Experiment: Trisodium citrate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1993 and Pf6N2E2_1994 are separated by 4 nucleotides Pf6N2E2_1994 and Pf6N2E2_1995 overlap by 1 nucleotides Pf6N2E2_1995 and Pf6N2E2_1996 are separated by 809 nucleotides
Pf6N2E2_1993 - putative exported protein, at 2,356,889 to 2,358,316
_1993
Pf6N2E2_1994 - putative lipoprotein, at 2,358,321 to 2,358,719
_1994
Pf6N2E2_1995 - Type IV secretory pathway, VirB4 components, at 2,358,719 to 2,361,568
_1995
Pf6N2E2_1996 - DNA repair protein RadC, at 2,362,378 to 2,362,872
_1996
Position (kb)
2358
2359
2360
2361
2362 Strain fitness (log2 ratio)
-2
-1
0
1 at 2357.749 kb on + strand, within Pf6N2E2_1993 at 2357.812 kb on - strand, within Pf6N2E2_1993 at 2357.975 kb on + strand, within Pf6N2E2_1993 at 2358.054 kb on - strand, within Pf6N2E2_1993 at 2358.167 kb on + strand, within Pf6N2E2_1993 at 2358.192 kb on - strand at 2358.239 kb on - strand at 2358.534 kb on - strand, within Pf6N2E2_1994 at 2358.600 kb on + strand, within Pf6N2E2_1994 at 2358.600 kb on + strand, within Pf6N2E2_1994 at 2358.601 kb on - strand, within Pf6N2E2_1994 at 2358.648 kb on + strand, within Pf6N2E2_1994 at 2358.684 kb on + strand at 2358.714 kb on + strand at 2358.746 kb on + strand at 2358.746 kb on + strand at 2358.746 kb on + strand at 2358.746 kb on + strand at 2358.747 kb on - strand at 2358.747 kb on - strand at 2358.931 kb on - strand at 2359.111 kb on - strand, within Pf6N2E2_1995 at 2359.111 kb on - strand, within Pf6N2E2_1995 at 2359.111 kb on - strand, within Pf6N2E2_1995 at 2359.111 kb on - strand, within Pf6N2E2_1995 at 2359.113 kb on - strand, within Pf6N2E2_1995 at 2359.113 kb on - strand, within Pf6N2E2_1995 at 2359.113 kb on - strand, within Pf6N2E2_1995 at 2359.113 kb on - strand, within Pf6N2E2_1995 at 2359.151 kb on - strand, within Pf6N2E2_1995 at 2359.164 kb on - strand, within Pf6N2E2_1995 at 2359.206 kb on - strand, within Pf6N2E2_1995 at 2359.210 kb on - strand, within Pf6N2E2_1995 at 2359.536 kb on - strand, within Pf6N2E2_1995 at 2359.766 kb on + strand, within Pf6N2E2_1995 at 2359.767 kb on - strand, within Pf6N2E2_1995 at 2359.831 kb on - strand, within Pf6N2E2_1995 at 2359.831 kb on - strand, within Pf6N2E2_1995 at 2359.975 kb on - strand, within Pf6N2E2_1995 at 2360.087 kb on - strand, within Pf6N2E2_1995 at 2360.087 kb on - strand, within Pf6N2E2_1995 at 2360.087 kb on - strand, within Pf6N2E2_1995 at 2360.470 kb on - strand, within Pf6N2E2_1995 at 2360.494 kb on - strand, within Pf6N2E2_1995 at 2360.701 kb on + strand, within Pf6N2E2_1995 at 2361.062 kb on - strand, within Pf6N2E2_1995 at 2361.116 kb on + strand, within Pf6N2E2_1995 at 2361.117 kb on - strand, within Pf6N2E2_1995 at 2361.117 kb on - strand, within Pf6N2E2_1995 at 2361.344 kb on + strand at 2361.403 kb on + strand at 2361.404 kb on - strand at 2361.410 kb on + strand at 2361.410 kb on + strand at 2361.410 kb on + strand at 2361.414 kb on + strand at 2361.415 kb on - strand at 2361.519 kb on + strand at 2361.596 kb on + strand at 2361.599 kb on + strand at 2361.600 kb on - strand at 2361.692 kb on + strand at 2361.692 kb on + strand at 2361.696 kb on + strand at 2361.696 kb on + strand at 2361.730 kb on + strand at 2361.788 kb on + strand at 2361.789 kb on - strand at 2362.006 kb on + strand at 2362.006 kb on + strand at 2362.007 kb on - strand at 2362.026 kb on + strand at 2362.175 kb on + strand at 2362.175 kb