Strain Fitness in Bacteroides thetaiotaomicron VPI-5482 around BT4211

Experiment: BHIS with Trimethoprim 0.0125 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBT4210 and BT4211 are separated by 3 nucleotidesBT4211 and BT4212 are separated by 325 nucleotidesBT4212 and BT4213 are separated by 50 nucleotides BT4210: BT4210 - peptide chain release factor 1 (NCBI ptt file), at 5,546,853 to 5,547,965 BT4210 BT4211: BT4211 - putative phosphoribosylformylglycinamidine cyclo-ligase (NCBI ptt file), at 5,547,969 to 5,549,135 BT4211 BT4212: BT4212 - conserved hypothetical protein (NCBI ptt file), at 5,549,461 to 5,550,042 BT4212 BT4213: BT4213 - conserved hypothetical protein (NCBI ptt file), at 5,550,093 to 5,550,995 BT4213 Position (kb) 5547 5548 5549 5550Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3 4 5 6 7 8at 5548.039 kb on - strandat 5548.042 kb on - strandat 5548.042 kb on - strandat 5548.042 kb on - strandat 5548.138 kb on - strand, within BT4211at 5548.164 kb on - strand, within BT4211at 5548.287 kb on - strand, within BT4211at 5548.306 kb on - strand, within BT4211at 5548.466 kb on - strand, within BT4211at 5548.539 kb on - strand, within BT4211at 5548.553 kb on - strand, within BT4211at 5548.557 kb on - strand, within BT4211at 5548.717 kb on - strand, within BT4211at 5548.717 kb on - strand, within BT4211at 5548.941 kb on - strand, within BT4211at 5549.149 kb on - strandat 5549.240 kb on - strandat 5549.240 kb on - strandat 5549.354 kb on + strandat 5549.397 kb on - strandat 5549.463 kb on - strandat 5549.471 kb on - strandat 5549.471 kb on - strandat 5549.510 kb on + strandat 5549.732 kb on - strand, within BT4212at 5549.814 kb on + strand, within BT4212at 5549.825 kb on - strand, within BT4212at 5549.861 kb on + strand, within BT4212at 5549.870 kb on - strand, within BT4212at 5549.973 kb on + strand, within BT4212at 5549.974 kb on - strand, within BT4212at 5549.974 kb on - strand, within BT4212at 5550.078 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction BHIS with Trimethoprim 0.0125 mM
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5,548,039 - +3.6
5,548,042 - +3.8
5,548,042 - +0.9
5,548,042 - +8.1
5,548,138 - BT4211 0.14 +5.9
5,548,164 - BT4211 0.17 +5.6
5,548,287 - BT4211 0.27 +4.4
5,548,306 - BT4211 0.29 +3.5
5,548,466 - BT4211 0.43 +2.6
5,548,539 - BT4211 0.49 +4.0
5,548,553 - BT4211 0.50 +5.9
5,548,557 - BT4211 0.50 +3.5
5,548,717 - BT4211 0.64 +4.6
5,548,717 - BT4211 0.64 +3.5
5,548,941 - BT4211 0.83 -0.4
5,549,149 - -0.9
5,549,240 - +1.1
5,549,240 - -1.0
5,549,354 + -1.9
5,549,397 - -1.0
5,549,463 - -3.3
5,549,471 - +1.4
5,549,471 - +1.5
5,549,510 + -2.3
5,549,732 - BT4212 0.47 +0.1
5,549,814 + BT4212 0.61 -3.9
5,549,825 - BT4212 0.63 -1.5
5,549,861 + BT4212 0.69 +2.3
5,549,870 - BT4212 0.70 -1.7
5,549,973 + BT4212 0.88 -2.3
5,549,974 - BT4212 0.88 -2.7
5,549,974 - BT4212 0.88 -1.5
5,550,078 + -0.9

Or see this region's nucleotide sequence