Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_4445

Experiment: Trisodium citrate (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_4443 and Pf6N2E2_4444 are separated by 200 nucleotidesPf6N2E2_4444 and Pf6N2E2_4445 are separated by 18 nucleotidesPf6N2E2_4445 and Pf6N2E2_4446 are separated by 303 nucleotidesPf6N2E2_4446 and Pf6N2E2_4447 are separated by 3 nucleotides Pf6N2E2_4443 - Lipoprotein YcfM, part of a salvage pathway of unknown substrate, at 5,174,786 to 5,175,373 _4443 Pf6N2E2_4444 - FIG00953537: hypothetical protein, at 5,175,574 to 5,175,945 _4444 Pf6N2E2_4445 - TPR domain protein, at 5,175,964 to 5,177,364 _4445 Pf6N2E2_4446 - hypothetical protein, at 5,177,668 to 5,177,781 _4446 Pf6N2E2_4447 - Cytochrome c family protein, at 5,177,785 to 5,179,572 _4447 Position (kb) 5175 5176 5177 5178Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3at 5174.985 kb on + strand, within Pf6N2E2_4443at 5175.097 kb on + strand, within Pf6N2E2_4443at 5175.120 kb on + strand, within Pf6N2E2_4443at 5175.120 kb on + strand, within Pf6N2E2_4443at 5175.121 kb on - strand, within Pf6N2E2_4443at 5175.121 kb on - strand, within Pf6N2E2_4443at 5175.121 kb on - strand, within Pf6N2E2_4443at 5175.121 kb on - strand, within Pf6N2E2_4443at 5175.147 kb on + strand, within Pf6N2E2_4443at 5175.148 kb on - strand, within Pf6N2E2_4443at 5175.148 kb on - strand, within Pf6N2E2_4443at 5175.279 kb on + strand, within Pf6N2E2_4443at 5175.279 kb on + strand, within Pf6N2E2_4443at 5175.280 kb on - strand, within Pf6N2E2_4443at 5175.366 kb on - strandat 5175.366 kb on - strandat 5175.366 kb on - strandat 5175.423 kb on + strandat 5175.424 kb on - strandat 5175.441 kb on + strandat 5175.442 kb on - strandat 5175.469 kb on + strandat 5175.470 kb on - strandat 5175.479 kb on - strandat 5175.523 kb on + strandat 5175.523 kb on + strandat 5175.523 kb on + strandat 5175.583 kb on - strandat 5175.728 kb on + strand, within Pf6N2E2_4444at 5175.731 kb on + strand, within Pf6N2E2_4444at 5175.731 kb on + strand, within Pf6N2E2_4444at 5175.731 kb on + strand, within Pf6N2E2_4444at 5175.731 kb on + strand, within Pf6N2E2_4444at 5175.731 kb on + strand, within Pf6N2E2_4444at 5175.731 kb on + strand, within Pf6N2E2_4444at 5175.732 kb on - strand, within Pf6N2E2_4444at 5175.732 kb on - strand, within Pf6N2E2_4444at 5175.732 kb on - strand, within Pf6N2E2_4444at 5175.732 kb on - strand, within Pf6N2E2_4444at 5175.732 kb on - strand, within Pf6N2E2_4444at 5175.732 kb on - strand, within Pf6N2E2_4444at 5175.732 kb on - strand, within Pf6N2E2_4444at 5175.749 kb on + strand, within Pf6N2E2_4444at 5175.749 kb on + strand, within Pf6N2E2_4444at 5175.749 kb on + strand, within Pf6N2E2_4444at 5175.749 kb on + strand, within Pf6N2E2_4444at 5175.750 kb on - strand, within Pf6N2E2_4444at 5175.750 kb on - strand, within Pf6N2E2_4444at 5175.938 kb on - strandat 5175.965 kb on + strandat 5175.965 kb on + strandat 5175.966 kb on - strandat 