Experiment: Trisodium citrate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3653 and Pf6N2E2_3654 are separated by 85 nucleotides Pf6N2E2_3654 and Pf6N2E2_3655 are separated by 268 nucleotides
Pf6N2E2_3653 - ADP-heptose synthase (EC 2.7.-.-) / D-glycero-beta-D-manno-heptose 7-phosphate kinase, at 4,253,445 to 4,254,866
_3653
Pf6N2E2_3654 - Adenylylsulfate kinase (EC 2.7.1.25), at 4,254,952 to 4,255,413
_3654
Pf6N2E2_3655 - Hypothetical protein FIG015671 in large core OS assembly cluster, at 4,255,682 to 4,257,097
_3655
Position (kb)
4254
4255
4256 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4254.881 kb on - strand at 4254.906 kb on - strand at 4254.906 kb on - strand at 4254.906 kb on - strand at 4254.906 kb on - strand at 4254.906 kb on - strand at 4255.046 kb on + strand, within Pf6N2E2_3654 at 4255.047 kb on - strand, within Pf6N2E2_3654 at 4255.100 kb on + strand, within Pf6N2E2_3654 at 4255.151 kb on + strand, within Pf6N2E2_3654 at 4255.151 kb on + strand, within Pf6N2E2_3654 at 4255.151 kb on + strand, within Pf6N2E2_3654 at 4255.152 kb on - strand, within Pf6N2E2_3654 at 4255.152 kb on - strand, within Pf6N2E2_3654 at 4255.330 kb on + strand, within Pf6N2E2_3654 at 4255.330 kb on + strand, within Pf6N2E2_3654 at 4255.330 kb on + strand, within Pf6N2E2_3654 at 4255.330 kb on + strand, within Pf6N2E2_3654 at 4255.331 kb on - strand, within Pf6N2E2_3654 at 4255.331 kb on - strand, within Pf6N2E2_3654 at 4255.378 kb on + strand at 4255.379 kb on - strand at 4255.452 kb on + strand at 4255.453 kb on - strand at 4255.453 kb on - strand at 4255.453 kb on - strand at 4255.521 kb on + strand at 4255.540 kb on + strand at 4255.624 kb on + strand at 4255.624 kb on + strand at 4255.626 kb on + strand at 4255.634 kb on + strand at 4255.653 kb on + strand at 4255.653 kb on + strand at 4255.654 kb on - strand at 4255.654 kb on - strand at 4255.866 kb on - strand, within Pf6N2E2_3655 at 4255.916 kb on + strand at 4256.016 kb on + strand, within Pf6N2E2_3655 at 4256.017 kb on - strand, within Pf6N2E2_3655 at 4256.017 kb on - strand, within Pf6N2E2_3655 at 4256.017 kb on - strand, within Pf6N2E2_3655 at 4256.017 kb on - strand, within Pf6N2E2_3655 at 4256.029 kb on + strand, within Pf6N2E2_3655 at 4256.044 kb on + strand, within Pf6N2E2_3655 at 4256.045 kb on - strand, within Pf6N2E2_3655 at 4256.045 kb on - strand, within Pf6N2E2_3655 at 4256.160 kb on - strand, within Pf6N2E2_3655 at 4256.160 kb on - strand, within Pf6N2E2_3655 at 4256.248 kb on + strand, within Pf6N2E2_3655 at 4256.249 kb on - strand, within Pf6N2E2_3655 at 4256.249 kb on - strand, within Pf6N2E2_3655 at 4256.255 kb on - strand, within Pf6N2E2_3655 at 4256.322 kb on + strand, within Pf6N2E2_3655 at 4256.322 kb on + strand, within Pf6N2E2_3655 at 4256.322 kb on + strand, within Pf6N2E2_3655 at 4256.322 kb on + strand, within Pf6N2E2_3655 at 4256.322 kb on + strand, within Pf6N2E2_3655 at 4256.323 kb on - strand, within Pf6N2E2_3655 at 4256.323 kb on - strand, within Pf6N2E2_3655 at 4256.323 kb on - strand, within Pf6N2E2_3655 at 4256.325 kb on + strand, within Pf6N2E2_3655
Per-strain Table
Position Strand Gene LocusTag Fraction Trisodium citrate (C) remove 4,254,881 - +0.5 4,254,906 - -0.6 4,254,906 - +0.3 4,254,906 - +0.1 4,254,906 - -0.1 4,254,906 - -0.4 4,255,046 + Pf6N2E2_3654 0.20 +0.2 4,255,047 - Pf6N2E2_3654 0.21 +0.0 4,255,100 + Pf6N2E2_3654 0.32 +0.3 4,255,151 + Pf6N2E2_3654 0.43 -0.7 4,255,151 + Pf6N2E2_3654 0.43 -0.2 4,255,151 + Pf6N2E2_3654 0.43 -0.5 4,255,152 - Pf6N2E2_3654 0.43 -0.0 4,255,152 - Pf6N2E2_3654 0.43 -0.7 4,255,330 + Pf6N2E2_3654 0.82 +1.1 4,255,330 + Pf6N2E2_3654 0.82 -0.0 4,255,330 + Pf6N2E2_3654 0.82 +0.3 4,255,330 + Pf6N2E2_3654 0.82 +0.8 4,255,331 - Pf6N2E2_3654 0.82 -0.2 4,255,331 - Pf6N2E2_3654 0.82 -2.5 4,255,378 + +1.0 4,255,379 - -0.4 4,255,452 + -1.0 4,255,453 - +0.2 4,255,453 - +0.3 4,255,453 - +0.6 4,255,521 + -1.7 4,255,540 + -1.1 4,255,624 + -0.7 4,255,624 + -0.8 4,255,626 + +0.4 4,255,634 + +0.5 4,255,653 + -0.5 4,255,653 + +0.7 4,255,654 - +0.2 4,255,654 - +0.3 4,255,866 - Pf6N2E2_3655 0.13 +1.2 4,255,916 + +0.8 4,256,016 + Pf6N2E2_3655 0.24 +1.3 4,256,017 - Pf6N2E2_3655 0.24 +0.1 4,256,017 - Pf6N2E2_3655 0.24 +1.1 4,256,017 - Pf6N2E2_3655 0.24 +1.3 4,256,017 - Pf6N2E2_3655 0.24 +1.3 4,256,029 + Pf6N2E2_3655 0.25 +1.9 4,256,044 + Pf6N2E2_3655 0.26 +0.8 4,256,045 - Pf6N2E2_3655 0.26 +1.0 4,256,045 - Pf6N2E2_3655 0.26 +1.0 4,256,160 - Pf6N2E2_3655 0.34 +1.2 4,256,160 - Pf6N2E2_3655 0.34 +0.6 4,256,248 + Pf6N2E2_3655 0.40 +1.4 4,256,249 - Pf6N2E2_3655 0.40 +1.1 4,256,249 - Pf6N2E2_3655 0.40 +0.3 4,256,255 - Pf6N2E2_3655 0.40 +1.4 4,256,322 + Pf6N2E2_3655 0.45 +1.3 4,256,322 + Pf6N2E2_3655 0.45 +1.8 4,256,322 + Pf6N2E2_3655 0.45 +0.3 4,256,322 + Pf6N2E2_3655 0.45 +2.0 4,256,322 + Pf6N2E2_3655 0.45 +0.3 4,256,323 - Pf6N2E2_3655 0.45 +2.1 4,256,323 - Pf6N2E2_3655 0.45 +1.0 4,256,323 - Pf6N2E2_3655 0.45 +0.9 4,256,325 + Pf6N2E2_3655 0.45 +2.3
Or see this region's nucleotide sequence