Experiment: L-Leucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2190 and Pf6N2E2_2191 are separated by 198 nucleotides Pf6N2E2_2191 and Pf6N2E2_2192 are separated by 11 nucleotides
Pf6N2E2_2190 - Acetoacetyl-CoA synthetase (EC 6.2.1.16) / Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3), at 2,594,816 to 2,596,513
_2190
Pf6N2E2_2191 - Isovaleryl-CoA dehydrogenase (EC 1.3.8.4) (from data) , at 2,596,712 to 2,597,875
_2191
Pf6N2E2_2192 - 3-methylcrotonyl-CoA carboxylase, beta subunit (EC 6.4.1.4) (from data) , at 2,597,887 to 2,599,494
_2192
Position (kb)
2596
2597
2598 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 2595.741 kb on + strand, within Pf6N2E2_2190 at 2595.741 kb on + strand, within Pf6N2E2_2190 at 2595.804 kb on + strand, within Pf6N2E2_2190 at 2595.804 kb on + strand, within Pf6N2E2_2190 at 2595.805 kb on - strand, within Pf6N2E2_2190 at 2595.821 kb on - strand, within Pf6N2E2_2190 at 2595.873 kb on + strand, within Pf6N2E2_2190 at 2595.873 kb on + strand, within Pf6N2E2_2190 at 2595.874 kb on - strand, within Pf6N2E2_2190 at 2595.874 kb on - strand, within Pf6N2E2_2190 at 2595.874 kb on - strand, within Pf6N2E2_2190 at 2596.164 kb on + strand, within Pf6N2E2_2190 at 2596.165 kb on - strand, within Pf6N2E2_2190 at 2596.165 kb on - strand, within Pf6N2E2_2190 at 2596.165 kb on - strand, within Pf6N2E2_2190 at 2596.185 kb on + strand, within Pf6N2E2_2190 at 2596.302 kb on - strand, within Pf6N2E2_2190 at 2596.302 kb on - strand, within Pf6N2E2_2190 at 2596.357 kb on + strand at 2596.358 kb on - strand at 2596.358 kb on - strand at 2596.358 kb on - strand at 2596.358 kb on - strand at 2596.358 kb on - strand at 2596.358 kb on - strand at 2596.359 kb on + strand at 2596.479 kb on + strand at 2596.479 kb on + strand at 2596.480 kb on - strand at 2596.480 kb on - strand at 2596.532 kb on + strand at 2596.533 kb on - strand at 2596.533 kb on - strand at 2596.536 kb on - strand at 2596.545 kb on - strand at 2596.584 kb on + strand at 2596.584 kb on + strand at 2596.585 kb on - strand at 2596.585 kb on - strand at 2596.585 kb on - strand at 2596.585 kb on - strand at 2596.585 kb on - strand at 2596.611 kb on - strand at 2596.611 kb on - strand at 2596.611 kb on - strand at 2596.611 kb on - strand at 2596.643 kb on + strand at 2596.653 kb on + strand at 2596.654 kb on - strand at 2596.901 kb on + strand, within Pf6N2E2_2191 at 2596.922 kb on + strand, within Pf6N2E2_2191 at 2596.923 kb on - strand, within Pf6N2E2_2191 at 2596.986 kb on - strand, within Pf6N2E2_2191 at 2596.986 kb on - strand, within Pf6N2E2_2191 at 2597.054 kb on + strand, within Pf6N2E2_2191 at 2597.055 kb on - strand, within Pf6N2E2_2191 at 2597.168 kb on + strand, within Pf6N2E2_2191 at 2597.168 kb on + strand, within Pf6N2E2_2191 at 2597.168 kb on + strand, within Pf6N2E2_2191 