Experiment: L-Leucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3380 and Pf6N2E2_3381 are separated by 150 nucleotides Pf6N2E2_3381 and Pf6N2E2_3382 overlap by 4 nucleotides Pf6N2E2_3382 and Pf6N2E2_3383 are separated by 167 nucleotides Pf6N2E2_3383 and Pf6N2E2_3384 overlap by 4 nucleotides
Pf6N2E2_3380 - L-lactate permease, at 3,910,361 to 3,912,058
_3380
Pf6N2E2_3381 - Predicted L-lactate dehydrogenase, Fe-S oxidoreductase subunit YkgE, at 3,912,209 to 3,913,033
_3381
Pf6N2E2_3382 - Predicted L-lactate dehydrogenase, Iron-sulfur cluster-binding subunit YkgF, at 3,913,030 to 3,913,593
_3382
Pf6N2E2_3383 - Predicted L-lactate dehydrogenase, Iron-sulfur cluster-binding subunit YkgF, at 3,913,761 to 3,914,489
_3383
Pf6N2E2_3384 - Predicted L-lactate dehydrogenase, hypothetical protein subunit YkgG, at 3,914,486 to 3,915,157
_3384
Position (kb)
3913
3914 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3912.056 kb on + strand at 3912.056 kb on + strand at 3912.056 kb on + strand at 3912.056 kb on + strand at 3912.056 kb on + strand at 3912.057 kb on - strand at 3912.057 kb on - strand at 3912.057 kb on - strand at 3912.057 kb on - strand at 3912.063 kb on + strand at 3912.064 kb on - strand at 3912.112 kb on + strand at 3912.112 kb on + strand at 3912.113 kb on - strand at 3912.113 kb on - strand at 3912.155 kb on + strand at 3912.155 kb on + strand at 3912.155 kb on + strand at 3912.155 kb on + strand at 3912.155 kb on + strand at 3912.155 kb on + strand at 3912.156 kb on - strand at 3912.156 kb on - strand at 3912.156 kb on - strand at 3912.157 kb on + strand at 3912.157 kb on + strand at 3912.158 kb on - strand at 3912.158 kb on - strand at 3912.158 kb on - strand at 3912.158 kb on - strand at 3912.225 kb on - strand at 3912.225 kb on - strand at 3912.226 kb on + strand at 3912.282 kb on - strand at 3912.309 kb on - strand, within Pf6N2E2_3381 at 3912.341 kb on + strand, within Pf6N2E2_3381 at 3912.342 kb on - strand, within Pf6N2E2_3381 at 3912.437 kb on + strand, within Pf6N2E2_3381 at 3912.437 kb on + strand, within Pf6N2E2_3381 at 3912.438 kb on - strand, within Pf6N2E2_3381 at 3912.438 kb on - strand, within Pf6N2E2_3381 at 3912.450 kb on - strand, within Pf6N2E2_3381 at 3912.557 kb on + strand, within Pf6N2E2_3381 at 3912.557 kb on + strand, within Pf6N2E2_3381 at 3912.623 kb on + strand, within Pf6N2E2_3381 at 3912.623 kb on + strand, within Pf6N2E2_3381 at 3912.623 kb on + strand, within Pf6N2E2_3381 at 3912.623 kb on + strand, within Pf6N2E2_3381 at 3912.624 kb on - strand, within Pf6N2E2_3381 at 3912.624 kb on - strand, within Pf6N2E2_3381 at 3912.624 kb on - strand, within