Experiment: L-Leucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4121 and Pf6N2E2_4122 overlap by 4 nucleotides Pf6N2E2_4122 and Pf6N2E2_4123 are separated by 70 nucleotides Pf6N2E2_4123 and Pf6N2E2_4124 are separated by 167 nucleotides
Pf6N2E2_4121 - Ribosomal RNA small subunit methyltransferase B (EC 2.1.1.-), at 4,800,224 to 4,801,534
_4121
Pf6N2E2_4122 - Methionyl-tRNA formyltransferase (EC 2.1.2.9), at 4,801,531 to 4,802,490
_4122
Pf6N2E2_4123 - Peptide deformylase (EC 3.5.1.88), at 4,802,561 to 4,803,067
_4123
Pf6N2E2_4124 - Uncharacterized protein with LysM domain, COG1652, at 4,803,235 to 4,804,260
_4124
Position (kb)
4801
4802
4803 Strain fitness (log2 ratio)
-2
-1
0
1 at 4800.933 kb on + strand, within Pf6N2E2_4121 at 4800.934 kb on - strand, within Pf6N2E2_4121 at 4800.982 kb on - strand, within Pf6N2E2_4121 at 4802.495 kb on - strand at 4802.495 kb on - strand at 4802.495 kb on - strand at 4802.506 kb on - strand at 4802.506 kb on - strand at 4802.621 kb on - strand, within Pf6N2E2_4123 at 4803.068 kb on - strand at 4803.068 kb on - strand at 4803.077 kb on - strand at 4803.077 kb on - strand at 4803.077 kb on - strand at 4803.077 kb on - strand at 4803.094 kb on - strand at 4803.094 kb on - strand at 4803.143 kb on - strand at 4803.203 kb on - strand at 4803.217 kb on - strand at 4803.217 kb on - strand at 4803.217 kb on - strand at 4803.217 kb on - strand at 4803.217 kb on - strand at 4803.217 kb on - strand at 4803.217 kb on - strand at 4803.217 kb on - strand at 4803.217 kb on - strand at 4803.220 kb on - strand at 4803.249 kb on - strand at 4803.325 kb on + strand at 4803.325 kb on + strand at 4803.325 kb on + strand at 4803.325 kb on + strand at 4803.326 kb on - strand at 4803.326 kb on - strand at 4803.370 kb on + strand, within Pf6N2E2_4124 at 4803.370 kb on + strand, within Pf6N2E2_4124 at 4803.370 kb on + strand, within Pf6N2E2_4124 at 4803.370 kb on + strand, within Pf6N2E2_4124 at 4803.370 kb on + strand, within Pf6N2E2_4124 at 4803.370 kb on + strand, within Pf6N2E2_4124 at 4803.371 kb on - strand, within Pf6N2E2_4124 at 4803.371 kb on - strand, within Pf6N2E2_4124 at 4803.371 kb on - strand, within Pf6N2E2_4124 at 4803.371 kb on - strand, within Pf6N2E2_4124 at 4803.469 kb on + strand, within Pf6N2E2_4124 at 4803.469 kb on + strand, within Pf6N2E2_4124 at 4803.469 kb on + strand, within Pf6N2E2_4124 at 4803.469 kb on + strand, within Pf6N2E2_4124 at 4803.469 kb on + strand, within Pf6N2E2_4124 at 4803.469 kb on + strand, within Pf6N2E2_4124 at 4803.470 kb on - strand, within Pf6N2E2_4124 at 4803.470 kb on - strand, within Pf6N2E2_4124 at 4803.470 kb on - strand, within Pf6N2E2_4124 at 4803.470 kb on - strand, within Pf6N2E2_4124
Per-strain Table
Position Strand Gene LocusTag Fraction L-Leucine (C) remove 4,800,933 + Pf6N2E2_4121 0.54 +0.1 4,800,934 - Pf6N2E2_4121 0.54 +0.2 4,800,982 - Pf6N2E2_4121 0.58 -0.6 4,802,495 - -0.8 4,802,495 - +0.6 4,802,495 - +0.1 4,802,506 - +0.2 4,802,506 - -0.4 4,802,621 - Pf6N2E2_4123 0.12 -2.4 4,803,068 - +1.0 4,803,068 - -0.0 4,803,077 - -0.5 4,803,077 - -0.4 4,803,077 - +0.2 4,803,077 - -0.5 4,803,094 - -0.1 4,803,094 - +0.5 4,803,143 - +0.8 4,803,203 - -0.6 4,803,217 - +0.7 4,803,217 - -0.1 4,803,217 - -0.2 4,803,217 - +0.9 4,803,217 - +0.4 4,803,217 - -0.5 4,803,217 - +0.2 4,803,217 - -0.3 4,803,217 - +0.6 4,803,220 - +0.4 4,803,249 - +0.3 4,803,325 + -0.6 4,803,325 + +0.6 4,803,325 + -2.1 4,803,325 + -0.1 4,803,326 - +0.1 4,803,326 - +0.0 4,803,370 + Pf6N2E2_4124 0.13 -0.1 4,803,370 + Pf6N2E2_4124 0.13 +0.1 4,803,370 + Pf6N2E2_4124 0.13 +0.2 4,803,370 + Pf6N2E2_4124 0.13 -0.3 4,803,370 + Pf6N2E2_4124 0.13 -0.6 4,803,370 + Pf6N2E2_4124 0.13 +0.7 4,803,371 - Pf6N2E2_4124 0.13 -0.5 4,803,371 - Pf6N2E2_4124 0.13 -1.7 4,803,371 - Pf6N2E2_4124 0.13 -0.0 4,803,371 - Pf6N2E2_4124 0.13 -0.4 4,803,469 + Pf6N2E2_4124 0.23 -2.4 4,803,469 + Pf6N2E2_4124 0.23 +0.4 4,803,469 + Pf6N2E2_4124 0.23 +0.9 4,803,469 + Pf6N2E2_4124 0.23 -0.6 4,803,469 + Pf6N2E2_4124 0.23 -0.3 4,803,469 + Pf6N2E2_4124 0.23 +0.1 4,803,470 - Pf6N2E2_4124 0.23 +0.7 4,803,470 - Pf6N2E2_4124 0.23 +0.8 4,803,470 - Pf6N2E2_4124 0.23 -0.2 4,803,470 - Pf6N2E2_4124 0.23 +0.6
Or see this region's nucleotide sequence