5175.966 kb on - strandat 5175.966 kb on - strandat 5176.175 kb on + strand, within Pf6N2E2_4445at 5176.175 kb on + strand, within Pf6N2E2_4445at 5176.176 kb on - strand, within Pf6N2E2_4445at 5176.716 kb on - strand, within Pf6N2E2_4445at 5176.716 kb on - strand, within Pf6N2E2_4445at 5176.805 kb on + strand, within Pf6N2E2_4445at 5176.806 kb on - strand, within Pf6N2E2_4445at 5176.806 kb on - strand, within Pf6N2E2_4445at 5177.013 kb on + strand, within Pf6N2E2_4445at 5177.014 kb on - strand, within Pf6N2E2_4445at 5177.048 kb on + strand, within Pf6N2E2_4445at 5177.049 kb on - strand, within Pf6N2E2_4445at 5177.049 kb on - strand, within Pf6N2E2_4445at 5177.049 kb on - strand, within Pf6N2E2_4445at 5177.171 kb on + strand, within Pf6N2E2_4445at 5177.171 kb on + strand, within Pf6N2E2_4445at 5177.172 kb on - strand, within Pf6N2E2_4445at 5177.172 kb on - strand, within Pf6N2E2_4445at 5177.174 kb on + strand, within Pf6N2E2_4445at 5177.174 kb on + strand, within Pf6N2E2_4445at 5177.174 kb on + strand, within Pf6N2E2_4445at 5177.174 kb on + strand, within Pf6N2E2_4445at 5177.174 kb on + strand, within Pf6N2E2_4445at 5177.174 kb on + strand, within Pf6N2E2_4445at 5177.175 kb on - strand, within Pf6N2E2_4445at 5177.175 kb on - strand, within Pf6N2E2_4445at 5177.175 kb on - strand, within Pf6N2E2_4445at 5177.175 kb on - strand, within Pf6N2E2_4445at 5177.175 kb on - strand, within Pf6N2E2_4445at 5177.282 kb on + strandat 5177.282 kb on + strandat 5177.430 kb on + strandat 5177.431 kb on - strandat 5177.464 kb on + strandat 5177.465 kb on - strandat 5177.498 kb on + strandat 5177.498 kb on + strandat 5177.498 kb on + strandat 5177.499 kb on - strandat 5177.511 kb on + strandat 5177.511 kb on + strandat 5177.511 kb on + strandat 5177.511 kb on + strandat 5177.511 kb on + strandat 5177.512 kb on - strandat 5177.515 kb on + strandat 5177.516 kb on - strandat 5177.709 kb on - strand, within Pf6N2E2_4446at 5177.783 kb on + strandat 5177.783 kb on + strandat 5177.783 kb on + strandat 5177.791 kb on + strandat 5177.791 kb on + strandat 5177.792 kb on - strandat 5178.034 kb on + strand, within Pf6N2E2_4447at 5178.034 kb on + strand, within Pf6N2E2_4447at 5178.034 kb on + strand, within Pf6N2E2_4447at 5178.034 kb on + strand, within Pf6N2E2_4447at 5178.034 kb on + strand, within Pf6N2E2_4447at 5178.034 kb on + strand, within Pf6N2E2_4447at 5178.034 kb on + strand, within Pf6N2E2_4447at 5178.035 kb on - strand, within Pf6N2E2_4447at 5178.035 kb on - strand, within Pf6N2E2_4447at 5178.035 kb on - strand, within Pf6N2E2_4447at 5178.035 kb on - strand, within Pf6N2E2_4447at 5178.091 kb on + strand, within Pf6N2E2_4447at 5178.091 kb on + strand, within Pf6N2E2_4447at 5178.091 kb on + strand, within Pf6N2E2_4447at 5178.214 kb on + strand, within Pf6N2E2_4447at 5178.214 kb on + strand, within Pf6N2E2_4447at 5178.215 kb on - strand, within Pf6N2E2_4447at 5178.223 kb on + strand, within Pf6N2E2_4447at 5178.223 