Pf6N2E2_3381 at 3912.624 kb on - strand, within Pf6N2E2_3381 at 3912.624 kb on - strand, within Pf6N2E2_3381 at 3912.624 kb on - strand, within Pf6N2E2_3381 at 3912.624 kb on - strand, within Pf6N2E2_3381 at 3913.469 kb on + strand, within Pf6N2E2_3382 at 3913.469 kb on + strand, within Pf6N2E2_3382 at 3913.469 kb on + strand, within Pf6N2E2_3382 at 3913.469 kb on + strand, within Pf6N2E2_3382 at 3913.469 kb on + strand, within Pf6N2E2_3382 at 3913.470 kb on - strand, within Pf6N2E2_3382 at 3913.470 kb on - strand, within Pf6N2E2_3382 at 3913.470 kb on - strand, within Pf6N2E2_3382 at 3913.470 kb on - strand, within Pf6N2E2_3382 at 3913.470 kb on - strand, within Pf6N2E2_3382 at 3913.510 kb on + strand, within Pf6N2E2_3382 at 3913.510 kb on + strand, within Pf6N2E2_3382 at 3913.510 kb on + strand, within Pf6N2E2_3382 at 3913.510 kb on + strand, within Pf6N2E2_3382 at 3913.510 kb on + strand, within Pf6N2E2_3382 at 3913.510 kb on + strand, within Pf6N2E2_3382 at 3913.510 kb on + strand, within Pf6N2E2_3382 at 3913.511 kb on - strand, within Pf6N2E2_3382 at 3913.511 kb on - strand, within Pf6N2E2_3382 at 3913.511 kb on - strand, within Pf6N2E2_3382 at 3913.578 kb on - strand at 3913.578 kb on - strand at 3913.620 kb on + strand at 3913.620 kb on + strand at 3913.620 kb on + strand at 3913.621 kb on - strand at 3913.621 kb on - strand at 3913.621 kb on - strand at 3913.857 kb on + strand, within Pf6N2E2_3383 at 3913.857 kb on + strand, within Pf6N2E2_3383 at 3913.858 kb on - strand, within Pf6N2E2_3383 at 3913.858 kb on - strand, within Pf6N2E2_3383 at 3913.858 kb on - strand, within Pf6N2E2_3383 at 3914.006 kb on + strand, within Pf6N2E2_3383 at 3914.006 kb on + strand, within Pf6N2E2_3383 at 3914.007 kb on - strand, within Pf6N2E2_3383 at 3914.007 kb on - strand, within Pf6N2E2_3383 at 3914.025 kb on + strand at 3914.025 kb on + strand, within Pf6N2E2_3383 at 3914.026 kb on - strand, within Pf6N2E2_3383 at 3914.026 kb on - strand, within Pf6N2E2_3383 at 3914.062 kb on - strand, within Pf6N2E2_3383 at 3914.073 kb on - strand, within Pf6N2E2_3383 at 3914.271 kb on + strand, within Pf6N2E2_3383 at 3914.272 kb on - strand, within Pf6N2E2_3383 at 3914.572 kb on - strand, within Pf6N2E2_3384 at 3914.572 kb on - strand, within Pf6N2E2_3384 at 3914.585 kb on + strand, within Pf6N2E2_3384
Per-strain Table
