Experiment: L-Leucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2192 and Pf6N2E2_2193 are separated by 13 nucleotides Pf6N2E2_2193 and Pf6N2E2_2194 overlap by 4 nucleotides Pf6N2E2_2194 and Pf6N2E2_2195 are separated by 29 nucleotides Pf6N2E2_2195 and Pf6N2E2_2196 are separated by 122 nucleotides
Pf6N2E2_2192 - 3-methylcrotonyl-CoA carboxylase, beta subunit (EC 6.4.1.4) (from data) , at 2,597,887 to 2,599,494
_2192
Pf6N2E2_2193 - Methylglutaconyl-CoA hydratase (EC 4.2.1.18) (from data) , at 2,599,508 to 2,600,326
_2193
Pf6N2E2_2194 - 3-methylcrotonyl-CoA carboxylase, alpha (biotin-containing) subunit (EC 6.4.1.4) (from data) , at 2,600,323 to 2,602,272
_2194
Pf6N2E2_2195 - cI, at 2,602,302 to 2,602,958
_2195
Pf6N2E2_2196 - FIG00953347: hypothetical protein, at 2,603,081 to 2,603,422
_2196
Position (kb)
2600
2601
2602
2603 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2599.339 kb on + strand at 2599.340 kb on - strand at 2599.340 kb on - strand at 2599.360 kb on + strand at 2599.361 kb on - strand at 2599.361 kb on - strand at 2599.361 kb on - strand at 2599.363 kb on + strand at 2599.363 kb on + strand at 2599.363 kb on + strand at 2599.363 kb on + strand at 2599.364 kb on - strand at 2599.364 kb on - strand at 2599.493 kb on - strand at 2599.513 kb on + strand at 2599.745 kb on + strand, within Pf6N2E2_2193 at 2599.790 kb on + strand, within Pf6N2E2_2193 at 2599.790 kb on + strand, within Pf6N2E2_2193 at 2599.790 kb on + strand, within Pf6N2E2_2193 at 2599.790 kb on + strand, within Pf6N2E2_2193 at 2599.790 kb on + strand, within Pf6N2E2_2193 at 2599.791 kb on - strand, within Pf6N2E2_2193 at 2599.791 kb on - strand, within Pf6N2E2_2193 at 2599.791 kb on - strand, within Pf6N2E2_2193 at 2599.791 kb on - strand, within Pf6N2E2_2193 at 2599.791 kb on - strand, within Pf6N2E2_2193 at 2599.864 kb on + strand, within Pf6N2E2_2193 at 2599.997 kb on + strand, within Pf6N2E2_2193 at 2599.998 kb on - strand, within Pf6N2E2_2193 at 2600.201 kb on + strand, within Pf6N2E2_2193 at 2600.512 kb on + strand at 2600.512 kb on + strand at 2600.513 kb on - strand at 2600.513 kb on - strand at 2600.513 kb on - strand at 2600.972 kb on + strand, within Pf6N2E2_2194 at 2600.973 kb on - strand, within Pf6N2E2_2194 at 2600.973 kb on - strand, within Pf6N2E2_2194 at 2601.136 kb on + strand, within Pf6N2E2_2194 at 2601.137 kb on - strand, within Pf6N2E2_2194 at 2601.352 kb on + strand, within Pf6N2E2_2194 at 2601.353 kb on - strand, within Pf6N2E2_2194 