Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_5859

Experiment: L-Leucine (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_5858 and Pf6N2E2_5859 overlap by 1 nucleotidesPf6N2E2_5859 and Pf6N2E2_5860 are separated by 489 nucleotides Pf6N2E2_5858 - Succinyl-CoA ligase [ADP-forming] beta chain (EC 6.2.1.5), at 6,677,616 to 6,678,782 _5858 Pf6N2E2_5859 - Succinyl-CoA ligase [ADP-forming] alpha chain (EC 6.2.1.5), at 6,678,782 to 6,679,666 _5859 Pf6N2E2_5860 - Branched-chain amino acid transport system carrier protein, at 6,680,156 to 6,681,469 _5860 Position (kb) 6678 6679 6680Strain fitness (log2 ratio) -1 0 1at 6679.837 kb on + strandat 6679.837 kb on + strandat 6679.838 kb on - strandat 6679.849 kb on + strandat 6679.935 kb on + strandat 6679.936 kb on - strandat 6679.944 kb on - strandat 6679.944 kb on - strandat 6679.944 kb on - strandat 6679.975 kb on - strandat 6679.975 kb on - strandat 6679.986 kb on + strandat 6680.064 kb on - strandat 6680.140 kb on + strandat 6680.420 kb on + strand, within Pf6N2E2_5860at 6680.525 kb on + strand, within Pf6N2E2_5860at 6680.526 kb on - strand, within Pf6N2E2_5860at 6680.537 kb on + strand, within Pf6N2E2_5860at 6680.537 kb on + strand, within Pf6N2E2_5860at 6680.537 kb on + strand, within Pf6N2E2_5860at 6680.537 kb on + strandat 6680.537 kb on + strand, within Pf6N2E2_5860

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Leucine (C)
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6,679,837 + -0.7
6,679,837 + +0.2
6,679,838 - +0.3
6,679,849 + +0.1
6,679,935 + +1.2
6,679,936 - -0.4
6,679,944 - +0.3
6,679,944 - +1.2
6,679,944 - +0.6
6,679,975 - +0.7
6,679,975 - +0.4
6,679,986 + -0.1
6,680,064 - -0.4
6,680,140 + -0.5
6,680,420 + Pf6N2E2_5860 0.20 +0.7
6,680,525 + Pf6N2E2_5860 0.28 -0.1
6,680,526 - Pf6N2E2_5860 0.28 +0.2
6,680,537 + Pf6N2E2_5860 0.29 +0.8
6,680,537 + Pf6N2E2_5860 0.29 -0.5
6,680,537 + Pf6N2E2_5860 0.29 -0.7
6,680,537 + +0.1
6,680,537 + Pf6N2E2_5860 0.29 +0.9

Or see this region's nucleotide sequence