Experiment: L-Leucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4748 and Pf6N2E2_4749 are separated by 239 nucleotides Pf6N2E2_4749 and Pf6N2E2_4750 are separated by 32 nucleotides Pf6N2E2_4750 and Pf6N2E2_4751 are separated by 252 nucleotides Pf6N2E2_4751 and Pf6N2E2_4752 are separated by 228 nucleotides
Pf6N2E2_4748 - RNA polymerase sigma-70 factor, ECF subfamily, at 5,484,575 to 5,485,024
_4748
Pf6N2E2_4749 - DNA polymerase III epsilon subunit (EC 2.7.7.7), at 5,485,264 to 5,485,935
_4749
Pf6N2E2_4750 - Predicted signal-transduction protein containing cAMP-binding and CBS domains, at 5,485,968 to 5,487,908
_4750
Pf6N2E2_4751 - response regulator, at 5,488,161 to 5,488,607
_4751
Pf6N2E2_4752 - Malate synthase G (EC 2.3.3.9), at 5,488,836 to 5,491,013
_4752
Position (kb)
5485
5486
5487
5488 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 5485.025 kb on + strand at 5485.047 kb on + strand at 5485.047 kb on + strand at 5485.047 kb on + strand at 5485.047 kb on + strand at 5485.048 kb on - strand at 5485.048 kb on - strand at 5485.048 kb on - strand at 5485.049 kb on + strand at 5485.189 kb on + strand at 5485.240 kb on + strand at 5485.240 kb on + strand at 5485.240 kb on + strand at 5485.544 kb on + strand, within Pf6N2E2_4749 at 5485.544 kb on + strand, within Pf6N2E2_4749 at 5485.544 kb on + strand, within Pf6N2E2_4749 at 5485.544 kb on + strand, within Pf6N2E2_4749 at 5485.545 kb on - strand, within Pf6N2E2_4749 at 5485.545 kb on - strand, within Pf6N2E2_4749 at 5485.978 kb on + strand at 5486.084 kb on - strand at 5486.146 kb on + strand at 5486.146 kb on + strand at 5486.146 kb on + strand at 5486.147 kb on - strand at 5486.158 kb on + strand at 5486.159 kb on - strand at 5486.159 kb on - strand at 5486.222 kb on + strand, within Pf6N2E2_4750 at 5486.222 kb on + strand, within Pf6N2E2_4750 at 5486.222 kb on + strand, within Pf6N2E2_4750 at 5486.223 kb on - strand, within Pf6N2E2_4750 at 5486.223 kb on - strand, within Pf6N2E2_4750 at 5486.223 kb on - strand, within Pf6N2E2_4750 at 5486.223 kb on - strand, within Pf6N2E2_4750 at 5486.494 kb on + strand, within Pf6N2E2_4750 at 5486.494 kb on + strand, within Pf6N2E2_4750 at 5486.494 kb on + strand, within Pf6N2E2_4750 at 5486.494 kb on + strand, within Pf6N2E2_4750 at 5486.494 kb on + strand, within Pf6N2E2_4750 at 5486.494 kb on + strand, within Pf6N2E2_4750 at 5486.494 kb on + strand, within Pf6N2E2_4750 at 5486.495 kb on - strand, within Pf6N2E2_4750 at 5486.495 kb on - strand, within Pf6N2E2_4750 at 5486.495 kb on - strand, within Pf6N2E2_4750 at 5486.495 kb on - strand, within