Experiment: L-Asparagine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_369 and Pf6N2E2_370 are separated by 131 nucleotides Pf6N2E2_370 and Pf6N2E2_371 are separated by 41 nucleotides
Pf6N2E2_369 - Tartrate dehydrogenase (EC 1.1.1.93) @ Tartrate decarboxylase (EC 4.1.1.73) @ D-malic enzyme (EC 1.1.1.83), at 436,538 to 437,617
_369
Pf6N2E2_370 - Predicted enzyme of the cupin superfamily, at 437,749 to 438,456
_370
Pf6N2E2_371 - Histone acetyltransferase HPA2 and related acetyltransferases, at 438,498 to 439,355
_371
Position (kb) 437
438
439 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 436.754 kb on + strand, within Pf6N2E2_369 at 436.844 kb on + strand, within Pf6N2E2_369 at 436.844 kb on + strand, within Pf6N2E2_369 at 436.845 kb on - strand, within Pf6N2E2_369 at 436.955 kb on + strand, within Pf6N2E2_369 at 437.405 kb on + strand, within Pf6N2E2_369 at 437.405 kb on + strand, within Pf6N2E2_369 at 437.405 kb on + strand, within Pf6N2E2_369 at 437.405 kb on + strand, within Pf6N2E2_369 at 437.406 kb on - strand, within Pf6N2E2_369 at 437.490 kb on - strand, within Pf6N2E2_369 at 437.740 kb on + strand at 437.751 kb on - strand at 437.763 kb on - strand at 437.763 kb on - strand at 437.777 kb on + strand at 437.777 kb on + strand at 437.777 kb on + strand at 437.777 kb on + strand at 437.777 kb on + strand at 437.778 kb on - strand at 437.821 kb on + strand, within Pf6N2E2_370 at 437.869 kb on + strand, within Pf6N2E2_370 at 437.869 kb on + strand, within Pf6N2E2_370 at 437.937 kb on - strand, within Pf6N2E2_370 at 437.937 kb on - strand, within Pf6N2E2_370 at 437.956 kb on - strand, within Pf6N2E2_370 at 437.956 kb on - strand, within Pf6N2E2_370 at 438.238 kb on + strand, within Pf6N2E2_370 at 438.345 kb on - strand, within Pf6N2E2_370 at 438.685 kb on - strand, within Pf6N2E2_371 at 438.984 kb on - strand, within Pf6N2E2_371 at 439.015 kb on + strand, within Pf6N2E2_371 at 439.015 kb on + strand, within Pf6N2E2_371 at 439.016 kb on - strand, within Pf6N2E2_371 at 439.016 kb on - strand, within Pf6N2E2_371 at 439.016 kb on - strand, within Pf6N2E2_371 at 439.117 kb on + strand, within Pf6N2E2_371 at 439.118 kb on - strand, within Pf6N2E2_371 at 439.325 kb on - strand at 439.330 kb on + strand at 439.330 kb on + strand at 439.330 kb on + strand at 439.330 kb on + strand at 439.330 kb on + strand at 439.331 kb on - strand at 439.331 kb on - strand at 439.331 kb on - strand at 439.373 kb on + strand at 439.415 kb on + strand at 439.416 kb on - strand at 439.416 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Asparagine (C) remove 436,754 + Pf6N2E2_369 0.20 +0.2 436,844 + Pf6N2E2_369 0.28 +0.5 436,844 + Pf6N2E2_369 0.28 -0.4 436,845 - Pf6N2E2_369 0.28 +1.4 436,955 + Pf6N2E2_369 0.39 +0.2 437,405 + Pf6N2E2_369 0.80 +0.7 437,405 + Pf6N2E2_369 0.80 +0.0 437,405 + Pf6N2E2_369 0.80 +0.5 437,405 + Pf6N2E2_369 0.80 -0.0 437,406 - Pf6N2E2_369 0.80 +0.2 437,490 - Pf6N2E2_369 0.88 -0.1 437,740 + +0.4 437,751 - +0.0 437,763 - +0.0 437,763 - +0.5 437,777 + +0.3 437,777 + -0.8 437,777 + +1.4 437,777 + -0.6 437,777 + -0.2 437,778 - +0.5 437,821 + Pf6N2E2_370 0.10 -2.3 437,869 + Pf6N2E2_370 0.17 +0.3 437,869 + Pf6N2E2_370 0.17 -2.9 437,937 - Pf6N2E2_370 0.27 -0.4 437,937 - Pf6N2E2_370 0.27 -0.1 437,956 - Pf6N2E2_370 0.29 -0.7 437,956 - Pf6N2E2_370 0.29 -0.4 438,238 + Pf6N2E2_370 0.69 -0.6 438,345 - Pf6N2E2_370 0.84 -0.9 438,685 - Pf6N2E2_371 0.22 -0.1 438,984 - Pf6N2E2_371 0.57 -0.0 439,015 + Pf6N2E2_371 0.60 -0.8 439,015 + Pf6N2E2_371 0.60 +0.2 439,016 - Pf6N2E2_371 0.60 -0.2 439,016 - Pf6N2E2_371 0.60 +0.2 439,016 - Pf6N2E2_371 0.60 +0.7 439,117 + Pf6N2E2_371 0.72 +0.5 439,118 - Pf6N2E2_371 0.72 -0.1 439,325 - -0.4 439,330 + -0.5 439,330 + -1.5 439,330 + +0.5 439,330 + -0.8 439,330 + +0.9 439,331 - +0.2 439,331 - +0.1 439,331 - -0.2 439,373 + +0.2 439,415 + +0.5 439,416 - +0.4 439,416 - +0.5
Or see this region's nucleotide sequence