2_4445 at 5177.049 kb on - strand, within Pf6N2E2_4445 at 5177.049 kb on - strand, within Pf6N2E2_4445 at 5177.049 kb on - strand, within Pf6N2E2_4445 at 5177.171 kb on + strand, within Pf6N2E2_4445 at 5177.171 kb on + strand, within Pf6N2E2_4445 at 5177.172 kb on - strand, within Pf6N2E2_4445 at 5177.172 kb on - strand, within Pf6N2E2_4445 at 5177.174 kb on + strand, within Pf6N2E2_4445 at 5177.174 kb on + strand, within Pf6N2E2_4445 at 5177.174 kb on + strand, within Pf6N2E2_4445 at 5177.174 kb on + strand, within Pf6N2E2_4445 at 5177.174 kb on + strand, within Pf6N2E2_4445 at 5177.174 kb on + strand, within Pf6N2E2_4445 at 5177.175 kb on - strand, within Pf6N2E2_4445 at 5177.175 kb on - strand, within Pf6N2E2_4445 at 5177.175 kb on - strand, within Pf6N2E2_4445 at 5177.175 kb on - strand, within Pf6N2E2_4445 at 5177.175 kb on - strand, within Pf6N2E2_4445 at 5177.282 kb on + strand at 5177.282 kb on + strand at 5177.430 kb on + strand at 5177.431 kb on - strand at 5177.464 kb on + strand at 5177.465 kb on - strand at 5177.498 kb on + strand at 5177.498 kb on + strand at 5177.498 kb on + strand at 5177.499 kb on - strand at 5177.511 kb on + strand at 5177.511 kb on + strand at 5177.511 kb on + strand at 5177.511 kb on + strand at 5177.511 kb on + strand at 5177.512 kb on - strand at 5177.515 kb on + strand at 5177.516 kb on - strand at 5177.709 kb on - strand, within Pf6N2E2_4446 at 5177.783 kb on + strand at 5177.783 kb on + strand at 5177.783 kb on + strand at 5177.791 kb on + strand at 5177.791 kb on + strand at 5177.792 kb on - strand at 5178.034 kb on + strand, within Pf6N2E2_4447 at 5178.034 kb on + strand, within Pf6N2E2_4447 at 5178.034 kb on + strand, within Pf6N2E2_4447 at 5178.034 kb on + strand, within Pf6N2E2_4447 at 5178.034 kb on + strand, within Pf6N2E2_4447 at 5178.034 kb on + strand, within Pf6N2E2_4447 at 5178.034 kb on + strand, within Pf6N2E2_4447 at 5178.035 kb on - strand, within Pf6N2E2_4447 at 5178.035 kb on - strand, within Pf6N2E2_4447 at 5178.035 kb on - strand, within Pf6N2E2_4447 at 5178.035 kb on - strand, within Pf6N2E2_4447 at 5178.091 kb on + strand, within Pf6N2E2_4447 at 5178.091 kb on + strand, within Pf6N2E2_4447 at 5178.091 kb on + strand, within Pf6N2E2_4447 at 5178.214 kb on + strand, within Pf6N2E2_4447 at 5178.214 kb on + strand, within Pf6N2E2_4447 at 5178.215 kb on - strand, within Pf6N2E2_4447 at 5178.223 kb on + strand, within Pf6N2E2_4447 at 5178.223 kb on + strand, within Pf6N2E2_4447 at 5178.223 kb on + strand, within Pf6N2E2_4447 at 5178.223 kb on + strand, within Pf6N2E2_4447 at 5178.223 kb on + strand, within Pf6N2E2_4447 at 5178.224 kb on - strand, within Pf6N2E2_4447 at 5178.224 kb on - strand, within Pf6N2E2_4447 at 5178.224 kb on - strand, within Pf6N2E2_4447 at 5178.224 kb on - strand, within Pf6N2E2_4447 at 5178.224 kb on - strand, within Pf6N2E2_4447 at 5178.224 kb on - strand, within Pf6N2E2_4447 at 5178.258 kb on + strand, within Pf6N2E2_4447 at 5178.258 kb on + strand, within Pf6N2E2_4447 at 5178.258 kb on + strand, within Pf6N2E2_4447 at 5178.259 kb on - strand, within Pf6N2E2_4447 at 5178.313 kb on + strand, within Pf6N2E2_4447 at 5178.313 kb on + strand, within Pf6N2E2_4447 at 5178.313 kb on + strand, within Pf6N2E2_4447 at 5178.313 kb on + strand, within Pf6N2E2_4447 at 5178.313 kb on + strand, within Pf6N2E2_4447 at 5178.314 kb on - strand, within Pf6N2E2_4447 at 5178.314 kb on - strand, within Pf6N2E2_4447 at 5178.314 kb on - strand, within Pf6N2E2_4447 at 5178.314 kb on - strand, within Pf6N2E2_4447 at 5178.314 kb on - strand, within Pf6N2E2_4447 at 5178.314 kb on - strand, within Pf6N2E2_4447 at 5178.499 kb on + strand, within Pf6N2E2_4447 at 5178.536 kb on - strand, within Pf6N2E2_4447 at 5178.562 kb on + strand, within Pf6N2E2_4447 at 5178.562 kb on + strand, within Pf6N2E2_4447 at 5178.562 kb on + strand, within Pf6N2E2_4447 at 5178.563 kb on - strand, within Pf6N2E2_4447 at 5178.563 kb on - strand, within Pf6N2E2_4447 at 5178.563 kb on - strand, within Pf6N2E2_4447 at 5178.563 kb on - strand, within Pf6N2E2_4447 at 5178.563 kb on - strand, within Pf6N2E2_4447 at 5178.563 kb on - strand, within Pf6N2E2_4447 at 5178.583 kb on + strand, within Pf6N2E2_4447 at 5178.583 kb on + strand, within Pf6N2E2_4447 at 5178.583 kb on + strand, within Pf6N2E2_4447 at 5178.584 kb on - strand, within Pf6N2E2_4447 at 5178.670 kb on + strand, within Pf6N2E2_4447 at 5178.796 kb on + strand, within Pf6N2E2_4447 at 5178.796 kb on + strand, within Pf6N2E2_4447 at 5178.796 kb on + strand, within Pf6N2E2_4447 at 5178.796 kb on + strand, within Pf6N2E2_4447 at 5178.796 kb on + strand, within Pf6N2E2_4447 at 5178.796 kb on + strand, within Pf6N2E2_4447 at 5178.796 kb on + strand, within Pf6N2E2_4447 at 5178.796 kb on + strand, within Pf6N2E2_4447 at 5178.796 kb on + strand, within Pf6N2E2_4447 at 5178.796 kb on + strand, within Pf6N2E2_4447 at 5178.797 kb on - strand, within Pf6N2E2_4447 at 5178.797 kb on - strand, within Pf6N2E2_4447 at 5178.797 kb on - strand, within Pf6N2E2_4447 at 5178.797 kb on - strand, within Pf6N2E2_4447 at 5178.797 kb on - strand, within Pf6N2E2_4447 at 5178.798 kb on + strand, within Pf6N2E2_4447 at 5178.798 kb on + strand, within Pf6N2E2_4447 at 5178.798 kb on + strand, within Pf6N2E2_4447 at 5178.798 kb on + strand, within Pf6N2E2_4447 at 5178.798 kb on + strand, within Pf6N2E2_4447 at 5178.798 kb on + strand, within Pf6N2E2_4447 at 5178.798 kb on + strand, within Pf6N2E2_4447 at 5178.799 kb on - strand, within Pf6N2E2_4447 at 5178.799 kb on - strand, within Pf6N2E2_4447 at 5178.799 kb on - strand, within Pf6N2E2_4447 at 5178.799 kb on - strand, within Pf6N2E2_4447 at 5178.799 kb on - strand, within Pf6N2E2_4447 at 5178.967 kb on + strand, within Pf6N2E2_4447 at 5178.967 kb on + strand, within Pf6N2E2_4447 at 5178.967 kb on + strand, within Pf6N2E2_4447 at 5178.967 kb on + strand, within Pf6N2E2_4447 at 5178.967 kb on + strand, within Pf6N2E2_4447 at 5178.967 kb on + strand, within Pf6N2E2_4447 at 5178.968 kb on - strand, within Pf6N2E2_4447 at 5179.096 kb on + strand, within Pf6N2E2_4447 at 5179.096 kb on + strand, within Pf6N2E2_4447 at 5179.096 kb on + strand, within Pf6N2E2_4447 at 5179.373 kb on + strand, within Pf6N2E2_4447 at 5179.373 kb on + strand, within Pf6N2E2_4447 at 5179.374 kb on - strand, within Pf6N2E2_4447 at 5179.374 kb on - strand, within Pf6N2E2_4447 at 5179.525 kb on + strand at 5179.525 kb on + strand at 5179.526 kb on - strand at 5179.573 kb on + strand at 5179.574 kb on - strand at 5179.620 kb on + strand at 5179.620 kb on + strand at 5179.620 kb on + strand at 5179.620 kb on + strand at 5179.620 kb on + strand at 5179.620 kb on + strand at 5179.620 kb on + strand at 5179.620 kb on + strand at 5179.620 kb on + strand at 5179.621 kb on - strand at 5179.621 kb on - strand at 5179.621 kb on - strand at 5179.662 kb on - strand at 5179.827 kb on + strand, within Pf6N2E2_4448 at 5179.856 kb on + strand, within Pf6N2E2_4448 at 5179.857 kb on - strand, within Pf6N2E2_4448 at 5180.061 kb on - strand, within Pf6N2E2_4448 at 5180.176 kb on - strand at 5180.264 kb on - strand, within Pf6N2E2_4449 at 5180.264 kb on - strand, within Pf6N2E2_4449 at 5180.280 kb on + strand, within Pf6N2E2_4449 at 5180.280 kb on + strand, within Pf6N2E2_4449 at 5180.280 kb on + strand, within Pf6N2E2_4449 at 5180.280 kb on + strand, within Pf6N2E2_4449 at 5180.280 kb on + strand, within Pf6N2E2_4449 at 5180.280 kb on + strand, within Pf6N2E2_4449 at 5180.280 kb on + strand, within Pf6N2E2_4449 at 5180.281 kb on - strand, within Pf6N2E2_4449 at 5180.281 kb on - strand, within Pf6N2E2_4449 at 5180.281 kb on - strand, within Pf6N2E2_4449 at 5180.281 kb on - strand, within Pf6N2E2_4449 at 5180.421 kb on + strand, within Pf6N2E2_4449 at 5180.422 kb on - strand, within Pf6N2E2_4449
Per-strain Table
