Experiment: BHIS with Trimethoprim 0.0125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT4147 and BT4148 overlap by 50 nucleotides BT4148 and BT4149 are separated by 245 nucleotides BT4149 and BT4150 are separated by 24 nucleotides
BT4147: BT4147 - hypothetical protein (NCBI ptt file), at 5,445,658 to 5,446,248
BT4147
BT4148: BT4148 - hypothetical protein (NCBI ptt file), at 5,446,199 to 5,446,414
BT4148
BT4149: BT4149 - exo-poly-alpha-D-galacturonosidase precursor (NCBI ptt file), at 5,446,660 to 5,447,718
BT4149
BT4150: BT4150 - putative rhamnogalacturonan acetylesterase (NCBI ptt file), at 5,447,743 to 5,448,981
BT4150
Position (kb) 5446
5447
5448 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 5445.676 kb on - strand at 5445.717 kb on - strand, within BT4147 at 5445.955 kb on + strand, within BT4147 at 5445.971 kb on - strand, within BT4147 at 5445.972 kb on + strand, within BT4147 at 5446.014 kb on + strand, within BT4147 at 5446.014 kb on + strand, within BT4147 at 5446.150 kb on - strand, within BT4147 at 5446.333 kb on + strand, within BT4148 at 5446.333 kb on + strand, within BT4148 at 5446.345 kb on + strand, within BT4148 at 5446.418 kb on - strand at 5446.452 kb on - strand at 5446.479 kb on + strand at 5446.481 kb on + strand at 5446.482 kb on - strand at 5446.482 kb on - strand at 5446.590 kb on + strand at 5446.590 kb on + strand at 5446.666 kb on - strand at 5446.669 kb on + strand at 5446.721 kb on + strand at 5446.721 kb on + strand at 5446.750 kb on + strand at 5446.753 kb on + strand at 5446.753 kb on + strand at 5446.753 kb on + strand at 5446.861 kb on + strand, within BT4149 at 5446.896 kb on + strand, within BT4149 at 5446.915 kb on + strand, within BT4149 at 5446.936 kb on + strand, within BT4149 at 5447.174 kb on + strand, within BT4149 at 5447.233 kb on - strand, within BT4149 at 5447.311 kb on + strand, within BT4149 at 5447.408 kb on - strand, within BT4149 at 5447.423 kb on + strand, within BT4149 at 5447.506 kb on + strand, within BT4149 at 5447.545 kb on + strand, within BT4149 at 5447.548 kb on - strand, within BT4149 at 5447.718 kb on + strand at 5447.719 kb on - strand at 5447.721 kb on - strand at 5447.728 kb on + strand at 5447.729 kb on - strand at 5447.733 kb on + strand at 5447.800 kb on - strand at 5447.909 kb on + strand, within BT4150 at 5447.934 kb on + strand, within BT4150 at 5447.935 kb on - strand, within BT4150 at 5448.033 kb on + strand, within BT4150 at 5448.033 kb on + strand, within BT4150 at 5448.034 kb on - strand, within BT4150 at 5448.034 kb on - strand, within BT4150 at 5448.236 kb on + strand, within BT4150 at 5448.239 kb on + strand, within BT4150 at 5448.239 kb on + strand, within BT4150 at 5448.239 kb on + strand, within BT4150 at 5448.240 kb on - strand, within BT4150 at 5448.357 kb on + strand, within BT4150 at 5448.471 kb on + strand, within BT4150 at 5448.471 kb on + strand, within BT4150 at 5448.496 kb on - strand, within BT4150 at 5448.660 kb on + strand, within BT4150
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Trimethoprim 0.0125 mM remove 5,445,676 - -2.2 5,445,717 - BT4147 0.10 -1.4 5,445,955 + BT4147 0.50 -2.2 5,445,971 - BT4147 0.53 +1.5 5,445,972 + BT4147 0.53 -3.0 5,446,014 + BT4147 0.60 +3.4 5,446,014 + BT4147 0.60 -0.9 5,446,150 - BT4147 0.83 +0.1 5,446,333 + BT4148 0.62 -0.9 5,446,333 + BT4148 0.62 +1.1 5,446,345 + BT4148 0.68 -2.2 5,446,418 - -0.5 5,446,452 - +0.7 5,446,479 + +3.8 5,446,481 + -0.9 5,446,482 - +2.6 5,446,482 - -2.2 5,446,590 + -2.1 5,446,590 + -3.4 5,446,666 - -0.4 5,446,669 + -1.4 5,446,721 + +1.1 5,446,721 + -1.0 5,446,750 + +0.4 5,446,753 + +2.4 5,446,753 + +0.3 5,446,753 + +1.9 5,446,861 + BT4149 0.19 +2.1 5,446,896 + BT4149 0.22 +1.1 5,446,915 + BT4149 0.24 -2.7 5,446,936 + BT4149 0.26 -2.7 5,447,174 + BT4149 0.49 +1.7 5,447,233 - BT4149 0.54 +2.4 5,447,311 + BT4149 0.61 +2.6 5,447,408 - BT4149 0.71 -0.9 5,447,423 + BT4149 0.72 -0.1 5,447,506 + BT4149 0.80 -2.9 5,447,545 + BT4149 0.84 +1.7 5,447,548 - BT4149 0.84 +1.9 5,447,718 + -2.3 5,447,719 - +1.6 5,447,721 - +0.1 5,447,728 + -0.7 5,447,729 - +0.1 5,447,733 + +0.5 5,447,800 - -1.4 5,447,909 + BT4150 0.13 -1.1 5,447,934 + BT4150 0.15 +2.7 5,447,935 - BT4150 0.15 +3.1 5,448,033 + BT4150 0.23 +0.1 5,448,033 + BT4150 0.23 -1.2 5,448,034 - BT4150 0.23 +0.0 5,448,034 - BT4150 0.23 +4.0 5,448,236 + BT4150 0.40 +3.8 5,448,239 + BT4150 0.40 -2.2 5,448,239 + BT4150 0.40 -2.0 5,448,239 + BT4150 0.40 -3.1 5,448,240 - BT4150 0.40 -1.5 5,448,357 + BT4150 0.50 +2.1 5,448,471 + BT4150 0.59 -0.3 5,448,471 + BT4150 0.59 +1.1 5,448,496 - BT4150 0.61 -0.2 5,448,660 + BT4150 0.74 -1.0
Or see this region's nucleotide sequence