Experiment: L-Leucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1831 and Pf6N2E2_1832 overlap by 4 nucleotides Pf6N2E2_1832 and Pf6N2E2_1833 overlap by 8 nucleotides Pf6N2E2_1833 and Pf6N2E2_1834 are separated by 2 nucleotides Pf6N2E2_1834 and Pf6N2E2_1835 overlap by 4 nucleotides
Pf6N2E2_1831 - Citrate lyase beta chain (EC 4.1.3.6), at 2,169,405 to 2,170,304
_1831
Pf6N2E2_1832 - COGs COG3777, at 2,170,301 to 2,171,158
_1832
Pf6N2E2_1833 - 3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35) / Enoyl CoA hydratase (EC 4.2.1.17), at 2,171,151 to 2,172,668
_1833
Pf6N2E2_1834 - Enoyl-CoA hydratase (EC 4.2.1.17), at 2,172,671 to 2,173,441
_1834
Pf6N2E2_1835 - Enoyl-CoA hydratase (EC 4.2.1.17), at 2,173,438 to 2,174,049
_1835
Position (kb)
2171
2172
2173 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2170.253 kb on + strand at 2170.406 kb on + strand, within Pf6N2E2_1832 at 2170.407 kb on - strand, within Pf6N2E2_1832 at 2170.407 kb on - strand, within Pf6N2E2_1832 at 2170.407 kb on - strand, within Pf6N2E2_1832 at 2170.463 kb on + strand, within Pf6N2E2_1832 at 2170.500 kb on - strand, within Pf6N2E2_1832 at 2170.572 kb on + strand, within Pf6N2E2_1832 at 2170.649 kb on - strand, within Pf6N2E2_1832 at 2170.903 kb on + strand, within Pf6N2E2_1832 at 2170.903 kb on + strand, within Pf6N2E2_1832 at 2170.903 kb on + strand, within Pf6N2E2_1832 at 2170.903 kb on + strand, within Pf6N2E2_1832 at 2170.903 kb on + strand, within Pf6N2E2_1832 at 2170.903 kb on + strand, within Pf6N2E2_1832 at 2170.903 kb on + strand, within Pf6N2E2_1832 at 2170.904 kb on - strand, within Pf6N2E2_1832 at 2170.904 kb on - strand, within Pf6N2E2_1832 at 2170.996 kb on - strand, within Pf6N2E2_1832 at 2171.036 kb on - strand, within Pf6N2E2_1832 at 2171.036 kb on - strand, within Pf6N2E2_1832 at 2171.136 kb on + strand at 2171.136 kb on + strand at 2171.136 kb on + strand at 2171.137 kb on - strand at 2171.181 kb on + strand at 2171.655 kb on + strand, within Pf6N2E2_1833 at 2171.655 kb on + strand, within Pf6N2E2_1833 at 2171.657 kb on + strand, within Pf6N2E2_1833 at 2171.926 kb on + strand, within Pf6N2E2_1833 at 2171.926 kb on + strand, within Pf6N2E2_1833 at 2172.079 kb on + strand, within Pf6N2E2_1833 at 2172.081 kb on + strand, within Pf6N2E2_1833 at 2172.156 kb on + strand, within Pf6N2E2_1833 at 2172.156 kb on + strand, within Pf6N2E2_1833 at 2172.317 kb on + strand, within Pf6N2E2_1833 at 2172.317 kb on + strand, within Pf6N2E2_1833 at 2172.318 kb on - strand, within Pf6N2E2_1833 at 2172.318 kb on - strand, within Pf6N2E2_1833 at 2172.506 kb on + strand, within Pf6N2E2_1833 at 2172.574 kb on + strand at 2172.575 kb on - strand at 2172.575 kb on - strand at 2172.663 kb on - strand at 2172.668 kb on + strand at 2172.668 kb on + strand at 2172.669 kb on - strand at 2172.669 kb on - strand at 2172.672 kb on + strand at 2172.673 kb on - strand at 2173.007 kb on + strand, within Pf6N2E2_1834 at 2173.239 kb on - strand, within Pf6N2E2_1834 at 2173.399 kb on + strand at 2173.442 kb on - strand at 2173.442 kb on - strand at 2173.448 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Leucine (C) remove 2,170,253 + -1.5 2,170,406 + Pf6N2E2_1832 0.12 -0.8 2,170,407 - Pf6N2E2_1832 0.12 -0.5 2,170,407 - Pf6N2E2_1832 0.12 +1.3 2,170,407 - Pf6N2E2_1832 0.12 +0.8 2,170,463 + Pf6N2E2_1832 0.19 -0.7 2,170,500 - Pf6N2E2_1832 0.23 -1.7 2,170,572 + Pf6N2E2_1832 0.32 +0.2 2,170,649 - Pf6N2E2_1832 0.41 +0.1 2,170,903 + Pf6N2E2_1832 0.70 +0.4 2,170,903 + Pf6N2E2_1832 0.70 +0.2 2,170,903 + Pf6N2E2_1832 0.70 +0.0 2,170,903 + Pf6N2E2_1832 0.70 -2.0 2,170,903 + Pf6N2E2_1832 0.70 +0.5 2,170,903 + Pf6N2E2_1832 0.70 +0.8 2,170,903 + Pf6N2E2_1832 0.70 +0.2 2,170,904 - Pf6N2E2_1832 0.70 -0.2 2,170,904 - Pf6N2E2_1832 0.70 -0.2 2,170,996 - Pf6N2E2_1832 0.81 -0.1 2,171,036 - Pf6N2E2_1832 0.86 -0.2 2,171,036 - Pf6N2E2_1832 0.86 -1.2 2,171,136 + -0.7 2,171,136 + -0.6 2,171,136 + -0.4 2,171,137 - +0.7 2,171,181 + -0.8 2,171,655 + Pf6N2E2_1833 0.33 -0.8 2,171,655 + Pf6N2E2_1833 0.33 -0.6 2,171,657 + Pf6N2E2_1833 0.33 -0.2 2,171,926 + Pf6N2E2_1833 0.51 -0.2 2,171,926 + Pf6N2E2_1833 0.51 -0.9 2,172,079 + Pf6N2E2_1833 0.61 -0.5 2,172,081 + Pf6N2E2_1833 0.61 -0.4 2,172,156 + Pf6N2E2_1833 0.66 -1.0 2,172,156 + Pf6N2E2_1833 0.66 -0.6 2,172,317 + Pf6N2E2_1833 0.77 -0.2 2,172,317 + Pf6N2E2_1833 0.77 -2.6 2,172,318 - Pf6N2E2_1833 0.77 +0.3 2,172,318 - Pf6N2E2_1833 0.77 +2.3 2,172,506 + Pf6N2E2_1833 0.89 -1.6 2,172,574 + +0.7 2,172,575 - -0.9 2,172,575 - +0.0 2,172,663 - -1.0 2,172,668 + -0.7 2,172,668 + -0.8 2,172,669 - -0.3 2,172,669 - -0.0 2,172,672 + -0.9 2,172,673 - -0.3 2,173,007 + Pf6N2E2_1834 0.44 +0.8 2,173,239 - Pf6N2E2_1834 0.74 +0.4 2,173,399 + -0.7 2,173,442 - +0.3 2,173,442 - +0.9 2,173,448 + +0.9
Or see this region's nucleotide sequence