Experiment: Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma harzianum T22 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 1x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS12415 and BWI76_RS12420 overlap by 4 nucleotides BWI76_RS12420 and BWI76_RS12425 are separated by 363 nucleotides BWI76_RS12425 and BWI76_RS12430 are separated by 169 nucleotides
BWI76_RS12415: BWI76_RS12415 - antimicrobial peptide ABC transporter permease SapB, at 2,593,043 to 2,594,008
_RS12415
BWI76_RS12420: BWI76_RS12420 - peptide ABC transporter substrate-binding protein SapA, at 2,594,005 to 2,595,648
_RS12420
BWI76_RS12425: BWI76_RS12425 - hypothetical protein, at 2,596,012 to 2,596,296
_RS12425
BWI76_RS12430: BWI76_RS12430 - AraC family transcriptional regulator, at 2,596,466 to 2,598,772
_RS12430
Position (kb)
2594
2595
2596 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2594.126 kb on - strand at 2594.815 kb on - strand, within BWI76_RS12420 at 2595.881 kb on + strand at 2595.889 kb on - strand at 2595.889 kb on - strand at 2595.889 kb on - strand at 2596.004 kb on - strand at 2596.086 kb on - strand, within BWI76_RS12425 at 2596.105 kb on - strand, within BWI76_RS12425 at 2596.115 kb on - strand, within BWI76_RS12425 at 2596.244 kb on + strand, within BWI76_RS12425 at 2596.252 kb on - strand, within BWI76_RS12425 at 2596.594 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma harzianum T22 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 1x remove 2,594,126 - +1.1 2,594,815 - BWI76_RS12420 0.49 -1.8 2,595,881 + +0.2 2,595,889 - +0.4 2,595,889 - -0.6 2,595,889 - +0.7 2,596,004 - -0.8 2,596,086 - BWI76_RS12425 0.26 -0.2 2,596,105 - BWI76_RS12425 0.33 -0.1 2,596,115 - BWI76_RS12425 0.36 -0.0 2,596,244 + BWI76_RS12425 0.81 +0.1 2,596,252 - BWI76_RS12425 0.84 +0.3 2,596,594 + -3.2
Or see this region's nucleotide sequence