at 2597.169 kb on - strand, within Pf6N2E2_2191 at 2597.169 kb on - strand, within Pf6N2E2_2191 at 2597.169 kb on - strand, within Pf6N2E2_2191 at 2597.169 kb on - strand, within Pf6N2E2_2191 at 2597.169 kb on - strand, within Pf6N2E2_2191 at 2597.222 kb on + strand, within Pf6N2E2_2191 at 2597.222 kb on + strand, within Pf6N2E2_2191 at 2597.223 kb on - strand, within Pf6N2E2_2191 at 2597.223 kb on - strand, within Pf6N2E2_2191 at 2597.232 kb on - strand, within Pf6N2E2_2191 at 2597.232 kb on - strand, within Pf6N2E2_2191 at 2597.317 kb on + strand, within Pf6N2E2_2191 at 2597.318 kb on - strand, within Pf6N2E2_2191 at 2597.318 kb on - strand, within Pf6N2E2_2191 at 2597.516 kb on + strand, within Pf6N2E2_2191 at 2597.517 kb on - strand, within Pf6N2E2_2191 at 2597.517 kb on - strand, within Pf6N2E2_2191 at 2597.517 kb on - strand, within Pf6N2E2_2191 at 2597.591 kb on + strand, within Pf6N2E2_2191 at 2597.591 kb on + strand, within Pf6N2E2_2191 at 2597.591 kb on + strand, within Pf6N2E2_2191 at 2597.591 kb on + strand, within Pf6N2E2_2191 at 2597.591 kb on + strand, within Pf6N2E2_2191 at 2597.591 kb on + strand, within Pf6N2E2_2191 at 2597.591 kb on + strand, within Pf6N2E2_2191 at 2597.591 kb on + strand, within Pf6N2E2_2191 at 2597.591 kb on + strand, within Pf6N2E2_2191 at 2597.591 kb on + strand, within Pf6N2E2_2191 at 2597.591 kb on + strand, within Pf6N2E2_2191 at 2597.591 kb on + strand, within Pf6N2E2_2191 at 2597.591 kb on + strand, within Pf6N2E2_2191 at 2597.592 kb on - strand, within Pf6N2E2_2191 at 2597.592 kb on - strand, within Pf6N2E2_2191 at 2597.592 kb on - strand, within Pf6N2E2_2191 at 2597.592 kb on - strand, within Pf6N2E2_2191 at 2597.592 kb on - strand, within Pf6N2E2_2191 at 2597.592 kb on - strand, within Pf6N2E2_2191 at 2597.592 kb on - strand, within Pf6N2E2_2191 at 2597.624 kb on + strand, within Pf6N2E2_2191 at 2597.624 kb on + strand, within Pf6N2E2_2191 at 2597.624 kb on + strand, within Pf6N2E2_2191 at 2597.624 kb on + strand, within Pf6N2E2_2191 at 2597.625 kb on - strand, within Pf6N2E2_2191 at 2597.625 kb on - strand, within Pf6N2E2_2191 at 2597.690 kb on + strand, within Pf6N2E2_2191 at 2597.690 kb on + strand, within Pf6N2E2_2191 at 2597.691 kb on - strand, within Pf6N2E2_2191 at 2597.753 kb on + strand, within Pf6N2E2_2191 at 2597.753 kb on + strand, within Pf6N2E2_2191 at 2597.753 kb on + strand, within Pf6N2E2_2191 at 2597.753 kb on + strand, within Pf6N2E2_2191 at 2597.754 kb on - strand, within Pf6N2E2_2191 at 2597.754 kb on - strand, within Pf6N2E2_2191 at 2597.754 kb on - strand, within Pf6N2E2_2191 at 2597.754 kb on - strand, within Pf6N2E2_2191 at 2597.802 kb on - strand at 2598.047 kb on + strand at 2598.048 kb on - strand, within Pf6N2E2_2192 at 2598.048 kb on - strand, within Pf6N2E2_2192 at 2598.235 kb on + strand, within Pf6N2E2_2192 at 2598.235 kb on + strand, within Pf6N2E2_2192 at 2598.235 kb on + strand, within Pf6N2E2_2192 at 2598.235 kb on + strand, within Pf6N2E2_2192 at 2598.235 kb on + strand, within Pf6N2E2_2192 at 2598.236 kb on - strand, within Pf6N2E2_2192 at 2598.307 kb on + strand, within Pf6N2E2_2192 at 2598.307 kb on + strand, within Pf6N2E2_2192 at 2598.308 kb on - strand, within Pf6N2E2_2192 at 2598.466 kb on + strand, within Pf6N2E2_2192 at 2598.466 kb on + strand, within Pf6N2E2_2192 at 2598.466 kb on + strand, within Pf6N2E2_2192 at 2598.466 kb on + strand, within Pf6N2E2_2192 at 2598.466 kb on + strand, within Pf6N2E2_2192 at 2598.467 kb on - strand, within Pf6N2E2_2192 at 2598.467 kb on - strand, within Pf6N2E2_2192 at 2598.467 kb on - strand, within Pf6N2E2_2192 at 2598.467 kb on - strand, within Pf6N2E2_2192 at 2598.467 kb on - strand, within Pf6N2E2_2192 at 2598.467 kb on - strand, within Pf6N2E2_2192 at 2598.628 kb on + strand, within Pf6N2E2_2192 at 2598.628 kb on + strand, within Pf6N2E2_2192 at 2598.629 kb on - strand, within Pf6N2E2_2192 at 2598.755 kb on - strand, within Pf6N2E2_2192
Per-strain Table
Position Strand Gene LocusTag Fraction L-Leucine (C) remove 2,595,741 + Pf6N2E2_2190 0.54 -0.6 2,595,741 + Pf6N2E2_2190 0.54 +0.3 2,595,804 + Pf6N2E2_2190 0.58 +0.8 2,595,804 + Pf6N2E2_2190 0.58 +1.1 2,595,805 - Pf6N2E2_2190 0.58 +0.5 2,595,821 - Pf6N2E2_2190 0.59 +1.5 2,595,873 + Pf6N2E2_2190 0.62 +0.7 2,595,873 + Pf6N2E2_2190 0.62 +1.0 2,595,874 - Pf6N2E2_2190 0.62 +1.2 2,595,874 - Pf6N2E2_2190 0.62 +1.4 2,595,874 - Pf6N2E2_2190 0.62 -0.4 2,596,164 + Pf6N2E2_2190 0.79 +1.8 2,596,165 - Pf6N2E2_2190 0.79 +2.0 2,596,165 - Pf6N2E2_2190 0.79 +0.6 2,596,165 - Pf6N2E2_2190 0.79 +0.3 2,596,185 + Pf6N2E2_2190 0.81 +0.9 2,596,302 - Pf6N2E2_2190 0.88 -0.8 2,596,302 - Pf6N2E2_2190 0.88 -0.7 2,596,357 + +0.2 2,596,358 - -0.5 2,596,358 - +0.8 2,596,358 - +1.2 2,596,358 - +0.8 2,596,358 - -0.2 2,596,358 - -0.5 2,596,359 + +0.7 2,596,479 + +0.1 2,596,479 + +1.8 2,596,480 - +1.2 2,596,480 - -0.6 2,596,532 + +0.3 2,596,533 - -1.0 2,596,533 - +0.5 2,596,536 - +0.8 2,596,545 - -1.2 2,596,584 + -0.2 2,596,584 + -0.1 2,596,585 - -0.5 2,596,585 - -1.2 2,596,585 - -1.9 2,596,585 - -0.1 2,596,585 - -1.4 2,596,611 - -0.8 2,596,611 - +0.4 2,596,611 - +0.8 2,596,611 - -0.2 2,596,643 + -4.2 2,596,653 + -0.5 2,596,654 - -3.4 2,596,901 + Pf6N2E2_2191 0.16 -1.5 2,596,922 + Pf6N2E2_2191 0.18 -3.1 2,596,923 - Pf6N2E2_2191 0.18 -3.3 2,596,986 - Pf6N2E2_2191 0.24 -1.5 2,596,986 - Pf6N2E2_2191 0.24 -3.7 2,597,054 + Pf6N2E2_2191 0.29 -1.9 2,597,055 - Pf6N2E2_2191 0.29 -2.5 2,597,168 + Pf6N2E2_2191 0.39 -2.9 2,597,168 + Pf6N2E2_2191 0.39 -1.2 2,597,168 + Pf6N2E2_2191 0.39 -1.8 2,597,169 - Pf6N2E2_2191 0.39 -2.8 2,597,169 - Pf6N2E2_2191 0.39 -2.6 2,597,169 - Pf6N2E2_2191 