Experiment: Trisodium citrate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3142 and Pf6N2E2_3143 are separated by 104 nucleotides Pf6N2E2_3143 and Pf6N2E2_3144 are separated by 132 nucleotides
Pf6N2E2_3142 - Threonine synthase (EC 4.2.3.1), at 3,634,653 to 3,636,062
_3142
Pf6N2E2_3143 - Homoserine dehydrogenase (EC 1.1.1.3), at 3,636,167 to 3,637,471
_3143
Pf6N2E2_3144 - Thiol:disulfide interchange protein DsbC, at 3,637,604 to 3,638,335
_3144
Position (kb)
3636
3637
3638 Strain fitness (log2 ratio)
-1
0
1 at 3636.169 kb on - strand at 3637.517 kb on - strand at 3637.879 kb on - strand, within Pf6N2E2_3144 at 3637.879 kb on - strand, within Pf6N2E2_3144 at 3637.879 kb on - strand, within Pf6N2E2_3144 at 3637.931 kb on - strand, within Pf6N2E2_3144 at 3637.931 kb on - strand, within Pf6N2E2_3144 at 3637.939 kb on - strand, within Pf6N2E2_3144 at 3637.999 kb on - strand, within Pf6N2E2_3144 at 3638.122 kb on - strand, within Pf6N2E2_3144 at 3638.122 kb on - strand, within Pf6N2E2_3144 at 3638.122 kb on - strand, within Pf6N2E2_3144 at 3638.122 kb on - strand, within Pf6N2E2_3144 at 3638.122 kb on - strand, within Pf6N2E2_3144 at 3638.122 kb on - strand, within Pf6N2E2_3144 at 3638.122 kb on - strand, within Pf6N2E2_3144 at 3638.122 kb on - strand, within Pf6N2E2_3144 at 3638.122 kb on - strand, within Pf6N2E2_3144 at 3638.122 kb on - strand, within Pf6N2E2_3144 at 3638.143 kb on - strand, within Pf6N2E2_3144 at 3638.176 kb on - strand, within Pf6N2E2_3144 at 3638.176 kb on - strand, within Pf6N2E2_3144 at 3638.430 kb on + strand at 3638.430 kb on + strand at 3638.430 kb on + strand at 3638.430 kb on + strand at 3638.430 kb on + strand at 3638.431 kb on - strand at 3638.431 kb on - strand at 3638.431 kb on - strand at 3638.431 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Trisodium citrate (C) remove 3,636,169 - +0.1 3,637,517 - +0.6 3,637,879 - Pf6N2E2_3144 0.38 +1.1 3,637,879 - Pf6N2E2_3144 0.38 +0.9 3,637,879 - Pf6N2E2_3144 0.38 -0.2 3,637,931 - Pf6N2E2_3144 0.45 -0.4 3,637,931 - Pf6N2E2_3144 0.45 +0.4 3,637,939 - Pf6N2E2_3144 0.46 +0.4 3,637,999 - Pf6N2E2_3144 0.54 +0.4 3,638,122 - Pf6N2E2_3144 0.71 +1.5 3,638,122 - Pf6N2E2_3144 0.71 +1.2 3,638,122 - Pf6N2E2_3144 0.71 +0.6 3,638,122 - Pf6N2E2_3144 0.71 -0.3 3,638,122 - Pf6N2E2_3144 0.71 +0.4 3,638,122 - Pf6N2E2_3144 0.71 -0.4 3,638,122 - Pf6N2E2_3144 0.71 +1.1 3,638,122 - Pf6N2E2_3144 0.71 -0.4 3,638,122 - Pf6N2E2_3144 0.71 +0.6 3,638,122 - Pf6N2E2_3144 0.71 +0.8 3,638,143 - Pf6N2E2_3144 0.74 +0.7 3,638,176 - Pf6N2E2_3144 0.78 +0.1 3,638,176 - Pf6N2E2_3144 0.78 +0.9 3,638,430 + -0.7 3,638,430 + -1.3 3,638,430 + -0.9 3,638,430 + +0.7 3,638,430 + +0.0 3,638,431 - +1.3 3,638,431 - -0.5 3,638,431 - +0.1 3,638,431 - +0.5
Or see this region's nucleotide sequence