Experiment: L-Leucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_352 and Pf6N2E2_353 are separated by 166 nucleotides Pf6N2E2_353 and Pf6N2E2_354 overlap by 11 nucleotides Pf6N2E2_354 and Pf6N2E2_355 are separated by 152 nucleotides
Pf6N2E2_352 - Opine oxidase subunit B, at 417,890 to 419,071
_352
Pf6N2E2_353 - Bona fide RidA/YjgF/TdcF/RutC subgroup, at 419,238 to 419,642
_353
Pf6N2E2_354 - Type III PLP / low-specificity D-threonine aldolase, at 419,632 to 420,711
_354
Pf6N2E2_355 - Transcriptional regulator, LysR family, at 420,864 to 421,748
_355
Position (kb) 419
420
421 Strain fitness (log2 ratio) -2
-1
0
1 at 418.653 kb on + strand, within Pf6N2E2_352 at 418.654 kb on - strand, within Pf6N2E2_352 at 418.870 kb on - strand, within Pf6N2E2_352 at 418.870 kb on - strand, within Pf6N2E2_352 at 418.948 kb on - strand, within Pf6N2E2_352 at 419.048 kb on + strand at 419.048 kb on + strand at 419.048 kb on + strand at 419.048 kb on + strand at 419.049 kb on - strand at 419.049 kb on - strand at 419.049 kb on - strand at 419.075 kb on - strand at 419.112 kb on + strand at 419.112 kb on + strand at 419.112 kb on + strand at 419.112 kb on + strand at 419.112 kb on + strand at 419.112 kb on + strand at 419.112 kb on + strand at 419.112 kb on + strand at 419.112 kb on + strand at 419.113 kb on - strand at 419.113 kb on - strand at 419.113 kb on - strand at 419.113 kb on - strand at 419.113 kb on - strand at 419.113 kb on - strand at 419.239 kb on + strand at 419.239 kb on + strand at 419.240 kb on - strand at 419.291 kb on - strand, within Pf6N2E2_353 at 419.291 kb on - strand, within Pf6N2E2_353 at 419.351 kb on - strand, within Pf6N2E2_353 at 419.359 kb on + strand, within Pf6N2E2_353 at 419.401 kb on + strand, within Pf6N2E2_353 at 419.401 kb on + strand, within Pf6N2E2_353 at 419.401 kb on + strand, within Pf6N2E2_353 at 419.401 kb on + strand, within Pf6N2E2_353 at 419.401 kb on + strand, within Pf6N2E2_353 at 419.402 kb on - strand, within Pf6N2E2_353 at 419.402 kb on - strand, within Pf6N2E2_353 at 419.402 kb on - strand, within Pf6N2E2_353 at 419.402 kb on - strand, within Pf6N2E2_353 at 419.551 kb on + strand, within Pf6N2E2_353 at 419.551 kb on + strand, within Pf6N2E2_353 at 419.552 kb on - strand, within Pf6N2E2_353 at 419.552 kb on - strand, within Pf6N2E2_353 at 419.552 kb on - strand, within Pf6N2E2_353 at 419.552 kb on - strand, within Pf6N2E2_353 at 419.584 kb on + strand, within Pf6N2E2_353 at 419.585 kb on - strand, within Pf6N2E2_353 at 419.691 kb on - strand at 419.691 kb on - strand at 419.691 kb on - strand at 420.059 kb on + strand, within Pf6N2E2_354 at 420.059 kb on + strand, within Pf6N2E2_354 at 420.059 kb on + strand, within Pf6N2E2_354 at 420.059 kb on + strand, within Pf6N2E2_354 at 420.059 kb on + strand, within Pf6N2E2_354 at 420.060 kb on - strand, within Pf6N2E2_354 at 420.060 kb on - strand, within Pf6N2E2_354 at 420.060 kb on - strand, within Pf6N2E2_354 at 420.242 kb on + strand, within Pf6N2E2_354 at 420.242 kb on + strand, within Pf6N2E2_354 at 420.243 kb on - strand, within Pf6N2E2_354 at 420.521 kb on + strand, within Pf6N2E2_354 at 420.522 kb on - strand, within