Experiment: L-Leucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2191 and Pf6N2E2_2192 are separated by 11 nucleotides Pf6N2E2_2192 and Pf6N2E2_2193 are separated by 13 nucleotides Pf6N2E2_2193 and Pf6N2E2_2194 overlap by 4 nucleotides
Pf6N2E2_2191 - Isovaleryl-CoA dehydrogenase (EC 1.3.8.4) (from data) , at 2,596,712 to 2,597,875
_2191
Pf6N2E2_2192 - 3-methylcrotonyl-CoA carboxylase, beta subunit (EC 6.4.1.4) (from data) , at 2,597,887 to 2,599,494
_2192
Pf6N2E2_2193 - Methylglutaconyl-CoA hydratase (EC 4.2.1.18) (from data) , at 2,599,508 to 2,600,326
_2193
Pf6N2E2_2194 - 3-methylcrotonyl-CoA carboxylase, alpha (biotin-containing) subunit (EC 6.4.1.4) (from data) , at 2,600,323 to 2,602,272
_2194
Position (kb)
2597
2598
2599
2600 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 2596.901 kb on + strand, within Pf6N2E2_2191 at 2596.922 kb on + strand, within Pf6N2E2_2191 at 2596.923 kb on - strand, within Pf6N2E2_2191 at 2596.986 kb on - strand, within Pf6N2E2_2191 at 2596.986 kb on - strand, within Pf6N2E2_2191 at 2597.054 kb on + strand, within Pf6N2E2_2191 at 2597.055 kb on - strand, within Pf6N2E2_2191 at 2597.168 kb on + strand, within Pf6N2E2_2191 at 2597.168 kb on + strand, within Pf6N2E2_2191 at 2597.168 kb on + strand, within Pf6N2E2_2191 at 2597.169 kb on - strand, within Pf6N2E2_2191 at 2597.169 kb on - strand, within Pf6N2E2_2191 at 2597.169 kb on - strand, within Pf6N2E2_2191 at 2597.169 kb on - strand, within Pf6N2E2_2191 at 2597.169 kb on - strand, within Pf6N2E2_2191 at 2597.222 kb on + strand, within Pf6N2E2_2191 at 2597.222 kb on + strand, within Pf6N2E2_2191 at 2597.223 kb on - strand, within Pf6N2E2_2191 at 2597.223 kb on - strand, within Pf6N2E2_2191 at 2597.232 kb on - strand, within Pf6N2E2_2191 at 2597.232 kb on - strand, within Pf6N2E2_2191 at 2597.317 kb on + strand, within Pf6N2E2_2191 at 2597.318 kb on - strand, within Pf6N2E2_2191 at 2597.318 kb on - strand, within Pf6N2E2_2191 at 2597.516 kb on + strand, within Pf6N2E2_2191 at 2597.517 kb on - strand, within Pf6N2E2_2191 at 2597.517 kb on - strand, within Pf6N2E2_2191 at 2597.517 kb on - strand, within Pf6N2E2_2191 at 2597.591 kb on + strand, within Pf6N2E2_2191 at 2597.591 kb on + strand, within Pf6N2E2_2191 at 2597.591 kb on + strand, within Pf6N2E2_2191 at 2597.591 kb on + strand, within Pf6N2E2_2191 at 2597.591 kb on + strand, within Pf6N2E2_2191 at 2597.591 kb on + strand, within Pf6N2E2_2191 at 2597.591 kb on + strand, within Pf6N2E2_2191 at 2597.591 kb on + strand, within Pf6N2E2_2191 at 2597.591 kb on + strand, within Pf6N2E2_2191 at 2597.591 kb on + strand, within Pf6N2E2_2191 at 2597.591 kb on + strand, within Pf6N2E2_2191 at 2597.591 kb on + strand, within Pf6N2E2_2191 at 2597.591 kb on + strand, within Pf6N2E2_2191 