Experiment: L-Leucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5475 and Pf6N2E2_5476 overlap by 4 nucleotides Pf6N2E2_5476 and Pf6N2E2_5477 are separated by 215 nucleotides
Pf6N2E2_5475 - Acetolactate synthase, large subunit (EC 2.2.1.6), at 6,291,769 to 6,293,406
_5475
Pf6N2E2_5476 - Aldehyde dehydrogenase (EC 1.2.1.3), at 6,293,403 to 6,294,851
_5476
Pf6N2E2_5477 - ABC transporter, periplasmic spermidine putrescine-binding protein PotD (TC 3.A.1.11.1), at 6,295,067 to 6,296,170
_5477
Position (kb)
6293
6294
6295 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 6292.603 kb on + strand, within Pf6N2E2_5475 at 6292.688 kb on - strand, within Pf6N2E2_5475 at 6292.930 kb on + strand, within Pf6N2E2_5475 at 6292.931 kb on - strand, within Pf6N2E2_5475 at 6292.931 kb on - strand, within Pf6N2E2_5475 at 6293.172 kb on + strand, within Pf6N2E2_5475 at 6293.183 kb on - strand, within Pf6N2E2_5475 at 6293.200 kb on + strand, within Pf6N2E2_5475 at 6293.200 kb on + strand, within Pf6N2E2_5475 at 6293.200 kb on + strand, within Pf6N2E2_5475 at 6293.200 kb on + strand, within Pf6N2E2_5475 at 6293.200 kb on + strand, within Pf6N2E2_5475 at 6293.200 kb on + strand, within Pf6N2E2_5475 at 6293.201 kb on - strand, within Pf6N2E2_5475 at 6293.201 kb on - strand, within Pf6N2E2_5475 at 6293.201 kb on - strand, within Pf6N2E2_5475 at 6293.242 kb on + strand, within Pf6N2E2_5475 at 6293.242 kb on + strand, within Pf6N2E2_5475 at 6293.242 kb on + strand, within Pf6N2E2_5475 at 6293.242 kb on + strand, within Pf6N2E2_5475 at 6293.242 kb on + strand, within Pf6N2E2_5475 at 6293.242 kb on + strand, within Pf6N2E2_5475 at 6293.242 kb on + strand, within Pf6N2E2_5475 at 6293.242 kb on + strand, within Pf6N2E2_5475 at 6293.242 kb on + strand, within Pf6N2E2_5475 at 6293.242 kb on + strand, within Pf6N2E2_5475 at 6293.243 kb on - strand at 6293.243 kb on - strand at 6293.243 kb on - strand at 6293.243 kb on - strand at 6293.243 kb on - strand at 6293.243 kb on - strand at 6293.243 kb on - strand at 6293.243 kb on - strand at 6293.433 kb on + strand at 6293.433 kb on + strand at 6293.433 kb on + strand at 6293.433 kb on + strand at 6293.433 kb on + strand at 6293.433 kb on + strand at 6293.433 kb on + strand at 6293.433 kb on + strand at 6293.434 kb on - strand at 6293.434 kb on - strand at 6293.434 kb on - strand at 6293.434 kb on - strand at 6293.434 kb on - strand at 6293.739 kb on + strand, within Pf6N2E2_5476 at 6293.740 kb on - strand, within Pf6N2E2_5476 at 6293.742 kb on + strand, within Pf6N2E2_5476 at 6294.406 kb on - strand, within Pf6N2E2_5476 at 6294.765 kb on + strand at 6294.766 kb on - strand at 6294.766 kb on - strand at 6295.041 kb on + strand at 6295.042 kb on - strand at 6295.042 kb on - strand at 6295.042 kb on - strand at 6295.045 kb on + strand at 6295.045 kb on + strand at 6295.045 kb on + strand at 6295.046 kb on - strand at 6295.053 kb on + strand at 6295.054 kb on - strand at 6295.306 kb on + strand, within Pf6N2E2_5477 at 6295.356 kb on - strand, within Pf6N2E2_5477 at 6295.356 kb on - strand, within Pf6N2E2_5477 at 6295.363 kb on + strand, within Pf6N2E2_5477 