Experiment: BHIS with Trimethoprim 0.0125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT4328 and BT4329 are separated by 6 nucleotides BT4329 and BT4330 are separated by 13 nucleotides BT4330 and BT4331 are separated by 376 nucleotides
BT4328: BT4328 - conserved hypothetical protein (NCBI ptt file), at 5,701,392 to 5,702,090
BT4328
BT4329: BT4329 - conserved hypothetical protein with a conserved domain (NCBI ptt file), at 5,702,097 to 5,702,693
BT4329
BT4330: BT4330 - xanthosine permease (NCBI ptt file), at 5,702,707 to 5,703,957
BT4330
BT4331: BT4331 - conserved hypothetical protein (NCBI ptt file), at 5,704,334 to 5,705,512
BT4331
Position (kb)
5702
5703
5704 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1
2 at 5701.957 kb on + strand, within BT4328 at 5701.958 kb on - strand, within BT4328 at 5701.980 kb on - strand, within BT4328 at 5702.090 kb on + strand at 5702.205 kb on - strand, within BT4329 at 5702.372 kb on + strand, within BT4329 at 5702.378 kb on + strand, within BT4329 at 5702.420 kb on - strand, within BT4329 at 5702.420 kb on - strand, within BT4329 at 5702.433 kb on - strand, within BT4329 at 5702.471 kb on + strand, within BT4329 at 5702.479 kb on - strand, within BT4329 at 5702.482 kb on - strand, within BT4329 at 5702.543 kb on + strand, within BT4329 at 5702.545 kb on + strand, within BT4329 at 5702.577 kb on + strand, within BT4329 at 5702.578 kb on - strand, within BT4329 at 5702.585 kb on + strand, within BT4329 at 5702.586 kb on - strand, within BT4329 at 5702.664 kb on + strand at 5702.664 kb on + strand at 5702.674 kb on - strand at 5702.708 kb on + strand at 5702.713 kb on + strand at 5702.714 kb on - strand at 5702.718 kb on + strand at 5702.793 kb on + strand at 5702.793 kb on + strand at 5702.825 kb on - strand at 5702.825 kb on - strand at 5702.842 kb on + strand, within BT4330 at 5702.842 kb on + strand, within BT4330 at 5703.011 kb on + strand, within BT4330 at 5703.241 kb on - strand, within BT4330 at 5703.273 kb on - strand, within BT4330 at 5703.350 kb on - strand, within BT4330 at 5703.353 kb on - strand, within BT4330 at 5703.434 kb on + strand, within BT4330 at 5703.437 kb on - strand, within BT4330 at 5703.466 kb on + strand, within BT4330 at 5703.509 kb on - strand, within BT4330 at 5703.585 kb on + strand, within BT4330 at 5703.605 kb on + strand, within BT4330 at 5703.606 kb on - strand, within BT4330 at 5703.606 kb on - strand, within BT4330 at 5703.606 kb on - strand, within BT4330 at 5703.606 kb on - strand, within BT4330 at 5703.639 kb on + strand, within BT4330 at 5703.639 kb on + strand, within BT4330 at 5703.639 kb on + strand, within BT4330 at 5703.640 kb on - strand, within BT4330 at 5703.640 kb on - strand, within BT4330 at 5703.640 kb on - strand, within BT4330 at 5703.642 kb on - strand, within BT4330 at 5703.642 kb on - strand, within BT4330 at 5703.642 kb on - strand, within BT4330 at 5703.643 kb on + strand, within BT4330 at 5703.643 kb on + strand, within BT4330 