Experiment: BHIS with Trimethoprim 0.0125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT3783 and BT3784 are separated by 58 nucleotides BT3784 and BT3785 overlap by 4 nucleotides BT3785 and BT3786 overlap by 12 nucleotides
BT3783: BT3783 - conserved hypothetical protein (NCBI ptt file), at 4,909,408 to 4,910,355
BT3783
BT3784: BT3784 - putative alpha-1,2-mannosidase (NCBI ptt file), at 4,910,414 to 4,912,696
BT3784
BT3785: BT3785 - hypothetical protein (NCBI ptt file), at 4,912,693 to 4,912,917
BT3785
BT3786: BT3786 - two-component system sensor histidine kinase/response regulator, hybrid ('one-component system') (NCBI ptt file), at 4,912,906 to 4,916,943
BT3786
Position (kb)
4910
4911
4912
4913 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 4909.470 kb on + strand at 4909.470 kb on + strand at 4909.470 kb on + strand at 4909.481 kb on + strand at 4909.489 kb on - strand at 4909.557 kb on + strand, within BT3783 at 4909.734 kb on - strand, within BT3783 at 4910.090 kb on + strand, within BT3783 at 4910.090 kb on + strand, within BT3783 at 4910.091 kb on - strand, within BT3783 at 4910.262 kb on - strand at 4910.331 kb on + strand at 4910.336 kb on - strand at 4910.677 kb on - strand, within BT3784 at 4911.051 kb on - strand, within BT3784 at 4911.051 kb on - strand, within BT3784 at 4911.273 kb on + strand, within BT3784 at 4911.331 kb on - strand, within BT3784 at 4911.345 kb on + strand, within BT3784 at 4911.496 kb on + strand, within BT3784 at 4911.595 kb on - strand, within BT3784 at 4911.659 kb on - strand, within BT3784 at 4911.719 kb on + strand, within BT3784 at 4911.930 kb on - strand, within BT3784 at 4912.161 kb on - strand, within BT3784 at 4912.165 kb on - strand, within BT3784 at 4912.165 kb on - strand, within BT3784 at 4912.290 kb on - strand, within BT3784 at 4912.402 kb on - strand, within BT3784 at 4912.468 kb on + strand at 4912.470 kb on + strand at 4912.587 kb on - strand at 4912.602 kb on + strand at 4913.002 kb on + strand at 4913.002 kb on + strand at 4913.019 kb on + strand at 4913.022 kb on + strand at 4913.161 kb on - strand at 4913.163 kb on + strand at 4913.182 kb on - strand at 4913.200 kb on + strand at 4913.200 kb on + strand at 4913.203 kb on + strand at 4913.206 kb on - strand at 4913.241 kb on + strand at 4913.250 kb on + strand at 4913.305 kb on - strand at 4913.359 kb on - strand, within BT3786 at 4913.427 kb on + strand, within BT3786 at 4913.428 kb on - strand, within BT3786 at 4913.547 kb on + strand, within BT3786 at 4913.555 kb on - strand, within BT3786 at 4913.573 kb on - strand, within BT3786 at 4913.575 kb on + strand, within BT3786 at 4913.576 kb on - strand, within BT3786 at 4913.590 kb on + strand, within BT3786 at 4913.600 kb on - strand, within BT3786
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Trimethoprim 0.0125 mM remove 4,909,470 + -2.8 4,909,470 + +3.1 4,909,470 + +3.2 4,909,481 + -0.2 4,909,489 - -2.5 4,909,557 + BT3783 0.16 +0.9 4,909,734 - BT3783 0.34 -2.9 4,910,090 + BT3783 0.72 -2.5 4,910,090 + BT3783 0.72 -1.3 4,910,091 - BT3783 0.72 +0.8 4,910,262 - -2.1 4,910,331 + -1.3 4,910,336 - -2.1 4,910,677 - BT3784 0.12 +0.4 4,911,051 - BT3784 0.28 -2.2 4,911,051 - BT3784 0.28 -0.9 4,911,273 + BT3784 0.38 -0.6 4,911,331 - BT3784 0.40 -0.5 4,911,345 + BT3784 0.41 -1.3 4,911,496 + BT3784 0.47 -3.7 4,911,595 - BT3784 0.52 -1.5 4,911,659 - BT3784 0.55 -1.0 4,911,719 + BT3784 0.57 +0.1 4,911,930 - BT3784 0.66 +0.1 4,912,161 - BT3784 0.77 -0.9 4,912,165 - BT3784 0.77 +0.1 4,912,165 - BT3784 0.77 -1.2 4,912,290 - BT3784 0.82 -1.5 4,912,402 - BT3784 0.87 -1.9 4,912,468 + +1.8 4,912,470 + -3.2 4,912,587 - +1.1 4,912,602 + -1.5 4,913,002 + -1.9 4,913,002 + +1.1 4,913,019 + -1.8 4,913,022 + -3.4 4,913,161 - -0.9 4,913,163 + -2.7 4,913,182 - -0.7 4,913,200 + +0.1 4,913,200 + -0.8 4,913,203 + -1.5 4,913,206 - -0.6 4,913,241 + -0.3 4,913,250 + -1.5 4,913,305 - +2.2 4,913,359 - BT3786 0.11 -0.9 4,913,427 + BT3786 0.13 +0.1 4,913,428 - BT3786 0.13 -3.1 4,913,547 + BT3786 0.16 +0.1 4,913,555 - BT3786 0.16 +1.9 4,913,573 - BT3786 0.17 -0.9 4,913,575 + BT3786 0.17 -2.5 4,913,576 - BT3786 0.17 -0.1 4,913,590 + BT3786 0.17 -1.8 4,913,600 - BT3786 0.17 +0.1
Or see this region's nucleotide sequence