Pf6N2E2_4750 at 5486.670 kb on + strand, within Pf6N2E2_4750 at 5486.832 kb on + strand, within Pf6N2E2_4750 at 5486.832 kb on + strand, within Pf6N2E2_4750 at 5486.832 kb on + strand, within Pf6N2E2_4750 at 5486.832 kb on + strand, within Pf6N2E2_4750 at 5486.833 kb on - strand, within Pf6N2E2_4750 at 5486.844 kb on + strand, within Pf6N2E2_4750 at 5486.844 kb on + strand, within Pf6N2E2_4750 at 5486.844 kb on + strand, within Pf6N2E2_4750 at 5486.845 kb on - strand at 5486.845 kb on - strand, within Pf6N2E2_4750 at 5486.845 kb on - strand, within Pf6N2E2_4750 at 5486.845 kb on - strand, within Pf6N2E2_4750 at 5486.950 kb on - strand, within Pf6N2E2_4750 at 5487.168 kb on + strand, within Pf6N2E2_4750 at 5487.169 kb on - strand, within Pf6N2E2_4750 at 5487.169 kb on - strand, within Pf6N2E2_4750 at 5487.169 kb on - strand, within Pf6N2E2_4750 at 5487.169 kb on - strand, within Pf6N2E2_4750 at 5487.386 kb on - strand, within Pf6N2E2_4750 at 5487.653 kb on + strand, within Pf6N2E2_4750 at 5487.654 kb on - strand, within Pf6N2E2_4750 at 5487.670 kb on - strand, within Pf6N2E2_4750 at 5487.694 kb on + strand, within Pf6N2E2_4750 at 5487.694 kb on + strand, within Pf6N2E2_4750 at 5487.694 kb on + strand, within Pf6N2E2_4750 at 5487.694 kb on + strand, within Pf6N2E2_4750 at 5487.694 kb on + strand, within Pf6N2E2_4750 at 5487.694 kb on + strand, within Pf6N2E2_4750 at 5487.695 kb on - strand, within Pf6N2E2_4750 at 5487.695 kb on - strand, within Pf6N2E2_4750 at 5487.695 kb on - strand, within Pf6N2E2_4750 at 5487.695 kb on - strand, within Pf6N2E2_4750 at 5487.749 kb on - strand at 5487.779 kb on - strand at 5487.903 kb on - strand at 5487.909 kb on + strand at 5487.909 kb on + strand at 5487.910 kb on - strand at 5487.987 kb on + strand at 5487.987 kb on + strand at 5487.987 kb on + strand at 5487.987 kb on + strand at 5487.987 kb on + strand at 5487.987 kb on + strand at 5487.987 kb on + strand at 5487.987 kb on + strand at 5487.987 kb on + strand at 5487.987 kb on + strand at 5487.987 kb on + strand at 5487.987 kb on + strand at 5487.987 kb on + strand at 5487.987 kb on + strand at 5487.988 kb on - strand at 5487.988 kb on - strand at 5487.988 kb on - strand at 5487.988 kb on - strand at 5487.988 kb on - strand at 5487.988 kb on - strand at 5487.988 kb on - strand at 5487.988 kb on - strand at 5487.988 kb on - strand at 5487.993 kb on + strand at 5487.994 kb on - strand at 5488.006 kb on - strand at 5488.017 kb on + strand at 5488.018 kb on - strand at 5488.018 kb on - strand at 5488.030 kb on + strand at 5488.057 kb on + strand at 5488.057 kb on + strand at 5488.057 kb on + strand at 5488.057 kb on + strand at 5488.058 kb on - strand at 5488.058 kb on - strand at 5488.058 kb on - strand at 5488.058 kb on - strand at 5488.058 kb on - strand at 5488.141 kb on + strand at 5488.142 kb on - strand at 5488.150 kb on + strand at 5488.150 kb on + strand at 5488.150 kb on + strand at 5488.150 kb on + strand at 5488.150 kb on + strand at 5488.150 kb on + strand at 5488.150 kb on + strand at 5488.150 kb on + strand at 5488.151 kb on - strand at 5488.151 kb on - strand at 5488.175 kb on + strand at 5488.175 kb on + strand at 5488.175 kb on + strand at 5488.176 kb on - strand at 5488.427 kb on + strand, within Pf6N2E2_4751 at 5488.427 kb on + strand, within Pf6N2E2_4751 at 5488.428 kb on - strand, within Pf6N2E2_4751 at 5488.428 kb on - strand, within Pf6N2E2_4751 at 5488.477 kb on + strand, within Pf6N2E2_4751 at 5488.605 kb on + strand at 5488.605 kb on + strand at 5488.695 kb on + strand at 5488.695 kb on + strand at 5488.696 kb on - strand at 5488.772 kb on + strand at 5488.772 kb on + strand at 5488.772 kb on + strand at 5488.772 kb on + strand at 5488.772 kb on + strand at 5488.772 kb on + strand at 5488.773 kb on - strand at 5488.773 kb on - strand at 5488.894 kb on + strand at 5488.895 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Leucine (C) remove 5,485,025 + +2.2 5,485,047 + +0.0 5,485,047 + +0.5 5,485,047 + +0.2 5,485,047 + -0.0 5,485,048 - -0.6 5,485,048 - +0.5 5,485,048 - -1.8 5,485,049 + -0.0 5,485,189 + +0.9 5,485,240 + +0.5 5,485,240 + -0.0 5,485,240 + -0.4 5,485,544 + Pf6N2E2_4749 0.42 +0.3 5,485,544 + Pf6N2E2_4749 0.42 -1.5 5,485,544 + Pf6N2E2_4749 0.42 -0.4 5,485,544 + Pf6N2E2_4749 0.42 -0.3 5,485,545 - Pf6N2E2_4749 0.42 +0.3 5,485,545 - Pf6N2E2_4749 0.42 -1.6 5,485,978 + -0.8 5,486,084 - +1.0 5,486,146 + -1.6 5,486,146 + +0.4 5,486,146 + -0.2 5,486,147 - +1.5 5,486,158 + +0.5 5,486,159 - +0.4 5,486,159 - +0.8 5,486,222 + Pf6N2E2_4750 0.13 +0.6 5,486,222 + Pf6N2E2_4750 0.13 -0.4 5,486,222 + Pf6N2E2_4750 0.13 -0.1 5,486,223 - Pf6N2E2_4750 0.13 -0.1 5,486,223 - Pf6N2E2_4750 0.13 -0.8 5,486,223 - Pf6N2E2_4750 0.13 -0.1 5,486,223 - Pf6N2E2_4750 0.13 +0.6 5,486,494 + Pf6N2E2_4750 0.27 -1.3 5,486,494 + Pf6N2E2_4750 0.27 +1.1 5,486,494 + Pf6N2E2_4750 0.27 -0.5 5,486,494 + Pf6N2E2_4750 0.27 +0.1 5,486,494 + Pf6N2E2_4750 0.27 -1.3 5,486,494 + Pf6N2E2_4750 0.27 +0.0 5,486,494 + Pf6N2E2_4750 0.27 +1.0 5,486,495 - Pf6N2E2_4750 0.27 -0.0 5,486,495 - Pf6N2E2_4750 0.27 -0.3 5,486,495 - Pf6N2E2_4750 0.27 -1.2 5,486,495 - Pf6N2E2_4750 0.27 -0.4 5,486,670 + Pf6N2E2_4750 0.36 +0.1 5,486,832 + Pf6N2E2_4750 0.45 -0.0 5,486,832 + Pf6N2E2_4750 0.45 -0.3 5,486,832 + Pf6N2E2_4750 0.45 -0.2 5,486,832 + Pf6N2E2_4750 0.45 +0.9 5,486,833 - Pf6N2E2_4750 0.45 -1.8 5,486,844 + Pf6N2E2_4750 0.45 -0.2 5,486,844 + Pf6N2E2_4750 0.45 -0.1 5,486,844 + Pf6N2E2_4750 0.45 +1.2 5,486,845 - +0.2 5,486,845 - Pf6N2E2_4750 0.45 -0.1 5,486,845 - Pf6N2E2_4750 0.45 +0.3 5,486,845 - Pf6N2E2_4750 0.45 +0.2 5,486,950 - Pf6N2E2_4750 0.51 -0.7 5,487,168 + Pf6N2E2_4750 0.62 -0.3 5,487,169 - Pf6N2E2_4750 0.62 -0.0 5,487,169 - Pf6N2E2_4750 0.62 +0.1 5,487,169 - Pf6N2E2_4750 0.62 -1.1 5,487,169 - Pf6N2E2_4750 0.62 -0.4 5,487,386 - Pf6N2E2_4750 0.73 -0.4 5,487,653 + Pf6N2E2_4750 0.87 +0.3 5,487,654 - Pf6N2E2_4750 0.87 -0.7 5,487,670 - Pf6N2E2_4750 0.88 -0.1 5,487,694 + Pf6N2E2_4750 0.89 +1.2 5,487,694 + Pf6N2E2_4750 0.89 -0.7 5,487,694 + Pf6N2E2_4750 0.89 +0.8 5,487,694 + Pf6N2E2_4750 0.89 -0.2 5,487,694 + Pf6N2E2_4750 0.89 +0.1 5,487,694 + Pf6N2E2_4750 0.89 +1.3 5,487,695 - Pf6N2E2_4750 0.89 +0.5 5,487,695 - Pf6N2E2_4750 0.89 +0.7 5,487,695 - Pf6N2E2_4750 0.89 +0.8 5,487,695 - Pf6N2E2_4750 0.89 -0.1 5,487,749 - +0.2 5,487,779 - +0.1 5,487,903 - -0.3 5,487,909 + +1.5 5,487,909 + +1.4 5,487,910 - +0.6 5,487,987 + +1.1 5,487,987 + +0.5 5,487,987 + +0.3 5,487,987 + +0.8 5,487,987 + +0.9 5,487,987 + +0.2 5,487,987 + -1.2 5,487,987 + +0.8 5,487,987 + +0.6 5,487,987 + +0.9 5,487,987 + +0.4 5,487,987 + +1.2 5,487,987 + +0.3 5,487,987 + +0.6 5,487,988 - -0.5 5,487,988 - +0.7 5,487,988 - -0.0 5,487,988 - +0.1 5,487,988 - +0.6 5,487,988 - +1.2 5,487,988 - -1.5 5,487,988 - +0.1 5,487,988 - +1.2 5,487,993 + +1.9 5,487,994 - -0.9 5,488,006 - +0.6 5,488,017 + +0.2 5,488,018 - +0.7 5,488,018 - -1.0 5,488,030 + +0.1 5,488,057 + +0.9 5,488,057 + +1.3 5,488,057 + +1.4 5,488,057 + +0.0 5,488,058 - -0.5 5,488,058 - -0.0 5,488,058 - +0.8 5,488,058 - +0.2 5,488,058 - -0.5 5,488,141 + -0.9 5,488,142 - -0.4 5,488,150 + -0.3 5,488,150 + +1.6 5,488,150 + +0.6 5,488,150 + +0.1 5,488,150 + +0.1 5,488,150 + +0.2 5,488,150 + +1.0 5,488,150 + +0.3 5,488,151 - -0.6 5,488,151 - +1.8 5,488,175 + -1.8 5,488,175 + +0.2 5,488,175 + +2.2 5,488,176 - -0.3 5,488,427 + Pf6N2E2_4751 0.60 -0.1 5,488,427 + Pf6N2E2_4751 0.60 -0.4 5,488,428 - Pf6N2E2_4751 0.60 -1.1 5,488,428 - Pf6N2E2_4751 0.60 +0.2 5,488,477 + Pf6N2E2_4751 0.71 +0.5 5,488,605 + +0.5 5,488,605 + -0.4 5,488,695 + +0.7 5,488,695 + +0.5 5,488,696 - -0.5 5,488,772 + -1.0 5,488,772 + -1.1 5,488,772 + -0.0 5,488,772 + -0.8 5,488,772 + -0.3 5,488,772 + -0.4 5,488,773 - -0.3 5,488,773 - -0.3 5,488,894 + -1.0 5,488,895 - -1.8
Or see this region's nucleotide sequence