Experiment: L-Leucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4021 and Pf6N2E2_4022 are separated by 107 nucleotides Pf6N2E2_4022 and Pf6N2E2_4023 are separated by 19 nucleotides Pf6N2E2_4023 and Pf6N2E2_4024 are separated by 5 nucleotides Pf6N2E2_4024 and Pf6N2E2_4025 are separated by 38 nucleotides Pf6N2E2_4025 and Pf6N2E2_4026 are separated by 155 nucleotides
Pf6N2E2_4021 - Bifunctional protein: zinc-containing alcohol dehydrogenase; quinone oxidoreductase ( NADPH:quinone reductase) (EC 1.1.1.-); Similar to arginate lyase, at 4,694,498 to 4,695,499
_4021
Pf6N2E2_4022 - Cyn operon transcriptional activator, at 4,695,607 to 4,696,155
_4022
Pf6N2E2_4023 - Cyn operon transcriptional activator, at 4,696,175 to 4,696,486
_4023
Pf6N2E2_4024 - L-Proline/Glycine betaine transporter ProP, at 4,696,492 to 4,697,805
_4024
Pf6N2E2_4025 - hypothetical protein, at 4,697,844 to 4,698,017
_4025
Pf6N2E2_4026 - FIG00954152: hypothetical protein, at 4,698,173 to 4,698,733
_4026
Position (kb)
4696
4697
4698 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 4695.506 kb on - strand at 4695.532 kb on + strand at 4695.533 kb on - strand at 4695.533 kb on - strand at 4695.558 kb on - strand at 4695.558 kb on - strand at 4695.575 kb on + strand at 4695.598 kb on + strand at 4695.598 kb on + strand at 4695.802 kb on + strand, within Pf6N2E2_4022 at 4695.802 kb on + strand, within Pf6N2E2_4022 at 4695.803 kb on - strand, within Pf6N2E2_4022 at 4695.821 kb on + strand, within Pf6N2E2_4022 at 4695.821 kb on + strand, within Pf6N2E2_4022 at 4695.821 kb on + strand, within Pf6N2E2_4022 at 4695.821 kb on + strand, within Pf6N2E2_4022 at 4695.821 kb on + strand, within Pf6N2E2_4022 at 4695.822 kb on - strand, within Pf6N2E2_4022 at 4695.822 kb on - strand, within Pf6N2E2_4022 at 4695.822 kb on - strand, within Pf6N2E2_4022 at 4695.822 kb on - strand, within Pf6N2E2_4022 at 4695.822 kb on - strand, within Pf6N2E2_4022 at 4695.822 kb on - strand, within Pf6N2E2_4022 at 4695.973 kb on - strand, within Pf6N2E2_4022 at 4695.986 kb on + strand, within Pf6N2E2_4022 at 4695.987 kb on - strand, within Pf6N2E2_4022 at 4695.987 kb on - strand, within Pf6N2E2_4022 at 4695.987 kb on - strand, within Pf6N2E2_4022 at 4695.987 kb on - strand, within Pf6N2E2_4022 at 4696.102 kb on + strand at 4696.102 kb on + strand at 4696.102 kb on + strand at 4696.103 kb on - strand at 4696.118 kb on + strand at 4696.118 kb on + strand at 4696.118 kb on + strand at 4696.119 kb on - strand at 4696.119 kb on - strand at 4696.119 kb on - strand at 4696.168 kb on + strand at 4696.168 kb on + strand at 4696.169 kb on - strand at 4696.169 kb on - strand at 4696.199 kb on + strand at 4696.199 kb on + strand at 4696.199 kb on + strand at 4696.199 kb on + strand at 4696.199 kb on + strand at 4696.199 kb on + strand at 4696.199 kb on + strand at 4696.199 kb on + strand at 4696.199 kb on + strand at 4696.199 kb on + strand at 4696.200 kb on - strand at 4696.200 kb on - strand at 4696.200 kb on - strand at 4696.547 kb on + strand at 4696.548 kb on - strand at 4696.589 kb on + strand at 4696.664 kb on + strand, within Pf6N2E2_4024 at 4696.664 kb on + strand, within Pf6N2E2_4024 at 4696.676 