Position Strand Gene LocusTag Fraction L-Leucine (C) remove 3,912,056 + +0.2 3,912,056 + +0.6 3,912,056 + -0.4 3,912,056 + -0.8 3,912,056 + -2.2 3,912,057 - +0.3 3,912,057 - -0.2 3,912,057 - +2.1 3,912,057 - +0.0 3,912,063 + -0.3 3,912,064 - -0.4 3,912,112 + -0.8 3,912,112 + -0.5 3,912,113 - +1.1 3,912,113 - -0.8 3,912,155 + -0.1 3,912,155 + +0.1 3,912,155 + +0.1 3,912,155 + +0.1 3,912,155 + +0.2 3,912,155 + +0.1 3,912,156 - +0.4 3,912,156 - -0.0 3,912,156 - -0.4 3,912,157 + +0.3 3,912,157 + -0.7 3,912,158 - -0.5 3,912,158 - +0.7 3,912,158 - -0.3 3,912,158 - +0.4 3,912,225 - -2.1 3,912,225 - +0.7 3,912,226 + +0.1 3,912,282 - +0.1 3,912,309 - Pf6N2E2_3381 0.12 +0.1 3,912,341 + Pf6N2E2_3381 0.16 -0.1 3,912,342 - Pf6N2E2_3381 0.16 +0.0 3,912,437 + Pf6N2E2_3381 0.28 +0.1 3,912,437 + Pf6N2E2_3381 0.28 -1.0 3,912,438 - Pf6N2E2_3381 0.28 +0.3 3,912,438 - Pf6N2E2_3381 0.28 +0.5 3,912,450 - Pf6N2E2_3381 0.29 +1.0 3,912,557 + Pf6N2E2_3381 0.42 -0.0 3,912,557 + Pf6N2E2_3381 0.42 +0.3 3,912,623 + Pf6N2E2_3381 0.50 +0.1 3,912,623 + Pf6N2E2_3381 0.50 +0.3 3,912,623 + Pf6N2E2_3381 0.50 +0.1 3,912,623 + Pf6N2E2_3381 0.50 -0.5 3,912,624 - Pf6N2E2_3381 0.50 +0.4 3,912,624 - Pf6N2E2_3381 0.50 +0.2 3,912,624 - Pf6N2E2_3381 0.50 +0.6 3,912,624 - Pf6N2E2_3381 0.50 +2.2 3,912,624 - Pf6N2E2_3381 0.50 +0.6 3,912,624 - Pf6N2E2_3381 0.50 +0.2 3,912,624 - Pf6N2E2_3381 0.50 +0.0 3,913,469 + Pf6N2E2_3382 0.78 -3.0 3,913,469 + Pf6N2E2_3382 0.78 -0.8 3,913,469 + Pf6N2E2_3382 0.78 -0.9 3,913,469 + Pf6N2E2_3382 0.78 -0.1 3,913,469 + Pf6N2E2_3382 0.78 -1.2 3,913,470 - Pf6N2E2_3382 0.78 -0.9 3,913,470 - Pf6N2E2_3382 0.78 +0.4 3,913,470 - Pf6N2E2_3382 0.78 +1.0 3,913,470 - Pf6N2E2_3382 0.78 +0.4 3,913,470 - Pf6N2E2_3382 0.78 +0.5 3,913,510 + Pf6N2E2_3382 0.85 -2.1 3,913,510 + Pf6N2E2_3382 0.85 -0.3 3,913,510 + Pf6N2E2_3382 0.85 +0.4 3,913,510 + Pf6N2E2_3382 0.85 -0.3 3,913,510 + Pf6N2E2_3382 0.85 -0.0 3,913,510 + Pf6N2E2_3382 0.85 -0.4 3,913,510 + Pf6N2E2_3382 0.85 +1.3 3,913,511 - Pf6N2E2_3382 0.85 -0.6 3,913,511 - Pf6N2E2_3382 0.85 -0.2 3,913,511 - Pf6N2E2_3382 0.85 +0.0 3,913,578 - +0.3 3,913,578 - -0.7 3,913,620 + +1.3 3,913,620 + +0.7 3,913,620 + -1.3 3,913,621 - +0.5 3,913,621 - -0.2 3,913,621 - -0.2 3,913,857 + Pf6N2E2_3383 0.13 +0.6 3,913,857 + Pf6N2E2_3383 0.13 +0.4 3,913,858 - Pf6N2E2_3383 0.13 -1.7 3,913,858 - Pf6N2E2_3383 0.13 +1.2 3,913,858 - Pf6N2E2_3383 0.13 +0.2 3,914,006 + Pf6N2E2_3383 0.34 -0.1 3,914,006 + Pf6N2E2_3383 0.34 -0.0 3,914,007 - Pf6N2E2_3383 0.34 -0.8 3,914,007 - Pf6N2E2_3383 0.34 +0.3 3,914,025 + +1.4 3,914,025 + Pf6N2E2_3383 0.36 -0.1 3,914,026 - Pf6N2E2_3383 0.36 +1.2 3,914,026 - Pf6N2E2_3383 0.36 +0.7 3,914,062 - Pf6N2E2_3383 0.41 +0.9 3,914,073 - Pf6N2E2_3383 0.43 +0.2 3,914,271 + Pf6N2E2_3383 0.70 +0.5 3,914,272 - Pf6N2E2_3383 0.70 +2.2 3,914,572 - Pf6N2E2_3384 0.13 -0.5 3,914,572 - Pf6N2E2_3384 0.13 -0.0 3,914,585 + Pf6N2E2_3384 0.15 -0.9
Or see this region's nucleotide sequence