Experiment: BHIS with Trimethoprim 0.0125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT4332 and BT4333 are separated by 6 nucleotides BT4333 and BT4334 are separated by 122 nucleotides BT4334 and BT4335 are separated by 19 nucleotides BT4335 and BT4336 are separated by 60 nucleotides
BT4332: BT4332 - conserved hypothetical protein (NCBI ptt file), at 5,705,567 to 5,706,217
BT4332
BT4333: BT4333 - hypothetical protein (NCBI ptt file), at 5,706,224 to 5,706,883
BT4333
BT4334: BT4334 - FtsK/SpoIIIE family protein (NCBI ptt file), at 5,707,006 to 5,709,501
BT4334
BT4335: BT4335 - conserved hypothetical protein (NCBI ptt file), at 5,709,521 to 5,710,168
BT4335
BT4336: BT4336 - thioredoxin reductase (NCBI ptt file), at 5,710,229 to 5,711,179
BT4336
Position (kb) 5707
5708
5709
5710 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1
2
3 at 5706.026 kb on + strand, within BT4332 at 5706.026 kb on + strand, within BT4332 at 5706.026 kb on + strand, within BT4332 at 5706.031 kb on + strand, within BT4332 at 5706.032 kb on - strand, within BT4332 at 5706.107 kb on - strand, within BT4332 at 5706.108 kb on - strand, within BT4332 at 5706.161 kb on + strand at 5706.182 kb on + strand at 5706.183 kb on - strand at 5706.209 kb on + strand at 5706.226 kb on - strand at 5706.309 kb on + strand, within BT4333 at 5706.332 kb on - strand, within BT4333 at 5706.376 kb on + strand, within BT4333 at 5706.410 kb on + strand, within BT4333 at 5706.505 kb on + strand, within BT4333 at 5706.517 kb on + strand, within BT4333 at 5706.576 kb on + strand, within BT4333 at 5706.583 kb on + strand, within BT4333 at 5706.606 kb on + strand, within BT4333 at 5706.606 kb on + strand, within BT4333 at 5706.606 kb on + strand, within BT4333 at 5706.606 kb on + strand, within BT4333 at 5706.837 kb on - strand at 5706.860 kb on + strand at 5706.910 kb on + strand at 5706.967 kb on + strand at 5710.161 kb on + strand at 5710.161 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Trimethoprim 0.0125 mM remove 5,706,026 + BT4332 0.71 +1.0 5,706,026 + BT4332 0.71 -3.9 5,706,026 + BT4332 0.71 -4.4 5,706,031 + BT4332 0.71 +1.6 5,706,032 - BT4332 0.71 +1.0 5,706,107 - BT4332 0.83 -1.0 5,706,108 - BT4332 0.83 +0.0 5,706,161 + -3.5 5,706,182 + +0.0 5,706,183 - -0.1 5,706,209 + +1.6 5,706,226 - -1.2 5,706,309 + BT4333 0.13 -0.6 5,706,332 - BT4333 0.16 +1.4 5,706,376 + BT4333 0.23 -4.8 5,706,410 + BT4333 0.28 -2.6 5,706,505 + BT4333 0.43 -2.0 5,706,517 + BT4333 0.44 +1.0 5,706,576 + BT4333 0.53 +0.6 5,706,583 + BT4333 0.54 -1.1 5,706,606 + BT4333 0.58 -4.2 5,706,606 + BT4333 0.58 -0.4 5,706,606 + BT4333 0.58 -3.3 5,706,606 + BT4333 0.58 -2.7 5,706,837 - +1.9 5,706,860 + -3.8 5,706,910 + -6.7 5,706,967 + -1.6 5,710,161 + -1.6 5,710,161 + +3.5
Or see this region's nucleotide sequence