Experiment: L-Leucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1100 and Pf6N2E2_1101 are separated by 54 nucleotides Pf6N2E2_1101 and Pf6N2E2_1102 are separated by 186 nucleotides Pf6N2E2_1102 and Pf6N2E2_1103 are separated by 11 nucleotides Pf6N2E2_1103 and Pf6N2E2_1104 overlap by 4 nucleotides
Pf6N2E2_1100 - L-rhamnose operon transcriptional activator RhaR, at 1,286,629 to 1,287,465
_1100
Pf6N2E2_1101 - Transporter, LysE family, at 1,287,520 to 1,288,122
_1101
Pf6N2E2_1102 - Aldehyde dehydrogenase A (EC 1.2.1.22), at 1,288,309 to 1,289,742
_1102
Pf6N2E2_1103 - 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase (EC 4.1.2.n4), at 1,289,754 to 1,290,554
_1103
Pf6N2E2_1104 - Gluconate dehydratase (EC 4.2.1.39), at 1,290,551 to 1,291,750
_1104
Position (kb)
1288
1289
1290 Strain fitness (log2 ratio)
-1
0
1
2
3
4 at 1287.434 kb on - strand at 1287.531 kb on - strand at 1287.603 kb on + strand, within Pf6N2E2_1101 at 1287.604 kb on - strand, within Pf6N2E2_1101 at 1287.604 kb on - strand, within Pf6N2E2_1101 at 1287.604 kb on - strand, within Pf6N2E2_1101 at 1287.604 kb on - strand, within Pf6N2E2_1101 at 1287.604 kb on - strand, within Pf6N2E2_1101 at 1287.604 kb on - strand, within Pf6N2E2_1101 at 1287.752 kb on + strand, within Pf6N2E2_1101 at 1288.009 kb on + strand, within Pf6N2E2_1101 at 1288.010 kb on - strand, within Pf6N2E2_1101 at 1288.010 kb on - strand, within Pf6N2E2_1101 at 1288.010 kb on - strand, within Pf6N2E2_1101 at 1288.300 kb on + strand at 1288.322 kb on + strand at 1288.336 kb on - strand at 1288.344 kb on - strand at 1288.349 kb on + strand at 1288.349 kb on + strand at 1288.349 kb on + strand at 1288.349 kb on + strand at 1288.350 kb on - strand at 1288.350 kb on - strand at 1288.393 kb on + strand at 1288.442 kb on + strand at 1288.442 kb on + strand at 1288.442 kb on + strand at 1288.443 kb on - strand at 1288.443 kb on - strand at 1288.443 kb on - strand at 1288.524 kb on - strand, within Pf6N2E2_1102 at 1288.629 kb on + strand, within Pf6N2E2_1102 at 1288.877 kb on + strand, within Pf6N2E2_1102 at 1288.877 kb on + strand, within Pf6N2E2_1102 at 1288.877 kb on + strand, within Pf6N2E2_1102 at 1289.408 kb on + strand, within Pf6N2E2_1102 at 1289.408 kb on + strand, within Pf6N2E2_1102 at 1289.408 kb on + strand, within Pf6N2E2_1102 at 1289.409 kb on - strand, within Pf6N2E2_1102 at 1289.409 kb on - strand, within Pf6N2E2_1102 at 1289.417 kb on + strand, within Pf6N2E2_1102 at 1289.417 kb on + strand, within Pf6N2E2_1102 at 1289.417 kb on + strand, within Pf6N2E2_1102 at 1289.417 kb on + strand, within Pf6N2E2_1102 at 1289.417 