Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_479

Experiment: L-Leucine (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_478 and Pf6N2E2_479 are separated by 3 nucleotidesPf6N2E2_479 and Pf6N2E2_480 are separated by 0 nucleotides Pf6N2E2_478 - Dihydrolipoyl dehydrogenase (EC 1.8.1.4) (from data), at 543,500 to 544,885 _478 Pf6N2E2_479 - Dihydrolipoyllysine-residue (2-methylpropanoyl)transferase (EC 2.3.1.168) (from data), at 544,889 to 546,160 _479 Pf6N2E2_480 - 3-methyl-2-oxobutanoate dehydrogenase (2-methylpropanoyl-transferring) (EC 1.2.4.4) (from data), at 546,161 to 547,219 _480 Position (kb) 544 545 546 547Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1at 544.195 kb on - strand, within Pf6N2E2_478at 544.195 kb on - strand, within Pf6N2E2_478at 544.341 kb on + strand, within Pf6N2E2_478at 544.342 kb on - strand, within Pf6N2E2_478at 544.830 kb on + strandat 544.891 kb on - strandat 545.075 kb on + strand, within Pf6N2E2_479at 545.497 kb on - strand, within Pf6N2E2_479at 545.599 kb on - strand, within Pf6N2E2_479at 545.599 kb on - strand, within Pf6N2E2_479at 545.643 kb on + strand, within Pf6N2E2_479at 545.644 kb on - strand, within Pf6N2E2_479at 545.644 kb on - strand, within Pf6N2E2_479at 546.053 kb on + strandat 546.053 kb on + strandat 546.146 kb on - strandat 546.216 kb on + strandat 546.216 kb on + strandat 546.217 kb on - strandat 546.483 kb on + strand, within Pf6N2E2_480at 546.483 kb on + strand, within Pf6N2E2_480at 546.483 kb on + strand, within Pf6N2E2_480at 546.483 kb on + strand, within Pf6N2E2_480at 546.483 kb on + strand, within Pf6N2E2_480at 546.484 kb on - strand, within Pf6N2E2_480at 546.484 kb on - strand, within Pf6N2E2_480at 546.484 kb on - strand, within Pf6N2E2_480at 546.484 kb on - strand, within Pf6N2E2_480at 546.484 kb on - strand, within Pf6N2E2_480at 546.484 kb on - strand, within Pf6N2E2_480at 546.484 kb on - strand, within Pf6N2E2_480at 546.484 kb on - strand, within Pf6N2E2_480at 546.484 kb on - strand, within Pf6N2E2_480at 546.484 kb on - strand, within Pf6N2E2_480at 546.484 kb on - strand, within Pf6N2E2_480at 546.498 kb on + strand, within Pf6N2E2_480at 546.498 kb on + strand, within Pf6N2E2_480at 546.498 kb on + strand, within Pf6N2E2_480at 546.499 kb on - strand, within Pf6N2E2_480at 546.499 kb on - strand, within Pf6N2E2_480at 546.499 kb on - strandat 546.561 kb on + strand, within Pf6N2E2_480at 546.561 kb on + strand, within Pf6N2E2_480at 546.562 kb on - strand, within Pf6N2E2_480at 546.562 kb on - strand, within Pf6N2E2_480at 546.562 kb on - strand, within Pf6N2E2_480at 546.565 kb on - strand, within Pf6N2E2_480at 546.645 kb on + strand, within Pf6N2E2_480at 546.645 kb on + strand, within Pf6N2E2_480at 546.645 kb on + strand, within Pf6N2E2_480at 546.646 kb on - strand, within Pf6N2E2_480at 546.646 kb on - strand, within Pf6N2E2_480at 546.721 kb on - strand, within Pf6N2E2_480at 546.804 kb on + strand, within Pf6N2E2_480at 546.804 kb on + strand, within Pf6N2E2_480at 546.804 kb on + strand, within Pf6N2E2_480at 546.804 kb on + strand, within Pf6N2E2_480at 546.804 kb on + strand, within Pf6N2E2_480at 