Strain Fitness in Bacteroides thetaiotaomicron VPI-5482 around BT4447

Experiment: BHIS with Trimethoprim 0.0125 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBT4445 and BT4446 are separated by 73 nucleotidesBT4446 and BT4447 overlap by 1 nucleotidesBT4447 and BT4448 are separated by 187 nucleotides BT4445: BT4445 - conserved hypothetical protein (NCBI ptt file), at 5,855,714 to 5,857,429 BT4445 BT4446: BT4446 - sugar phosphate isomerase/epimerase (NCBI ptt file), at 5,857,503 to 5,858,390 BT4446 BT4447: BT4447 - conserved hypothetical protein (NCBI ptt file), at 5,858,390 to 5,859,112 BT4447 BT4448: BT4448 - putative dehydrogenase (NCBI ptt file), at 5,859,300 to 5,860,775 BT4448 Position (kb) 5858 5859 5860Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3at 5857.424 kb on + strandat 5857.425 kb on - strandat 5857.428 kb on - strandat 5857.463 kb on + strandat 5857.498 kb on + strandat 5857.499 kb on - strandat 5857.509 kb on - strandat 5857.509 kb on - strandat 5857.513 kb on + strandat 5857.514 kb on - strandat 5857.547 kb on - strandat 5857.576 kb on - strandat 5857.588 kb on + strandat 5857.597 kb on - strand, within BT4446at 5857.610 kb on + strand, within BT4446at 5857.610 kb on + strand, within BT4446at 5857.611 kb on - strand, within BT4446at 5857.611 kb on - strand, within BT4446at 5857.618 kb on - strand, within BT4446at 5857.647 kb on - strand, within BT4446at 5857.647 kb on - strand, within BT4446at 5857.665 kb on - strand, within BT4446at 5857.665 kb on - strand, within BT4446at 5857.670 kb on + strand, within BT4446at 5857.774 kb on - strand, within BT4446at 5857.774 kb on - strand, within BT4446at 5857.796 kb on - strand, within BT4446at 5857.820 kb on + strand, within BT4446at 5857.822 kb on + strand, within BT4446at 5857.822 kb on + strand, within BT4446at 5857.823 kb on - strand, within BT4446at 5857.875 kb on - strand, within BT4446at 5857.986 kb on + strand, within BT4446at 5857.994 kb on - strand, within BT4446at 5858.012 kb on + strand, within BT4446at 5858.112 kb on + strand, within BT4446at 5858.113 kb on - strand, within BT4446at 5858.145 kb on - strand, within BT4446at 5858.185 kb on + strand, within BT4446at 5858.210 kb on - strand, within BT4446at 5858.239 kb on - strand, within BT4446at 5858.263 kb on - strand, within BT4446at 5858.290 kb on - strand, within BT4446at 5858.361 kb on + strandat 5858.361 kb on + strandat 5858.366 kb on - strandat 5858.391 kb on + strandat 5858.427 kb on - strandat 5858.621 kb on - strand, within BT4447at 5858.825 kb on - strand, within BT4447at 5858.984 kb on - strand, within BT4447at 5858.984 kb on - strand, within BT4447at 5859.089 kb on + strandat 5859.262 kb on + strandat 5859.311 kb on + strandat 5859.337 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction BHIS with Trimethoprim 0.0125 mM
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5,857,424 + +0.7
5,857,425 - -2.4
5,857,428 - -1.6
5,857,463 + -0.3
5,857,498 + -0.4
5,857,499 - +2.0
5,857,509 - -1.0
5,857,509 - -2.6
5,857,513 + -1.1
5,857,514 - -1.8
5,857,547 - -1.1
5,857,576 - -0.6
5,857,588 + -0.0
5,857,597 - BT4446 0.11 -0.0
5,857,610 + BT4446 0.12 -1.0
5,857,610 + BT4446 0.12 +0.5
5,857,611 - BT4446 0.12 +0.1
5,857,611 - BT4446 0.12 -1.7
5,857,618 - BT4446 0.13 -0.3
5,857,647 - BT4446 0.16 +1.6
5,857,647 - BT4446 0.16 +1.6
5,857,665 - BT4446 0.18 -0.6
5,857,665 - BT4446 0.18 +1.9
5,857,670 + BT4446 0.19 -2.3
5,857,774 - BT4446 0.31 -1.7
5,857,774 - BT4446 0.31 -0.4
5,857,796 - BT4446 0.33 -3.8
5,857,820 + BT4446 0.36 +2.8
5,857,822 + BT4446 0.36 -1.7
5,857,822 + BT4446 0.36 +1.6
5,857,823 - BT4446 0.36 -1.0
5,857,875 - BT4446 0.42 +1.5
5,857,986 + BT4446 0.54 -1.0
5,857,994 - BT4446 0.55 -3.6
5,858,012 + BT4446 0.57 -2.6
5,858,112 + BT4446 0.69 -1.1
5,858,113 - BT4446 0.69 -1.5
5,858,145 - BT4446 0.72 +2.0
5,858,185 + BT4446 0.77 +1.3
5,858,210 - BT4446 0.80 -0.0
5,858,239 - BT4446 0.83 +2.6
5,858,263 - BT4446 0.86 -2.7
5,858,290 - BT4446 0.89 -0.0
5,858,361 + -0.6
5,858,361 + +2.2
5,858,366 - -2.3
5,858,391 + -3.0
5,858,427 - -2.8
5,858,621 - BT4447 0.32 -1.9
5,858,825 - BT4447 0.60 -1.7
5,858,984 - BT4447 0.82 +1.3
5,858,984 - BT4447 0.82 -3.0
5,859,089 + -0.0
5,859,262 + -1.4
5,859,311 + -0.0
5,859,337 + -0.6

Or see this region's nucleotide sequence