Experiment: BHIS with Trimethoprim 0.0125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT2133 and BT2134 are separated by 90 nucleotides BT2134 and BT2135 are separated by 75 nucleotides BT2135 and BT2136 overlap by 12 nucleotides
BT2133: BT2133 - conserved hypothetical protein (NCBI ptt file), at 2,684,368 to 2,687,076
BT2133
BT2134: BT2134 - putative HD superfamily hydrolase (NCBI ptt file), at 2,687,167 to 2,687,703
BT2134
BT2135: BT2135 - conserved hypothetical protein (NCBI ptt file), at 2,687,779 to 2,688,942
BT2135
BT2136: BT2136 - putative purple acid phosphatase (NCBI ptt file), at 2,688,931 to 2,690,289
BT2136
Position (kb)
2687
2688
2689 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 2687.278 kb on - strand, within BT2134 at 2687.305 kb on + strand, within BT2134 at 2687.306 kb on - strand, within BT2134 at 2687.306 kb on - strand, within BT2134 at 2687.477 kb on + strand, within BT2134 at 2687.530 kb on + strand, within BT2134 at 2687.531 kb on - strand, within BT2134 at 2687.579 kb on - strand, within BT2134 at 2687.584 kb on - strand, within BT2134 at 2687.596 kb on + strand, within BT2134 at 2687.631 kb on + strand, within BT2134 at 2687.631 kb on + strand, within BT2134 at 2687.632 kb on - strand, within BT2134 at 2687.636 kb on - strand, within BT2134 at 2687.636 kb on - strand, within BT2134 at 2687.638 kb on + strand, within BT2134 at 2687.704 kb on - strand at 2687.718 kb on + strand at 2687.722 kb on - strand at 2687.740 kb on - strand at 2687.779 kb on - strand at 2687.836 kb on + strand at 2687.836 kb on + strand at 2687.836 kb on + strand at 2687.857 kb on - strand at 2687.872 kb on - strand at 2687.881 kb on + strand at 2688.054 kb on - strand, within BT2135 at 2688.149 kb on + strand, within BT2135 at 2688.149 kb on + strand, within BT2135 at 2688.150 kb on - strand, within BT2135 at 2688.189 kb on + strand, within BT2135 at 2688.189 kb on + strand, within BT2135 at 2688.193 kb on + strand, within BT2135 at 2688.194 kb on - strand, within BT2135 at 2688.194 kb on - strand, within BT2135 at 2688.194 kb on - strand, within BT2135 at 2688.194 kb on - strand, within BT2135 at 2688.194 kb on - strand, within BT2135 at 2688.194 kb on - strand, within BT2135 at 2688.195 kb on + strand, within BT2135 at 2688.195 kb on + strand, within BT2135 at 2688.195 kb on + strand, within BT2135 at 2688.201 kb on + strand, within BT2135 at 2688.208 kb on + strand, within BT2135 at 2688.209 kb on - strand, within BT2135 at 2688.252 kb on + strand, within BT2135 at 2688.253 kb on - strand, within BT2135 at 2688.300 kb on - strand, within BT2135 at 2688.358 kb on - strand, within BT2135 at 2688.443 kb on + strand, within BT2135 at 2688.620 kb on - strand, within BT2135 at 2688.695 kb on - strand, within BT2135 at 2688.745 kb on + strand, within BT2135 at 2688.937 kb on + strand at 2689.095 kb on - strand, within BT2136 at 2689.116 kb on - strand, within BT2136 at 2689.161 kb on - strand, within BT2136 at 2689.190 kb on + strand, within BT2136 at 2689.244 kb on + strand, within BT2136 at 2689.279 kb on - strand, within BT2136 at 2689.295 kb on + strand, within BT2136 at 2689.296 kb on - strand, within BT2136 at 2689.296 kb on - strand, within BT2136 at 2689.299 kb on + strand, within BT2136 at 2689.300 kb on - strand, within BT2136 at 2689.300 kb on - strand, within BT2136 at 2689.393 kb on + strand, within BT2136 at 2689.400 kb on + strand, within BT2136 at 2689.400 kb on + strand, within BT2136 at 2689.402 kb on + strand, within BT2136 at 2689.549 kb on + strand, within BT2136 at 2689.550 kb on - strand, within BT2136 at 2689.716 kb on - strand, within BT2136 at 2689.755 kb on + strand, within BT2136 at 2689.758 kb on - strand, within BT2136 at 2689.842 kb on + strand, within BT2136
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Trimethoprim 0.0125 mM remove 2,687,278 - BT2134 0.21 +0.1 2,687,305 + BT2134 0.26 -0.4 2,687,306 - BT2134 0.26 -1.5 2,687,306 - BT2134 0.26 -0.9 2,687,477 + BT2134 0.58 -1.8 2,687,530 + BT2134 0.68 -0.9 2,687,531 - BT2134 0.68 +2.1 2,687,579 - BT2134 0.77 -1.5 2,687,584 - BT2134 0.78 -1.5 2,687,596 + BT2134 0.80 +0.1 2,687,631 + BT2134 0.86 +0.5 2,687,631 + BT2134 0.86 -3.4 2,687,632 - BT2134 0.87 +2.9 2,687,636 - BT2134 0.87 +2.4 2,687,636 - BT2134 0.87 -3.4 2,687,638 + BT2134 0.88 -2.3 2,687,704 - -0.3 2,687,718 + -2.9 2,687,722 - +0.5 2,687,740 - +4.0 2,687,779 - -0.0 2,687,836 + -2.3 2,687,836 + +1.9 2,687,836 + -2.5 2,687,857 - +1.4 2,687,872 - +0.1 2,687,881 + -0.7 2,688,054 - BT2135 0.24 +2.7 2,688,149 + BT2135 0.32 +0.1 2,688,149 + BT2135 0.32 +1.7 2,688,150 - BT2135 0.32 -1.5 2,688,189 + BT2135 0.35 +0.5 2,688,189 + BT2135 0.35 +0.1 2,688,193 + BT2135 0.36 -3.3 2,688,194 - BT2135 0.36 -2.1 2,688,194 - BT2135 0.36 -0.4 2,688,194 - BT2135 0.36 +0.1 2,688,194 - BT2135 0.36 -0.5 2,688,194 - BT2135 0.36 -1.9 2,688,194 - BT2135 0.36 -1.5 2,688,195 + BT2135 0.36 -1.7 2,688,195 + BT2135 0.36 -2.5 2,688,195 + BT2135 0.36 -4.3 2,688,201 + BT2135 0.36 +0.1 2,688,208 + BT2135 0.37 +2.4 2,688,209 - BT2135 0.37 -1.5 2,688,252 + BT2135 0.41 -2.3 2,688,253 - BT2135 0.41 -1.5 2,688,300 - BT2135 0.45 -1.6 2,688,358 - BT2135 0.50 -2.7 2,688,443 + BT2135 0.57 -0.9 2,688,620 - BT2135 0.72 +0.1 2,688,695 - BT2135 0.79 -2.7 2,688,745 + BT2135 0.83 +1.7 2,688,937 + +0.1 2,689,095 - BT2136 0.12 +2.1 2,689,116 - BT2136 0.14 +1.5 2,689,161 - BT2136 0.17 +0.7 2,689,190 + BT2136 0.19 -3.6 2,689,244 + BT2136 0.23 +1.0 2,689,279 - BT2136 0.26 -2.8 2,689,295 + BT2136 0.27 -3.4 2,689,296 - BT2136 0.27 +0.5 2,689,296 - BT2136 0.27 +0.1 2,689,299 + BT2136 0.27 -1.9 2,689,300 - BT2136 0.27 +0.1 2,689,300 - BT2136 0.27 +2.9 2,689,393 + BT2136 0.34 +0.1 2,689,400 + BT2136 0.35 +1.9 2,689,400 + BT2136 0.35 +0.5 2,689,402 + BT2136 0.35 +2.1 2,689,549 + BT2136 0.45 -1.5 2,689,550 - BT2136 0.46 -0.5 2,689,716 - BT2136 0.58 -2.9 2,689,755 + BT2136 0.61 -0.9 2,689,758 - BT2136 0.61 -0.9 2,689,842 + BT2136 0.67 -0.5
Or see this region's nucleotide sequence