at 6295.363 kb on + strand, within Pf6N2E2_5477 at 6295.364 kb on - strand, within Pf6N2E2_5477 at 6295.364 kb on - strand, within Pf6N2E2_5477 at 6295.364 kb on - strand, within Pf6N2E2_5477 at 6295.499 kb on + strand, within Pf6N2E2_5477 at 6295.526 kb on + strand, within Pf6N2E2_5477 at 6295.537 kb on + strand, within Pf6N2E2_5477 at 6295.562 kb on + strand, within Pf6N2E2_5477 at 6295.562 kb on + strand, within Pf6N2E2_5477 at 6295.563 kb on - strand, within Pf6N2E2_5477 at 6295.799 kb on + strand, within Pf6N2E2_5477 at 6295.800 kb on - strand, within Pf6N2E2_5477 at 6295.800 kb on - strand, within Pf6N2E2_5477 at 6295.841 kb on + strand, within Pf6N2E2_5477 at 6295.842 kb on - strand, within Pf6N2E2_5477
Per-strain Table
Position Strand Gene LocusTag Fraction L-Leucine (C) remove 6,292,603 + Pf6N2E2_5475 0.51 -1.2 6,292,688 - Pf6N2E2_5475 0.56 -0.4 6,292,930 + Pf6N2E2_5475 0.71 +0.8 6,292,931 - Pf6N2E2_5475 0.71 +0.2 6,292,931 - Pf6N2E2_5475 0.71 +0.5 6,293,172 + Pf6N2E2_5475 0.86 -0.6 6,293,183 - Pf6N2E2_5475 0.86 +0.3 6,293,200 + Pf6N2E2_5475 0.87 +0.5 6,293,200 + Pf6N2E2_5475 0.87 -2.0 6,293,200 + Pf6N2E2_5475 0.87 +0.6 6,293,200 + Pf6N2E2_5475 0.87 -1.4 6,293,200 + Pf6N2E2_5475 0.87 -2.1 6,293,200 + Pf6N2E2_5475 0.87 -0.9 6,293,201 - Pf6N2E2_5475 0.87 +0.1 6,293,201 - Pf6N2E2_5475 0.87 -1.0 6,293,201 - Pf6N2E2_5475 0.87 +0.2 6,293,242 + Pf6N2E2_5475 0.90 -0.7 6,293,242 + Pf6N2E2_5475 0.90 +0.1 6,293,242 + Pf6N2E2_5475 0.90 +0.6 6,293,242 + Pf6N2E2_5475 0.90 -0.3 6,293,242 + Pf6N2E2_5475 0.90 -0.5 6,293,242 + Pf6N2E2_5475 0.90 -0.8 6,293,242 + Pf6N2E2_5475 0.90 +0.4 6,293,242 + Pf6N2E2_5475 0.90 +0.0 6,293,242 + Pf6N2E2_5475 0.90 +0.4 6,293,242 + Pf6N2E2_5475 0.90 -0.1 6,293,243 - +0.3 6,293,243 - -1.0 6,293,243 - -0.6 6,293,243 - -0.6 6,293,243 - -2.9 6,293,243 - +1.9 6,293,243 - -0.8 6,293,243 - +0.5 6,293,433 + -0.3 6,293,433 + +0.2 6,293,433 + +0.3 6,293,433 + +0.2 6,293,433 + -2.4 6,293,433 + +0.8 6,293,433 + -1.4 6,293,433 + +1.1 6,293,434 - +0.2 6,293,434 - -0.5 6,293,434 - +1.0 6,293,434 - -1.8 6,293,434 - -1.5 6,293,739 + Pf6N2E2_5476 0.23 -1.0 6,293,740 - Pf6N2E2_5476 0.23 -2.3 6,293,742 + Pf6N2E2_5476 0.23 +0.2 6,294,406 - Pf6N2E2_5476 0.69 -0.8 6,294,765 + +0.4 6,294,766 - +0.7 6,294,766 - +0.4 6,295,041 + +0.3 6,295,042 - +0.1 6,295,042 - +0.1 6,295,042 - +0.2 6,295,045 + +0.3 6,295,045 + +0.8 6,295,045 + -0.1 6,295,046 - +0.2 6,295,053 + -0.5 6,295,054 - +0.5 6,295,306 + Pf6N2E2_5477 0.22 +0.7 6,295,356 - Pf6N2E2_5477 0.26 +1.0 6,295,356 - Pf6N2E2_5477 0.26 -1.0 6,295,363 + Pf6N2E2_5477 0.27 +1.3 6,295,363 + Pf6N2E2_5477 0.27 -0.0 6,295,364 - Pf6N2E2_5477 0.27 +0.3 6,295,364 - Pf6N2E2_5477 0.27 -0.2 6,295,364 - Pf6N2E2_5477 0.27 -0.0 6,295,499 + Pf6N2E2_5477 0.39 -0.3 6,295,526 + Pf6N2E2_5477 0.42 +0.2 6,295,537 + Pf6N2E2_5477 0.43 +1.4 6,295,562 + Pf6N2E2_5477 0.45 +0.7 6,295,562 + Pf6N2E2_5477 0.45 +0.4 6,295,563 - Pf6N2E2_5477 0.45 -0.3 6,295,799 + Pf6N2E2_5477 0.66 +0.7 6,295,800 - Pf6N2E2_5477 0.66 +0.6 6,295,800 - Pf6N2E2_5477 0.66 -0.1 6,295,841 + Pf6N2E2_5477 0.70 +0.2 6,295,842 - Pf6N2E2_5477 0.70 -0.4
Or see this region's nucleotide sequence