at 2597.592 kb on - strand, within Pf6N2E2_2191 at 2597.592 kb on - strand, within Pf6N2E2_2191 at 2597.592 kb on - strand, within Pf6N2E2_2191 at 2597.592 kb on - strand, within Pf6N2E2_2191 at 2597.592 kb on - strand, within Pf6N2E2_2191 at 2597.592 kb on - strand, within Pf6N2E2_2191 at 2597.592 kb on - strand, within Pf6N2E2_2191 at 2597.624 kb on + strand, within Pf6N2E2_2191 at 2597.624 kb on + strand, within Pf6N2E2_2191 at 2597.624 kb on + strand, within Pf6N2E2_2191 at 2597.624 kb on + strand, within Pf6N2E2_2191 at 2597.625 kb on - strand, within Pf6N2E2_2191 at 2597.625 kb on - strand, within Pf6N2E2_2191 at 2597.690 kb on + strand, within Pf6N2E2_2191 at 2597.690 kb on + strand, within Pf6N2E2_2191 at 2597.691 kb on - strand, within Pf6N2E2_2191 at 2597.753 kb on + strand, within Pf6N2E2_2191 at 2597.753 kb on + strand, within Pf6N2E2_2191 at 2597.753 kb on + strand, within Pf6N2E2_2191 at 2597.753 kb on + strand, within Pf6N2E2_2191 at 2597.754 kb on - strand, within Pf6N2E2_2191 at 2597.754 kb on - strand, within Pf6N2E2_2191 at 2597.754 kb on - strand, within Pf6N2E2_2191 at 2597.754 kb on - strand, within Pf6N2E2_2191 at 2597.802 kb on - strand at 2598.047 kb on + strand at 2598.048 kb on - strand, within Pf6N2E2_2192 at 2598.048 kb on - strand, within Pf6N2E2_2192 at 2598.235 kb on + strand, within Pf6N2E2_2192 at 2598.235 kb on + strand, within Pf6N2E2_2192 at 2598.235 kb on + strand, within Pf6N2E2_2192 at 2598.235 kb on + strand, within Pf6N2E2_2192 at 2598.235 kb on + strand, within Pf6N2E2_2192 at 2598.236 kb on - strand, within Pf6N2E2_2192 at 2598.307 kb on + strand, within Pf6N2E2_2192 at 2598.307 kb on + strand, within Pf6N2E2_2192 at 2598.308 kb on - strand, within Pf6N2E2_2192 at 2598.466 kb on + strand, within Pf6N2E2_2192 at 2598.466 kb on + strand, within Pf6N2E2_2192 at 2598.466 kb on + strand, within Pf6N2E2_2192 at 2598.466 kb on + strand, within Pf6N2E2_2192 at 2598.466 kb on + strand, within Pf6N2E2_2192 at 2598.467 kb on - strand, within Pf6N2E2_2192 at 2598.467 kb on - strand, within Pf6N2E2_2192 at 2598.467 kb on - strand, within Pf6N2E2_2192 at 2598.467 kb on - strand, within Pf6N2E2_2192 at 2598.467 kb on - strand, within Pf6N2E2_2192 at 2598.467 kb on - strand, within Pf6N2E2_2192 at 2598.628 kb on + strand, within Pf6N2E2_2192 at 2598.628 kb on + strand, within Pf6N2E2_2192 at 2598.629 kb on - strand, within Pf6N2E2_2192 at 2598.755 kb on - strand, within Pf6N2E2_2192 at 2599.005 kb on - strand, within Pf6N2E2_2192 at 2599.039 kb on + strand, within Pf6N2E2_2192 at 2599.039 kb on + strand, within Pf6N2E2_2192 at 2599.040 kb on - strand, within Pf6N2E2_2192 at 2599.150 kb on + strand, within Pf6N2E2_2192 at 2599.150 kb on + strand, within Pf6N2E2_2192 at 2599.151 