Experiment: BHIS with Trimethoprim 0.0125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT4745 and BT4746 are separated by 558 nucleotides BT4746 and BT4747 are separated by 5 nucleotides BT4747 and BT4748 are separated by 162 nucleotides
BT4745: BT4745 - conserved hypothetical protein (NCBI ptt file), at 6,225,109 to 6,228,777
BT4745
BT4746: BT4746 - conserved hypothetical protein (NCBI ptt file), at 6,229,336 to 6,229,590
BT4746
BT4747: BT4747 - hypothetical protein (NCBI ptt file), at 6,229,596 to 6,229,925
BT4747
BT4748: BT4748 - hypothetical protein (NCBI ptt file), at 6,230,088 to 6,231,110
BT4748
Position (kb)
6229
6230 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 6228.357 kb on + strand, within BT4745 at 6228.362 kb on - strand, within BT4745 at 6228.444 kb on + strand at 6228.447 kb on + strand at 6228.470 kb on - strand at 6228.483 kb on - strand at 6228.539 kb on + strand at 6228.555 kb on + strand at 6228.660 kb on - strand at 6228.689 kb on + strand at 6228.689 kb on + strand at 6228.699 kb on - strand at 6228.714 kb on + strand at 6228.714 kb on + strand at 6228.716 kb on + strand at 6228.775 kb on + strand at 6228.776 kb on - strand at 6228.796 kb on + strand at 6228.892 kb on + strand at 6228.938 kb on - strand at 6228.940 kb on - strand at 6228.940 kb on - strand at 6228.940 kb on - strand at 6228.994 kb on + strand at 6228.994 kb on + strand at 6229.008 kb on + strand at 6229.018 kb on + strand at 6229.027 kb on + strand at 6229.052 kb on - strand at 6229.074 kb on - strand at 6229.119 kb on + strand at 6229.135 kb on - strand at 6229.300 kb on - strand at 6229.350 kb on - strand at 6229.363 kb on - strand, within BT4746 at 6229.363 kb on - strand, within BT4746 at 6229.395 kb on - strand, within BT4746 at 6229.483 kb on - strand, within BT4746 at 6229.524 kb on - strand, within BT4746 at 6229.526 kb on - strand, within BT4746 at 6229.589 kb on - strand at 6229.589 kb on - strand at 6229.689 kb on - strand, within BT4747 at 6229.689 kb on - strand, within BT4747 at 6229.831 kb on - strand, within BT4747 at 6229.847 kb on + strand, within BT4747 at 6229.928 kb on + strand at 6229.928 kb on + strand at 6230.089 kb on + strand at 6230.259 kb on - strand, within BT4748 at 6230.341 kb on - strand, within BT4748 at 6230.414 kb on + strand at 6230.414 kb on + strand, within BT4748 at 6230.415 kb on - strand, within BT4748 at 6230.486 kb on + strand, within BT4748 at 6230.488 kb on + strand, within BT4748 at 6230.489 kb on - strand, within BT4748 at 6230.489 kb on - strand, within BT4748
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Trimethoprim 0.0125 mM remove 6,228,357 + BT4745 0.89 -1.1 6,228,362 - BT4745 0.89 +1.5 6,228,444 + -1.6 6,228,447 + -3.3 6,228,470 - +0.5 6,228,483 - -0.1 6,228,539 + -2.7 6,228,555 + -1.9 6,228,660 - -1.0 6,228,689 + -2.7 6,228,689 + +1.0 6,228,699 - +0.2 6,228,714 + -3.1 6,228,714 + -0.7 6,228,716 + +1.5 6,228,775 + -2.4 6,228,776 - +1.5 6,228,796 + +2.3 6,228,892 + -0.8 6,228,938 - +2.8 6,228,940 - +0.9 6,228,940 - -1.6 6,228,940 - -1.2 6,228,994 + -2.4 6,228,994 + -1.1 6,229,008 + -0.3 6,229,018 + -3.3 6,229,027 + -1.4 6,229,052 - -1.1 6,229,074 - -1.7 6,229,119 + -0.2 6,229,135 - -1.4 6,229,300 - +1.6 6,229,350 - -2.1 6,229,363 - BT4746 0.11 -0.5 6,229,363 - BT4746 0.11 +1.8 6,229,395 - BT4746 0.23 +2.6 6,229,483 - BT4746 0.58 +2.7 6,229,524 - BT4746 0.74 +2.7 6,229,526 - BT4746 0.75 +0.9 6,229,589 - -1.0 6,229,589 - +1.5 6,229,689 - BT4747 0.28 +0.2 6,229,689 - BT4747 0.28 -1.7 6,229,831 - BT4747 0.71 -1.1 6,229,847 + BT4747 0.76 -1.8 6,229,928 + -1.8 6,229,928 + +1.9 6,230,089 + +1.5 6,230,259 - BT4748 0.17 -2.1 6,230,341 - BT4748 0.25 +2.4 6,230,414 + -2.1 6,230,414 + BT4748 0.32 +1.1 6,230,415 - BT4748 0.32 +3.1 6,230,486 + BT4748 0.39 +0.6 6,230,488 + BT4748 0.39 -2.4 6,230,489 - BT4748 0.39 -3.6 6,230,489 - BT4748 0.39 -0.7
Or see this region's nucleotide sequence