Experiment: Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma harzianum T22 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 1x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS21340 and BWI76_RS21345 overlap by 4 nucleotides BWI76_RS21345 and BWI76_RS21350 overlap by 1 nucleotides BWI76_RS21350 and BWI76_RS21355 are separated by 32 nucleotides
BWI76_RS21340: BWI76_RS21340 - SoxR reducing system protein RseC, at 4,467,978 to 4,468,457
_RS21340
BWI76_RS21345: BWI76_RS21345 - sigma-E factor regulatory protein RseB, at 4,468,454 to 4,469,410
_RS21345
BWI76_RS21350: BWI76_RS21350 - anti-RNA polymerase sigma factor SigE, at 4,469,410 to 4,470,060
_RS21350
BWI76_RS21355: BWI76_RS21355 - RNA polymerase sigma factor RpoE, at 4,470,093 to 4,470,668
_RS21355
Position (kb)
4469
4470
4471 Strain fitness (log2 ratio)
-2
-1
0
1 at 4468.534 kb on - strand at 4468.867 kb on + strand, within BWI76_RS21345 at 4468.875 kb on - strand, within BWI76_RS21345 at 4469.057 kb on - strand, within BWI76_RS21345 at 4469.491 kb on - strand, within BWI76_RS21350 at 4469.752 kb on - strand, within BWI76_RS21350 at 4469.807 kb on + strand, within BWI76_RS21350 at 4470.071 kb on - strand at 4470.738 kb on - strand at 4470.757 kb on - strand at 4471.004 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma harzianum T22 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 1x remove 4,468,534 - -0.1 4,468,867 + BWI76_RS21345 0.43 -1.7 4,468,875 - BWI76_RS21345 0.44 -1.9 4,469,057 - BWI76_RS21345 0.63 +0.5 4,469,491 - BWI76_RS21350 0.12 -0.2 4,469,752 - BWI76_RS21350 0.53 -2.0 4,469,807 + BWI76_RS21350 0.61 -1.1 4,470,071 - -0.2 4,470,738 - -1.0 4,470,757 - +0.0 4,471,004 - +0.0
Or see this region's nucleotide sequence