kb on + strand, within Pf6N2E2_4447at 5178.223 kb on + strand, within Pf6N2E2_4447at 5178.223 kb on + strand, within Pf6N2E2_4447at 5178.223 kb on + strand, within Pf6N2E2_4447at 5178.224 kb on - strand, within Pf6N2E2_4447at 5178.224 kb on - strand, within Pf6N2E2_4447at 5178.224 kb on - strand, within Pf6N2E2_4447at 5178.224 kb on - strand, within Pf6N2E2_4447at 5178.224 kb on - strand, within Pf6N2E2_4447at 5178.224 kb on - strand, within Pf6N2E2_4447at 5178.258 kb on + strand, within Pf6N2E2_4447at 5178.258 kb on + strand, within Pf6N2E2_4447at 5178.258 kb on + strand, within Pf6N2E2_4447at 5178.259 kb on - strand, within Pf6N2E2_4447at 5178.313 kb on + strand, within Pf6N2E2_4447at 5178.313 kb on + strand, within Pf6N2E2_4447at 5178.313 kb on + strand, within Pf6N2E2_4447at 5178.313 kb on + strand, within Pf6N2E2_4447at 5178.313 kb on + strand, within Pf6N2E2_4447at 5178.314 kb on - strand, within Pf6N2E2_4447at 5178.314 kb on - strand, within Pf6N2E2_4447at 5178.314 kb on - strand, within Pf6N2E2_4447at 5178.314 kb on - strand, within Pf6N2E2_4447at 5178.314 kb on - strand, within Pf6N2E2_4447at 5178.314 kb on - strand, within Pf6N2E2_4447

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Per-strain Table

Position Strand Gene LocusTag Fraction Trisodium citrate (C)
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5,174,985 + Pf6N2E2_4443 0.34 +0.1
5,175,097 + Pf6N2E2_4443 0.53 +0.2
5,175,120 + Pf6N2E2_4443 0.57 +1.0
5,175,120 + Pf6N2E2_4443 0.57 -0.3
5,175,121 - Pf6N2E2_4443 0.57 +1.9
5,175,121 - Pf6N2E2_4443 0.57 +0.4
5,175,121 - Pf6N2E2_4443 0.57 -1.2
5,175,121 - Pf6N2E2_4443 0.57 -1.2
5,175,147 + Pf6N2E2_4443 0.61 -0.1
5,175,148 - Pf6N2E2_4443 0.62 +0.5
5,175,148 - Pf6N2E2_4443 0.62 +0.2
5,175,279 + Pf6N2E2_4443 0.84 +0.5
5,175,279 + Pf6N2E2_4443 0.84 -0.3
5,175,280 - Pf6N2E2_4443 0.84 +0.9
5,175,366 - -0.7
5,175,366 - +0.1
5,175,366 - -1.1
5,175,423 + -0.0
5,175,424 - -0.3
5,175,441 + +0.1
5,175,442 - +1.0
5,175,469 + +0.4
5,175,470 - +0.3
5,175,479 - +0.5
5,175,523 + +1.0
5,175,523 + -0.6
5,175,523 + -0.0
5,175,583 - -1.7
5,175,728 + Pf6N2E2_4444 0.41 +0.9
5,175,731 + Pf6N2E2_4444 0.42 +0.0
5,175,731 + Pf6N2E2_4444 0.42 -0.8
5,175,731 + Pf6N2E2_4444 0.42 +1.4
5,175,731 + Pf6N2E2_4444 0.42 -0.0
5,175,731 + Pf6N2E2_4444 0.42 -0.5
5,175,731 + Pf6N2E2_4444 0.42 +1.0
5,175,732 - Pf6N2E2_4444 0.42 +0.1
5,175,732 - Pf6N2E2_4444 0.42 -0.5
5,175,732 - Pf6N2E2_4444 0.42 +0.1
5,175,732 - Pf6N2E2_4444 0.42 -0.2
5,175,732 - Pf6N2E2_4444 0.42 -0.2
5,175,732 - Pf6N2E2_4444 0.42 +0.8
5,175,732 - Pf6N2E2_4444 0.42 -0.4
5,175,749 + Pf6N2E2_4444 0.47 -0.2
5,175,749 + Pf6N2E2_4444 0.47 +0.6
5,175,749 + Pf6N2E2_4444 0.47 +0.4
5,175,749 + Pf6N2E2_4444 0.47 +0.5
5,175,750 - Pf6N2E2_4444 0.47 -0.2
5,175,750 - Pf6N2E2_4444 0.47 +1.0
5,175,938 - +0.4
5,175,965 + +0.6
5,175,965 + -0.2
5,175,966 - -0.4
5,175,966 - +0.4
5,175,966 - +1.2
5,176,175 + Pf6N2E2_4445 0.15 +0.3
5,176,175 + Pf6N2E2_4445 0.15 +1.2