kb on + strand, within Pf6N2E2_1102 at 1289.418 kb on - strand, within Pf6N2E2_1102 at 1289.418 kb on - strand, within Pf6N2E2_1102 at 1289.669 kb on + strand at 1289.669 kb on + strand at 1289.669 kb on + strand at 1289.669 kb on + strand at 1289.669 kb on + strand at 1289.670 kb on - strand at 1289.758 kb on + strand at 1289.759 kb on - strand at 1289.973 kb on + strand, within Pf6N2E2_1103 at 1289.974 kb on - strand, within Pf6N2E2_1103 at 1289.974 kb on - strand, within Pf6N2E2_1103 at 1290.145 kb on + strand, within Pf6N2E2_1103 at 1290.146 kb on - strand, within Pf6N2E2_1103 at 1290.146 kb on - strand, within Pf6N2E2_1103 at 1290.146 kb on - strand, within Pf6N2E2_1103 at 1290.359 kb on - strand, within Pf6N2E2_1103
Per-strain Table
Position Strand Gene LocusTag Fraction L-Leucine (C) remove 1,287,434 - +0.8 1,287,531 - +3.1 1,287,603 + Pf6N2E2_1101 0.14 +3.4 1,287,604 - Pf6N2E2_1101 0.14 +2.7 1,287,604 - Pf6N2E2_1101 0.14 +3.8 1,287,604 - Pf6N2E2_1101 0.14 +3.3 1,287,604 - Pf6N2E2_1101 0.14 +2.9 1,287,604 - Pf6N2E2_1101 0.14 +2.9 1,287,604 - Pf6N2E2_1101 0.14 +1.8 1,287,752 + Pf6N2E2_1101 0.38 +1.8 1,288,009 + Pf6N2E2_1101 0.81 +3.8 1,288,010 - Pf6N2E2_1101 0.81 +2.8 1,288,010 - Pf6N2E2_1101 0.81 +2.4 1,288,010 - Pf6N2E2_1101 0.81 +1.7 1,288,300 + -0.7 1,288,322 + +0.1 1,288,336 - +0.8 1,288,344 - -0.9 1,288,349 + +2.8 1,288,349 + +0.6 1,288,349 + -0.3 1,288,349 + +1.8 1,288,350 - +0.5 1,288,350 - +0.3 1,288,393 + +0.4 1,288,442 + -1.4 1,288,442 + +0.4 1,288,442 + +1.0 1,288,443 - -0.4 1,288,443 - -1.0 1,288,443 - +0.3 1,288,524 - Pf6N2E2_1102 0.15 +1.6 1,288,629 + Pf6N2E2_1102 0.22 -0.3 1,288,877 + Pf6N2E2_1102 0.40 -1.7 1,288,877 + Pf6N2E2_1102 0.40 +0.8 1,288,877 + Pf6N2E2_1102 0.40 +0.4 1,289,408 + Pf6N2E2_1102 0.77 -0.6 1,289,408 + Pf6N2E2_1102 0.77 +0.9 1,289,408 + Pf6N2E2_1102 0.77 +0.6 1,289,409 - Pf6N2E2_1102 0.77 +0.7 1,289,409 - Pf6N2E2_1102 0.77 +1.7 1,289,417 + Pf6N2E2_1102 0.77 +0.3 1,289,417 + Pf6N2E2_1102 0.77 -1.0 1,289,417 + Pf6N2E2_1102 0.77 -0.8 1,289,417 + Pf6N2E2_1102 0.77 +0.4 1,289,417 + Pf6N2E2_1102 0.77 +0.6 1,289,418 - Pf6N2E2_1102 0.77 +0.4 1,289,418 - Pf6N2E2_1102 0.77 +0.4 1,289,669 + +0.8 1,289,669 + +0.2 1,289,669 + +0.1 1,289,669 + +1.0 1,289,669 + -0.1 1,289,670 - -1.0 1,289,758 + -0.4 1,289,759 - -0.1 1,289,973 + Pf6N2E2_1103 0.27 -0.8 1,289,974 - Pf6N2E2_1103 0.27 +1.3 1,289,974 - Pf6N2E2_1103 0.27 +1.0 1,290,145 + Pf6N2E2_1103 0.49 +0.7 1,290,146 - Pf6N2E2_1103 0.49 +1.4 1,290,146 - Pf6N2E2_1103 0.49 +1.1 1,290,146 - Pf6N2E2_1103 0.49 -1.7 1,290,359 - Pf6N2E2_1103 0.76 +1.3
Or see this region's nucleotide sequence