Experiment: L-Asparagine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_394 and Pf6N2E2_395 are separated by 337 nucleotides Pf6N2E2_395 and Pf6N2E2_396 are separated by 684 nucleotides
Pf6N2E2_394 - 3-oxoacyl-[acyl-carrier protein] reductase (EC 1.1.1.100), at 462,283 to 463,059
_394
Pf6N2E2_395 - Glutathione S-transferase (EC 2.5.1.18), at 463,397 to 463,828
_395
Pf6N2E2_396 - hypothetical protein, at 464,513 to 464,920
_396
Position (kb) 463
464 Strain fitness (log2 ratio) -2
-1
0
1
2 at 462.648 kb on + strand, within Pf6N2E2_394 at 462.720 kb on + strand, within Pf6N2E2_394 at 462.721 kb on - strand, within Pf6N2E2_394 at 462.721 kb on - strand, within Pf6N2E2_394 at 462.721 kb on - strand, within Pf6N2E2_394 at 462.731 kb on - strand, within Pf6N2E2_394 at 462.760 kb on + strand, within Pf6N2E2_394 at 462.761 kb on - strand, within Pf6N2E2_394 at 462.882 kb on + strand, within Pf6N2E2_394 at 462.890 kb on - strand, within Pf6N2E2_394 at 463.008 kb on + strand at 463.009 kb on - strand at 463.009 kb on - strand at 463.082 kb on + strand at 463.082 kb on + strand at 463.082 kb on + strand at 463.083 kb on - strand at 463.083 kb on - strand at 463.083 kb on - strand at 463.083 kb on - strand at 463.170 kb on - strand at 463.184 kb on + strand at 463.271 kb on + strand at 463.271 kb on + strand at 463.272 kb on - strand at 463.272 kb on - strand at 463.435 kb on - strand at 463.558 kb on + strand, within Pf6N2E2_395 at 463.602 kb on + strand, within Pf6N2E2_395 at 463.603 kb on - strand, within Pf6N2E2_395 at 463.697 kb on + strand, within Pf6N2E2_395 at 463.698 kb on - strand, within Pf6N2E2_395 at 463.731 kb on - strand, within Pf6N2E2_395 at 463.795 kb on - strand at 463.795 kb on - strand at 463.882 kb on - strand at 463.924 kb on - strand at 463.957 kb on - strand at 464.009 kb on - strand at 464.009 kb on - strand at 464.475 kb on + strand at 464.476 kb on - strand at 464.508 kb on - strand at 464.508 kb on - strand at 464.508 kb on - strand at 464.508 kb on - strand at 464.511 kb on + strand at 464.511 kb on + strand at 464.511 kb on + strand at 464.511 kb on + strand at 464.512 kb on - strand at 464.512 kb on - strand at 464.532 kb on + strand at 464.532 kb on + strand at 464.533 kb on - strand at 464.533 kb on - strand at 464.594 kb on - strand, within Pf6N2E2_396 at 464.594 kb on - strand, within Pf6N2E2_396 at 464.594 kb on - strand, within Pf6N2E2_396 at 464.690 kb on + strand, within Pf6N2E2_396 at 464.719 kb on - strand, within Pf6N2E2_396 at 464.719 kb on - strand, within Pf6N2E2_396 at 464.812 kb on + strand, within Pf6N2E2_396 at 464.812 kb on + strand, within Pf6N2E2_396 at 464.812 kb on + strand, within Pf6N2E2_396
Per-strain Table
Position Strand Gene LocusTag Fraction L-Asparagine (C) remove 462,648 + Pf6N2E2_394 0.47 -0.2 462,720 + Pf6N2E2_394 0.56 +1.3 462,721 - Pf6N2E2_394 0.56 -0.6 462,721 - Pf6N2E2_394 0.56 +0.2 462,721 - Pf6N2E2_394 0.56 -0.6 462,731 - Pf6N2E2_394 0.58 -0.5 462,760 + Pf6N2E2_394 0.61 +0.8 462,761 - Pf6N2E2_394 0.62 -0.0 462,882 + Pf6N2E2_394 0.77 -1.0 462,890 - Pf6N2E2_394 0.78 +1.4 463,008 + +1.9 463,009 - +0.8 463,009 - -0.0 463,082 + +1.5 463,082 + -0.8 463,082 + +0.4 463,083 - -0.1 463,083 - -0.2 463,083 - +0.5 463,083 - +2.1 463,170 - -0.6 463,184 + -0.4 463,271 + +0.5 463,271 + -0.5 463,272 - -0.1 463,272 - +0.3 463,435 - +0.3 463,558 + Pf6N2E2_395 0.37 -0.9 463,602 + Pf6N2E2_395 0.47 -0.0 463,603 - Pf6N2E2_395 0.48 -0.5 463,697 + Pf6N2E2_395 0.69 -0.1 463,698 - Pf6N2E2_395 0.70 -0.7 463,731 - Pf6N2E2_395 0.77 -1.0 463,795 - -2.1 463,795 - +1.1 463,882 - -0.7 463,924 - -0.3 463,957 - +1.2 464,009 - +1.1 464,009 - +0.0 464,475 + -0.3 464,476 - +1.0 464,508 - -0.3 464,508 - -0.0 464,508 - +0.8 464,508 - -1.3 464,511 + +0.5 464,511 + +0.7 464,511 + +1.8 464,511 + +1.3 464,512 - +0.9 464,512 - -2.0 464,532 + +0.2 464,532 + -2.0 464,533 - +0.2 464,533 - -0.2 464,594 - Pf6N2E2_396 0.20 +0.2 464,594 - Pf6N2E2_396 0.20 +0.1 464,594 - Pf6N2E2_396 0.20 -0.5 464,690 + Pf6N2E2_396 0.43 +0.9 464,719 - Pf6N2E2_396 0.50 +0.2 464,719 - Pf6N2E2_396 0.50 +1.0 464,812 + Pf6N2E2_396 0.73 +0.5 464,812 + Pf6N2E2_396 0.73 +0.1 464,812 + Pf6N2E2_396 0.73 +0.0
Or see this region's nucleotide sequence