at 2601.353 kb on - strand, within Pf6N2E2_2194 at 2601.406 kb on + strand, within Pf6N2E2_2194 at 2601.481 kb on + strand, within Pf6N2E2_2194 at 2601.482 kb on - strand, within Pf6N2E2_2194 at 2601.552 kb on + strand, within Pf6N2E2_2194 at 2601.552 kb on + strand, within Pf6N2E2_2194 at 2601.552 kb on + strand, within Pf6N2E2_2194 at 2601.552 kb on + strand, within Pf6N2E2_2194 at 2601.553 kb on - strand, within Pf6N2E2_2194 at 2601.553 kb on - strand, within Pf6N2E2_2194 at 2601.553 kb on - strand, within Pf6N2E2_2194 at 2601.611 kb on + strand, within Pf6N2E2_2194 at 2601.761 kb on + strand, within Pf6N2E2_2194 at 2601.761 kb on + strand, within Pf6N2E2_2194 at 2601.761 kb on + strand, within Pf6N2E2_2194 at 2601.762 kb on - strand, within Pf6N2E2_2194 at 2601.762 kb on - strand, within Pf6N2E2_2194 at 2601.762 kb on - strand, within Pf6N2E2_2194 at 2601.979 kb on + strand, within Pf6N2E2_2194 at 2601.979 kb on + strand, within Pf6N2E2_2194 at 2601.979 kb on + strand, within Pf6N2E2_2194 at 2601.980 kb on - strand, within Pf6N2E2_2194 at 2602.089 kb on + strand at 2602.090 kb on - strand at 2602.090 kb on - strand at 2602.213 kb on + strand at 2602.213 kb on + strand at 2602.214 kb on - strand at 2602.270 kb on + strand at 2602.270 kb on + strand at 2602.357 kb on + strand at 2602.357 kb on + strand at 2602.358 kb on - strand at 2602.397 kb on - strand, within Pf6N2E2_2195 at 2602.627 kb on + strand, within Pf6N2E2_2195 at 2602.628 kb on - strand, within Pf6N2E2_2195 at 2602.703 kb on - strand, within Pf6N2E2_2195 at 2602.936 kb on - strand at 2602.943 kb on + strand at 2602.944 kb on - strand at 2602.944 kb on - strand at 2602.961 kb on + strand at 2602.962 kb on - strand at 2602.962 kb on - strand at 2603.086 kb on + strand at 2603.086 kb on + strand at 2603.087 kb on - strand at 2603.175 kb on - strand, within Pf6N2E2_2196 at 2603.175 kb on - strand, within Pf6N2E2_2196 at 2603.175 kb on - strand, within Pf6N2E2_2196 at 2603.177 kb on + strand, within Pf6N2E2_2196 at 2603.177 kb on + strand, within Pf6N2E2_2196 at 2603.177 kb on + strand, within Pf6N2E2_2196 at 2603.178 kb on - strand, within Pf6N2E2_2196 at 2603.178 kb on - strand, within Pf6N2E2_2196
Per-strain Table
