Experiment: L-Leucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2408 and Pf6N2E2_2409 are separated by 170 nucleotides Pf6N2E2_2409 and Pf6N2E2_2410 are separated by 33 nucleotides
Pf6N2E2_2408 - 3-hydroxy-3isohexenylglutaryl-CoA:acetate lyase, at 2,858,603 to 2,860,408
_2408
Pf6N2E2_2409 - Methylcrotonyl-CoA carboxylase carboxyl transferase subunit (EC 6.4.1.4), at 2,860,579 to 2,862,195
_2409
Pf6N2E2_2410 - Citronellyl-CoA dehydrogenase, at 2,862,229 to 2,863,386
_2410
Position (kb)
2860
2861
2862
2863 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2859.713 kb on + strand, within Pf6N2E2_2408 at 2859.713 kb on + strand, within Pf6N2E2_2408 at 2859.714 kb on - strand, within Pf6N2E2_2408 at 2859.714 kb on - strand, within Pf6N2E2_2408 at 2860.136 kb on + strand, within Pf6N2E2_2408 at 2860.137 kb on - strand, within Pf6N2E2_2408 at 2860.232 kb on + strand at 2860.233 kb on - strand at 2860.233 kb on - strand at 2860.874 kb on - strand, within Pf6N2E2_2409 at 2860.951 kb on + strand, within Pf6N2E2_2409 at 2860.951 kb on + strand, within Pf6N2E2_2409 at 2860.951 kb on + strand, within Pf6N2E2_2409 at 2860.951 kb on + strand, within Pf6N2E2_2409 at 2860.951 kb on + strand, within Pf6N2E2_2409 at 2860.951 kb on + strand at 2860.952 kb on - strand, within Pf6N2E2_2409 at 2860.952 kb on - strand, within Pf6N2E2_2409 at 2860.952 kb on - strand, within Pf6N2E2_2409 at 2860.952 kb on - strand, within Pf6N2E2_2409 at 2861.038 kb on + strand, within Pf6N2E2_2409 at 2861.039 kb on - strand, within Pf6N2E2_2409 at 2861.052 kb on - strand, within Pf6N2E2_2409 at 2861.052 kb on - strand, within Pf6N2E2_2409 at 2861.052 kb on - strand, within Pf6N2E2_2409 at 2861.156 kb on - strand, within Pf6N2E2_2409 at 2861.314 kb on + strand, within Pf6N2E2_2409 at 2861.314 kb on + strand, within Pf6N2E2_2409 at 2861.315 kb on - strand, within Pf6N2E2_2409 at 2861.429 kb on - strand, within Pf6N2E2_2409 at 2861.444 kb on + strand, within Pf6N2E2_2409 at 2861.444 kb on + strand, within Pf6N2E2_2409 at 2861.479 kb on + strand, within Pf6N2E2_2409 at 2861.480 kb on - strand, within Pf6N2E2_2409 at 2861.480 kb on - strand, within Pf6N2E2_2409 at 2861.480 kb on - strand, within Pf6N2E2_2409 at 2861.842 kb on + strand, within Pf6N2E2_2409 at 2861.842 kb on + strand, within Pf6N2E2_2409 at 2861.842 kb on + strand, within Pf6N2E2_2409 at 2862.056 kb on - strand at 2862.121 kb on + strand at 2862.301 kb on + strand at 2862.302 kb on - strand at 2862.302 kb on - strand at 2862.421 kb on + strand, within Pf6N2E2_2410 at 2862.422 kb on - strand, within Pf6N2E2_2410 at 2862.422 kb on - strand, within Pf6N2E2_2410 at 2862.422 kb on - strand, within Pf6N2E2_2410 at 2862.422 kb on - strand, within Pf6N2E2_2410 at 2862.427 kb on + strand, within Pf6N2E2_2410 at 2862.427 kb on + strand, within Pf6N2E2_2410 at 2862.428 kb on - strand, within Pf6N2E2_2410 at 2862.670 kb on + strand, within Pf6N2E2_2410 at 2862.670 kb on + strand, within Pf6N2E2_2410 at 2862.670 kb on + strand, within Pf6N2E2_2410 at 2862.671 kb on - strand, within Pf6N2E2_2410 at 2862.671 kb on - strand, within Pf6N2E2_2410 