Experiment: L-Leucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1143 and Pf6N2E2_1144 are separated by 16 nucleotides Pf6N2E2_1144 and Pf6N2E2_1145 are separated by 13 nucleotides Pf6N2E2_1145 and Pf6N2E2_1146 are separated by 9 nucleotides
Pf6N2E2_1143 - Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases, at 1,339,620 to 1,341,293
_1143
Pf6N2E2_1144 - Probable acyl-CoA dehydrogenase (EC 1.3.99.3), at 1,341,310 to 1,342,071
_1144
Pf6N2E2_1145 - 3-ketoacyl-CoA thiolase (EC 2.3.1.16), at 1,342,085 to 1,343,275
_1145
Pf6N2E2_1146 - 2-methylbutanoyl-CoA dehydrogenase (EC 1.3.8.5) (from data) , at 1,343,285 to 1,344,412
_1146
Position (kb)
1342
1343
1344 Strain fitness (log2 ratio)
-1
0
1
2 at 1341.230 kb on + strand at 1341.231 kb on - strand at 1341.999 kb on + strand at 1341.999 kb on + strand at 1341.999 kb on + strand at 1342.000 kb on - strand at 1342.000 kb on - strand at 1342.057 kb on - strand at 1342.057 kb on - strand at 1342.069 kb on + strand at 1342.069 kb on + strand at 1342.069 kb on + strand at 1342.069 kb on + strand at 1342.069 kb on + strand at 1342.069 kb on + strand at 1342.069 kb on + strand at 1342.070 kb on - strand at 1342.070 kb on - strand at 1342.070 kb on - strand at 1342.073 kb on + strand at 1342.074 kb on - strand at 1342.074 kb on - strand at 1342.074 kb on - strand at 1342.074 kb on - strand at 1342.102 kb on + strand at 1342.102 kb on + strand at 1342.102 kb on + strand at 1342.103 kb on - strand at 1343.270 kb on + strand at 1343.270 kb on + strand at 1343.271 kb on - strand at 1343.271 kb on - strand at 1343.271 kb on - strand at 1343.273 kb on + strand at 1343.281 kb on + strand at 1343.281 kb on + strand at 1343.282 kb on - strand at 1343.282 kb on - strand at 1343.282 kb on - strand at 1343.483 kb on + strand, within Pf6N2E2_1146 at 1343.483 kb on + strand, within Pf6N2E2_1146 at 1343.720 kb on + strand, within Pf6N2E2_1146 at 1343.720 kb on + strand, within Pf6N2E2_1146 at 1343.720 kb on + strand, within Pf6N2E2_1146 at 1343.720 kb on + strand, within Pf6N2E2_1146 at 1343.720 kb on + strand, within Pf6N2E2_1146 at 1343.721 kb on - strand, within Pf6N2E2_1146 at 1343.721 kb on - strand, within Pf6N2E2_1146 at 1343.972 kb on + strand, within Pf6N2E2_1146 at 1343.972 kb on + strand, within Pf6N2E2_1146 at 1343.973 kb on - strand, within Pf6N2E2_1146 at 1344.068 kb on + strand, within Pf6N2E2_1146 at 1344.069 kb on - strand, within Pf6N2E2_1146 at 1344.179 kb on + strand, within Pf6N2E2_1146
Per-strain Table
Position Strand Gene LocusTag Fraction L-Leucine (C) remove 1,341,230 + +1.1 1,341,231 - +0.7 1,341,999 + -1.2 1,341,999 + -0.2 1,341,999 + +2.0 1,342,000 - -0.3 1,342,000 - +0.2 1,342,057 - +0.6 1,342,057 - +1.0 1,342,069 + +0.5 1,342,069 + -0.2 1,342,069 + -0.3 1,342,069 + -0.1 1,342,069 + +1.8 1,342,069 + -1.2 1,342,069 + -0.2 1,342,070 - -1.6 1,342,070 - +1.4 1,342,070 - -1.0 1,342,073 + -0.6 1,342,074 - -0.1 1,342,074 - -0.4 1,342,074 - +0.0 1,342,074 - +0.1 1,342,102 + +0.7 1,342,102 + +0.1 1,342,102 + +0.1 1,342,103 - +0.3 1,343,270 + -0.2 1,343,270 + +0.1 1,343,271 - -0.6 1,343,271 - +1.0 1,343,271 - +2.4 1,343,273 + +0.1 1,343,281 + +0.2 1,343,281 + +0.3 1,343,282 - +0.3 1,343,282 - -1.1 1,343,282 - +1.3 1,343,483 + Pf6N2E2_1146 0.18 -0.4 1,343,483 + Pf6N2E2_1146 0.18 +0.1 1,343,720 + Pf6N2E2_1146 0.39 -0.5 1,343,720 + Pf6N2E2_1146 0.39 +1.1 1,343,720 + Pf6N2E2_1146 0.39 -0.4 1,343,720 + Pf6N2E2_1146 0.39 +0.5 1,343,720 + Pf6N2E2_1146 0.39 -0.4 1,343,721 - Pf6N2E2_1146 0.39 -1.6 1,343,721 - Pf6N2E2_1146 0.39 -0.3 1,343,972 + Pf6N2E2_1146 0.61 -0.9 1,343,972 + Pf6N2E2_1146 0.61 -0.6 1,343,973 - Pf6N2E2_1146 0.61 +0.2 1,344,068 + Pf6N2E2_1146 0.69 +0.7 1,344,069 - Pf6N2E2_1146 0.70 +0.3 1,344,179 + Pf6N2E2_1146 0.79 -1.6
Or see this region's nucleotide sequence