Experiment: L-Leucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_478 and Pf6N2E2_479 are separated by 3 nucleotides Pf6N2E2_479 and Pf6N2E2_480 are separated by 0 nucleotides
Pf6N2E2_478 - Dihydrolipoyl dehydrogenase (EC 1.8.1.4) (from data) , at 543,500 to 544,885
_478
Pf6N2E2_479 - Dihydrolipoyllysine-residue (2-methylpropanoyl)transferase (EC 2.3.1.168) (from data) , at 544,889 to 546,160
_479
Pf6N2E2_480 - 3-methyl-2-oxobutanoate dehydrogenase (2-methylpropanoyl-transferring) (EC 1.2.4.4) (from data) , at 546,161 to 547,219
_480
Position (kb)
544
545
546
547 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 544.195 kb on - strand, within Pf6N2E2_478 at 544.195 kb on - strand, within Pf6N2E2_478 at 544.341 kb on + strand, within Pf6N2E2_478 at 544.342 kb on - strand, within Pf6N2E2_478 at 544.830 kb on + strand at 544.891 kb on - strand at 545.075 kb on + strand, within Pf6N2E2_479 at 545.497 kb on - strand, within Pf6N2E2_479 at 545.599 kb on - strand, within Pf6N2E2_479 at 545.599 kb on - strand, within Pf6N2E2_479 at 545.643 kb on + strand, within Pf6N2E2_479 at 545.644 kb on - strand, within Pf6N2E2_479 at 545.644 kb on - strand, within Pf6N2E2_479 at 546.053 kb on + strand at 546.053 kb on + strand at 546.146 kb on - strand at 546.216 kb on + strand at 546.216 kb on + strand at 546.217 kb on - strand at 546.483 kb on + strand, within Pf6N2E2_480 at 546.483 kb on + strand, within Pf6N2E2_480 at 546.483 kb on + strand, within Pf6N2E2_480 at 546.483 kb on + strand, within Pf6N2E2_480 at 546.483 kb on + strand, within Pf6N2E2_480 at 546.484 kb on - strand, within Pf6N2E2_480 at 546.484 kb on - strand, within Pf6N2E2_480 at 546.484 kb on - strand, within Pf6N2E2_480 at 546.484 kb on - strand, within Pf6N2E2_480 at 546.484 kb on - strand, within Pf6N2E2_480 at 546.484 kb on - strand, within Pf6N2E2_480 at 546.484 kb on - strand, within Pf6N2E2_480 at 546.484 kb on - strand, within Pf6N2E2_480 at 546.484 kb on - strand, within Pf6N2E2_480 at 546.484 kb on - strand, within Pf6N2E2_480 at 546.484 kb on - strand, within Pf6N2E2_480 at 546.498 kb on + strand, within Pf6N2E2_480 at 546.498 kb on + strand, within Pf6N2E2_480 at 546.498 kb on + strand, within Pf6N2E2_480 at 546.499 kb on - strand, within Pf6N2E2_480 at 546.499 kb on - strand, within Pf6N2E2_480 at 546.499 kb on - strand at 546.561 kb on + strand, within Pf6N2E2_480 at 546.561 kb on + strand, within Pf6N2E2_480 at 546.562 kb on - strand, within Pf6N2E2_480 at 546.562 kb on - strand, within Pf6N2E2_480 at 546.562 kb on - strand, within Pf6N2E2_480 at 546.565 kb on - strand, within Pf6N2E2_480 at 546.645 kb on + strand, within Pf6N2E2_480 at 546.645 kb on + strand, within Pf6N2E2_480 at 546.645 kb on + strand, within Pf6N2E2_480 at 546.646 kb on - strand, within Pf6N2E2_480 at 546.646 kb on - strand, within Pf6N2E2_480 at 546.721 kb on - strand, within Pf6N2E2_480 at 546.804 kb on + strand, within Pf6N2E2_480 at 546.804 kb on + strand, within Pf6N2E2_480 at 546.804 kb on + strand, within Pf6N2E2_480 at 546.804 kb on + strand, within Pf6N2E2_480 at 546.804 kb on + strand, within