Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_3383

Experiment: L-Leucine (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_3381 and Pf6N2E2_3382 overlap by 4 nucleotidesPf6N2E2_3382 and Pf6N2E2_3383 are separated by 167 nucleotidesPf6N2E2_3383 and Pf6N2E2_3384 overlap by 4 nucleotidesPf6N2E2_3384 and Pf6N2E2_3385 overlap by 4 nucleotides Pf6N2E2_3381 - Predicted L-lactate dehydrogenase, Fe-S oxidoreductase subunit YkgE, at 3,912,209 to 3,913,033 _3381 Pf6N2E2_3382 - Predicted L-lactate dehydrogenase, Iron-sulfur cluster-binding subunit YkgF, at 3,913,030 to 3,913,593 _3382 Pf6N2E2_3383 - Predicted L-lactate dehydrogenase, Iron-sulfur cluster-binding subunit YkgF, at 3,913,761 to 3,914,489 _3383 Pf6N2E2_3384 - Predicted L-lactate dehydrogenase, hypothetical protein subunit YkgG, at 3,914,486 to 3,915,157 _3384 Pf6N2E2_3385 - Predicted D-lactate dehydrogenase, Fe-S protein, FAD/FMN-containing, at 3,915,154 to 3,917,964 _3385 Position (kb) 3913 3914 3915Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 3913.469 kb on + strand, within Pf6N2E2_3382at 3913.469 kb on + strand, within Pf6N2E2_3382at 3913.469 kb on + strand, within Pf6N2E2_3382at 3913.469 kb on + strand, within Pf6N2E2_3382at 3913.469 kb on + strand, within Pf6N2E2_3382at 3913.470 kb on - strand, within Pf6N2E2_3382at 3913.470 kb on - strand, within Pf6N2E2_3382at 3913.470 kb on - strand, within Pf6N2E2_3382at 3913.470 kb on - strand, within Pf6N2E2_3382at 3913.470 kb on - strand, within Pf6N2E2_3382at 3913.510 kb on + strand, within Pf6N2E2_3382at 3913.510 kb on + strand, within Pf6N2E2_3382at 3913.510 kb on + strand, within Pf6N2E2_3382at 3913.510 kb on + strand, within Pf6N2E2_3382at 3913.510 kb on + strand, within Pf6N2E2_3382at 3913.510 kb on + strand, within Pf6N2E2_3382at 3913.510 kb on + strand, within Pf6N2E2_3382at 3913.511 kb on - strand, within Pf6N2E2_3382at 3913.511 kb on - strand, within Pf6N2E2_3382at 3913.511 kb on - strand, within Pf6N2E2_3382at 3913.578 kb on - strandat 3913.578 kb on - strandat 3913.620 kb on + strandat 3913.620 kb on + strandat 3913.620 kb on + strandat 3913.621 kb on - strandat 3913.621 kb on - strandat 3913.621 kb on - strandat 3913.857 kb on + strand, within Pf6N2E2_3383at 3913.857 kb on + strand, within Pf6N2E2_3383at 3913.858 kb on - strand, within Pf6N2E2_3383at 3913.858 kb on - strand, within Pf6N2E2_3383at 3913.858 kb on - strand, within Pf6N2E2_3383at 3914.006 kb on + strand, within Pf6N2E2_3383at 3914.006 kb on + strand, within Pf6N2E2_3383at 3914.007 kb on - strand, within Pf6N2E2_3383at 3914.007 kb on - strand, within Pf6N2E2_3383at 3914.025 kb on + strandat 3914.025 kb on + strand, within Pf6N2E2_3383at 3914.026 kb on - strand, within Pf6N2E2_3383at 3914.026 kb on - strand, within Pf6N2E2_3383at 3914.062 kb on - strand, within