Position Strand Gene LocusTag Fraction Trisodium citrate (C) remove 5,176,805 + Pf6N2E2_4445 0.60 -0.4 5,176,806 - Pf6N2E2_4445 0.60 -0.5 5,176,806 - Pf6N2E2_4445 0.60 +2.4 5,177,013 + Pf6N2E2_4445 0.75 +0.6 5,177,014 - Pf6N2E2_4445 0.75 +1.0 5,177,048 + Pf6N2E2_4445 0.77 +0.8 5,177,049 - Pf6N2E2_4445 0.77 +1.0 5,177,049 - Pf6N2E2_4445 0.77 -0.2 5,177,049 - Pf6N2E2_4445 0.77 -0.5 5,177,171 + Pf6N2E2_4445 0.86 -0.4 5,177,171 + Pf6N2E2_4445 0.86 +1.8 5,177,172 - Pf6N2E2_4445 0.86 -1.1 5,177,172 - Pf6N2E2_4445 0.86 -0.8 5,177,174 + Pf6N2E2_4445 0.86 +0.7 5,177,174 + Pf6N2E2_4445 0.86 +1.0 5,177,174 + Pf6N2E2_4445 0.86 +0.5 5,177,174 + Pf6N2E2_4445 0.86 +1.6 5,177,174 + Pf6N2E2_4445 0.86 +1.1 5,177,174 + Pf6N2E2_4445 0.86 +1.0 5,177,175 - Pf6N2E2_4445 0.86 -0.5 5,177,175 - Pf6N2E2_4445 0.86 +0.2 5,177,175 - Pf6N2E2_4445 0.86 -1.0 5,177,175 - Pf6N2E2_4445 0.86 -1.1 5,177,175 - Pf6N2E2_4445 0.86 +1.0 5,177,282 + +1.4 5,177,282 + +1.6 5,177,430 + +2.8 5,177,431 - +0.6 5,177,464 + +2.5 5,177,465 - -1.0 5,177,498 + +2.3 5,177,498 + +1.3 5,177,498 + +1.4 5,177,499 - +0.0 5,177,511 + +0.5 5,177,511 + +0.3 5,177,511 + +1.5 5,177,511 + +0.3 5,177,511 + +1.6 5,177,512 - -1.7 5,177,515 + +1.0 5,177,516 - -1.6 5,177,709 - Pf6N2E2_4446 0.36 -2.5 5,177,783 + -2.8 5,177,783 + -3.3 5,177,783 + -1.2 5,177,791 + -1.0 5,177,791 + -2.4 5,177,792 - -1.2 5,178,034 + Pf6N2E2_4447 0.14 -2.2 5,178,034 + Pf6N2E2_4447 0.14 -1.6 5,178,034 + Pf6N2E2_4447 0.14 -1.3 5,178,034 + Pf6N2E2_4447 0.14 -3.1 5,178,034 + Pf6N2E2_4447 0.14 -2.8 5,178,034 + Pf6N2E2_4447 0.14 -1.4 5,178,034 + Pf6N2E2_4447 0.14 -2.5 5,178,035 - Pf6N2E2_4447 0.14 -2.9 5,178,035 - Pf6N2E2_4447 0.14 -1.7 5,178,035 - Pf6N2E2_4447 0.14 -2.2 5,178,035 - Pf6N2E2_4447 0.14 -2.3 5,178,091 + Pf6N2E2_4447 0.17 -3.5 5,178,091 + Pf6N2E2_4447 0.17 -2.2 5,178,091 + Pf6N2E2_4447 0.17 -2.9 5,178,214 + Pf6N2E2_4447 0.24 -2.5 5,178,214 + Pf6N2E2_4447 0.24 -1.9 5,178,215 - Pf6N2E2_4447 0.24 -2.7 5,178,223 + Pf6N2E2_4447 0.24 -1.8 5,178,223 + Pf6N2E2_4447 0.24 -0.9 5,178,223 + Pf6N2E2_4447 0.24 -3.8 5,178,223 + Pf6N2E2_4447 0.24 -0.5 5,178,223 + Pf6N2E2_4447 0.24 -2.3 5,178,224 - Pf6N2E2_4447 0.25 -1.9 5,178,224 - Pf6N2E2_4447 0.25 -3.1 5,178,224 - Pf6N2E2_4447 0.25 -3.2 5,178,224 - Pf6N2E2_4447 0.25 -1.7 5,178,224 - Pf6N2E2_4447 0.25 -0.2 5,178,224 - Pf6N2E2_4447 0.25 -2.7 5,178,258 + Pf6N2E2_4447 0.26 -3.1 5,178,258 + Pf6N2E2_4447 0.26 -2.3 5,178,258 + Pf6N2E2_4447 0.26 -3.9 5,178,259 - Pf6N2E2_4447 0.27 -3.5 5,178,313 + Pf6N2E2_4447 0.30 -1.5 5,178,313 + Pf6N2E2_4447 0.30 -0.9 5,178,313 + Pf6N2E2_4447 0.30 -3.1 5,178,313 + Pf6N2E2_4447 0.30 -1.4 5,178,313 + Pf6N2E2_4447 0.30 -1.1 5,178,314 - Pf6N2E2_4447 0.30 -3.1 5,178,314 - Pf6N2E2_4447 0.30 -2.0 5,178,314 - Pf6N2E2_4447 0.30 -2.1 5,178,314 - Pf6N2E2_4447 0.30 -2.8 5,178,314 - Pf6N2E2_4447 0.30 -1.5 5,178,314 - Pf6N2E2_4447 0.30 +1.0 5,178,499 + Pf6N2E2_4447 0.40 -1.5 5,178,536 - Pf6N2E2_4447 0.42 -3.2 5,178,562 + Pf6N2E2_4447 0.43 -3.5 5,178,562 + Pf6N2E2_4447 0.43 -3.6 5,178,562 + Pf6N2E2_4447 0.43 -2.9 5,178,563 - Pf6N2E2_4447 0.44 -4.0 5,178,563 - Pf6N2E2_4447 0.44 -1.2 5,178,563 - Pf6N2E2_4447 0.44 -2.9 5,178,563 - Pf6N2E2_4447 0.44 -3.7 5,178,563 - Pf6N2E2_4447 0.44 -0.2 5,178,563 - Pf6N2E2_4447 0.44 -2.6 5,178,583 + Pf6N2E2_4447 0.45 -1.5 5,178,583 + Pf6N2E2_4447 0.45 -3.4 5,178,583 + Pf6N2E2_4447 0.45 -2.0 5,178,584 - Pf6N2E2_4447 0.45 -3.1 5,178,670 + Pf6N2E2_4447 0.49 -1.2 5,178,796 + Pf6N2E2_4447 0.57 -4.7 5,178,796 + Pf6N2E2_4447 0.57 -1.4 5,178,796 + Pf6N2E2_4447 0.57 -2.2 5,178,796 + Pf6N2E2_4447 0.57 -3.4 5,178,796 + Pf6N2E2_4447 0.57 -1.4 5,178,796 + Pf6N2E2_4447 0.57 -1.3 5,178,796 + Pf6N2E2_4447 0.57 -3.5 5,178,796 + Pf6N2E2_4447 0.57 -3.9 5,178,796 + Pf6N2E2_4447 0.57 -2.2 5,178,796 + Pf6N2E2_4447 0.57 -2.8 5,178,797 - Pf6N2E2_4447 0.57 -2.4 5,178,797 - Pf6N2E2_4447 0.57 -2.9 5,178,797 - Pf6N2E2_4447 0.57 -2.7 5,178,797 - Pf6N2E2_4447 0.57 -1.6 5,178,797 - Pf6N2E2_4447 0.57 -1.5 5,178,798 + Pf6N2E2_4447 0.57 -3.1 5,178,798 + Pf6N2E2_4447 0.57 -3.1 5,178,798 + Pf6N2E2_4447 0.57 -1.2 5,178,798 + Pf6N2E2_4447 0.57 -0.6 5,178,798 + Pf6N2E2_4447 0.57 -3.6 5,178,798 + Pf6N2E2_4447 0.57 -3.8 5,178,798 + Pf6N2E2_4447 0.57 -1.9 5,178,799 - Pf6N2E2_4447 0.57 -3.2 5,178,799 - Pf6N2E2_4447 0.57 -1.9 5,178,799 - Pf6N2E2_4447 0.57 -4.2 5,178,799 - Pf6N2E2_4447 0.57 -3.3 5,178,799 - Pf6N2E2_4447 0.57 -3.2 5,178,967 + Pf6N2E2_4447 0.66 -3.3 5,178,967 + Pf6N2E2_4447 0.66 -2.9 5,178,967 + Pf6N2E2_4447 0.66 -1.3 5,178,967 + Pf6N2E2_4447 0.66 -3.6 5,178,967 + Pf6N2E2_4447 0.66 -2.7 5,178,967 + Pf6N2E2_4447 0.66 -2.8 5,178,968 - Pf6N2E2_4447 0.66 -2.1 5,179,096 + Pf6N2E2_4447 0.73 -3.6 5,179,096 + Pf6N2E2_4447 0.73 -2.5 5,179,096 + Pf6N2E2_4447 0.73 -1.6 5,179,373 + Pf6N2E2_4447 0.89 +1.0 5,179,373 + Pf6N2E2_4447 0.89 -2.1 5,179,374 - Pf6N2E2_4447 0.89 -1.7 5,179,374 - Pf6N2E2_4447 0.89 -2.9 5,179,525 + -1.3 5,179,525 + -1.4 5,179,526 - -1.9 5,179,573 + -0.5 5,179,574 - -0.7 5,179,620 + -0.5 5,179,620 + -0.4 5,179,620 + -0.7 5,179,620 + -0.8 5,179,620 + -0.0 5,179,620 + -1.1 5,179,620 + -0.4 5,179,620 + +1.0 5,179,620 + -3.6 5,179,621 - -0.7 5,179,621 - -0.4 5,179,621 - -2.3 5,179,662 - +0.0 5,179,827 + Pf6N2E2_4448 0.32 -0.2 5,179,856 + Pf6N2E2_4448 0.39 -1.1 5,179,857 - Pf6N2E2_4448 0.39 -0.9 5,180,061 - Pf6N2E2_4448 0.84 +0.8 5,180,176 - -0.8 5,180,264 - Pf6N2E2_4449 0.11 +0.2 5,180,264 - Pf6N2E2_4449 0.11 -0.2 5,180,280 + Pf6N2E2_4449 0.14 +1.8 5,180,280 + Pf6N2E2_4449 0.14 +0.8 5,180,280 + Pf6N2E2_4449 0.14 -0.2 5,180,280 + Pf6N2E2_4449 0.14 -0.2 5,180,280 + Pf6N2E2_4449 0.14 -0.6 5,180,280 + Pf6N2E2_4449 0.14 -1.0 5,180,280 + Pf6N2E2_4449 0.14 +0.7 5,180,281 - Pf6N2E2_4449 0.14 +1.3 5,180,281 - Pf6N2E2_4449 0.14 -0.4 5,180,281 - Pf6N2E2_4449 0.14 -0.5 5,180,281 - Pf6N2E2_4449 0.14 +0.1 5,180,421 + Pf6N2E2_4449 0.35 +0.5 5,180,422 - Pf6N2E2_4449 0.35 +0.6
Or see this region's nucleotide sequence