Experiment: L-Leucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1040 and Pf6N2E2_1041 overlap by 4 nucleotides Pf6N2E2_1041 and Pf6N2E2_1042 are separated by 87 nucleotides
Pf6N2E2_1040 - Hydroxymethylglutaryl-CoA lyase (EC 4.1.3.4), at 1,214,067 to 1,215,008
_1040
Pf6N2E2_1041 - Hydroxymethylglutaryl-CoA lyase (EC 4.1.3.4), at 1,215,005 to 1,216,204
_1041
Pf6N2E2_1042 - Nitrate/nitrite transporter, at 1,216,292 to 1,217,611
_1042
Position (kb)
1215
1216
1217 Strain fitness (log2 ratio)
-2
-1
0
1 at 1214.075 kb on + strand at 1214.076 kb on - strand at 1214.076 kb on - strand at 1214.086 kb on + strand at 1214.086 kb on + strand at 1214.086 kb on + strand at 1214.086 kb on + strand at 1214.086 kb on + strand at 1214.086 kb on + strand at 1214.086 kb on + strand at 1214.087 kb on - strand at 1214.087 kb on - strand at 1214.087 kb on - strand at 1214.090 kb on + strand at 1214.090 kb on + strand at 1214.091 kb on - strand at 1214.091 kb on - strand at 1214.091 kb on - strand at 1214.366 kb on + strand, within Pf6N2E2_1040 at 1214.513 kb on - strand, within Pf6N2E2_1040 at 1214.787 kb on - strand, within Pf6N2E2_1040 at 1215.069 kb on + strand at 1215.160 kb on + strand, within Pf6N2E2_1041 at 1215.203 kb on + strand, within Pf6N2E2_1041 at 1215.203 kb on + strand, within Pf6N2E2_1041 at 1215.203 kb on + strand, within Pf6N2E2_1041 at 1215.203 kb on + strand, within Pf6N2E2_1041 at 1215.203 kb on + strand, within Pf6N2E2_1041 at 1215.203 kb on + strand, within Pf6N2E2_1041 at 1215.377 kb on + strand, within Pf6N2E2_1041 at 1215.377 kb on + strand, within Pf6N2E2_1041 at 1215.377 kb on + strand, within Pf6N2E2_1041 at 1215.377 kb on + strand, within Pf6N2E2_1041 at 1215.377 kb on + strand, within Pf6N2E2_1041 at 1215.377 kb on + strand, within Pf6N2E2_1041 at 1215.378 kb on - strand, within Pf6N2E2_1041 at 1215.378 kb on - strand, within Pf6N2E2_1041 at 1215.378 kb on - strand, within Pf6N2E2_1041 at 1215.378 kb on - strand, within Pf6N2E2_1041 at 1215.378 kb on - strand, within Pf6N2E2_1041 at 1215.410 kb on + strand, within Pf6N2E2_1041 at 1215.464 kb on + strand, within Pf6N2E2_1041 at 1215.464 kb on + strand, within Pf6N2E2_1041 at 1215.464 kb on + strand, within Pf6N2E2_1041 at 1215.465 kb on - strand, within Pf6N2E2_1041 at 1215.465 kb on - strand, within Pf6N2E2_1041 at 1215.465 kb on - strand, within Pf6N2E2_1041 at 1215.465 kb on - strand, within Pf6N2E2_1041 at 1215.713 kb on + strand, within Pf6N2E2_1041 at 1215.713 kb on + strand, within Pf6N2E2_1041 at 1215.713 kb on + strand, within Pf6N2E2_1041 at 1215.713 kb on + strand, within Pf6N2E2_1041 at 1215.728 kb on - strand at 1216.155 kb on - strand at 1216.264 kb on - strand at 1216.266 kb on - strand at 1216.272 kb on - strand at 1216.272 kb on - strand at 1216.415 kb on + strand at 1216.416 kb on - strand at 1216.416 kb on - strand at 1216.427 kb on + strand, within Pf6N2E2_1042 at 1216.428 kb on - strand, within Pf6N2E2_1042 at 1216.439 kb on - strand, within Pf6N2E2_1042 at 1216.519 kb on + strand, within Pf6N2E2_1042 at 1216.519 kb on + strand, within Pf6N2E2_1042 