on + strand at 2362.309 kb on + strand at 2362.309 kb on + strand at 2362.309 kb on + strand at 2362.310 kb on - strand at 2362.310 kb on - strand at 2362.310 kb on - strand at 2362.432 kb on - strand, within Pf6N2E2_1996 at 2362.482 kb on + strand, within Pf6N2E2_1996 at 2362.482 kb on + strand, within Pf6N2E2_1996 at 2362.482 kb on + strand, within Pf6N2E2_1996 at 2362.482 kb on + strand, within Pf6N2E2_1996 at 2362.482 kb on + strand, within Pf6N2E2_1996 at 2362.482 kb on + strand, within Pf6N2E2_1996 at 2362.483 kb on - strand, within Pf6N2E2_1996 at 2362.483 kb on - strand, within Pf6N2E2_1996 at 2362.483 kb on - strand, within Pf6N2E2_1996 at 2362.501 kb on + strand, within Pf6N2E2_1996 at 2362.535 kb on + strand, within Pf6N2E2_1996 at 2362.536 kb on - strand, within Pf6N2E2_1996
Per-strain Table
Position Strand Gene LocusTag Fraction Trisodium citrate (C) remove 2,357,749 + Pf6N2E2_1993 0.60 -0.0 2,357,812 - Pf6N2E2_1993 0.65 -0.0 2,357,975 + Pf6N2E2_1993 0.76 +1.2 2,358,054 - Pf6N2E2_1993 0.82 -0.1 2,358,167 + Pf6N2E2_1993 0.89 +0.9 2,358,192 - +0.2 2,358,239 - +1.0 2,358,534 - Pf6N2E2_1994 0.53 +0.3 2,358,600 + Pf6N2E2_1994 0.70 -0.4 2,358,600 + Pf6N2E2_1994 0.70 -0.3 2,358,601 - Pf6N2E2_1994 0.70 +0.7 2,358,648 + Pf6N2E2_1994 0.82 +0.6 2,358,684 + +0.3 2,358,714 + +0.1 2,358,746 + +0.3 2,358,746 + +0.0 2,358,746 + +0.1 2,358,746 + +1.0 2,358,747 - -0.7 2,358,747 - -0.1 2,358,931 - -0.7 2,359,111 - Pf6N2E2_1995 0.14 -0.1 2,359,111 - Pf6N2E2_1995 0.14 -0.9 2,359,111 - Pf6N2E2_1995 0.14 -1.2 2,359,111 - Pf6N2E2_1995 0.14 +1.5 2,359,113 - Pf6N2E2_1995 0.14 -0.4 2,359,113 - Pf6N2E2_1995 0.14 -0.9 2,359,113 - Pf6N2E2_1995 0.14 -1.7 2,359,113 - Pf6N2E2_1995 0.14 -0.2 2,359,151 - Pf6N2E2_1995 0.15 +0.6 2,359,164 - Pf6N2E2_1995 0.16 -0.5 2,359,206 - Pf6N2E2_1995 0.17 +0.2 2,359,210 - Pf6N2E2_1995 0.17 -0.8 2,359,536 - Pf6N2E2_1995 0.29 +0.3 2,359,766 + Pf6N2E2_1995 0.37 +1.6 2,359,767 - Pf6N2E2_1995 0.37 -0.7 2,359,831 - Pf6N2E2_1995 0.39 +0.6 2,359,831 - Pf6N2E2_1995 0.39 -2.2 2,359,975 - Pf6N2E2_1995 0.44 +0.2 2,360,087 - Pf6N2E2_1995 0.48 -0.5 2,360,087 - Pf6N2E2_1995 0.48 -0.0 2,360,087 - Pf6N2E2_1995 0.48 -1.1 2,360,470 - Pf6N2E2_1995 0.61 -2.2 2,360,494 - Pf6N2E2_1995 0.62 -1.3 2,360,701 + Pf6N2E2_1995 0.70 -1.6 2,361,062 - Pf6N2E2_1995 0.82 -0.5 2,361,116 + Pf6N2E2_1995 0.84 +0.1 2,361,117 - Pf6N2E2_1995 0.84 +0.4 2,361,117 - Pf6N2E2_1995 0.84 -0.4 2,361,344 + -0.6 2,361,403 + -0.9 2,361,404 - -0.6 2,361,410 + -0.2 2,361,410 + -1.3 2,361,410 + +0.1 2,361,414 + -1.6 2,361,415 - -0.3 2,361,519 + +0.5 2,361,596 + -0.8 2,361,599 + -1.2 2,361,600 - -0.3 2,361,692 + -1.2 2,361,692 + -1.3 2,361,696 + +1.0 2,361,696 + -0.5 2,361,730 + +0.3 2,361,788 + -0.2 2,361,789 - +0.7 2,362,006 + -0.6 2,362,006 + +0.8 2,362,007 - -0.6 2,362,026 + -0.1 2,362,175 + +0.2 2,362,175 + -1.1 2,362,309 + -0.4 2,362,309 + -0.1 2,362,309 + +0.4 2,362,310 - -1.8 2,362,310 - -0.9 2,362,310 - +1.0 2,362,432 - Pf6N2E2_1996 0.11 +1.5 2,362,482 + Pf6N2E2_1996 0.21 -0.0 2,362,482 + Pf6N2E2_1996 0.21 -0.3 2,362,482 + Pf6N2E2_1996 0.21 +0.6 2,362,482 + Pf6N2E2_1996 0.21 +0.1 2,362,482 + Pf6N2E2_1996 0.21 -1.3 2,362,482 + Pf6N2E2_1996 0.21 -0.7 2,362,483 - Pf6N2E2_1996 0.21 -0.4 2,362,483 - Pf6N2E2_1996 0.21 +0.2 2,362,483 - Pf6N2E2_1996 0.21 +0.9 2,362,501 + Pf6N2E2_1996 0.25 +0.3 2,362,535 + Pf6N2E2_1996 0.32 +0.3 2,362,536 - Pf6N2E2_1996 0.32 +0.7
Or see this region's nucleotide sequence