kb on - strand, within Pf6N2E2_2192 at 2599.339 kb on + strand at 2599.340 kb on - strand at 2599.340 kb on - strand at 2599.360 kb on + strand at 2599.361 kb on - strand at 2599.361 kb on - strand at 2599.361 kb on - strand at 2599.363 kb on + strand at 2599.363 kb on + strand at 2599.363 kb on + strand at 2599.363 kb on + strand at 2599.364 kb on - strand at 2599.364 kb on - strand at 2599.493 kb on - strand at 2599.513 kb on + strand at 2599.745 kb on + strand, within Pf6N2E2_2193 at 2599.790 kb on + strand, within Pf6N2E2_2193 at 2599.790 kb on + strand, within Pf6N2E2_2193 at 2599.790 kb on + strand, within Pf6N2E2_2193 at 2599.790 kb on + strand, within Pf6N2E2_2193 at 2599.790 kb on + strand, within Pf6N2E2_2193 at 2599.791 kb on - strand, within Pf6N2E2_2193 at 2599.791 kb on - strand, within Pf6N2E2_2193 at 2599.791 kb on - strand, within Pf6N2E2_2193 at 2599.791 kb on - strand, within Pf6N2E2_2193 at 2599.791 kb on - strand, within Pf6N2E2_2193 at 2599.864 kb on + strand, within Pf6N2E2_2193 at 2599.997 kb on + strand, within Pf6N2E2_2193 at 2599.998 kb on - strand, within Pf6N2E2_2193 at 2600.201 kb on + strand, within Pf6N2E2_2193
Per-strain Table
Position Strand Gene LocusTag Fraction L-Leucine (C) remove 2,596,901 + Pf6N2E2_2191 0.16 -1.5 2,596,922 + Pf6N2E2_2191 0.18 -3.1 2,596,923 - Pf6N2E2_2191 0.18 -3.3 2,596,986 - Pf6N2E2_2191 0.24 -1.5 2,596,986 - Pf6N2E2_2191 0.24 -3.7 2,597,054 + Pf6N2E2_2191 0.29 -1.9 2,597,055 - Pf6N2E2_2191 0.29 -2.5 2,597,168 + Pf6N2E2_2191 0.39 -2.9 2,597,168 + Pf6N2E2_2191 0.39 -1.2 2,597,168 + Pf6N2E2_2191 0.39 -1.8 2,597,169 - Pf6N2E2_2191 0.39 -2.8 2,597,169 - Pf6N2E2_2191 0.39 -2.6 2,597,169 - Pf6N2E2_2191 0.39 -4.2 2,597,169 - Pf6N2E2_2191 0.39 -5.0 2,597,169 - Pf6N2E2_2191 0.39 -1.8 2,597,222 + Pf6N2E2_2191 0.44 -1.5 2,597,222 + Pf6N2E2_2191 0.44 -3.5 2,597,223 - Pf6N2E2_2191 0.44 -2.5 2,597,223 - Pf6N2E2_2191 0.44 -3.3 2,597,232 - Pf6N2E2_2191 0.45 -1.8 2,597,232 - Pf6N2E2_2191 0.45 -2.2 2,597,317 + Pf6N2E2_2191 0.52 -4.0 2,597,318 - Pf6N2E2_2191 0.52 -3.2 2,597,318 - Pf6N2E2_2191 0.52 -2.8 2,597,516 + Pf6N2E2_2191 0.69 -1.8 2,597,517 - Pf6N2E2_2191 0.69 -2.6 2,597,517 - Pf6N2E2_2191 0.69 -3.1 2,597,517 - Pf6N2E2_2191 0.69 -3.8 2,597,591 + Pf6N2E2_2191 0.76 -2.8 2,597,591 + Pf6N2E2_2191 0.76 +0.4 2,597,591 + Pf6N2E2_2191 0.76 -3.5 2,597,591 + Pf6N2E2_2191 0.76 -4.3 2,597,591 + Pf6N2E2_2191 0.76 -1.5 2,597,591 + Pf6N2E2_2191 0.76 -4.4 2,597,591 + Pf6N2E2_2191 0.76 -1.2 2,597,591 + Pf6N2E2_2191 0.76 -3.0 2,597,591 + Pf6N2E2_2191 0.76 -2.5 2,597,591 + Pf6N2E2_2191 0.76 -2.8 2,597,591 + Pf6N2E2_2191 0.76 -0.8 2,597,591 + Pf6N2E2_2191 0.76 -1.5 2,597,591 + Pf6N2E2_2191 0.76 -4.0 2,597,592 - Pf6N2E2_2191 0.76 -3.6 2,597,592 - Pf6N2E2_2191 0.76 -3.4 2,597,592 - Pf6N2E2_2191 