kb on + strand, within Pf6N2E2_4024 at 4696.677 kb on - strand, within Pf6N2E2_4024 at 4696.677 kb on - strand, within Pf6N2E2_4024 at 4696.677 kb on - strand, within Pf6N2E2_4024 at 4696.677 kb on - strand, within Pf6N2E2_4024 at 4696.820 kb on + strand, within Pf6N2E2_4024 at 4696.856 kb on + strand, within Pf6N2E2_4024 at 4696.856 kb on + strand, within Pf6N2E2_4024 at 4696.856 kb on + strand, within Pf6N2E2_4024 at 4697.255 kb on + strand, within Pf6N2E2_4024 at 4697.255 kb on + strand, within Pf6N2E2_4024 at 4697.255 kb on + strand, within Pf6N2E2_4024 at 4697.255 kb on + strand, within Pf6N2E2_4024 at 4697.256 kb on - strand, within Pf6N2E2_4024 at 4697.256 kb on - strand, within Pf6N2E2_4024 at 4697.298 kb on - strand, within Pf6N2E2_4024 at 4697.336 kb on + strand, within Pf6N2E2_4024 at 4697.337 kb on - strand, within Pf6N2E2_4024 at 4697.421 kb on + strand, within Pf6N2E2_4024 at 4697.454 kb on - strand, within Pf6N2E2_4024 at 4697.454 kb on - strand, within Pf6N2E2_4024 at 4697.468 kb on + strand, within Pf6N2E2_4024 at 4697.712 kb on - strand at 4697.756 kb on + strand at 4697.756 kb on + strand at 4697.756 kb on + strand at 4697.756 kb on + strand at 4697.756 kb on + strand at 4697.756 kb on + strand at 4697.757 kb on - strand at 4697.805 kb on + strand at 4697.806 kb on - strand at 4697.806 kb on - strand at 4697.822 kb on + strand at 4697.822 kb on + strand at 4697.822 kb on + strand at 4697.822 kb on + strand at 4697.822 kb on + strand at 4697.822 kb on + strand at 4697.955 kb on + strand, within Pf6N2E2_4025 at 4697.956 kb on - strand, within Pf6N2E2_4025 at 4698.194 kb on + strand at 4698.194 kb on + strand at 4698.194 kb on + strand at 4698.195 kb on - strand at 4698.195 kb on - strand at 4698.195 kb on - strand at 4698.200 kb on - strand at 4698.307 kb on - strand, within Pf6N2E2_4026 at 4698.349 kb on - strand, within Pf6N2E2_4026 at 4698.349 kb on - strand, within Pf6N2E2_4026 at 4698.349 kb on - strand, within Pf6N2E2_4026 at 4698.373 kb on + strand, within Pf6N2E2_4026 at 4698.373 kb on + strand, within Pf6N2E2_4026 at 4698.374 kb on - strand, within Pf6N2E2_4026 at 4698.374 kb on - strand, within Pf6N2E2_4026 at 4698.510 kb on + strand, within Pf6N2E2_4026 at 4698.644 kb on - strand, within Pf6N2E2_4026 at 4698.644 kb on - strand, within Pf6N2E2_4026 at 4698.734 kb on - strand at 4698.779 kb on + strand at 4698.779 kb on + strand at 4698.779 kb on + strand at 4698.779 kb on + strand at 4698.779 kb on + strand at 4698.779 kb on + strand at 4698.779 kb on + strand at 4698.779 kb on + strand at 4698.779 kb on + strand at 4698.779 kb on + strand at 4698.780 kb on - strand at 4698.780 kb on - strand at 4698.780 kb on - strand at 4698.780 kb on - strand at 4698.780 kb on - strand at 4698.780 kb on - strand at 4698.780 kb on - strand at 4698.780 kb on - strand at 4698.781 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Leucine (C) remove 4,695,506 - +0.2 4,695,532 + -0.0 4,695,533 - +0.5 4,695,533 - -0.5 4,695,558 - +1.3 4,695,558 - -1.3 4,695,575 + -0.1 4,695,598 + -1.3 4,695,598 + -0.7 4,695,802 + Pf6N2E2_4022 0.36 -0.0 4,695,802 + Pf6N2E2_4022 0.36 +1.0 4,695,803 - Pf6N2E2_4022 0.36 +0.3 4,695,821 + Pf6N2E2_4022 0.39 -0.3 4,695,821 + Pf6N2E2_4022 0.39 -0.1 4,695,821 + Pf6N2E2_4022 0.39 +0.6 4,695,821 + Pf6N2E2_4022 0.39 +0.5 4,695,821 + Pf6N2E2_4022 0.39 -1.0 4,695,822 - Pf6N2E2_4022 0.39 -1.1 4,695,822 - Pf6N2E2_4022 0.39 -0.0 4,695,822 - Pf6N2E2_4022 0.39 +0.7 4,695,822 - Pf6N2E2_4022 0.39 +0.7 4,695,822 - Pf6N2E2_4022 0.39 -0.7 4,695,822 - Pf6N2E2_4022 0.39 -0.5 4,695,973 - Pf6N2E2_4022 0.67 -0.8 4,695,986 + Pf6N2E2_4022 0.69 +0.5 4,695,987 - Pf6N2E2_4022 0.69 -0.1 4,695,987 - Pf6N2E2_4022 0.69 +0.4 4,695,987 - Pf6N2E2_4022 0.69 -0.4 4,695,987 - Pf6N2E2_4022 0.69 +1.0 4,696,102 + +0.2 4,696,102 + +0.1 4,696,102 + -0.6 4,696,103 - +0.7 4,696,118 + -1.4 4,696,118 + +4.3 4,696,118 + -0.5 4,696,119 - -1.1 4,696,119 - +0.9 4,696,119 - +0.2 4,696,168 + -0.4 4,696,168 + +0.4 4,696,169 - -0.1 4,696,169 - -2.5 4,696,199 + +0.2 4,696,199 + +0.1 4,696,199 + +0.5 4,696,199 + +0.4 4,696,199 + +0.2 4,696,199 + -0.7 4,696,199 + -2.6 4,696,199 + +0.2 4,696,199 + -1.1 4,696,199 + +0.1 4,696,200 - +1.4 4,696,200 - +0.5 4,696,200 - -0.4 4,696,547 + +0.0 4,696,548 - -0.2 4,696,589 + -2.1 4,696,664 + Pf6N2E2_4024 0.13 -2.6 4,696,664 + Pf6N2E2_4024 0.13 -2.6 4,696,676 + Pf6N2E2_4024 0.14 -2.0 4,696,677 - Pf6N2E2_4024 0.14 -0.0 4,696,677 - Pf6N2E2_4024 0.14 -0.6 4,696,677 - Pf6N2E2_4024 0.14 -0.4 4,696,677 - Pf6N2E2_4024 0.14 -1.5 4,696,820 + Pf6N2E2_4024 0.25 -0.7 4,696,856 + Pf6N2E2_4024 0.28 -1.5 4,696,856 + Pf6N2E2_4024 0.28 -0.9 4,696,856 + Pf6N2E2_4024 0.28 +1.1 4,697,255 + Pf6N2E2_4024 0.58 +0.0 4,697,255 + Pf6N2E2_4024 0.58 -1.5 4,697,255 + Pf6N2E2_4024 0.58 -1.3 4,697,255 + Pf6N2E2_4024 0.58 -1.5 4,697,256 - Pf6N2E2_4024 0.58 -0.1 4,697,256 - Pf6N2E2_4024 0.58 -1.3 4,697,298 - Pf6N2E2_4024 0.61 -1.1 4,697,336 + Pf6N2E2_4024 0.64 -1.0 4,697,337 - Pf6N2E2_4024 0.64 -1.8 4,697,421 + Pf6N2E2_4024 0.71 -1.7 4,697,454 - Pf6N2E2_4024 0.73 -0.3 4,697,454 - Pf6N2E2_4024 0.73 -2.1 4,697,468 + Pf6N2E2_4024 0.74 -1.1 4,697,712 - -0.4 4,697,756 + -1.5 4,697,756 + -0.6 4,697,756 + -2.2 4,697,756 + -2.0 4,697,756 + -0.7 4,697,756 + -1.1 4,697,757 - -0.8 4,697,805 + -0.6 4,697,806 - +0.2 4,697,806 - -0.5 4,697,822 + -1.3 4,697,822 + -0.5 4,697,822 + +0.0 4,697,822 + -0.9 4,697,822 + -0.8 4,697,822 + -0.7 4,697,955 + Pf6N2E2_4025 0.64 -2.4 4,697,956 - Pf6N2E2_4025 0.64 -0.9 4,698,194 + +2.0 4,698,194 + +0.6 4,698,194 + +0.0 4,698,195 - +1.5 4,698,195 - +0.5 4,698,195 - +0.7 4,698,200 - -0.6 4,698,307 - Pf6N2E2_4026 0.24 -0.8 4,698,349 - Pf6N2E2_4026 0.31 +1.2 4,698,349 - Pf6N2E2_4026 0.31 -1.7 4,698,349 - Pf6N2E2_4026 0.31 -0.2 4,698,373 + Pf6N2E2_4026 0.36 -0.3 4,698,373 + Pf6N2E2_4026 0.36 +0.8 4,698,374 - Pf6N2E2_4026 0.36 -0.3 4,698,374 - Pf6N2E2_4026 0.36 -0.8 4,698,510 + Pf6N2E2_4026 0.60 -0.6 4,698,644 - Pf6N2E2_4026 0.84 +0.8 4,698,644 - Pf6N2E2_4026 0.84 -0.5 4,698,734 - +0.7 4,698,779 + +0.9 4,698,779 + +0.1 4,698,779 + -0.6 4,698,779 + +0.6 4,698,779 + +0.5 4,698,779 + +0.7 4,698,779 + -0.1 4,698,779 + -0.2 4,698,779 + +0.2 4,698,779 + -0.1 4,698,780 - +0.3 4,698,780 - +0.3 4,698,780 - -1.0 4,698,780 - +0.4 4,698,780 - -0.1 4,698,780 - +0.1 4,698,780 - -1.0 4,698,780 - -1.6 4,698,781 + +0.5
Or see this region's nucleotide sequence