Pf6N2E2_480 at 546.805 kb on - strand, within Pf6N2E2_480 at 546.805 kb on - strand, within Pf6N2E2_480 at 546.913 kb on - strand, within Pf6N2E2_480 at 546.918 kb on + strand, within Pf6N2E2_480 at 546.918 kb on + strand, within Pf6N2E2_480 at 546.918 kb on + strand, within Pf6N2E2_480 at 546.918 kb on + strand, within Pf6N2E2_480 at 546.919 kb on - strand, within Pf6N2E2_480 at 546.919 kb on - strand, within Pf6N2E2_480 at 546.919 kb on - strand, within Pf6N2E2_480 at 546.919 kb on - strand, within Pf6N2E2_480 at 547.032 kb on + strand, within Pf6N2E2_480 at 547.033 kb on - strand, within Pf6N2E2_480 at 547.033 kb on - strand, within Pf6N2E2_480 at 547.033 kb on - strand, within Pf6N2E2_480 at 547.077 kb on + strand, within Pf6N2E2_480 at 547.078 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Leucine (C) remove 544,195 - Pf6N2E2_478 0.50 -3.6 544,195 - Pf6N2E2_478 0.50 -2.5 544,341 + Pf6N2E2_478 0.61 -1.6 544,342 - Pf6N2E2_478 0.61 -2.2 544,830 + -2.7 544,891 - -3.8 545,075 + Pf6N2E2_479 0.15 -3.4 545,497 - Pf6N2E2_479 0.48 -3.3 545,599 - Pf6N2E2_479 0.56 -2.6 545,599 - Pf6N2E2_479 0.56 -3.5 545,643 + Pf6N2E2_479 0.59 -3.5 545,644 - Pf6N2E2_479 0.59 -4.1 545,644 - Pf6N2E2_479 0.59 -4.1 546,053 + -4.6 546,053 + -3.7 546,146 - -1.7 546,216 + -5.2 546,216 + -0.6 546,217 - -3.3 546,483 + Pf6N2E2_480 0.30 -3.7 546,483 + Pf6N2E2_480 0.30 -3.5 546,483 + Pf6N2E2_480 0.30 -3.2 546,483 + Pf6N2E2_480 0.30 -2.5 546,483 + Pf6N2E2_480 0.30 -3.3 546,484 - Pf6N2E2_480 0.31 -3.3 546,484 - Pf6N2E2_480 0.31 -2.9 546,484 - Pf6N2E2_480 0.31 -3.5 546,484 - Pf6N2E2_480 0.31 -3.9 546,484 - Pf6N2E2_480 0.31 -3.3 546,484 - Pf6N2E2_480 0.31 -2.7 546,484 - Pf6N2E2_480 0.31 -2.9 546,484 - Pf6N2E2_480 0.31 -2.8 546,484 - Pf6N2E2_480 0.31 -3.9 546,484 - Pf6N2E2_480 0.31 -2.5 546,484 - Pf6N2E2_480 0.31 -2.9 546,498 + Pf6N2E2_480 0.32 -2.5 546,498 + Pf6N2E2_480 0.32 -3.9 546,498 + Pf6N2E2_480 0.32 -3.0 546,499 - Pf6N2E2_480 0.32 -2.5 546,499 - Pf6N2E2_480 0.32 -3.0 546,499 - -0.9 546,561 + Pf6N2E2_480 0.38 -3.7 546,561 + Pf6N2E2_480 0.38 -3.0 546,562 - Pf6N2E2_480 0.38 -3.2 546,562 - Pf6N2E2_480 0.38 -3.1 546,562 - Pf6N2E2_480 0.38 -3.6 546,565 - Pf6N2E2_480 0.38 -3.1 546,645 + Pf6N2E2_480 0.46 -4.0 546,645 + Pf6N2E2_480 0.46 -2.5 546,645 + Pf6N2E2_480 0.46 -2.4 546,646 - Pf6N2E2_480 0.46 -1.6 546,646 - Pf6N2E2_480 0.46 -2.5 546,721 - Pf6N2E2_480 0.53 -1.3 546,804 + Pf6N2E2_480 0.61 -1.9 546,804 + Pf6N2E2_480 0.61 -2.9 546,804 + Pf6N2E2_480 0.61 -1.3 546,804 + Pf6N2E2_480 0.61 -3.0 546,804 + Pf6N2E2_480 0.61 -1.6 546,805 - Pf6N2E2_480 0.61 -3.8 546,805 - Pf6N2E2_480 0.61 -1.5 546,913 - Pf6N2E2_480 0.71 -1.9 546,918 + Pf6N2E2_480 0.71 -2.5 546,918 + Pf6N2E2_480 0.71 -1.9 546,918 + Pf6N2E2_480 0.71 -3.1 546,918 + Pf6N2E2_480 0.71 -1.6 546,919 - Pf6N2E2_480 0.72 -3.9 546,919 - Pf6N2E2_480 0.72 -1.5 546,919 - Pf6N2E2_480 0.72 -2.6 546,919 - Pf6N2E2_480 0.72 -3.6 547,032 + Pf6N2E2_480 0.82 -1.9 547,033 - Pf6N2E2_480 0.82 -2.5 547,033 - Pf6N2E2_480 0.82 -2.5 547,033 - Pf6N2E2_480 0.82 -3.5 547,077 + Pf6N2E2_480 0.86 -3.3 547,078 - -1.3
Or see this region's nucleotide sequence