at 5703.644 kb on - strand, within BT4330 at 5703.671 kb on + strand, within BT4330 at 5703.705 kb on - strand, within BT4330 at 5703.705 kb on - strand, within BT4330 at 5703.746 kb on - strand, within BT4330 at 5703.769 kb on - strand, within BT4330 at 5703.876 kb on - strand at 5703.930 kb on + strand at 5703.931 kb on - strand at 5703.949 kb on + strand at 5703.996 kb on + strand at 5703.996 kb on + strand at 5704.044 kb on + strand at 5704.055 kb on + strand at 5704.090 kb on + strand at 5704.090 kb on + strand at 5704.117 kb on + strand at 5704.119 kb on + strand at 5704.172 kb on + strand at 5704.211 kb on - strand at 5704.330 kb on + strand at 5704.331 kb on - strand at 5704.331 kb on - strand at 5704.332 kb on + strand at 5704.332 kb on + strand at 5704.333 kb on - strand at 5704.333 kb on - strand at 5704.333 kb on - strand at 5704.336 kb on - strand at 5704.336 kb on - strand at 5704.340 kb on + strand at 5704.340 kb on + strand at 5704.341 kb on - strand at 5704.341 kb on - strand at 5704.344 kb on + strand at 5704.344 kb on + strand at 5704.344 kb on + strand at 5704.344 kb on + strand at 5704.349 kb on + strand at 5704.349 kb on + strand at 5704.366 kb on - strand at 5704.385 kb on + strand at 5704.387 kb on + strand at 5704.387 kb on + strand at 5704.387 kb on + strand at 5704.433 kb on + strand at 5704.433 kb on + strand at 5704.433 kb on + strand at 5704.433 kb on + strand at 5704.433 kb on + strand at 5704.434 kb on - strand at 5704.434 kb on - strand at 5704.434 kb on - strand at 5704.434 kb on - strand at 5704.434 kb on - strand at 5704.434 kb on - strand at 5704.450 kb on + strand at 5704.450 kb on + strand at 5704.604 kb on - strand, within BT4331 at 5704.644 kb on + strand, within BT4331 at 5704.669 kb on - strand, within BT4331 at 5704.727 kb on + strand, within BT4331 at 5704.728 kb on - strand, within BT4331 at 5704.778 kb on + strand, within BT4331 at 5704.802 kb on - strand, within BT4331 at 5704.806 kb on + strand at 5704.806 kb on + strand, within BT4331 at 5704.806 kb on + strand, within BT4331 at 5704.807 kb on - strand, within BT4331 at 5704.807 kb on - strand, within BT4331 at 5704.807 kb on - strand, within BT4331 at 5704.807 kb on - strand, within BT4331 at 5704.827 kb on + strand, within BT4331 at 5704.828 kb on - strand, within BT4331 at 5704.837 kb on + strand, within BT4331 at 5704.837 kb on + strand, within BT4331 at 5704.838 kb on - strand, within BT4331 at 5704.863 kb on + strand, within BT4331 at 5704.864 kb on - strand, within BT4331 at 5704.934 kb on + strand, within BT4331 at 5704.935 kb on - strand, within BT4331
