Experiment: Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 1x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS08040 and BWI76_RS08045 are separated by 3 nucleotides BWI76_RS08045 and BWI76_RS08050 are separated by 17 nucleotides BWI76_RS08050 and BWI76_RS08055 overlap by 68 nucleotides BWI76_RS08055 and BWI76_RS08060 are separated by 46 nucleotides BWI76_RS08060 and BWI76_RS08065 overlap by 8 nucleotides
BWI76_RS08040: BWI76_RS08040 - 23S rRNA (pseudouridine(1915)-N(3))-methyltransferase RlmH, at 1,705,066 to 1,705,533
_RS08040
BWI76_RS08045: BWI76_RS08045 - ribosome silencing factor RsfS, at 1,705,537 to 1,705,854
_RS08045
BWI76_RS08050: BWI76_RS08050 - hypothetical protein, at 1,705,872 to 1,706,143
_RS08050
BWI76_RS08055: BWI76_RS08055 - alpha-ribazole phosphatase, at 1,706,076 to 1,706,705
_RS08055
BWI76_RS08060: BWI76_RS08060 - nicotinic acid mononucleotide adenylyltransferase, at 1,706,752 to 1,707,402
_RS08060
BWI76_RS08065: BWI76_RS08065 - DNA polymerase III subunit delta, at 1,707,395 to 1,708,426
_RS08065
Position (kb)
1706
1707 Strain fitness (log2 ratio)
-1
0
1 at 1705.159 kb on - strand, within BWI76_RS08040 at 1705.341 kb on - strand, within BWI76_RS08040 at 1706.276 kb on - strand, within BWI76_RS08055 at 1706.727 kb on + strand at 1706.735 kb on - strand at 1706.735 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 1x remove 1,705,159 - BWI76_RS08040 0.20 -0.5 1,705,341 - BWI76_RS08040 0.59 +0.4 1,706,276 - BWI76_RS08055 0.32 -0.3 1,706,727 + +0.6 1,706,735 - +1.1 1,706,735 - -0.0
Or see this region's nucleotide sequence