0.76 -1.7 2,597,592 - Pf6N2E2_2191 0.76 -3.8 2,597,592 - Pf6N2E2_2191 0.76 -3.2 2,597,592 - Pf6N2E2_2191 0.76 -2.0 2,597,592 - Pf6N2E2_2191 0.76 -2.2 2,597,624 + Pf6N2E2_2191 0.78 -2.5 2,597,624 + Pf6N2E2_2191 0.78 -2.4 2,597,624 + Pf6N2E2_2191 0.78 -3.1 2,597,624 + Pf6N2E2_2191 0.78 -4.5 2,597,625 - Pf6N2E2_2191 0.78 -2.3 2,597,625 - Pf6N2E2_2191 0.78 -2.0 2,597,690 + Pf6N2E2_2191 0.84 -2.0 2,597,690 + Pf6N2E2_2191 0.84 -4.0 2,597,691 - Pf6N2E2_2191 0.84 -3.7 2,597,753 + Pf6N2E2_2191 0.89 -3.0 2,597,753 + Pf6N2E2_2191 0.89 -0.5 2,597,753 + Pf6N2E2_2191 0.89 -5.2 2,597,753 + Pf6N2E2_2191 0.89 -3.0 2,597,754 - Pf6N2E2_2191 0.90 -2.1 2,597,754 - Pf6N2E2_2191 0.90 -4.2 2,597,754 - Pf6N2E2_2191 0.90 -2.4 2,597,754 - Pf6N2E2_2191 0.90 -4.2 2,597,802 - -3.6 2,598,047 + -2.8 2,598,048 - Pf6N2E2_2192 0.10 -2.9 2,598,048 - Pf6N2E2_2192 0.10 -3.1 2,598,235 + Pf6N2E2_2192 0.22 -3.1 2,598,235 + Pf6N2E2_2192 0.22 -3.8 2,598,235 + Pf6N2E2_2192 0.22 -2.8 2,598,235 + Pf6N2E2_2192 0.22 -3.9 2,598,235 + Pf6N2E2_2192 0.22 -3.6 2,598,236 - Pf6N2E2_2192 0.22 -2.4 2,598,307 + Pf6N2E2_2192 0.26 -1.8 2,598,307 + Pf6N2E2_2192 0.26 -2.6 2,598,308 - Pf6N2E2_2192 0.26 -2.5 2,598,466 + Pf6N2E2_2192 0.36 -3.2 2,598,466 + Pf6N2E2_2192 0.36 -0.8 2,598,466 + Pf6N2E2_2192 0.36 -1.8 2,598,466 + Pf6N2E2_2192 0.36 -3.8 2,598,466 + Pf6N2E2_2192 0.36 -2.6 2,598,467 - Pf6N2E2_2192 0.36 -4.0 2,598,467 - Pf6N2E2_2192 0.36 -2.4 2,598,467 - Pf6N2E2_2192 0.36 -2.8 2,598,467 - Pf6N2E2_2192 0.36 -2.8 2,598,467 - Pf6N2E2_2192 0.36 -4.2 2,598,467 - Pf6N2E2_2192 0.36 -3.7 2,598,628 + Pf6N2E2_2192 0.46 -2.5 2,598,628 + Pf6N2E2_2192 0.46 -3.9 2,598,629 - Pf6N2E2_2192 0.46 -3.7 2,598,755 - Pf6N2E2_2192 0.54 -3.2 2,599,005 - Pf6N2E2_2192 0.70 -2.5 2,599,039 + Pf6N2E2_2192 0.72 -3.4 2,599,039 + Pf6N2E2_2192 0.72 -0.8 2,599,040 - Pf6N2E2_2192 0.72 -0.2 2,599,150 + Pf6N2E2_2192 0.79 -2.4 2,599,150 + Pf6N2E2_2192 0.79 -1.2 2,599,151 - Pf6N2E2_2192 0.79 -3.6 2,599,339 + -1.9 2,599,340 - -3.4 2,599,340 - -0.8 2,599,360 + -0.2 2,599,361 - -3.1 2,599,361 - -2.6 2,599,361 - -3.5 2,599,363 + -3.5 2,599,363 + -3.8 2,599,363 + -2.9 2,599,363 + -3.0 2,599,364 - -1.5 2,599,364 - -1.8 2,599,493 - -4.7 2,599,513 + -2.9 2,599,745 + Pf6N2E2_2193 0.29 -1.6 2,599,790 + Pf6N2E2_2193 0.34 -4.0 2,599,790 + Pf6N2E2_2193 0.34 -2.2 2,599,790 + Pf6N2E2_2193 0.34 -3.1 2,599,790 + Pf6N2E2_2193 0.34 -0.5 2,599,790 + Pf6N2E2_2193 0.34 -2.9 2,599,791 - Pf6N2E2_2193 0.35 -3.6 2,599,791 - Pf6N2E2_2193 0.35 -2.9 2,599,791 - Pf6N2E2_2193 0.35 -4.2 2,599,791 - Pf6N2E2_2193 0.35 -4.3 2,599,791 - Pf6N2E2_2193 0.35 -2.3 2,599,864 + Pf6N2E2_2193 0.43 -2.6 2,599,997 + Pf6N2E2_2193 0.60 -3.1 2,599,998 - Pf6N2E2_2193 0.60 -3.4 2,600,201 + Pf6N2E2_2193 0.85 -1.6
Or see this region's nucleotide sequence