at 2862.671 kb on - strand, within Pf6N2E2_2410 at 2862.671 kb on - strand, within Pf6N2E2_2410 at 2862.671 kb on - strand, within Pf6N2E2_2410 at 2862.741 kb on + strand, within Pf6N2E2_2410 at 2862.742 kb on - strand, within Pf6N2E2_2410 at 2862.742 kb on - strand, within Pf6N2E2_2410 at 2862.742 kb on - strand, within Pf6N2E2_2410 at 2863.018 kb on + strand, within Pf6N2E2_2410 at 2863.019 kb on - strand, within Pf6N2E2_2410 at 2863.144 kb on + strand, within Pf6N2E2_2410 at 2863.145 kb on - strand, within Pf6N2E2_2410 at 2863.145 kb on - strand, within Pf6N2E2_2410 at 2863.145 kb on - strand, within Pf6N2E2_2410 at 2863.145 kb on - strand, within Pf6N2E2_2410
Per-strain Table
Position Strand Gene LocusTag Fraction L-Leucine (C) remove 2,859,713 + Pf6N2E2_2408 0.61 +0.2 2,859,713 + Pf6N2E2_2408 0.61 -0.1 2,859,714 - Pf6N2E2_2408 0.62 +0.1 2,859,714 - Pf6N2E2_2408 0.62 -0.3 2,860,136 + Pf6N2E2_2408 0.85 -0.6 2,860,137 - Pf6N2E2_2408 0.85 +1.3 2,860,232 + -0.6 2,860,233 - -0.7 2,860,233 - -0.3 2,860,874 - Pf6N2E2_2409 0.18 +0.0 2,860,951 + Pf6N2E2_2409 0.23 -0.6 2,860,951 + Pf6N2E2_2409 0.23 +1.1 2,860,951 + Pf6N2E2_2409 0.23 -1.5 2,860,951 + Pf6N2E2_2409 0.23 -0.2 2,860,951 + Pf6N2E2_2409 0.23 +0.2 2,860,951 + +0.9 2,860,952 - Pf6N2E2_2409 0.23 +0.1 2,860,952 - Pf6N2E2_2409 0.23 -0.3 2,860,952 - Pf6N2E2_2409 0.23 -0.1 2,860,952 - Pf6N2E2_2409 0.23 -0.8 2,861,038 + Pf6N2E2_2409 0.28 -0.1 2,861,039 - Pf6N2E2_2409 0.28 -1.2 2,861,052 - Pf6N2E2_2409 0.29 +0.2 2,861,052 - Pf6N2E2_2409 0.29 -0.4 2,861,052 - Pf6N2E2_2409 0.29 -0.1 2,861,156 - Pf6N2E2_2409 0.36 +0.6 2,861,314 + Pf6N2E2_2409 0.45 +0.5 2,861,314 + Pf6N2E2_2409 0.45 +0.1 2,861,315 - Pf6N2E2_2409 0.46 +0.1 2,861,429 - Pf6N2E2_2409 0.53 +0.6 2,861,444 + Pf6N2E2_2409 0.53 -1.8 2,861,444 + Pf6N2E2_2409 0.53 +0.1 2,861,479 + Pf6N2E2_2409 0.56 +0.5 2,861,480 - Pf6N2E2_2409 0.56 -0.1 2,861,480 - Pf6N2E2_2409 0.56 +0.5 2,861,480 - Pf6N2E2_2409 0.56 -0.8 2,861,842 + Pf6N2E2_2409 0.78 +0.1 2,861,842 + Pf6N2E2_2409 0.78 -0.1 2,861,842 + Pf6N2E2_2409 0.78 +0.4 2,862,056 - -0.2 2,862,121 + -0.2 2,862,301 + +0.8 2,862,302 - +2.3 2,862,302 - -0.2 2,862,421 + Pf6N2E2_2410 0.17 +0.2 2,862,422 - Pf6N2E2_2410 0.17 +0.3 2,862,422 - Pf6N2E2_2410 0.17 +0.7 2,862,422 - Pf6N2E2_2410 0.17 -0.5 2,862,422 - Pf6N2E2_2410 0.17 +1.8 2,862,427 + Pf6N2E2_2410 0.17 +0.3 2,862,427 + Pf6N2E2_2410 0.17 -0.6 2,862,428 - Pf6N2E2_2410 0.17 -0.8 2,862,670 + Pf6N2E2_2410 0.38 -0.8 2,862,670 + Pf6N2E2_2410 0.38 +0.7 2,862,670 + Pf6N2E2_2410 0.38 +0.1 2,862,671 - Pf6N2E2_2410 0.38 +0.1 2,862,671 - Pf6N2E2_2410 0.38 +0.1 2,862,671 - Pf6N2E2_2410 0.38 +0.9 2,862,671 - Pf6N2E2_2410 0.38 +0.5 2,862,671 - Pf6N2E2_2410 0.38 -0.6 2,862,741 + Pf6N2E2_2410 0.44 +0.9 2,862,742 - Pf6N2E2_2410 0.44 -0.6 2,862,742 - Pf6N2E2_2410 0.44 -0.3 2,862,742 - Pf6N2E2_2410 0.44 -0.7 2,863,018 + Pf6N2E2_2410 0.68 +0.9 2,863,019 - Pf6N2E2_2410 0.68 +0.5 2,863,144 + Pf6N2E2_2410 0.79 +0.4 2,863,145 - Pf6N2E2_2410 0.79 +0.7 2,863,145 - Pf6N2E2_2410 0.79 -1.3 2,863,145 - Pf6N2E2_2410 0.79 +0.1 2,863,145 - Pf6N2E2_2410 0.79 +0.0
Or see this region's nucleotide sequence