0.39 -4.2 2,597,169 - Pf6N2E2_2191 0.39 -5.0 2,597,169 - Pf6N2E2_2191 0.39 -1.8 2,597,222 + Pf6N2E2_2191 0.44 -1.5 2,597,222 + Pf6N2E2_2191 0.44 -3.5 2,597,223 - Pf6N2E2_2191 0.44 -2.5 2,597,223 - Pf6N2E2_2191 0.44 -3.3 2,597,232 - Pf6N2E2_2191 0.45 -1.8 2,597,232 - Pf6N2E2_2191 0.45 -2.2 2,597,317 + Pf6N2E2_2191 0.52 -4.0 2,597,318 - Pf6N2E2_2191 0.52 -3.2 2,597,318 - Pf6N2E2_2191 0.52 -2.8 2,597,516 + Pf6N2E2_2191 0.69 -1.8 2,597,517 - Pf6N2E2_2191 0.69 -2.6 2,597,517 - Pf6N2E2_2191 0.69 -3.1 2,597,517 - Pf6N2E2_2191 0.69 -3.8 2,597,591 + Pf6N2E2_2191 0.76 -2.8 2,597,591 + Pf6N2E2_2191 0.76 +0.4 2,597,591 + Pf6N2E2_2191 0.76 -3.5 2,597,591 + Pf6N2E2_2191 0.76 -4.3 2,597,591 + Pf6N2E2_2191 0.76 -1.5 2,597,591 + Pf6N2E2_2191 0.76 -4.4 2,597,591 + Pf6N2E2_2191 0.76 -1.2 2,597,591 + Pf6N2E2_2191 0.76 -3.0 2,597,591 + Pf6N2E2_2191 0.76 -2.5 2,597,591 + Pf6N2E2_2191 0.76 -2.8 2,597,591 + Pf6N2E2_2191 0.76 -0.8 2,597,591 + Pf6N2E2_2191 0.76 -1.5 2,597,591 + Pf6N2E2_2191 0.76 -4.0 2,597,592 - Pf6N2E2_2191 0.76 -3.6 2,597,592 - Pf6N2E2_2191 0.76 -3.4 2,597,592 - Pf6N2E2_2191 0.76 -1.7 2,597,592 - Pf6N2E2_2191 0.76 -3.8 2,597,592 - Pf6N2E2_2191 0.76 -3.2 2,597,592 - Pf6N2E2_2191 0.76 -2.0 2,597,592 - Pf6N2E2_2191 0.76 -2.2 2,597,624 + Pf6N2E2_2191 0.78 -2.5 2,597,624 + Pf6N2E2_2191 0.78 -2.4 2,597,624 + Pf6N2E2_2191 0.78 -3.1 2,597,624 + Pf6N2E2_2191 0.78 -4.5 2,597,625 - Pf6N2E2_2191 0.78 -2.3 2,597,625 - Pf6N2E2_2191 0.78 -2.0 2,597,690 + Pf6N2E2_2191 0.84 -2.0 2,597,690 + Pf6N2E2_2191 0.84 -4.0 2,597,691 - Pf6N2E2_2191 0.84 -3.7 2,597,753 + Pf6N2E2_2191 0.89 -3.0 2,597,753 + Pf6N2E2_2191 0.89 -0.5 2,597,753 + Pf6N2E2_2191 0.89 -5.2 2,597,753 + Pf6N2E2_2191 0.89 -3.0 2,597,754 - Pf6N2E2_2191 0.90 -2.1 2,597,754 - Pf6N2E2_2191 0.90 -4.2 2,597,754 - Pf6N2E2_2191 0.90 -2.4 2,597,754 - Pf6N2E2_2191 0.90 -4.2 2,597,802 - -3.6 2,598,047 + -2.8 2,598,048 - Pf6N2E2_2192 0.10 -2.9 2,598,048 - Pf6N2E2_2192 0.10 -3.1 2,598,235 + Pf6N2E2_2192 0.22 -3.1 2,598,235 + Pf6N2E2_2192 0.22 -3.8 2,598,235 + Pf6N2E2_2192 0.22 -2.8 2,598,235 + Pf6N2E2_2192 0.22 -3.9 2,598,235 + Pf6N2E2_2192 0.22 -3.6 2,598,236 - Pf6N2E2_2192 0.22 -2.4 2,598,307 + Pf6N2E2_2192 0.26 -1.8 2,598,307 + Pf6N2E2_2192 0.26 -2.6 2,598,308 - Pf6N2E2_2192 0.26 -2.5 2,598,466 + Pf6N2E2_2192 0.36 -3.2 2,598,466 + Pf6N2E2_2192 0.36 -0.8 2,598,466 + Pf6N2E2_2192 0.36 -1.8 2,598,466 + Pf6N2E2_2192 0.36 -3.8 2,598,466 + Pf6N2E2_2192 0.36 -2.6 2,598,467 - Pf6N2E2_2192 0.36 -4.0 2,598,467 - Pf6N2E2_2192 0.36 -2.4 2,598,467 - Pf6N2E2_2192 0.36 -2.8 2,598,467 - Pf6N2E2_2192 0.36 -2.8 2,598,467 - Pf6N2E2_2192 0.36 -4.2 2,598,467 - Pf6N2E2_2192 0.36 -3.7 2,598,628 + Pf6N2E2_2192 0.46 -2.5 2,598,628 + Pf6N2E2_2192 0.46 -3.9 2,598,629 - Pf6N2E2_2192 0.46 -3.7 2,598,755 - Pf6N2E2_2192 0.54 -3.2
Or see this region's nucleotide sequence