at 1216.519 kb on + strand, within Pf6N2E2_1042 at 1216.520 kb on - strand, within Pf6N2E2_1042 at 1216.520 kb on - strand, within Pf6N2E2_1042 at 1216.520 kb on - strand, within Pf6N2E2_1042 at 1216.520 kb on - strand, within Pf6N2E2_1042 at 1216.520 kb on - strand, within Pf6N2E2_1042 at 1216.520 kb on - strand, within Pf6N2E2_1042 at 1216.568 kb on + strand, within Pf6N2E2_1042 at 1216.655 kb on + strand, within Pf6N2E2_1042 at 1216.655 kb on + strand, within Pf6N2E2_1042 at 1216.664 kb on + strand, within Pf6N2E2_1042 at 1216.665 kb on - strand, within Pf6N2E2_1042 at 1217.108 kb on + strand, within Pf6N2E2_1042 at 1217.192 kb on + strand, within Pf6N2E2_1042
Per-strain Table
Position Strand Gene LocusTag Fraction L-Leucine (C) remove 1,214,075 + +1.3 1,214,076 - +0.5 1,214,076 - -0.2 1,214,086 + -0.3 1,214,086 + -0.8 1,214,086 + +0.9 1,214,086 + -0.1 1,214,086 + -0.4 1,214,086 + -0.4 1,214,086 + +1.0 1,214,087 - +0.8 1,214,087 - -0.7 1,214,087 - -0.2 1,214,090 + +0.0 1,214,090 + +0.5 1,214,091 - -0.9 1,214,091 - +0.1 1,214,091 - -1.0 1,214,366 + Pf6N2E2_1040 0.32 +0.7 1,214,513 - Pf6N2E2_1040 0.47 +1.2 1,214,787 - Pf6N2E2_1040 0.76 -2.3 1,215,069 + -0.7 1,215,160 + Pf6N2E2_1041 0.13 -0.4 1,215,203 + Pf6N2E2_1041 0.17 -0.3 1,215,203 + Pf6N2E2_1041 0.17 +0.8 1,215,203 + Pf6N2E2_1041 0.17 +0.7 1,215,203 + Pf6N2E2_1041 0.17 -0.3 1,215,203 + Pf6N2E2_1041 0.17 +1.2 1,215,203 + Pf6N2E2_1041 0.17 +0.2 1,215,377 + Pf6N2E2_1041 0.31 +1.1 1,215,377 + Pf6N2E2_1041 0.31 +0.3 1,215,377 + Pf6N2E2_1041 0.31 -0.4 1,215,377 + Pf6N2E2_1041 0.31 -1.1 1,215,377 + Pf6N2E2_1041 0.31 +0.1 1,215,377 + Pf6N2E2_1041 0.31 -0.6 1,215,378 - Pf6N2E2_1041 0.31 -0.0 1,215,378 - Pf6N2E2_1041 0.31 +1.5 1,215,378 - Pf6N2E2_1041 0.31 +0.9 1,215,378 - Pf6N2E2_1041 0.31 -0.7 1,215,378 - Pf6N2E2_1041 0.31 -0.1 1,215,410 + Pf6N2E2_1041 0.34 +0.7 1,215,464 + Pf6N2E2_1041 0.38 -0.1 1,215,464 + Pf6N2E2_1041 0.38 +1.0 1,215,464 + Pf6N2E2_1041 0.38 -0.4 1,215,465 - Pf6N2E2_1041 0.38 -0.5 1,215,465 - Pf6N2E2_1041 0.38 +0.3 1,215,465 - Pf6N2E2_1041 0.38 -1.4 1,215,465 - Pf6N2E2_1041 0.38 +1.1 1,215,713 + Pf6N2E2_1041 0.59 +0.9 1,215,713 + Pf6N2E2_1041 0.59 +0.4 1,215,713 + Pf6N2E2_1041 0.59 +1.5 1,215,713 + Pf6N2E2_1041 0.59 +0.9 1,215,728 - +0.5 1,216,155 - -0.5 1,216,264 - +1.3 1,216,266 - +0.9 1,216,272 - +1.4 1,216,272 - +1.0 1,216,415 + -1.1 1,216,416 - +0.4 1,216,416 - +0.3 1,216,427 + Pf6N2E2_1042 0.10 -0.6 1,216,428 - Pf6N2E2_1042 0.10 +0.0 1,216,439 - Pf6N2E2_1042 0.11 +1.1 1,216,519 + Pf6N2E2_1042 0.17 +0.5 1,216,519 + Pf6N2E2_1042 0.17 +1.3 1,216,519 + Pf6N2E2_1042 0.17 +0.1 1,216,520 - Pf6N2E2_1042 0.17 +1.2 1,216,520 - Pf6N2E2_1042 0.17 +0.7 1,216,520 - Pf6N2E2_1042 0.17 -1.8 1,216,520 - Pf6N2E2_1042 0.17 -1.7 1,216,520 - Pf6N2E2_1042 0.17 -0.8 1,216,520 - Pf6N2E2_1042 0.17 +0.5 1,216,568 + Pf6N2E2_1042 0.21 +0.3 1,216,655 + Pf6N2E2_1042 0.28 +0.3 1,216,655 + Pf6N2E2_1042 0.28 -2.3 1,216,664 + Pf6N2E2_1042 0.28 -0.5 1,216,665 - Pf6N2E2_1042 0.28 -0.1 1,217,108 + Pf6N2E2_1042 0.62 +0.9 1,217,192 + Pf6N2E2_1042 0.68 -1.0
Or see this region's nucleotide sequence