546.805 kb on - strand, within Pf6N2E2_480at 546.805 kb on - strand, within Pf6N2E2_480at 546.913 kb on - strand, within Pf6N2E2_480at 546.918 kb on + strand, within Pf6N2E2_480at 546.918 kb on + strand, within Pf6N2E2_480at 546.918 kb on + strand, within Pf6N2E2_480at 546.918 kb on + strand, within Pf6N2E2_480at 546.919 kb on - strand, within Pf6N2E2_480at 546.919 kb on - strand, within Pf6N2E2_480at 546.919 kb on - strand, within Pf6N2E2_480at 546.919 kb on - strand, within Pf6N2E2_480at 547.032 kb on + strand, within Pf6N2E2_480at 547.033 kb on - strand, within Pf6N2E2_480at 547.033 kb on - strand, within Pf6N2E2_480at 547.033 kb on - strand, within Pf6N2E2_480at 547.077 kb on + strand, within Pf6N2E2_480at 547.078 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Leucine (C)
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544,195 - Pf6N2E2_478 0.50 -3.6
544,195 - Pf6N2E2_478 0.50 -2.5
544,341 + Pf6N2E2_478 0.61 -1.6
544,342 - Pf6N2E2_478 0.61 -2.2
544,830 + -2.7
544,891 - -3.8
545,075 + Pf6N2E2_479 0.15 -3.4
545,497 - Pf6N2E2_479 0.48 -3.3
545,599 - Pf6N2E2_479 0.56 -2.6
545,599 - Pf6N2E2_479 0.56 -3.5
545,643 + Pf6N2E2_479 0.59 -3.5
545,644 - Pf6N2E2_479 0.59 -4.1
545,644 - Pf6N2E2_479 0.59 -4.1
546,053 + -4.6
546,053 + -3.7
546,146 - -1.7
546,216 + -5.2
546,216 + -0.6
546,217 - -3.3
546,483 + Pf6N2E2_480 0.30 -3.7
546,483 + Pf6N2E2_480 0.30 -3.5
546,483 + Pf6N2E2_480 0.30 -3.2
546,483 + Pf6N2E2_480 0.30 -2.5
546,483 + Pf6N2E2_480 0.30 -3.3
546,484 - Pf6N2E2_480 0.31 -3.3
546,484 - Pf6N2E2_480 0.31 -2.9
546,484 - Pf6N2E2_480 0.31 -3.5
546,484 - Pf6N2E2_480 0.31 -3.9
546,484 - Pf6N2E2_480 0.31 -3.3
546,484 - Pf6N2E2_480 0.31 -2.7
546,484 - Pf6N2E2_480 0.31 -2.9
546,484 - Pf6N2E2_480 0.31 -2.8
546,484 - Pf6N2E2_480 0.31 -3.9
546,484 - Pf6N2E2_480 0.31 -2.5
546,484 - Pf6N2E2_480 0.31 -2.9
546,498 + Pf6N2E2_480 0.32 -2.5
546,498 + Pf6N2E2_480 0.32 -3.9
546,498 + Pf6N2E2_480 0.32 -3.0
546,499 - Pf6N2E2_480 0.32 -2.5
546,499 - Pf6N2E2_480 0.32 -3.0
546,499 - -0.9
546,561 + Pf6N2E2_480 0.38 -3.7
546,561 + Pf6N2E2_480 0.38 -3.0
546,562 - Pf6N2E2_480 0.38 -3.2
546,562 - Pf6N2E2_480 0.38 -3.1
546,562 - Pf6N2E2_480 0.38 -3.6
546,565 - Pf6N2E2_480 0.38 -3.1
546,645 + Pf6N2E2_480 0.46 -4.0
546,645 + Pf6N2E2_480 0.46 -2.5
546,645 + Pf6N2E2_480 0.46 -2.4
546,646 - Pf6N2E2_480 0.46 -1.6
546,646 - Pf6N2E2_480 0.46 -2.5
546,721 - Pf6N2E2_480 0.53 -1.3
546,804 + Pf6N2E2_480 0.61 -1.9
546,804 + Pf6N2E2_480 0.61 -2.9
546,804 + Pf6N2E2_480 0.61 -1.3
546,804 + Pf6N2E2_480 0.61 -3.0
546,804 + Pf6N2E2_480 0.61 -1.6
546,805 - Pf6N2E2_480 0.61 -3.8
546,805 - Pf6N2E2_480 0.61 -1.5
546,913 - Pf6N2E2_480 0.71 -1.9
546,918 + Pf6N2E2_480 0.71 -2.5
546,918 + Pf6N2E2_480 0.71 -1.9
546,918 + Pf6N2E2_480 0.71 -3.1
546,918 + Pf6N2E2_480 0.71 -1.6
546,919 - Pf6N2E2_480 0.72 -3.9
546,919 - Pf6N2E2_480 0.72 -1.5
546,919 - Pf6N2E2_480 0.72 -2.6
546,919 - Pf6N2E2_480 0.72 -3.6
547,032 + Pf6N2E2_480 0.82 -1.9
547,033 - Pf6N2E2_480 0.82 -2.5
547,033 - Pf6N2E2_480 0.82 -2.5
547,033 - Pf6N2E2_480 0.82 -3.5
547,077 + Pf6N2E2_480 0.86 -3.3
547,078 - -1.3

Or see this region's nucleotide sequence