Experiment: BHIS with Trimethoprim 0.0125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1843 and BT1844 overlap by 4 nucleotides BT1844 and BT1845 are separated by 160 nucleotides BT1845 and BT1846 are separated by 8 nucleotides
BT1843: BT1843 - adenylosuccinate synthetase (NCBI ptt file), at 2,314,782 to 2,316,053
BT1843
BT1844: BT1844 - transcriptional regulator, putative iron uptake regulation protein (NCBI ptt file), at 2,316,050 to 2,316,541
BT1844
BT1845: BT1845 - conserved hypothetical protein (NCBI ptt file), at 2,316,702 to 2,317,367
BT1845
BT1846: BT1846 - putative dipeptidyl-peptidase III (NCBI ptt file), at 2,317,376 to 2,319,403
BT1846
Position (kb)
2316
2317 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5
6 at 2315.101 kb on - strand, within BT1843 at 2315.101 kb on - strand, within BT1843 at 2315.189 kb on + strand, within BT1843 at 2315.245 kb on - strand, within BT1843 at 2315.302 kb on - strand, within BT1843 at 2315.349 kb on - strand, within BT1843 at 2315.486 kb on + strand, within BT1843 at 2315.524 kb on + strand, within BT1843 at 2315.649 kb on - strand, within BT1843 at 2315.705 kb on - strand, within BT1843 at 2315.901 kb on + strand, within BT1843 at 2315.901 kb on + strand, within BT1843 at 2315.901 kb on + strand, within BT1843 at 2315.905 kb on + strand, within BT1843 at 2315.930 kb on + strand at 2315.930 kb on + strand at 2315.930 kb on + strand at 2315.931 kb on - strand at 2315.931 kb on - strand at 2315.931 kb on - strand at 2315.974 kb on + strand at 2315.985 kb on + strand at 2315.989 kb on - strand at 2316.034 kb on - strand at 2316.046 kb on - strand at 2316.092 kb on - strand at 2316.127 kb on + strand, within BT1844 at 2316.128 kb on - strand, within BT1844 at 2316.130 kb on - strand, within BT1844 at 2316.229 kb on + strand, within BT1844 at 2316.233 kb on + strand, within BT1844 at 2316.233 kb on + strand at 2316.233 kb on + strand, within BT1844 at 2316.274 kb on - strand, within BT1844 at 2316.337 kb on + strand, within BT1844 at 2316.341 kb on - strand, within BT1844 at 2316.341 kb on - strand, within BT1844 at 2316.394 kb on - strand, within BT1844 at 2316.400 kb on + strand, within BT1844 at 2316.429 kb on + strand, within BT1844 at 2316.429 kb on + strand, within BT1844 at 2316.490 kb on - strand, within BT1844 at 2316.514 kb on + strand at 2316.568 kb on + strand at 2316.607 kb on + strand at 2316.612 kb on - strand at 2316.618 kb on + strand at 2316.619 kb on - strand at 2316.625 kb on + strand at 2316.628 kb on + strand at 2316.632 kb on + strand at 2316.633 kb on - strand at 2316.642 kb on - strand at 2316.642 kb on - strand at 2316.645 kb on + strand at 2316.682 kb on + strand at 2316.683 kb on - strand at 2316.683 kb on - strand at 2316.683 kb on - strand at 2316.687 kb on - strand at 2316.693 kb on + strand at 2316.697 kb on - strand at 2316.697 kb on - strand at 2316.700 kb on + strand at 2316.825 kb on - strand, within BT1845 at 2316.825 kb on - strand, within BT1845 at 2316.825 kb on - strand, within BT1845 at 2316.826 kb on + strand, within BT1845 at 2316.827 kb on - strand, within BT1845 at 2317.103 kb on + strand, within BT1845 at 2317.360 kb on - strand at 2317.382 kb on - strand at 2317.405 kb on - strand at 2317.437 kb on + strand at 2317.447 kb on + strand at 2317.501 kb on - strand at 2317.525 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Trimethoprim 0.0125 mM remove 2,315,101 - BT1843 0.25 +3.0 2,315,101 - BT1843 0.25 +2.5 2,315,189 + BT1843 0.32 +2.7 2,315,245 - BT1843 0.36 +1.5 2,315,302 - BT1843 0.41 -1.1 2,315,349 - BT1843 0.45 -2.2 2,315,486 + BT1843 0.55 +1.0 2,315,524 + BT1843 0.58 +0.4 2,315,649 - BT1843 0.68 +0.6 2,315,705 - BT1843 0.73 -0.7 2,315,901 + BT1843 0.88 +1.9 2,315,901 + BT1843 0.88 +2.8 2,315,901 + BT1843 0.88 +1.1 2,315,905 + BT1843 0.88 +1.1 2,315,930 + +1.0 2,315,930 + +0.1 2,315,930 + -3.3 2,315,931 - +0.1 2,315,931 - +0.4 2,315,931 - +3.8 2,315,974 + -1.4 2,315,985 + +2.2 2,315,989 - +1.1 2,316,034 - +0.1 2,316,046 - +1.1 2,316,092 - -3.3 2,316,127 + BT1844 0.16 +1.9 2,316,128 - BT1844 0.16 +1.5 2,316,130 - BT1844 0.16 +3.5 2,316,229 + BT1844 0.36 +1.9 2,316,233 + BT1844 0.37 +5.7 2,316,233 + +2.8 2,316,233 + BT1844 0.37 +3.4 2,316,274 - BT1844 0.46 +3.3 2,316,337 + BT1844 0.58 +3.3 2,316,341 - BT1844 0.59 +1.7 2,316,341 - BT1844 0.59 +2.3 2,316,394 - BT1844 0.70 +5.9 2,316,400 + BT1844 0.71 +2.1 2,316,429 + BT1844 0.77 +3.0 2,316,429 + BT1844 0.77 +4.2 2,316,490 - BT1844 0.89 +5.3 2,316,514 + +5.6 2,316,568 + +2.3 2,316,607 + +0.7 2,316,612 - -1.2 2,316,618 + +0.5 2,316,619 - -1.4 2,316,625 + -1.4 2,316,628 + -0.9 2,316,632 + -1.2 2,316,633 - +2.8 2,316,642 - +2.3 2,316,642 - +1.1 2,316,645 + -0.9 2,316,682 + +0.2 2,316,683 - +0.3 2,316,683 - +4.3 2,316,683 - +1.1 2,316,687 - -0.5 2,316,693 + +0.9 2,316,697 - +0.7 2,316,697 - +2.5 2,316,700 + +1.9 2,316,825 - BT1845 0.18 -0.9 2,316,825 - BT1845 0.18 -0.5 2,316,825 - BT1845 0.18 -2.7 2,316,826 + BT1845 0.19 +0.1 2,316,827 - BT1845 0.19 +0.1 2,317,103 + BT1845 0.60 -2.2 2,317,360 - -0.9 2,317,382 - -0.4 2,317,405 - +1.7 2,317,437 + -1.2 2,317,447 + -1.2 2,317,501 - -3.3 2,317,525 - +1.1
Or see this region's nucleotide sequence