Experiment: L-Asparagine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1005 and Pf6N2E2_1006 are separated by 486 nucleotides Pf6N2E2_1006 and Pf6N2E2_1007 are separated by 55 nucleotides Pf6N2E2_1007 and Pf6N2E2_1008 are separated by 3 nucleotides
Pf6N2E2_1005 - Ribose ABC transport system, periplasmic ribose-binding protein RbsB (TC 3.A.1.2.1), at 1,174,628 to 1,175,581
_1005
Pf6N2E2_1006 - Ribose ABC transport system, periplasmic ribose-binding protein RbsB (TC 3.A.1.2.1), at 1,176,068 to 1,176,601
_1006
Pf6N2E2_1007 - hypothetical protein, at 1,176,657 to 1,176,788
_1007
Pf6N2E2_1008 - Inositol transport system ATP-binding protein, at 1,176,792 to 1,178,249
_1008
Position (kb)
1176
1177 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1175.285 kb on - strand, within Pf6N2E2_1005 at 1175.564 kb on + strand at 1175.565 kb on - strand at 1175.565 kb on - strand at 1175.593 kb on + strand at 1175.621 kb on + strand at 1175.621 kb on + strand at 1175.621 kb on + strand at 1175.621 kb on + strand at 1175.621 kb on + strand at 1175.621 kb on + strand at 1175.621 kb on + strand at 1175.621 kb on + strand at 1175.622 kb on - strand at 1175.625 kb on + strand at 1175.626 kb on - strand at 1175.676 kb on + strand at 1175.813 kb on - strand at 1175.916 kb on + strand at 1175.964 kb on + strand at 1175.964 kb on + strand at 1175.964 kb on + strand at 1175.965 kb on - strand at 1175.965 kb on - strand at 1176.538 kb on + strand, within Pf6N2E2_1006 at 1176.538 kb on + strand, within Pf6N2E2_1006 at 1176.538 kb on + strand, within Pf6N2E2_1006 at 1176.538 kb on + strand, within Pf6N2E2_1006 at 1176.538 kb on + strand, within Pf6N2E2_1006 at 1176.539 kb on - strand, within Pf6N2E2_1006 at 1176.599 kb on + strand at 1176.599 kb on + strand at 1176.599 kb on + strand at 1176.599 kb on + strand at 1176.599 kb on + strand at 1176.606 kb on + strand at 1176.606 kb on + strand at 1176.829 kb on + strand at 1176.919 kb on + strand at 1176.919 kb on + strand at 1176.934 kb on + strand at 1176.944 kb on + strand, within Pf6N2E2_1008 at 1176.944 kb on + strand, within Pf6N2E2_1008 at 1176.945 kb on - strand, within Pf6N2E2_1008 at 1176.951 kb on + strand, within Pf6N2E2_1008 at 1176.968 kb on + strand, within Pf6N2E2_1008 at 1176.969 kb on - strand, within Pf6N2E2_1008 at 1176.995 kb on + strand, within Pf6N2E2_1008 at 1176.995 kb on + strand, within Pf6N2E2_1008 at 1176.995 kb on + strand, within Pf6N2E2_1008 at 1176.999 kb on + strand, within Pf6N2E2_1008 at 1177.000 kb on - strand, within Pf6N2E2_1008 at 1177.018 kb on + strand, within Pf6N2E2_1008 at 1177.018 kb on + strand, within Pf6N2E2_1008 at 1177.095 kb on - strand, within Pf6N2E2_1008 at 1177.199 kb on + strand, within Pf6N2E2_1008 at 1177.200 kb on - strand, within Pf6N2E2_1008 at 1177.200 kb on - strand, within Pf6N2E2_1008 at 1177.281 kb on + strand, within Pf6N2E2_1008 at 1177.281 kb on + strand, within Pf6N2E2_1008 at 1177.281 kb on + strand, within Pf6N2E2_1008 at 1177.281 kb on + strand, within Pf6N2E2_1008 at 1177.281 kb on + strand, within Pf6N2E2_1008 at 1177.281 kb on + strand, within Pf6N2E2_1008 at 1177.282 kb on - strand, within Pf6N2E2_1008 at 1177.282 kb on - strand, within Pf6N2E2_1008 at 1177.328 kb on + strand, within Pf6N2E2_1008 at 1177.350 kb on + strand, within Pf6N2E2_1008 at 1177.350 kb on + strand, within Pf6N2E2_1008 at 1177.350 kb on + strand, within Pf6N2E2_1008 at 1177.350 kb on + strand, within Pf6N2E2_1008 at 1177.351 kb on - strand, within Pf6N2E2_1008 at 1177.351 kb on - strand, within Pf6N2E2_1008 at 1177.351 kb on - strand, within Pf6N2E2_1008
Per-strain Table
Position Strand Gene LocusTag Fraction L-Asparagine (C) remove 1,175,285 - Pf6N2E2_1005 0.69 +0.4 1,175,564 + +0.7 1,175,565 - -1.2 1,175,565 - -0.4 1,175,593 + -1.1 1,175,621 + -0.4 1,175,621 + +0.0 1,175,621 + -0.9 1,175,621 + +0.4 1,175,621 + +0.4 1,175,621 + -0.4 1,175,621 + +0.9 1,175,621 + -0.9 1,175,622 - +2.7 1,175,625 + +0.2 1,175,626 - +0.4 1,175,676 + -0.6 1,175,813 - +0.3 1,175,916 + +0.3 1,175,964 + -0.4 1,175,964 + +0.4 1,175,964 + -0.3 1,175,965 - +0.4 1,175,965 - +0.6 1,176,538 + Pf6N2E2_1006 0.88 -1.7 1,176,538 + Pf6N2E2_1006 0.88 -0.6 1,176,538 + Pf6N2E2_1006 0.88 +0.4 1,176,538 + Pf6N2E2_1006 0.88 +0.6 1,176,538 + Pf6N2E2_1006 0.88 -1.4 1,176,539 - Pf6N2E2_1006 0.88 -2.9 1,176,599 + +0.4 1,176,599 + +0.5 1,176,599 + -1.8 1,176,599 + -1.0 1,176,599 + -0.6 1,176,606 + -0.6 1,176,606 + -0.9 1,176,829 + -0.4 1,176,919 + +1.0 1,176,919 + -0.1 1,176,934 + +0.2 1,176,944 + Pf6N2E2_1008 0.10 -0.4 1,176,944 + Pf6N2E2_1008 0.10 -0.1 1,176,945 - Pf6N2E2_1008 0.10 -0.9 1,176,951 + Pf6N2E2_1008 0.11 +0.2 1,176,968 + Pf6N2E2_1008 0.12 -1.0 1,176,969 - Pf6N2E2_1008 0.12 -1.7 1,176,995 + Pf6N2E2_1008 0.14 -0.1 1,176,995 + Pf6N2E2_1008 0.14 -0.1 1,176,995 + Pf6N2E2_1008 0.14 -0.2 1,176,999 + Pf6N2E2_1008 0.14 -0.5 1,177,000 - Pf6N2E2_1008 0.14 +1.6 1,177,018 + Pf6N2E2_1008 0.16 +1.1 1,177,018 + Pf6N2E2_1008 0.16 +1.8 1,177,095 - Pf6N2E2_1008 0.21 +0.4 1,177,199 + Pf6N2E2_1008 0.28 -0.0 1,177,200 - Pf6N2E2_1008 0.28 -0.9 1,177,200 - Pf6N2E2_1008 0.28 -1.2 1,177,281 + Pf6N2E2_1008 0.34 +1.0 1,177,281 + Pf6N2E2_1008 0.34 -0.2 1,177,281 + Pf6N2E2_1008 0.34 -0.5 1,177,281 + Pf6N2E2_1008 0.34 +1.5 1,177,281 + Pf6N2E2_1008 0.34 +0.6 1,177,281 + Pf6N2E2_1008 0.34 +0.4 1,177,282 - Pf6N2E2_1008 0.34 +0.7 1,177,282 - Pf6N2E2_1008 0.34 +1.0 1,177,328 + Pf6N2E2_1008 0.37 +0.4 1,177,350 + Pf6N2E2_1008 0.38 -0.2 1,177,350 + Pf6N2E2_1008 0.38 +0.5 1,177,350 + Pf6N2E2_1008 0.38 +0.8 1,177,350 + Pf6N2E2_1008 0.38 -0.4 1,177,351 - Pf6N2E2_1008 0.38 -0.0 1,177,351 - Pf6N2E2_1008 0.38 -0.3 1,177,351 - Pf6N2E2_1008 0.38 -0.4
Or see this region's nucleotide sequence