Pf6N2E2_3383at 3914.073 kb on - strand, within Pf6N2E2_3383at 3914.271 kb on + strand, within Pf6N2E2_3383at 3914.272 kb on - strand, within Pf6N2E2_3383at 3914.572 kb on - strand, within Pf6N2E2_3384at 3914.572 kb on - strand, within Pf6N2E2_3384at 3914.585 kb on + strand, within Pf6N2E2_3384at 3915.002 kb on + strand, within Pf6N2E2_3384at 3915.002 kb on + strand, within Pf6N2E2_3384at 3915.002 kb on + strand, within Pf6N2E2_3384at 3915.003 kb on - strand, within Pf6N2E2_3384at 3915.003 kb on - strand, within Pf6N2E2_3384at 3915.003 kb on - strand, within Pf6N2E2_3384at 3915.176 kb on + strandat 3915.176 kb on + strandat 3915.176 kb on + strandat 3915.176 kb on + strandat 3915.177 kb on - strandat 3915.288 kb on - strandat 3915.426 kb on + strandat 3915.426 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Leucine (C)
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3,913,469 + Pf6N2E2_3382 0.78 -3.0
3,913,469 + Pf6N2E2_3382 0.78 -0.8
3,913,469 + Pf6N2E2_3382 0.78 -0.9
3,913,469 + Pf6N2E2_3382 0.78 -0.1
3,913,469 + Pf6N2E2_3382 0.78 -1.2
3,913,470 - Pf6N2E2_3382 0.78 -0.9
3,913,470 - Pf6N2E2_3382 0.78 +0.4
3,913,470 - Pf6N2E2_3382 0.78 +1.0
3,913,470 - Pf6N2E2_3382 0.78 +0.4
3,913,470 - Pf6N2E2_3382 0.78 +0.5
3,913,510 + Pf6N2E2_3382 0.85 -2.1
3,913,510 + Pf6N2E2_3382 0.85 -0.3
3,913,510 + Pf6N2E2_3382 0.85 +0.4
3,913,510 + Pf6N2E2_3382 0.85 -0.3
3,913,510 + Pf6N2E2_3382 0.85 -0.0
3,913,510 + Pf6N2E2_3382 0.85 -0.4
3,913,510 + Pf6N2E2_3382 0.85 +1.3
3,913,511 - Pf6N2E2_3382 0.85 -0.6
3,913,511 - Pf6N2E2_3382 0.85 -0.2
3,913,511 - Pf6N2E2_3382 0.85 +0.0
3,913,578 - +0.3
3,913,578 - -0.7
3,913,620 + +1.3
3,913,620 + +0.7
3,913,620 + -1.3
3,913,621 - +0.5
3,913,621 - -0.2
3,913,621 - -0.2
3,913,857 + Pf6N2E2_3383 0.13 +0.6
3,913,857 + Pf6N2E2_3383 0.13 +0.4
3,913,858 - Pf6N2E2_3383 0.13 -1.7
3,913,858 - Pf6N2E2_3383 0.13 +1.2
3,913,858 - Pf6N2E2_3383 0.13 +0.2
3,914,006 + Pf6N2E2_3383 0.34 -0.1
3,914,006 + Pf6N2E2_3383 0.34 -0.0
3,914,007 - Pf6N2E2_3383 0.34 -0.8
3,914,007 - Pf6N2E2_3383 0.34 +0.3
3,914,025 + +1.4
3,914,025 + Pf6N2E2_3383 0.36 -0.1
3,914,026 - Pf6N2E2_3383 0.36 +1.2
3,914,026 - Pf6N2E2_3383 0.36 +0.7
3,914,062 - Pf6N2E2_3383 0.41 +0.9
3,914,073 - Pf6N2E2_3383 0.43 +0.2
3,914,271 + Pf6N2E2_3383 0.70 +0.5
3,914,272 - Pf6N2E2_3383 0.70 +2.2
3,914,572 - Pf6N2E2_3384 0.13 -0.5
3,914,572 - Pf6N2E2_3384 0.13 -0.0
3,914,585 + Pf6N2E2_3384 0.15 -0.9
3,915,002 + Pf6N2E2_3384 0.77 -0.3
3,915,002 + Pf6N2E2_3384 0.77 +0.1
3,915,002 + Pf6N2E2_3384 0.77 +0.3
3,915,003 - Pf6N2E2_3384 0.77 -1.8
3,915,003 - Pf6N2E2_3384 0.77 +0.7
3,915,003 - Pf6N2E2_3384 0.77 +0.3
3,915,176 + -0.8
3,915,176 + -0.9
3,915,176 + -0.5
3,915,176 + -1.0
3,915,177 - +0.4
3,915,288 - -0.6
3,915,426 + +0.2
3,915,426 + +0.1

Or see this region's nucleotide sequence