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Trimethoprim 0.0125 mM remove 5,701,957 + BT4328 0.81 -4.2 5,701,958 - BT4328 0.81 -2.3 5,701,980 - BT4328 0.84 -0.7 5,702,090 + +1.0 5,702,205 - BT4329 0.18 -1.0 5,702,372 + BT4329 0.46 -2.5 5,702,378 + BT4329 0.47 -2.6 5,702,420 - BT4329 0.54 +2.0 5,702,420 - BT4329 0.54 -2.8 5,702,433 - BT4329 0.56 -2.3 5,702,471 + BT4329 0.63 -2.0 5,702,479 - BT4329 0.64 +1.2 5,702,482 - BT4329 0.64 +2.0 5,702,543 + BT4329 0.75 -1.9 5,702,545 + BT4329 0.75 -1.2 5,702,577 + BT4329 0.80 -1.0 5,702,578 - BT4329 0.81 -0.5 5,702,585 + BT4329 0.82 -2.7 5,702,586 - BT4329 0.82 +0.0 5,702,664 + -0.0 5,702,664 + -4.6 5,702,674 - -1.0 5,702,708 + -3.4 5,702,713 + -4.2 5,702,714 - +0.0 5,702,718 + -3.4 5,702,793 + -2.0 5,702,793 + -1.0 5,702,825 - -2.3 5,702,825 - -2.3 5,702,842 + BT4330 0.11 -2.6 5,702,842 + BT4330 0.11 -3.8 5,703,011 + BT4330 0.24 -2.8 5,703,241 - BT4330 0.43 -2.6 5,703,273 - BT4330 0.45 -5.6 5,703,350 - BT4330 0.51 -2.0 5,703,353 - BT4330 0.52 -2.8 5,703,434 + BT4330 0.58 -4.8 5,703,437 - BT4330 0.58 -3.4 5,703,466 + BT4330 0.61 -1.6 5,703,509 - BT4330 0.64 -0.6 5,703,585 + BT4330 0.70 +0.0 5,703,605 + BT4330 0.72 -4.5 5,703,606 - BT4330 0.72 +0.0 5,703,606 - BT4330 0.72 -3.9 5,703,606 - BT4330 0.72 -3.2 5,703,606 - BT4330 0.72 -2.6 5,703,639 + BT4330 0.75 -2.0 5,703,639 + BT4330 0.75 -2.0 5,703,639 + BT4330 0.75 -1.6 5,703,640 - BT4330 0.75 -1.6 5,703,640 - BT4330 0.75 -4.6 5,703,640 - BT4330 0.75 -2.3 5,703,642 - BT4330 0.75 -4.4 5,703,642 - BT4330 0.75 -5.2 5,703,642 - BT4330 0.75 -4.4 5,703,643 + BT4330 0.75 -2.8 5,703,643 + BT4330 0.75 -2.8 5,703,644 - BT4330 0.75 -2.6 5,703,671 + BT4330 0.77 -2.3 5,703,705 - BT4330 0.80 -2.3 5,703,705 - BT4330 0.80 -2.0 5,703,746 - BT4330 0.83 -3.9 5,703,769 - BT4330 0.85 -2.0 5,703,876 - -4.4 5,703,930 + -4.6 5,703,931 - -4.1 5,703,949 + -5.2 5,703,996 + -4.3 5,703,996 + -2.3 5,704,044 + -3.8 5,704,055 + -5.9 5,704,090 + -5.1 5,704,090 + -5.5 5,704,117 + -4.0 5,704,119 + -1.2 5,704,172 + -1.0 5,704,211 - -1.6 5,704,330 + -2.3 5,704,331 - +1.0 5,704,331 - -2.0 5,704,332 + +0.0 5,704,332 + -4.5 5,704,333 - -2.3 5,704,333 - -1.0 5,704,333 - -1.0 5,704,336 - -2.8 5,704,336 - -2.3 5,704,340 + -4.2 5,704,340 + -3.2 5,704,341 - -1.6 5,704,341 - -3.3 5,704,344 + +0.0 5,704,344 + +0.0 5,704,344 + -0.4 5,704,344 + -3.8 5,704,349 + -1.3 5,704,349 + -4.4 5,704,366 - -3.4 5,704,385 + +0.0 5,704,387 + -3.3 5,704,387 + -3.3 5,704,387 + -3.6 5,704,433 + -2.2 5,704,433 + +0.3 5,704,433 + -4.4 5,704,433 + -5.0 5,704,433 + -5.0 5,704,434 - -3.6 5,704,434 - -4.4 5,704,434 - -3.3 5,704,434 - -2.8 5,704,434 - +1.7 5,704,434 - -2.3 5,704,450 + -3.3 5,704,450 + -3.7 5,704,604 - BT4331 0.23 -1.2 5,704,644 + BT4331 0.26 -3.8 5,704,669 - BT4331 0.28 -3.1 5,704,727 + BT4331 0.33 +0.1 5,704,728 - BT4331 0.33 -4.2 5,704,778 + BT4331 0.38 -1.0 5,704,802 - BT4331 0.40 -3.2 5,704,806 + +0.0 5,704,806 + BT4331 0.40 -2.0 5,704,806 + BT4331 0.40 -2.9 5,704,807 - BT4331 0.40 -3.2 5,704,807 - BT4331 0.40 -3.2 5,704,807 - BT4331 0.40 -3.3 5,704,807 - BT4331 0.40 -2.6 5,704,827 + BT4331 0.42 -5.2 5,704,828 - BT4331 0.42 -3.8 5,704,837 + BT4331 0.43 -5.8 5,704,837 + BT4331 0.43 -4.1 5,704,838 - BT4331 0.43 -2.5 5,704,863 + BT4331 0.45 -4.1 5,704,864 - BT4331 0.45 -0.6 5,704,934 + BT4331 0.51 +0.6 5,704,935 - BT4331 0.51 -3.2
Or see this region's nucleotide sequence