Experiment: Formate/Acetate-Sulfate (60/30-60 mM)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt trpG and trpD overlap by 4 nucleotides trpD and trpC overlap by 8 nucleotides trpC and trpF-1 are separated by 47 nucleotides trpF-1 and trpB-2 overlap by 4 nucleotides
DVU0466: trpG - anthranilate synthase, glutamine amidotransferase component (TIGR), at 534,432 to 535,061
trpG
DVU0467: trpD - anthranilate phosphoribosyltransferase (TIGR), at 535,058 to 536,056
trpD
DVU0468: trpC - indole-3-glycerol phosphate synthase (TIGR), at 536,049 to 536,822
trpC
DVU0469: trpF-1 - N-(5-phosphoribosyl)anthranilate isomerase (TIGR), at 536,870 to 537,724
trpF-1
DVU0470: trpB-2 - tryptophan synthase, beta subunit (TIGR), at 537,721 to 538,908
trpB-2
Position (kb) 536
537 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 535.103 kb on + strand at 535.130 kb on + strand at 535.204 kb on + strand, within trpD at 535.206 kb on - strand, within trpD at 535.229 kb on - strand, within trpD at 535.233 kb on - strand, within trpD at 535.239 kb on + strand, within trpD at 535.244 kb on + strand, within trpD at 535.244 kb on + strand, within trpD at 535.244 kb on + strand, within trpD at 535.244 kb on + strand, within trpD at 535.244 kb on + strand, within trpD at 535.252 kb on - strand, within trpD at 535.252 kb on - strand, within trpD at 535.252 kb on - strand, within trpD at 535.252 kb on - strand, within trpD at 535.257 kb on - strand, within trpD at 535.272 kb on + strand, within trpD at 535.447 kb on - strand, within trpD at 535.508 kb on - strand, within trpD at 535.546 kb on + strand, within trpD at 535.591 kb on - strand, within trpD at 535.634 kb on - strand, within trpD at 535.709 kb on + strand, within trpD at 535.756 kb on - strand, within trpD at 535.839 kb on + strand, within trpD at 535.841 kb on + strand, within trpD at 535.841 kb on + strand, within trpD at 535.849 kb on - strand, within trpD at 535.871 kb on - strand, within trpD at 535.897 kb on + strand, within trpD at 535.905 kb on - strand, within trpD at 535.920 kb on - strand, within trpD at 535.938 kb on + strand, within trpD at 535.942 kb on - strand, within trpD at 535.942 kb on - strand, within trpD at 535.955 kb on + strand, within trpD at 535.955 kb on + strand, within trpD at 535.963 kb on - strand at 535.983 kb on + strand at 535.985 kb on + strand at 535.985 kb on + strand at 535.985 kb on + strand at 536.095 kb on - strand at 536.158 kb on + strand, within trpC at 536.164 kb on - strand, within trpC at 536.166 kb on - strand, within trpC at 536.166 kb on - strand, within trpC at 536.179 kb on + strand, within trpC at 536.179 kb on + strand, within trpC at 536.179 kb on + strand, within trpC at 536.301 kb on - strand, within trpC at 536.303 kb on + strand, within trpC at 536.311 kb on - strand, within trpC at 536.318 kb on + strand, within trpC at 536.318 kb on + strand, within trpC at 536.369 kb on - strand, within trpC at 536.425 kb on + strand, within trpC at 536.461 kb on + strand, within trpC at 536.467 kb on + strand, within trpC at 536.506 kb on - strand, within trpC at 536.511 kb on + strand, within trpC at 536.519 kb on - strand, within trpC at 536.519 kb on - strand, within trpC at 536.547 kb on - strand, within trpC at 536.566 kb on + strand, within trpC at 536.611 kb on + strand, within trpC at 536.619 kb on - strand, within trpC at 536.669 kb on + strand, within trpC at 536.750 kb on - strand at 536.757 kb on + strand at 536.757 kb on + strand at 536.765 kb on - strand at 536.799 kb on + strand at 536.807 kb on - strand at 536.860 kb on + strand at 536.979 kb on + strand, within trpF-1 at 537.037 kb on - strand, within trpF-1 at 537.037 kb on - strand, within trpF-1 at 537.044 kb on - strand, within trpF-1 at 537.044 kb on - strand, within trpF-1 at 537.135 kb on + strand, within trpF-1 at 537.206 kb on - strand, within trpF-1 at 537.288 kb on + strand, within trpF-1 at 537.389 kb on - strand, within trpF-1 at 537.476 kb on + strand, within trpF-1 at 537.479 kb on - strand, within trpF-1 at 537.489 kb on - strand, within trpF-1 at 537.574 kb on - strand, within trpF-1 at 537.579 kb on - strand, within trpF-1 at 537.664 kb on - strand at 537.671 kb on - strand at 537.688 kb on - strand at 537.690 kb on + strand at 537.692 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Formate/Acetate-Sulfate (60/30-60 mM) remove 535,103 + -1.7 535,130 + -1.7 535,204 + trpD DVU0467 0.15 -1.1 535,206 - trpD DVU0467 0.15 -0.8 535,229 - trpD DVU0467 0.17 -1.5 535,233 - trpD DVU0467 0.18 -0.9 535,239 + trpD DVU0467 0.18 -1.7 535,244 + trpD DVU0467 0.19 -2.0 535,244 + trpD DVU0467 0.19 -0.5 535,244 + trpD DVU0467 0.19 -1.6 535,244 + trpD DVU0467 0.19 -0.4 535,244 + trpD DVU0467 0.19 -1.4 535,252 - trpD DVU0467 0.19 -2.2 535,252 - trpD DVU0467 0.19 -0.9 535,252 - trpD DVU0467 0.19 +0.3 535,252 - trpD DVU0467 0.19 +0.3 535,257 - trpD DVU0467 0.20 -1.2 535,272 + trpD DVU0467 0.21 -0.6 535,447 - trpD DVU0467 0.39 -1.6 535,508 - trpD DVU0467 0.45 -0.7 535,546 + trpD DVU0467 0.49 -1.5 535,591 - trpD DVU0467 0.53 -1.7 535,634 - trpD DVU0467 0.58 -3.0 535,709 + trpD DVU0467 0.65 -0.8 535,756 - trpD DVU0467 0.70 -1.4 535,839 + trpD DVU0467 0.78 -0.6 535,841 + trpD DVU0467 0.78 -1.1 535,841 + trpD DVU0467 0.78 +2.5 535,849 - trpD DVU0467 0.79 -1.8 535,871 - trpD DVU0467 0.81 -1.6 535,897 + trpD DVU0467 0.84 -1.9 535,905 - trpD DVU0467 0.85 -1.3 535,920 - trpD DVU0467 0.86 -3.4 535,938 + trpD DVU0467 0.88 -0.9 535,942 - trpD DVU0467 0.88 -1.8 535,942 - trpD DVU0467 0.88 +0.4 535,955 + trpD DVU0467 0.90 +0.3 535,955 + trpD DVU0467 0.90 -1.8 535,963 - -2.1 535,983 + -0.4 535,985 + -2.6 535,985 + +1.3 535,985 + -1.8 536,095 - -1.5 536,158 + trpC DVU0468 0.14 -2.3 536,164 - trpC DVU0468 0.15 -2.8 536,166 - trpC DVU0468 0.15 -1.9 536,166 - trpC DVU0468 0.15 -3.1 536,179 + trpC DVU0468 0.17 -1.1 536,179 + trpC DVU0468 0.17 -1.7 536,179 + trpC DVU0468 0.17 -1.4 536,301 - trpC DVU0468 0.33 -0.6 536,303 + trpC DVU0468 0.33 -2.1 536,311 - trpC DVU0468 0.34 -1.3 536,318 + trpC DVU0468 0.35 -1.4 536,318 + trpC DVU0468 0.35 -2.9 536,369 - trpC DVU0468 0.41 -2.0 536,425 + trpC DVU0468 0.49 -1.7 536,461 + trpC DVU0468 0.53 -1.7 536,467 + trpC DVU0468 0.54 -1.4 536,506 - trpC DVU0468 0.59 -1.8 536,511 + trpC DVU0468 0.60 -1.7 536,519 - trpC DVU0468 0.61 -2.0 536,519 - trpC DVU0468 0.61 -1.9 536,547 - trpC DVU0468 0.64 -0.3 536,566 + trpC DVU0468 0.67 -0.9 536,611 + trpC DVU0468 0.73 -2.1 536,619 - trpC DVU0468 0.74 -2.1 536,669 + trpC DVU0468 0.80 -1.2 536,750 - -1.1 536,757 + -0.7 536,757 + +0.7 536,765 - -1.7 536,799 + -1.1 536,807 - -2.7 536,860 + -0.5 536,979 + trpF-1 DVU0469 0.13 -2.3 537,037 - trpF-1 DVU0469 0.20 -2.4 537,037 - trpF-1 DVU0469 0.20 -2.2 537,044 - trpF-1 DVU0469 0.20 -1.3 537,044 - trpF-1 DVU0469 0.20 -2.1 537,135 + trpF-1 DVU0469 0.31 -1.8 537,206 - trpF-1 DVU0469 0.39 -0.3 537,288 + trpF-1 DVU0469 0.49 -1.2 537,389 - trpF-1 DVU0469 0.61 -3.0 537,476 + trpF-1 DVU0469 0.71 +0.7 537,479 - trpF-1 DVU0469 0.71 -2.3 537,489 - trpF-1 DVU0469 0.72 -0.5 537,574 - trpF-1 DVU0469 0.82 -2.4 537,579 - trpF-1 DVU0469 0.83 -1.1 537,664 - -2.3 537,671 - -0.1 537,688 - -0.3 537,690 + +0.7 537,692 + -0.2
Or see this region's nucleotide sequence