Experiment: L-Leucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_480 and Pf6N2E2_481 are separated by 1 nucleotides Pf6N2E2_481 and Pf6N2E2_482 are separated by 192 nucleotides Pf6N2E2_482 and Pf6N2E2_483 are separated by 151 nucleotides
Pf6N2E2_480 - 3-methyl-2-oxobutanoate dehydrogenase (2-methylpropanoyl-transferring) (EC 1.2.4.4) (from data) , at 546,161 to 547,219
_480
Pf6N2E2_481 - branched-chain alpha-ketoacid dehydrogenase, E1 component alpha subunit (EC 1.2.4.4) (from data) , at 547,221 to 548,456
_481
Pf6N2E2_482 - Transcriptional regulator BkdR of isoleucine and valine catabolism operon, at 548,649 to 549,137
_482
Pf6N2E2_483 - FIG146518: Zn-dependent hydrolases, including glyoxylases, at 549,289 to 550,152
_483
Position (kb)
547
548
549 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 546.483 kb on + strand, within Pf6N2E2_480 at 546.483 kb on + strand, within Pf6N2E2_480 at 546.483 kb on + strand, within Pf6N2E2_480 at 546.483 kb on + strand, within Pf6N2E2_480 at 546.483 kb on + strand, within Pf6N2E2_480 at 546.484 kb on - strand, within Pf6N2E2_480 at 546.484 kb on - strand, within Pf6N2E2_480 at 546.484 kb on - strand, within Pf6N2E2_480 at 546.484 kb on - strand, within Pf6N2E2_480 at 546.484 kb on - strand, within Pf6N2E2_480 at 546.484 kb on - strand, within Pf6N2E2_480 at 546.484 kb on - strand, within Pf6N2E2_480 at 546.484 kb on - strand, within Pf6N2E2_480 at 546.484 kb on - strand, within Pf6N2E2_480 at 546.484 kb on - strand, within Pf6N2E2_480 at 546.484 kb on - strand, within Pf6N2E2_480 at 546.498 kb on + strand, within Pf6N2E2_480 at 546.498 kb on + strand, within Pf6N2E2_480 at 546.498 kb on + strand, within Pf6N2E2_480 at 546.499 kb on - strand, within Pf6N2E2_480 at 546.499 kb on - strand, within Pf6N2E2_480 at 546.499 kb on - strand at 546.561 kb on + strand, within Pf6N2E2_480 at 546.561 kb on + strand, within Pf6N2E2_480 at 546.562 kb on - strand, within Pf6N2E2_480 at 546.562 kb on - strand, within Pf6N2E2_480 at 546.562 kb on - strand, within Pf6N2E2_480 at 546.565 kb on - strand, within Pf6N2E2_480 at 546.645 kb on + strand, within Pf6N2E2_480 at 546.645 kb on + strand, within Pf6N2E2_480 at 546.645 kb on + strand, within Pf6N2E2_480 at 546.646 kb on - strand, within Pf6N2E2_480 at 546.646 kb on - strand, within Pf6N2E2_480 at 546.721 kb on - strand, within Pf6N2E2_480 at 546.804 kb on + strand, within Pf6N2E2_480 at 546.804 kb on + strand, within Pf6N2E2_480 at 546.804 kb on + strand, within Pf6N2E2_480 at 546.804 kb on + strand, within Pf6N2E2_480 at 546.804 kb on + strand, within Pf6N2E2_480 at 546.805 kb on - strand, within Pf6N2E2_480 at 546.805 kb on - strand, within