Experiment: L-Leucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3575 and Pf6N2E2_3576 are separated by 765 nucleotides Pf6N2E2_3576 and Pf6N2E2_3577 overlap by 4 nucleotides Pf6N2E2_3577 and Pf6N2E2_3578 overlap by 4 nucleotides
Pf6N2E2_3575 - Oxidoreductase, short chain dehydrogenase/reductase family, at 4,165,475 to 4,166,239
_3575
Pf6N2E2_3576 - Branched-chain amino acid transport ATP-binding protein LivF (TC 3.A.1.4.1), at 4,167,005 to 4,167,721
_3576
Pf6N2E2_3577 - ABC transporter for L-leucine/L-isoleucine/D-alanine, ATPase component 1 LivG (from data) , at 4,167,718 to 4,168,590
_3577
Pf6N2E2_3578 - Branched-chain amino acid transport system permease protein LivM (TC 3.A.1.4.1), at 4,168,587 to 4,169,867
_3578
Position (kb)
4167
4168 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 4166.222 kb on - strand at 4166.222 kb on - strand at 4166.222 kb on - strand at 4166.550 kb on + strand at 4166.550 kb on + strand at 4166.551 kb on - strand at 4166.551 kb on - strand at 4166.553 kb on - strand at 4166.553 kb on - strand at 4166.634 kb on + strand at 4166.635 kb on - strand at 4166.714 kb on - strand at 4166.846 kb on + strand at 4166.846 kb on + strand at 4166.846 kb on + strand at 4166.861 kb on + strand at 4166.862 kb on - strand at 4167.021 kb on + strand at 4167.021 kb on + strand at 4167.028 kb on + strand at 4167.028 kb on + strand at 4167.099 kb on + strand, within Pf6N2E2_3576 at 4167.099 kb on + strand, within Pf6N2E2_3576 at 4167.100 kb on - strand, within Pf6N2E2_3576 at 4167.100 kb on - strand, within Pf6N2E2_3576 at 4167.100 kb on - strand, within Pf6N2E2_3576 at 4167.100 kb on - strand, within Pf6N2E2_3576 at 4167.100 kb on - strand, within Pf6N2E2_3576 at 4167.100 kb on - strand, within Pf6N2E2_3576 at 4167.100 kb on - strand, within Pf6N2E2_3576 at 4167.387 kb on + strand, within Pf6N2E2_3576 at 4167.387 kb on + strand, within Pf6N2E2_3576 at 4167.387 kb on + strand, within Pf6N2E2_3576 at 4167.388 kb on - strand, within Pf6N2E2_3576 at 4167.495 kb on + strand, within Pf6N2E2_3576 at 4167.495 kb on + strand, within Pf6N2E2_3576 at 4167.495 kb on + strand, within Pf6N2E2_3576 at 4167.495 kb on + strand, within Pf6N2E2_3576 at 4167.495 kb on + strand, within Pf6N2E2_3576 at 4167.496 kb on - strand, within Pf6N2E2_3576 at 4167.531 kb on + strand, within Pf6N2E2_3576 at 4167.531 kb on + strand, within Pf6N2E2_3576 at 4167.531 kb on + strand, within Pf6N2E2_3576 at 4167.531 kb on + strand, within Pf6N2E2_3576 at 4167.531 kb on + strand, within Pf6N2E2_3576 at 4167.531 kb on + strand, within Pf6N2E2_3576 at 4167.532 kb on - strand, within Pf6N2E2_3576 at 4167.532 kb on - strand, within Pf6N2E2_3576 at 4167.532 kb on - strand, within Pf6N2E2_3576 at 4167.532 kb on - strand, within Pf6N2E2_3576 at 4167.532 kb on - strand, within Pf6N2E2_3576 at 4167.678 kb on + strand at 4167.679 kb on - strand at 4167.709 kb on + strand at 4167.721 kb on + strand at 4167.722 kb on - strand at 4167.722 kb on - strand at 4167.850 kb on + strand, within Pf6N2E2_3577 at 4167.851 kb on - strand, within Pf6N2E2_3577 at 4168.034 kb on + strand, within Pf6N2E2_3577 at 4168.094 kb on + strand, within Pf6N2E2_3577 at 4168.094 kb on + strand, within Pf6N2E2_3577 at 4168.095 kb on - strand, within Pf6N2E2_3577 at 4168.095 kb on - strand, within Pf6N2E2_3577 at 4168.095 kb on - strand, within Pf6N2E2_3577 at 4168.095 kb on - strand, within Pf6N2E2_3577 at 4168.248 kb on + strand, within Pf6N2E2_3577 