Experiment: L-Leucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1750 and Pf6N2E2_1751 are separated by 64 nucleotides Pf6N2E2_1751 and Pf6N2E2_1752 are separated by 62 nucleotides
Pf6N2E2_1750 - Sigma-54 dependent transcriptional regulator, at 2,063,866 to 2,065,788
_1750
Pf6N2E2_1751 - Aldehyde dehydrogenase A (EC 1.2.1.22) / Glycolaldehyde dehydrogenase (EC 1.2.1.21), at 2,065,853 to 2,067,286
_1751
Pf6N2E2_1752 - Quino(hemo)protein alcohol dehydrogenase, PQQ-dependent (EC 1.1.99.8), at 2,067,349 to 2,069,529
_1752
Position (kb)
2065
2066
2067
2068 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2065.092 kb on + strand, within Pf6N2E2_1750 at 2065.308 kb on + strand, within Pf6N2E2_1750 at 2065.308 kb on + strand, within Pf6N2E2_1750 at 2065.309 kb on - strand, within Pf6N2E2_1750 at 2065.309 kb on - strand, within Pf6N2E2_1750 at 2065.610 kb on - strand at 2065.866 kb on + strand at 2065.866 kb on + strand at 2065.866 kb on + strand at 2065.866 kb on + strand at 2065.867 kb on - strand at 2065.893 kb on + strand at 2065.985 kb on - strand at 2065.986 kb on + strand at 2065.986 kb on + strand at 2065.986 kb on + strand at 2065.986 kb on + strand at 2065.986 kb on + strand at 2065.987 kb on - strand at 2065.987 kb on - strand at 2065.987 kb on - strand at 2065.987 kb on - strand at 2065.987 kb on - strand at 2065.987 kb on - strand at 2065.987 kb on - strand at 2065.987 kb on - strand at 2066.047 kb on - strand, within Pf6N2E2_1751 at 2066.243 kb on - strand, within Pf6N2E2_1751 at 2066.782 kb on + strand, within Pf6N2E2_1751 at 2066.955 kb on + strand, within Pf6N2E2_1751 at 2066.955 kb on + strand, within Pf6N2E2_1751 at 2066.955 kb on + strand, within Pf6N2E2_1751 at 2066.956 kb on - strand, within Pf6N2E2_1751 at 2066.956 kb on - strand, within Pf6N2E2_1751 at 2067.084 kb on + strand, within Pf6N2E2_1751 at 2067.085 kb on - strand, within Pf6N2E2_1751 at 2067.261 kb on + strand at 2067.270 kb on + strand at 2067.271 kb on - strand at 2067.350 kb on + strand at 2067.350 kb on + strand at 2067.351 kb on - strand at 2067.419 kb on + strand at 2067.420 kb on - strand at 2067.420 kb on - strand at 2067.451 kb on - strand at 2067.608 kb on + strand, within Pf6N2E2_1752 at 2067.608 kb on + strand, within Pf6N2E2_1752 at 2067.720 kb on - strand, within Pf6N2E2_1752 at 2067.720 kb on - strand, within Pf6N2E2_1752 at 2067.818 kb on + strand, within Pf6N2E2_1752 at 2067.839 kb on + strand, within Pf6N2E2_1752 at 2067.839 kb on + strand, within Pf6N2E2_1752 at 2067.840 kb on - strand, within Pf6N2E2_1752 at 2067.840 kb on - strand, within Pf6N2E2_1752 at 2067.840 kb on - strand, within Pf6N2E2_1752 at 2067.991 kb on + strand, within Pf6N2E2_1752 at 2067.991 kb on + strand, within Pf6N2E2_1752 at 2067.992 kb on - strand, within Pf6N2E2_1752 at 2068.124 kb on + strand, within Pf6N2E2_1752 at 2068.125 kb on - strand, within Pf6N2E2_1752 at 2068.125 kb on - strand, within Pf6N2E2_1752 at 2068.173 kb on - strand, within Pf6N2E2_1752 at 2068.200 kb on + strand, within Pf6N2E2_1752 at 2068.202 kb on + strand, within Pf6N2E2_1752 at 2068.202 kb on + strand, within Pf6N2E2_1752 at 2068.203 kb on - strand, within Pf6N2E2_1752 at 2068.226 kb on + strand, within Pf6N2E2_1752 at 2068.226 kb on + strand, within Pf6N2E2_1752
Per-strain Table
Position Strand Gene LocusTag Fraction L-Leucine (C) remove 2,065,092 + Pf6N2E2_1750 0.64 +1.0 2,065,308 + Pf6N2E2_1750 0.75 +0.2 2,065,308 + Pf6N2E2_1750 0.75 +0.3 2,065,309 - Pf6N2E2_1750 0.75 +0.5 2,065,309 - Pf6N2E2_1750 0.75 +0.7 2,065,610 - +0.7 2,065,866 + +1.7 2,065,866 + -2.2 2,065,866 + +0.6 2,065,866 + +0.0 2,065,867 - -1.5 2,065,893 + +0.7 2,065,985 - -0.3 2,065,986 + +0.3 2,065,986 + -1.0 2,065,986 + +1.6 2,065,986 + +0.3 2,065,986 + +0.8 2,065,987 - +0.2 2,065,987 - -0.4 2,065,987 - -0.4 2,065,987 - -0.2 2,065,987 - +0.5 2,065,987 - +0.4 2,065,987 - -0.1 2,065,987 - +0.7 2,066,047 - Pf6N2E2_1751 0.14 -0.1 2,066,243 - Pf6N2E2_1751 0.27 -0.4 2,066,782 + Pf6N2E2_1751 0.65 +0.1 2,066,955 + Pf6N2E2_1751 0.77 +0.8 2,066,955 + Pf6N2E2_1751 0.77 +0.1 2,066,955 + Pf6N2E2_1751 0.77 +1.0 2,066,956 - Pf6N2E2_1751 0.77 -1.0 2,066,956 - Pf6N2E2_1751 0.77 +0.1 2,067,084 + Pf6N2E2_1751 0.86 -2.1 2,067,085 - Pf6N2E2_1751 0.86 +0.5 2,067,261 + +1.2 2,067,270 + -1.3 2,067,271 - +2.0 2,067,350 + +0.3 2,067,350 + -0.1 2,067,351 - +0.6 2,067,419 + +0.3 2,067,420 - +0.7 2,067,420 - -1.7 2,067,451 - -0.1 2,067,608 + Pf6N2E2_1752 0.12 +0.7 2,067,608 + Pf6N2E2_1752 0.12 -0.1 2,067,720 - Pf6N2E2_1752 0.17 +1.0 2,067,720 - Pf6N2E2_1752 0.17 -0.0 2,067,818 + Pf6N2E2_1752 0.22 +1.4 2,067,839 + Pf6N2E2_1752 0.22 -0.1 2,067,839 + Pf6N2E2_1752 0.22 -0.3 2,067,840 - Pf6N2E2_1752 0.23 -0.9 2,067,840 - Pf6N2E2_1752 0.23 +2.6 2,067,840 - Pf6N2E2_1752 0.23 +1.0 2,067,991 + Pf6N2E2_1752 0.29 +0.9 2,067,991 + Pf6N2E2_1752 0.29 -0.7 2,067,992 - Pf6N2E2_1752 0.29 +0.7 2,068,124 + Pf6N2E2_1752 0.36 -0.1 2,068,125 - Pf6N2E2_1752 0.36 +0.7 2,068,125 - Pf6N2E2_1752 0.36 -0.0 2,068,173 - Pf6N2E2_1752 0.38 +1.3 2,068,200 + Pf6N2E2_1752 0.39 +0.6 2,068,202 + Pf6N2E2_1752 0.39 -0.0 2,068,202 + Pf6N2E2_1752 0.39 -0.1 2,068,203 - Pf6N2E2_1752 0.39 -0.0 2,068,226 + Pf6N2E2_1752 0.40 +0.6 2,068,226 + Pf6N2E2_1752 0.40 -0.3
Or see this region's nucleotide sequence