Experiment: L-Leucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2192 and Pf6N2E2_2193 are separated by 13 nucleotides Pf6N2E2_2193 and Pf6N2E2_2194 overlap by 4 nucleotides
Pf6N2E2_2192 - 3-methylcrotonyl-CoA carboxylase, beta subunit (EC 6.4.1.4) (from data) , at 2,597,887 to 2,599,494
_2192
Pf6N2E2_2193 - Methylglutaconyl-CoA hydratase (EC 4.2.1.18) (from data) , at 2,599,508 to 2,600,326
_2193
Pf6N2E2_2194 - 3-methylcrotonyl-CoA carboxylase, alpha (biotin-containing) subunit (EC 6.4.1.4) (from data) , at 2,600,323 to 2,602,272
_2194
Position (kb)
2599
2600
2601 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2598.628 kb on + strand, within Pf6N2E2_2192 at 2598.628 kb on + strand, within Pf6N2E2_2192 at 2598.629 kb on - strand, within Pf6N2E2_2192 at 2598.755 kb on - strand, within Pf6N2E2_2192 at 2599.005 kb on - strand, within Pf6N2E2_2192 at 2599.039 kb on + strand, within Pf6N2E2_2192 at 2599.039 kb on + strand, within Pf6N2E2_2192 at 2599.040 kb on - strand, within Pf6N2E2_2192 at 2599.150 kb on + strand, within Pf6N2E2_2192 at 2599.150 kb on + strand, within Pf6N2E2_2192 at 2599.151 kb on - strand, within Pf6N2E2_2192 at 2599.339 kb on + strand at 2599.340 kb on - strand at 2599.340 kb on - strand at 2599.360 kb on + strand at 2599.361 kb on - strand at 2599.361 kb on - strand at 2599.361 kb on - strand at 2599.363 kb on + strand at 2599.363 kb on + strand at 2599.363 kb on + strand at 2599.363 kb on + strand at 2599.364 kb on - strand at 2599.364 kb on - strand at 2599.493 kb on - strand at 2599.513 kb on + strand at 2599.745 kb on + strand, within Pf6N2E2_2193 at 2599.790 kb on + strand, within Pf6N2E2_2193 at 2599.790 kb on + strand, within Pf6N2E2_2193 at 2599.790 kb on + strand, within Pf6N2E2_2193 at 2599.790 kb on + strand, within Pf6N2E2_2193 at 2599.790 kb on + strand, within Pf6N2E2_2193 at 2599.791 kb on - strand, within Pf6N2E2_2193 at 2599.791 kb on - strand, within Pf6N2E2_2193 at 2599.791 kb on - strand, within Pf6N2E2_2193 at 2599.791 kb on - strand, within Pf6N2E2_2193 at 2599.791 kb on - strand, within Pf6N2E2_2193 at 2599.864 kb on + strand, within Pf6N2E2_2193 at 2599.997 kb on + strand, within Pf6N2E2_2193 at 2599.998 kb on - strand, within Pf6N2E2_2193 at 2600.201 kb on + strand, within Pf6N2E2_2193 at 2600.512 kb on + strand at 2600.512 kb on + strand at 2600.513 kb on - strand at 2600.513 kb on - strand at 2600.513 kb on - strand at 2600.972 kb on + strand, within Pf6N2E2_2194 at 2600.973 kb on - strand, within Pf6N2E2_2194 at 2600.973 kb on - strand, within Pf6N2E2_2194 at 2601.136 kb on + strand, within Pf6N2E2_2194 at 2601.137 kb on - strand, within Pf6N2E2_2194
Per-strain Table
Position Strand Gene LocusTag Fraction L-Leucine (C) remove 2,598,628 + Pf6N2E2_2192 0.46 -2.5 2,598,628 + Pf6N2E2_2192 0.46 -3.9 2,598,629 - Pf6N2E2_2192 0.46 -3.7 2,598,755 - Pf6N2E2_2192 0.54 -3.2 2,599,005 - Pf6N2E2_2192 0.70 -2.5 2,599,039 + Pf6N2E2_2192 0.72 -3.4 2,599,039 + Pf6N2E2_2192 0.72 -0.8 2,599,040 - Pf6N2E2_2192 0.72 -0.2 2,599,150 + Pf6N2E2_2192 0.79 -2.4 2,599,150 + Pf6N2E2_2192 0.79 -1.2 2,599,151 - Pf6N2E2_2192 0.79 -3.6 2,599,339 + -1.9 2,599,340 - -3.4 2,599,340 - -0.8 2,599,360 + -0.2 2,599,361 - -3.1 2,599,361 - -2.6 2,599,361 - -3.5 2,599,363 + -3.5 2,599,363 + -3.8 2,599,363 + -2.9 2,599,363 + -3.0 2,599,364 - -1.5 2,599,364 - -1.8 2,599,493 - -4.7 2,599,513 + -2.9 2,599,745 + Pf6N2E2_2193 0.29 -1.6 2,599,790 + Pf6N2E2_2193 0.34 -4.0 2,599,790 + Pf6N2E2_2193 0.34 -2.2 2,599,790 + Pf6N2E2_2193 0.34 -3.1 2,599,790 + Pf6N2E2_2193 0.34 -0.5 2,599,790 + Pf6N2E2_2193 0.34 -2.9 2,599,791 - Pf6N2E2_2193 0.35 -3.6 2,599,791 - Pf6N2E2_2193 0.35 -2.9 2,599,791 - Pf6N2E2_2193 0.35 -4.2 2,599,791 - Pf6N2E2_2193 0.35 -4.3 2,599,791 - Pf6N2E2_2193 0.35 -2.3 2,599,864 + Pf6N2E2_2193 0.43 -2.6 2,599,997 + Pf6N2E2_2193 0.60 -3.1 2,599,998 - Pf6N2E2_2193 0.60 -3.4 2,600,201 + Pf6N2E2_2193 0.85 -1.6 2,600,512 + -4.1 2,600,512 + -4.2 2,600,513 - -2.5 2,600,513 - -2.8 2,600,513 - -3.1 2,600,972 + Pf6N2E2_2194 0.33 -2.2 2,600,973 - Pf6N2E2_2194 0.33 -2.4 2,600,973 - Pf6N2E2_2194 0.33 -3.9 2,601,136 + Pf6N2E2_2194 0.42 -3.8 2,601,137 - Pf6N2E2_2194 0.42 -4.2
Or see this region's nucleotide sequence