Pf6N2E2_480 at 546.913 kb on - strand, within Pf6N2E2_480 at 546.918 kb on + strand, within Pf6N2E2_480 at 546.918 kb on + strand, within Pf6N2E2_480 at 546.918 kb on + strand, within Pf6N2E2_480 at 546.918 kb on + strand, within Pf6N2E2_480 at 546.919 kb on - strand, within Pf6N2E2_480 at 546.919 kb on - strand, within Pf6N2E2_480 at 546.919 kb on - strand, within Pf6N2E2_480 at 546.919 kb on - strand, within Pf6N2E2_480 at 547.032 kb on + strand, within Pf6N2E2_480 at 547.033 kb on - strand, within Pf6N2E2_480 at 547.033 kb on - strand, within Pf6N2E2_480 at 547.033 kb on - strand, within Pf6N2E2_480 at 547.077 kb on + strand, within Pf6N2E2_480 at 547.078 kb on - strand at 547.198 kb on + strand at 547.199 kb on - strand at 547.199 kb on - strand at 547.274 kb on - strand at 547.274 kb on - strand at 547.427 kb on + strand, within Pf6N2E2_481 at 547.427 kb on + strand, within Pf6N2E2_481 at 547.427 kb on + strand, within Pf6N2E2_481 at 547.427 kb on + strand, within Pf6N2E2_481 at 547.428 kb on - strand, within Pf6N2E2_481 at 547.428 kb on - strand, within Pf6N2E2_481 at 547.945 kb on + strand, within Pf6N2E2_481 at 547.945 kb on + strand, within Pf6N2E2_481 at 547.945 kb on + strand, within Pf6N2E2_481 at 547.945 kb on + strand, within Pf6N2E2_481 at 547.946 kb on - strand, within Pf6N2E2_481 at 547.946 kb on - strand, within Pf6N2E2_481 at 547.946 kb on - strand, within Pf6N2E2_481 at 547.946 kb on - strand, within Pf6N2E2_481 at 547.957 kb on - strand, within Pf6N2E2_481 at 548.137 kb on + strand, within Pf6N2E2_481 at 548.138 kb on - strand, within Pf6N2E2_481 at 548.185 kb on + strand, within Pf6N2E2_481 at 548.185 kb on + strand at 548.186 kb on - strand, within Pf6N2E2_481 at 548.345 kb on + strand at 548.377 kb on + strand at 548.378 kb on - strand at 548.464 kb on + strand at 548.465 kb on - strand at 548.465 kb on - strand at 548.465 kb on - strand at 548.489 kb on + strand at 548.502 kb on - strand at 548.537 kb on + strand at 548.538 kb on - strand at 548.548 kb on + strand at 548.549 kb on - strand at 548.549 kb on - strand at 548.829 kb on - strand, within Pf6N2E2_482 at 548.943 kb on + strand, within Pf6N2E2_482 at 548.943 kb on + strand, within Pf6N2E2_482 at 549.290 kb on + strand at 549.291 kb on - strand at 549.291 kb on - strand at 549.291 kb on - strand at 549.317 kb on + strand at 549.317 kb on + strand at 549.318 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Leucine (C) remove 546,483 + Pf6N2E2_480 0.30 -3.7 546,483 + Pf6N2E2_480 0.30 -3.5 546,483 + Pf6N2E2_480 0.30 -3.2 546,483 + Pf6N2E2_480 0.30 -2.5 546,483 + Pf6N2E2_480 0.30 -3.3 546,484 - Pf6N2E2_480 0.31 -3.3 546,484 - Pf6N2E2_480 0.31 -2.9 546,484 - Pf6N2E2_480 0.31 -3.5 546,484 - Pf6N2E2_480 0.31 -3.9 546,484 - Pf6N2E2_480 0.31 -3.3 546,484 - Pf6N2E2_480 0.31 -2.7 546,484 - Pf6N2E2_480 0.31 -2.9 546,484 - Pf6N2E2_480 0.31 -2.8 546,484 - Pf6N2E2_480 0.31 -3.9 546,484 - Pf6N2E2_480 0.31 -2.5 546,484 - Pf6N2E2_480 0.31 -2.9 546,498 + Pf6N2E2_480 0.32 -2.5 546,498 + Pf6N2E2_480 0.32 -3.9 546,498 + Pf6N2E2_480 0.32 -3.0 546,499 - Pf6N2E2_480 0.32 -2.5 546,499 - Pf6N2E2_480 0.32 -3.0 546,499 - -0.9 546,561 + Pf6N2E2_480 0.38 -3.7 546,561 + Pf6N2E2_480 0.38 -3.0 546,562 - Pf6N2E2_480 0.38 -3.2 546,562 - Pf6N2E2_480 0.38 -3.1 546,562 - Pf6N2E2_480 0.38 -3.6 546,565 - Pf6N2E2_480 0.38 -3.1 546,645 + Pf6N2E2_480 0.46 -4.0 546,645 + Pf6N2E2_480 0.46 -2.5 546,645 + Pf6N2E2_480 0.46 -2.4 546,646 - Pf6N2E2_480 0.46 -1.6 546,646 - Pf6N2E2_480 0.46 -2.5 546,721 - Pf6N2E2_480 0.53 -1.3 546,804 + Pf6N2E2_480 0.61 -1.9 546,804 + Pf6N2E2_480 0.61 -2.9 546,804 + Pf6N2E2_480 0.61 -1.3 546,804 + Pf6N2E2_480 0.61 -3.0 546,804 + Pf6N2E2_480 0.61 -1.6 546,805 - Pf6N2E2_480 0.61 -3.8 546,805 - Pf6N2E2_480 0.61 -1.5 546,913 - Pf6N2E2_480 0.71 -1.9 546,918 + Pf6N2E2_480 0.71 -2.5 546,918 + Pf6N2E2_480 0.71 -1.9 546,918 + Pf6N2E2_480 0.71 -3.1 546,918 + Pf6N2E2_480 0.71 -1.6 546,919 - Pf6N2E2_480 0.72 -3.9 546,919 - Pf6N2E2_480 0.72 -1.5 546,919 - Pf6N2E2_480 0.72 -2.6 546,919 - Pf6N2E2_480 0.72 -3.6 547,032 + Pf6N2E2_480 0.82 -1.9 547,033 - Pf6N2E2_480 0.82 -2.5 547,033 - Pf6N2E2_480 0.82 -2.5 547,033 - Pf6N2E2_480 0.82 -3.5 547,077 + Pf6N2E2_480 0.86 -3.3 547,078 - -1.3 547,198 + -1.6 547,199 - -2.3 547,199 - -2.0 547,274 - -2.5 547,274 - -2.6 547,427 + Pf6N2E2_481 0.17 -3.5 547,427 + Pf6N2E2_481 0.17 -3.9 547,427 + Pf6N2E2_481 0.17 -2.5 547,427 + Pf6N2E2_481 0.17 -2.9 547,428 - Pf6N2E2_481 0.17 -1.6 547,428 - Pf6N2E2_481 0.17 -1.6 547,945 + Pf6N2E2_481 0.59 -1.6 547,945 + Pf6N2E2_481 0.59 -1.9 547,945 + Pf6N2E2_481 0.59 -2.6 547,945 + Pf6N2E2_481 0.59 -0.9 547,946 - Pf6N2E2_481 0.59 -3.9 547,946 - Pf6N2E2_481 0.59 -4.0 547,946 - Pf6N2E2_481 0.59 -2.3 547,946 - Pf6N2E2_481 0.59 -3.6 547,957 - Pf6N2E2_481 0.60 -2.5 548,137 + Pf6N2E2_481 0.74 -2.0 548,138 - Pf6N2E2_481 0.74 -0.5 548,185 + Pf6N2E2_481 0.78 -0.9 548,185 + -1.6 548,186 - Pf6N2E2_481 0.78 -3.5 548,345 + -3.0 548,377 + -4.0 548,378 - -3.5 548,464 + -2.3 548,465 - -2.7 548,465 - -2.7 548,465 - -3.4 548,489 + -3.5 548,502 - -1.1 548,537 + -3.0 548,538 - -2.7 548,548 + -1.1 548,549 - -0.9 548,549 - +0.1 548,829 - Pf6N2E2_482 0.37 -2.7 548,943 + Pf6N2E2_482 0.60 -3.5 548,943 + Pf6N2E2_482 0.60 -5.0 549,290 + -1.3 549,291 - +0.4 549,291 - -0.0 549,291 - +0.1 549,317 + +1.8 549,317 + +0.1 549,318 - +0.5
Or see this region's nucleotide sequence