Pf6N2E2_354 at 420.522 kb on - strand at 420.700 kb on - strand at 420.721 kb on - strand at 420.804 kb on + strand at 420.804 kb on + strand at 420.805 kb on - strand at 421.225 kb on + strand, within Pf6N2E2_355 at 421.225 kb on + strand, within Pf6N2E2_355 at 421.225 kb on + strand, within Pf6N2E2_355 at 421.225 kb on + strand, within Pf6N2E2_355 at 421.225 kb on + strand, within Pf6N2E2_355 at 421.226 kb on - strand, within Pf6N2E2_355 at 421.226 kb on - strand, within Pf6N2E2_355 at 421.427 kb on + strand, within Pf6N2E2_355 at 421.427 kb on + strand, within Pf6N2E2_355 at 421.427 kb on + strand, within Pf6N2E2_355 at 421.427 kb on + strand, within Pf6N2E2_355 at 421.427 kb on + strand, within Pf6N2E2_355 at 421.428 kb on - strand, within Pf6N2E2_355 at 421.428 kb on - strand, within Pf6N2E2_355
Per-strain Table
Position Strand Gene LocusTag Fraction L-Leucine (C) remove 418,653 + Pf6N2E2_352 0.65 +0.2 418,654 - Pf6N2E2_352 0.65 +1.2 418,870 - Pf6N2E2_352 0.83 +0.2 418,870 - Pf6N2E2_352 0.83 +0.2 418,948 - Pf6N2E2_352 0.90 -1.4 419,048 + -0.6 419,048 + -1.5 419,048 + +0.8 419,048 + +0.4 419,049 - -0.1 419,049 - -0.8 419,049 - -2.1 419,075 - -0.7 419,112 + +0.2 419,112 + -0.8 419,112 + -0.5 419,112 + -0.9 419,112 + +0.1 419,112 + -0.2 419,112 + +0.3 419,112 + -1.1 419,112 + -0.4 419,113 - +0.3 419,113 - +1.3 419,113 - +0.6 419,113 - +1.4 419,113 - +0.5 419,113 - +1.2 419,239 + -1.0 419,239 + +0.7 419,240 - +1.6 419,291 - Pf6N2E2_353 0.13 +0.3 419,291 - Pf6N2E2_353 0.13 -0.1 419,351 - Pf6N2E2_353 0.28 +0.2 419,359 + Pf6N2E2_353 0.30 -1.0 419,401 + Pf6N2E2_353 0.40 -0.2 419,401 + Pf6N2E2_353 0.40 +0.4 419,401 + Pf6N2E2_353 0.40 -1.0 419,401 + Pf6N2E2_353 0.40 -1.7 419,401 + Pf6N2E2_353 0.40 +0.4 419,402 - Pf6N2E2_353 0.40 -0.3 419,402 - Pf6N2E2_353 0.40 -0.1 419,402 - Pf6N2E2_353 0.40 +0.8 419,402 - Pf6N2E2_353 0.40 -0.1 419,551 + Pf6N2E2_353 0.77 +1.3 419,551 + Pf6N2E2_353 0.77 +0.3 419,552 - Pf6N2E2_353 0.78 +0.4 419,552 - Pf6N2E2_353 0.78 +0.8 419,552 - Pf6N2E2_353 0.78 +1.1 419,552 - Pf6N2E2_353 0.78 +0.1 419,584 + Pf6N2E2_353 0.85 -0.2 419,585 - Pf6N2E2_353 0.86 +0.7 419,691 - +0.1 419,691 - -0.1 419,691 - +0.3 420,059 + Pf6N2E2_354 0.40 +0.0 420,059 + Pf6N2E2_354 0.40 +0.4 420,059 + Pf6N2E2_354 0.40 +0.2 420,059 + Pf6N2E2_354 0.40 -0.4 420,059 + Pf6N2E2_354 0.40 -0.1 420,060 - Pf6N2E2_354 0.40 +0.1 420,060 - Pf6N2E2_354 0.40 +1.2 420,060 - Pf6N2E2_354 0.40 -0.5 420,242 + Pf6N2E2_354 0.56 +1.2 420,242 + Pf6N2E2_354 0.56 +0.8 420,243 - Pf6N2E2_354 0.57 -0.9 420,521 + Pf6N2E2_354 0.82 -0.5 420,522 - Pf6N2E2_354 0.82 -0.5 420,522 - +0.8 420,700 - +1.1 420,721 - -1.5 420,804 + +0.8 420,804 + +0.1 420,805 - -0.4 421,225 + Pf6N2E2_355 0.41 +0.4 421,225 + Pf6N2E2_355 0.41 -1.5 421,225 + Pf6N2E2_355 0.41 +0.8 421,225 + Pf6N2E2_355 0.41 -0.6 421,225 + Pf6N2E2_355 0.41 -1.1 421,226 - Pf6N2E2_355 0.41 +0.8 421,226 - Pf6N2E2_355 0.41 +0.9 421,427 + Pf6N2E2_355 0.64 +0.1 421,427 + Pf6N2E2_355 0.64 -0.3 421,427 + Pf6N2E2_355 0.64 +0.4 421,427 + Pf6N2E2_355 0.64 +0.4 421,427 + Pf6N2E2_355 0.64 -1.1 421,428 - Pf6N2E2_355 0.64 +0.5 421,428 - Pf6N2E2_355 0.64 -1.2
Or see this region's nucleotide sequence