5,176,176 - Pf6N2E2_4445 0.15 +0.1
5,176,716 - Pf6N2E2_4445 0.54 +0.3
5,176,716 - Pf6N2E2_4445 0.54 -0.1
5,176,805 + Pf6N2E2_4445 0.60 -0.4
5,176,806 - Pf6N2E2_4445 0.60 -0.5
5,176,806 - Pf6N2E2_4445 0.60 +2.4
5,177,013 + Pf6N2E2_4445 0.75 +0.6
5,177,014 - Pf6N2E2_4445 0.75 +1.0
5,177,048 + Pf6N2E2_4445 0.77 +0.8
5,177,049 - Pf6N2E2_4445 0.77 +1.0
5,177,049 - Pf6N2E2_4445 0.77 -0.2
5,177,049 - Pf6N2E2_4445 0.77 -0.5
5,177,171 + Pf6N2E2_4445 0.86 -0.4
5,177,171 + Pf6N2E2_4445 0.86 +1.8
5,177,172 - Pf6N2E2_4445 0.86 -1.1
5,177,172 - Pf6N2E2_4445 0.86 -0.8
5,177,174 + Pf6N2E2_4445 0.86 +0.7
5,177,174 + Pf6N2E2_4445 0.86 +1.0
5,177,174 + Pf6N2E2_4445 0.86 +0.5
5,177,174 + Pf6N2E2_4445 0.86 +1.6
5,177,174 + Pf6N2E2_4445 0.86 +1.1
5,177,174 + Pf6N2E2_4445 0.86 +1.0
5,177,175 - Pf6N2E2_4445 0.86 -0.5
5,177,175 - Pf6N2E2_4445 0.86 +0.2
5,177,175 - Pf6N2E2_4445 0.86 -1.0
5,177,175 - Pf6N2E2_4445 0.86 -1.1
5,177,175 - Pf6N2E2_4445 0.86 +1.0
5,177,282 + +1.4
5,177,282 + +1.6
5,177,430 + +2.8
5,177,431 - +0.6
5,177,464 + +2.5
5,177,465 - -1.0
5,177,498 + +2.3
5,177,498 + +1.3
5,177,498 + +1.4
5,177,499 - +0.0
5,177,511 + +0.5
5,177,511 + +0.3
5,177,511 + +1.5
5,177,511 + +0.3
5,177,511 + +1.6
5,177,512 - -1.7
5,177,515 + +1.0
5,177,516 - -1.6
5,177,709 - Pf6N2E2_4446 0.36 -2.5
5,177,783 + -2.8
5,177,783 + -3.3
5,177,783 + -1.2
5,177,791 + -1.0
5,177,791 + -2.4
5,177,792 - -1.2
5,178,034 + Pf6N2E2_4447 0.14 -2.2
5,178,034 + Pf6N2E2_4447 0.14 -1.6
5,178,034 + Pf6N2E2_4447 0.14 -1.3
5,178,034 + Pf6N2E2_4447 0.14 -3.1
5,178,034 + Pf6N2E2_4447 0.14 -2.8
5,178,034 + Pf6N2E2_4447 0.14 -1.4
5,178,034 + Pf6N2E2_4447 0.14 -2.5
5,178,035 - Pf6N2E2_4447 0.14 -2.9
5,178,035 - Pf6N2E2_4447 0.14 -1.7
5,178,035 - Pf6N2E2_4447 0.14 -2.2
5,178,035 - Pf6N2E2_4447 0.14 -2.3
5,178,091 + Pf6N2E2_4447 0.17 -3.5
5,178,091 + Pf6N2E2_4447 0.17 -2.2
5,178,091 + Pf6N2E2_4447 0.17 -2.9
5,178,214 + Pf6N2E2_4447 0.24 -2.5
5,178,214 + Pf6N2E2_4447 0.24 -1.9
5,178,215 - Pf6N2E2_4447 0.24 -2.7
5,178,223 + Pf6N2E2_4447 0.24 -1.8
5,178,223 + Pf6N2E2_4447 0.24 -0.9
5,178,223 + Pf6N2E2_4447 0.24 -3.8
5,178,223 + Pf6N2E2_4447 0.24 -0.5
5,178,223 + Pf6N2E2_4447 0.24 -2.3
5,178,224 - Pf6N2E2_4447 0.25 -1.9
5,178,224 - Pf6N2E2_4447 0.25 -3.1
5,178,224 - Pf6N2E2_4447 0.25 -3.2
5,178,224 - Pf6N2E2_4447 0.25 -1.7
5,178,224 - Pf6N2E2_4447 0.25 -0.2
5,178,224 - Pf6N2E2_4447 0.25 -2.7
5,178,258 + Pf6N2E2_4447 0.26 -3.1
5,178,258 + Pf6N2E2_4447 0.26 -2.3
5,178,258 + Pf6N2E2_4447 0.26 -3.9
5,178,259 - Pf6N2E2_4447 0.27 -3.5
5,178,313 + Pf6N2E2_4447 0.30 -1.5
5,178,313 + Pf6N2E2_4447 0.30 -0.9
5,178,313 + Pf6N2E2_4447 0.30 -3.1
5,178,313 + Pf6N2E2_4447 0.30 -1.4
5,178,313 + Pf6N2E2_4447 0.30 -1.1
5,178,314 - Pf6N2E2_4447 0.30 -3.1
5,178,314 - Pf6N2E2_4447 0.30 -2.0
5,178,314 - Pf6N2E2_4447 0.30 -2.1
5,178,314 - Pf6N2E2_4447 0.30 -2.8
5,178,314 - Pf6N2E2_4447 0.30 -1.5
5,178,314 - Pf6N2E2_4447 0.30 +1.0

Or see this region's nucleotide sequence