Experiment: BHIS with Trimethoprim 0.0125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1836 and BT1837 are separated by 63 nucleotides BT1837 and BT1838 are separated by 103 nucleotides
BT1836: BT1836 - thiamine biosynthesis protein ThiH (NCBI ptt file), at 2,303,610 to 2,305,028
BT1836
BT1837: BT1837 - putative GTP-binding protein, putative GTPase (NCBI ptt file), at 2,305,092 to 2,306,309
BT1837
BT1838: BT1838 - putative alanyl dipeptidyl peptidase (NCBI ptt file), at 2,306,413 to 2,308,935
BT1838
Position (kb)
2305
2306
2307 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1
2
3 at 2304.092 kb on + strand, within BT1836 at 2304.093 kb on - strand, within BT1836 at 2304.095 kb on + strand, within BT1836 at 2304.133 kb on + strand, within BT1836 at 2304.134 kb on - strand, within BT1836 at 2304.195 kb on + strand, within BT1836 at 2304.196 kb on - strand, within BT1836 at 2304.196 kb on - strand, within BT1836 at 2304.196 kb on - strand, within BT1836 at 2304.196 kb on - strand, within BT1836 at 2304.216 kb on + strand, within BT1836 at 2304.231 kb on + strand, within BT1836 at 2304.232 kb on - strand, within BT1836 at 2304.232 kb on - strand, within BT1836 at 2304.268 kb on - strand, within BT1836 at 2304.274 kb on - strand, within BT1836 at 2304.274 kb on - strand, within BT1836 at 2304.323 kb on - strand, within BT1836 at 2304.323 kb on - strand, within BT1836 at 2304.335 kb on - strand, within BT1836 at 2304.355 kb on - strand, within BT1836 at 2304.500 kb on + strand, within BT1836 at 2304.557 kb on + strand, within BT1836 at 2304.557 kb on + strand, within BT1836 at 2304.807 kb on - strand, within BT1836 at 2304.973 kb on + strand at 2304.974 kb on - strand at 2305.073 kb on + strand at 2305.092 kb on - strand at 2305.290 kb on - strand, within BT1837 at 2305.503 kb on - strand, within BT1837 at 2305.652 kb on + strand, within BT1837 at 2305.652 kb on + strand, within BT1837 at 2305.653 kb on - strand, within BT1837 at 2305.709 kb on + strand, within BT1837 at 2305.733 kb on + strand, within BT1837 at 2305.810 kb on + strand, within BT1837 at 2305.884 kb on + strand, within BT1837 at 2305.943 kb on + strand, within BT1837 at 2306.012 kb on - strand, within BT1837 at 2306.066 kb on - strand, within BT1837 at 2306.114 kb on - strand, within BT1837 at 2306.114 kb on - strand, within BT1837 at 2306.151 kb on - strand, within BT1837 at 2306.257 kb on - strand at 2306.373 kb on - strand at 2306.376 kb on + strand at 2306.386 kb on - strand at 2306.391 kb on + strand at 2306.445 kb on + strand at 2306.449 kb on - strand at 2306.449 kb on - strand at 2306.535 kb on + strand at 2306.585 kb on + strand at 2306.674 kb on + strand, within BT1838 at 2306.993 kb on + strand, within BT1838 at 2307.000 kb on + strand, within BT1838 at 2307.008 kb on - strand, within BT1838 at 2307.052 kb on + strand, within BT1838 at 2307.052 kb on + strand, within BT1838 at 2307.103 kb on + strand, within BT1838 at 2307.122 kb on + strand, within BT1838 at 2307.138 kb on - strand, within BT1838 at 2307.187 kb on - strand, within BT1838 at 2307.191 kb on + strand, within BT1838 at 2307.191 kb on + strand, within BT1838 at 2307.192 kb on - strand, within BT1838 at 2307.196 kb on + strand, within BT1838 at 2307.196 kb on + strand, within BT1838 at 2307.196 kb on + strand, within BT1838
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Trimethoprim 0.0125 mM remove 2,304,092 + BT1836 0.34 -2.2 2,304,093 - BT1836 0.34 +0.5 2,304,095 + BT1836 0.34 -2.7 2,304,133 + BT1836 0.37 -2.5 2,304,134 - BT1836 0.37 -1.5 2,304,195 + BT1836 0.41 -2.5 2,304,196 - BT1836 0.41 -1.2 2,304,196 - BT1836 0.41 -4.2 2,304,196 - BT1836 0.41 -1.9 2,304,196 - BT1836 0.41 -2.8 2,304,216 + BT1836 0.43 -3.7 2,304,231 + BT1836 0.44 -1.9 2,304,232 - BT1836 0.44 +0.1 2,304,232 - BT1836 0.44 -2.7 2,304,268 - BT1836 0.46 -0.7 2,304,274 - BT1836 0.47 -3.8 2,304,274 - BT1836 0.47 -1.5 2,304,323 - BT1836 0.50 -4.8 2,304,323 - BT1836 0.50 +0.1 2,304,335 - BT1836 0.51 +0.1 2,304,355 - BT1836 0.53 -3.9 2,304,500 + BT1836 0.63 -1.9 2,304,557 + BT1836 0.67 -1.5 2,304,557 + BT1836 0.67 -2.5 2,304,807 - BT1836 0.84 -1.9 2,304,973 + -0.2 2,304,974 - +0.5 2,305,073 + +2.5 2,305,092 - +0.6 2,305,290 - BT1837 0.16 +1.7 2,305,503 - BT1837 0.34 -5.8 2,305,652 + BT1837 0.46 -3.7 2,305,652 + BT1837 0.46 -0.9 2,305,653 - BT1837 0.46 -0.4 2,305,709 + BT1837 0.51 -3.2 2,305,733 + BT1837 0.53 -1.9 2,305,810 + BT1837 0.59 +0.7 2,305,884 + BT1837 0.65 -0.9 2,305,943 + BT1837 0.70 -4.5 2,306,012 - BT1837 0.76 -3.2 2,306,066 - BT1837 0.80 +1.7 2,306,114 - BT1837 0.84 -2.2 2,306,114 - BT1837 0.84 -3.0 2,306,151 - BT1837 0.87 -0.9 2,306,257 - -1.9 2,306,373 - -0.4 2,306,376 + -3.0 2,306,386 - -0.9 2,306,391 + +0.9 2,306,445 + -0.9 2,306,449 - +1.1 2,306,449 - +0.5 2,306,535 + -0.4 2,306,585 + +3.5 2,306,674 + BT1838 0.10 -3.3 2,306,993 + BT1838 0.23 -2.9 2,307,000 + BT1838 0.23 -1.9 2,307,008 - BT1838 0.24 -1.4 2,307,052 + BT1838 0.25 +2.0 2,307,052 + BT1838 0.25 +2.9 2,307,103 + BT1838 0.27 +2.1 2,307,122 + BT1838 0.28 +2.1 2,307,138 - BT1838 0.29 -1.5 2,307,187 - BT1838 0.31 -1.6 2,307,191 + BT1838 0.31 +0.7 2,307,191 + BT1838 0.31 -2.7 2,307,192 - BT1838 0.31 +1.9 2,307,196 + BT1838 0.31 +0.1 2,307,196 + BT1838 0.31 -1.9 2,307,196 + BT1838 0.31 +0.6
Or see this region's nucleotide sequence