Position Strand Gene LocusTag Fraction L-Leucine (C) remove 2,599,339 + -1.9 2,599,340 - -3.4 2,599,340 - -0.8 2,599,360 + -0.2 2,599,361 - -3.1 2,599,361 - -2.6 2,599,361 - -3.5 2,599,363 + -3.5 2,599,363 + -3.8 2,599,363 + -2.9 2,599,363 + -3.0 2,599,364 - -1.5 2,599,364 - -1.8 2,599,493 - -4.7 2,599,513 + -2.9 2,599,745 + Pf6N2E2_2193 0.29 -1.6 2,599,790 + Pf6N2E2_2193 0.34 -4.0 2,599,790 + Pf6N2E2_2193 0.34 -2.2 2,599,790 + Pf6N2E2_2193 0.34 -3.1 2,599,790 + Pf6N2E2_2193 0.34 -0.5 2,599,790 + Pf6N2E2_2193 0.34 -2.9 2,599,791 - Pf6N2E2_2193 0.35 -3.6 2,599,791 - Pf6N2E2_2193 0.35 -2.9 2,599,791 - Pf6N2E2_2193 0.35 -4.2 2,599,791 - Pf6N2E2_2193 0.35 -4.3 2,599,791 - Pf6N2E2_2193 0.35 -2.3 2,599,864 + Pf6N2E2_2193 0.43 -2.6 2,599,997 + Pf6N2E2_2193 0.60 -3.1 2,599,998 - Pf6N2E2_2193 0.60 -3.4 2,600,201 + Pf6N2E2_2193 0.85 -1.6 2,600,512 + -4.1 2,600,512 + -4.2 2,600,513 - -2.5 2,600,513 - -2.8 2,600,513 - -3.1 2,600,972 + Pf6N2E2_2194 0.33 -2.2 2,600,973 - Pf6N2E2_2194 0.33 -2.4 2,600,973 - Pf6N2E2_2194 0.33 -3.9 2,601,136 + Pf6N2E2_2194 0.42 -3.8 2,601,137 - Pf6N2E2_2194 0.42 -4.2 2,601,352 + Pf6N2E2_2194 0.53 -3.4 2,601,353 - Pf6N2E2_2194 0.53 -2.8 2,601,353 - Pf6N2E2_2194 0.53 -2.9 2,601,406 + Pf6N2E2_2194 0.56 -2.8 2,601,481 + Pf6N2E2_2194 0.59 -3.3 2,601,482 - Pf6N2E2_2194 0.59 -4.0 2,601,552 + Pf6N2E2_2194 0.63 -3.8 2,601,552 + Pf6N2E2_2194 0.63 -2.6 2,601,552 + Pf6N2E2_2194 0.63 -3.6 2,601,552 + Pf6N2E2_2194 0.63 -3.0 2,601,553 - Pf6N2E2_2194 0.63 -3.4 2,601,553 - Pf6N2E2_2194 0.63 -3.0 2,601,553 - Pf6N2E2_2194 0.63 -3.1 2,601,611 + Pf6N2E2_2194 0.66 -2.2 2,601,761 + Pf6N2E2_2194 0.74 -3.9 2,601,761 + Pf6N2E2_2194 0.74 -3.4 2,601,761 + Pf6N2E2_2194 0.74 -3.6 2,601,762 - Pf6N2E2_2194 0.74 -3.1 2,601,762 - Pf6N2E2_2194 0.74 -3.4 2,601,762 - Pf6N2E2_2194 0.74 -3.4 2,601,979 + Pf6N2E2_2194 0.85 -2.9 2,601,979 + Pf6N2E2_2194 0.85 -4.5 2,601,979 + Pf6N2E2_2194 0.85 -3.2 2,601,980 - Pf6N2E2_2194 0.85 -3.0 2,602,089 + -1.8 2,602,090 - -2.8 2,602,090 - -2.6 2,602,213 + -4.4 2,602,213 + -3.9 2,602,214 - -4.4 2,602,270 + -1.9 2,602,270 + +0.4 2,602,357 + -0.0 2,602,357 + -0.7 2,602,358 - +0.3 2,602,397 - Pf6N2E2_2195 0.14 +0.3 2,602,627 + Pf6N2E2_2195 0.49 +1.0 2,602,628 - Pf6N2E2_2195 0.50 -0.0 2,602,703 - Pf6N2E2_2195 0.61 +0.5 2,602,936 - +0.3 2,602,943 + +0.7 2,602,944 - -0.0 2,602,944 - +0.2 2,602,961 + -0.5 2,602,962 - -0.4 2,602,962 - -0.3 2,603,086 + +0.6 2,603,086 + -0.1 2,603,087 - -1.8 2,603,175 - Pf6N2E2_2196 0.27 +0.4 2,603,175 - Pf6N2E2_2196 0.27 -0.8 2,603,175 - Pf6N2E2_2196 0.27 +0.2 2,603,177 + Pf6N2E2_2196 0.28 +2.0 2,603,177 + Pf6N2E2_2196 0.28 +0.4 2,603,177 + Pf6N2E2_2196 0.28 -0.0 2,603,178 - Pf6N2E2_2196 0.28 -1.0 2,603,178 - Pf6N2E2_2196 0.28 +0.5
Or see this region's nucleotide sequence