Strain Fitness in Bacteroides thetaiotaomicron VPI-5482 around BT3972

Experiment: BHIS with Trimethoprim 0.0125 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBT3970 and BT3971 are separated by 391 nucleotidesBT3971 and BT3972 overlap by 23 nucleotidesBT3972 and BT3973 are separated by 20 nucleotides BT3970: BT3970 - hypothetical protein (NCBI ptt file), at 5,171,025 to 5,171,741 BT3970 BT3971: BT3971 - glutathione peroxidase (NCBI ptt file), at 5,172,133 to 5,172,675 BT3971 BT3972: BT3972 - putative NTP pyrophosphohydrolase (NCBI ptt file), at 5,172,653 to 5,173,195 BT3972 BT3973: BT3973 - S-adenosylmethionine:tRNA ribosyltransferase-isomerase (NCBI ptt file), at 5,173,216 to 5,174,436 BT3973 Position (kb) 5172 5173 5174Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1 2 3at 5171.731 kb on - strandat 5171.752 kb on + strandat 5171.764 kb on - strandat 5171.777 kb on - strandat 5171.780 kb on + strandat 5171.780 kb on + strandat 5171.780 kb on + strandat 5171.963 kb on - strandat 5171.963 kb on - strandat 5171.965 kb on - strandat 5171.966 kb on + strandat 5171.971 kb on + strandat 5171.972 kb on - strandat 5171.976 kb on - strandat 5171.990 kb on - strandat 5172.002 kb on + strandat 5172.004 kb on + strandat 5172.005 kb on - strandat 5172.045 kb on + strandat 5172.066 kb on - strandat 5172.102 kb on + strandat 5172.107 kb on + strandat 5172.118 kb on - strandat 5172.126 kb on - strandat 5172.168 kb on - strandat 5172.195 kb on + strand, within BT3971at 5172.206 kb on - strand, within BT3971at 5172.278 kb on + strand, within BT3971at 5172.281 kb on + strand, within BT3971at 5172.282 kb on - strand, within BT3971at 5172.346 kb on + strand, within BT3971at 5172.355 kb on - strand, within BT3971at 5172.444 kb on - strand, within BT3971at 5172.448 kb on + strand, within BT3971at 5172.456 kb on + strand, within BT3971at 5172.582 kb on + strand, within BT3971at 5172.583 kb on - strand, within BT3971at 5172.674 kb on - strandat 5172.698 kb on - strandat 5172.704 kb on + strandat 5172.705 kb on - strandat 5172.714 kb on - strand, within BT3972at 5172.883 kb on - strand, within BT3972at 5172.956 kb on + strand, within BT3972at 5172.957 kb on - strand, within BT3972at 5172.976 kb on + strand, within BT3972at 5172.976 kb on + strand, within BT3972at 5173.045 kb on - strand, within BT3972at 5173.047 kb on - strand, within BT3972at 5173.047 kb on - strand, within BT3972at 5173.047 kb on - strand, within BT3972at 5173.195 kb on + strandat 5173.196 kb on - strandat 5173.196 kb on - strandat 5173.196 kb on - strandat 5173.278 kb on - strandat 5173.284 kb on - strandat 5173.383 kb on - strand, within BT3973at 5173.383 kb on - strand, within BT3973at 5173.400 kb on - strand, within BT3973at 5173.400 kb on - strand, within BT3973at 5173.413 kb on + strand, within BT3973at 5173.457 kb on - strand, within BT3973at 5173.677 kb on + strand, within BT3973at 5173.679 kb on + strandat 5173.960 kb on + strand, within BT3973at 5174.050 kb on + strand, within BT3973

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Per-strain Table

Position Strand Gene LocusTag Fraction BHIS with Trimethoprim 0.0125 mM
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5,171,731 - -4.4
5,171,752 + -0.5
5,171,764 - +0.8
5,171,777 - -0.5
5,171,780 + -1.1
5,171,780 + -1.5
5,171,780 + -1.5
5,171,963 - +2.4
5,171,963 - -1.5
5,171,965 - -3.6
5,171,966 + -0.9
5,171,971 + +3.5
5,171,972 - -1.9
5,171,976 - +0.8
5,171,990 - +0.1
5,172,002 + -1.9
5,172,004 + -2.2
5,172,005 - +3.5
5,172,045 + -0.2
5,172,066 - +2.9
5,172,102 + -1.6
5,172,107 + -1.8
5,172,118 - -2.5
5,172,126 - +2.1
5,172,168 - -1.8
5,172,195 + BT3971 0.11 -1.8
5,172,206 - BT3971 0.13 -0.9
5,172,278 + BT3971 0.27 +3.1
5,172,281 + BT3971 0.27 +1.1
5,172,282 - BT3971 0.27 -2.2
5,172,346 + BT3971 0.39 -0.6
5,172,355 - BT3971 0.41 -0.5
5,172,444 - BT3971 0.57 +2.7
5,172,448 + BT3971 0.58 -2.9
5,172,456 + BT3971 0.59 +0.1
5,172,582 + BT3971 0.83 -3.6
5,172,583 - BT3971 0.83 +0.7
5,172,674 - +1.7
5,172,698 - -1.3
5,172,704 + -2.2
5,172,705 - +0.1
5,172,714 - BT3972 0.11 -2.0
5,172,883 - BT3972 0.42 -1.4
5,172,956 + BT3972 0.56 -2.7
5,172,957 - BT3972 0.56 -3.9
5,172,976 + BT3972 0.59 -0.9
5,172,976 + BT3972 0.59 +0.1
5,173,045 - BT3972 0.72 -2.4
5,173,047 - BT3972 0.73 -5.1
5,173,047 - BT3972 0.73 +0.7
5,173,047 - BT3972 0.73 +0.6
5,173,195 + -1.9
5,173,196 - -1.3
5,173,196 - -1.1
5,173,196 - -0.7
5,173,278 - +1.1
5,173,284 - -1.5
5,173,383 - BT3973 0.14 +0.1
5,173,383 - BT3973 0.14 -3.4
5,173,400 - BT3973 0.15 -0.9
5,173,400 - BT3973 0.15 -0.9
5,173,413 + BT3973 0.16 +1.1
5,173,457 - BT3973 0.20 +0.1
5,173,677 + BT3973 0.38 -2.2
5,173,679 + +0.1
5,173,960 + BT3973 0.61 -2.7
5,174,050 + BT3973 0.68 -1.9

Or see this region's nucleotide sequence