at 4168.248 kb on + strand, within Pf6N2E2_3577 at 4168.248 kb on + strand, within Pf6N2E2_3577 at 4168.248 kb on + strand, within Pf6N2E2_3577 at 4168.249 kb on - strand, within Pf6N2E2_3577 at 4168.298 kb on + strand, within Pf6N2E2_3577 at 4168.298 kb on + strand, within Pf6N2E2_3577 at 4168.299 kb on - strand, within Pf6N2E2_3577 at 4168.299 kb on - strand, within Pf6N2E2_3577 at 4168.301 kb on + strand, within Pf6N2E2_3577 at 4168.301 kb on + strand, within Pf6N2E2_3577 at 4168.302 kb on - strand, within Pf6N2E2_3577 at 4168.302 kb on - strand, within Pf6N2E2_3577 at 4168.302 kb on - strand, within Pf6N2E2_3577 at 4168.302 kb on - strand, within Pf6N2E2_3577 at 4168.427 kb on + strand, within Pf6N2E2_3577 at 4168.427 kb on + strand, within Pf6N2E2_3577 at 4168.428 kb on - strand, within Pf6N2E2_3577 at 4168.576 kb on + strand at 4168.677 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Leucine (C) remove 4,166,222 - -1.0 4,166,222 - +0.5 4,166,222 - +0.5 4,166,550 + +1.1 4,166,550 + -0.2 4,166,551 - +0.3 4,166,551 - +0.4 4,166,553 - +0.5 4,166,553 - -0.7 4,166,634 + +0.2 4,166,635 - +0.0 4,166,714 - -0.1 4,166,846 + +0.3 4,166,846 + +0.4 4,166,846 + +0.7 4,166,861 + +0.2 4,166,862 - -0.2 4,167,021 + -0.1 4,167,021 + -2.1 4,167,028 + -0.9 4,167,028 + -1.9 4,167,099 + Pf6N2E2_3576 0.13 -2.2 4,167,099 + Pf6N2E2_3576 0.13 -2.8 4,167,100 - Pf6N2E2_3576 0.13 -2.1 4,167,100 - Pf6N2E2_3576 0.13 +0.5 4,167,100 - Pf6N2E2_3576 0.13 -1.4 4,167,100 - Pf6N2E2_3576 0.13 -3.1 4,167,100 - Pf6N2E2_3576 0.13 -2.7 4,167,100 - Pf6N2E2_3576 0.13 -2.8 4,167,100 - Pf6N2E2_3576 0.13 -1.0 4,167,387 + Pf6N2E2_3576 0.53 -1.9 4,167,387 + Pf6N2E2_3576 0.53 -1.9 4,167,387 + Pf6N2E2_3576 0.53 -0.9 4,167,388 - Pf6N2E2_3576 0.53 -2.0 4,167,495 + Pf6N2E2_3576 0.68 -3.6 4,167,495 + Pf6N2E2_3576 0.68 -0.5 4,167,495 + Pf6N2E2_3576 0.68 -2.8 4,167,495 + Pf6N2E2_3576 0.68 -1.8 4,167,495 + Pf6N2E2_3576 0.68 -1.6 4,167,496 - Pf6N2E2_3576 0.68 -2.6 4,167,531 + Pf6N2E2_3576 0.73 -1.9 4,167,531 + Pf6N2E2_3576 0.73 -2.5 4,167,531 + Pf6N2E2_3576 0.73 -0.1 4,167,531 + Pf6N2E2_3576 0.73 -1.0 4,167,531 + Pf6N2E2_3576 0.73 -0.8 4,167,531 + Pf6N2E2_3576 0.73 -2.9 4,167,532 - Pf6N2E2_3576 0.74 -1.5 4,167,532 - Pf6N2E2_3576 0.74 -1.8 4,167,532 - Pf6N2E2_3576 0.74 -2.4 4,167,532 - Pf6N2E2_3576 0.74 -0.5 4,167,532 - Pf6N2E2_3576 0.74 -1.2 4,167,678 + -1.9 4,167,679 - -2.1 4,167,709 + -2.2 4,167,721 + -1.7 4,167,722 - -2.4 4,167,722 - -0.8 4,167,850 + Pf6N2E2_3577 0.15 -3.7 4,167,851 - Pf6N2E2_3577 0.15 -2.6 4,168,034 + Pf6N2E2_3577 0.36 -1.8 4,168,094 + Pf6N2E2_3577 0.43 -2.1 4,168,094 + Pf6N2E2_3577 0.43 -0.7 4,168,095 - Pf6N2E2_3577 0.43 -1.8 4,168,095 - Pf6N2E2_3577 0.43 -1.7 4,168,095 - Pf6N2E2_3577 0.43 -2.3 4,168,095 - Pf6N2E2_3577 0.43 -4.4 4,168,248 + Pf6N2E2_3577 0.61 -2.5 4,168,248 + Pf6N2E2_3577 0.61 -0.5 4,168,248 + Pf6N2E2_3577 0.61 -2.2 4,168,248 + Pf6N2E2_3577 0.61 -0.3 4,168,249 - Pf6N2E2_3577 0.61 -2.1 4,168,298 + Pf6N2E2_3577 0.66 -1.5 4,168,298 + Pf6N2E2_3577 0.66 -2.1 4,168,299 - Pf6N2E2_3577 0.67 -3.0 4,168,299 - Pf6N2E2_3577 0.67 +0.2 4,168,301 + Pf6N2E2_3577 0.67 -1.6 4,168,301 + Pf6N2E2_3577 0.67 -0.2 4,168,302 - Pf6N2E2_3577 0.67 -2.9 4,168,302 - Pf6N2E2_3577 0.67 -1.6 4,168,302 - Pf6N2E2_3577 0.67 +0.9 4,168,302 - Pf6N2E2_3577 0.67 -0.9 4,168,427 + Pf6N2E2_3577 0.81 -2.3 4,168,427 + Pf6N2E2_3577 0.81 -1.6 4,168,428 - Pf6N2E2_3577 0.81 -1.6 4,168,576 + -2.2 4,168,677 + -2.5
Or see this region's nucleotide sequence