Experiment: BHIS with Trimethoprim 0.0125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1782 and BT1783 overlap by 23 nucleotides BT1783 and BT1784 are separated by 186 nucleotides BT1784 and BT1785 are separated by 83 nucleotides
BT1782: BT1782 - putative phosphoesterase (NCBI ptt file), at 2,227,954 to 2,228,799
BT1782
BT1783: BT1783 - cation/H+ antiporter (NCBI ptt file), at 2,228,777 to 2,231,002
BT1783
BT1784: BT1784 - hypothetical protein (NCBI ptt file), at 2,231,189 to 2,231,710
BT1784
BT1785: BT1785 - hypothetical protein (NCBI ptt file), at 2,231,794 to 2,232,999
BT1785
Position (kb)
2228
2229
2230
2231
2232 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 2227.908 kb on + strand at 2227.917 kb on + strand at 2227.947 kb on + strand at 2227.948 kb on - strand at 2228.018 kb on - strand at 2228.187 kb on + strand, within BT1782 at 2228.188 kb on - strand, within BT1782 at 2228.230 kb on - strand, within BT1782 at 2228.233 kb on + strand, within BT1782 at 2228.234 kb on - strand, within BT1782 at 2228.253 kb on - strand, within BT1782 at 2228.265 kb on + strand, within BT1782 at 2228.266 kb on - strand, within BT1782 at 2228.284 kb on - strand, within BT1782 at 2228.290 kb on + strand, within BT1782 at 2228.290 kb on + strand, within BT1782 at 2228.291 kb on - strand, within BT1782 at 2228.291 kb on - strand, within BT1782 at 2228.291 kb on - strand, within BT1782 at 2228.373 kb on + strand, within BT1782 at 2228.412 kb on - strand, within BT1782 at 2228.479 kb on - strand, within BT1782 at 2228.484 kb on - strand, within BT1782 at 2228.489 kb on + strand, within BT1782 at 2228.489 kb on + strand, within BT1782 at 2228.525 kb on - strand, within BT1782 at 2228.544 kb on + strand, within BT1782 at 2228.545 kb on - strand, within BT1782 at 2228.575 kb on - strand, within BT1782 at 2228.576 kb on + strand, within BT1782 at 2228.577 kb on - strand, within BT1782 at 2228.577 kb on - strand, within BT1782 at 2228.583 kb on - strand, within BT1782 at 2228.605 kb on - strand, within BT1782 at 2228.639 kb on + strand, within BT1782 at 2228.682 kb on - strand, within BT1782 at 2228.695 kb on - strand, within BT1782 at 2228.707 kb on + strand, within BT1782 at 2228.712 kb on - strand, within BT1782 at 2228.735 kb on - strand at 2228.762 kb on - strand at 2228.762 kb on - strand at 2228.764 kb on + strand at 2228.765 kb on - strand at 2228.812 kb on + strand at 2228.885 kb on - strand at 2228.886 kb on + strand at 2228.967 kb on - strand at 2228.980 kb on - strand at 2229.072 kb on - strand, within BT1783 at 2229.073 kb on + strand, within BT1783 at 2229.073 kb on + strand, within BT1783 at 2229.090 kb on - strand, within BT1783 at 2229.096 kb on - strand, within BT1783 at 2229.148 kb on + strand, within BT1783 at 2229.149 kb on - strand, within BT1783 at 2229.227 kb on + strand, within BT1783 at 2229.232 kb on + strand, within BT1783 at 2229.233 kb on - strand, within BT1783 at 2229.233 kb on - strand, within BT1783 at 2229.333 kb on - strand, within BT1783 at 2229.463 kb on + strand, within BT1783 at 2229.463 kb on + strand, within BT1783 at 2229.464 kb on - strand, within BT1783 at 2229.464 kb on - strand, within BT1783 at 2229.464 kb on - strand, within BT1783 at 2229.465 kb on + strand, within BT1783 at 2229.465 kb on + strand, within BT1783 at 2229.466 kb on - strand, within BT1783 at 2229.646 kb on + strand, within BT1783 at 2229.668 kb on - strand, within BT1783 at 2229.782 kb on - strand, within BT1783 at 2229.890 kb on - strand, within BT1783 at 2229.902 kb on + strand, within BT1783 at 2229.902 kb on + strand, within BT1783 at 2229.903 kb on - strand, within BT1783 at 2229.935 kb on + strand, within BT1783 at 2229.935 kb on + strand, within BT1783 at 2230.141 kb on - strand, within BT1783 at 2230.202 kb on + strand, within BT1783 at 2230.256 kb on - strand, within BT1783 at 2230.391 kb on - strand, within BT1783 at 2230.409 kb on - strand, within BT1783 at 2230.435 kb on + strand, within BT1783 at 2230.449 kb on + strand, within BT1783 at 2230.449 kb on + strand, within BT1783 at 2230.536 kb on - strand, within BT1783 at 2230.536 kb on - strand, within BT1783 at 2230.703 kb on - strand, within BT1783 at 2230.790 kb on - strand at 2230.798 kb on + strand at 2230.798 kb on + strand at 2230.799 kb on - strand at 2230.802 kb on + strand at 2230.803 kb on - strand at 2230.838 kb on - strand at 2230.871 kb on + strand at 2230.924 kb on + strand at 2230.964 kb on + strand at 2230.972 kb on + strand at 2230.994 kb on - strand at 2231.003 kb on - strand at 2231.035 kb on - strand at 2231.137 kb on + strand at 2231.138 kb on - strand at 2231.197 kb on - strand at 2231.388 kb on - strand, within BT1784 at 2231.393 kb on + strand, within BT1784 at 2231.398 kb on + strand, within BT1784 at 2231.399 kb on - strand, within BT1784 at 2231.399 kb on - strand, within BT1784 at 2231.400 kb on + strand, within BT1784 at 2231.545 kb on + strand, within BT1784 at 2231.554 kb on - strand, within BT1784 at 2231.647 kb on + strand, within BT1784 at 2231.648 kb on - strand, within BT1784 at 2231.669 kb on - strand at 2231.669 kb on - strand at 2231.682 kb on - strand at 2231.686 kb on - strand at 2231.704 kb on + strand at 2231.811 kb on + strand at 2231.813 kb on + strand at 2231.814 kb on - strand at 2231.898 kb on + strand at 2231.898 kb on + strand at 2231.998 kb on - strand, within BT1785 at 2232.000 kb on - strand, within BT1785
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Trimethoprim 0.0125 mM remove 2,227,908 + +0.1 2,227,917 + +0.8 2,227,947 + +2.1 2,227,948 - +0.1 2,228,018 - -1.5 2,228,187 + BT1782 0.28 +3.9 2,228,188 - BT1782 0.28 -2.1 2,228,230 - BT1782 0.33 +2.1 2,228,233 + BT1782 0.33 -0.7 2,228,234 - BT1782 0.33 -1.5 2,228,253 - BT1782 0.35 +1.7 2,228,265 + BT1782 0.37 -1.9 2,228,266 - BT1782 0.37 +0.3 2,228,284 - BT1782 0.39 -1.9 2,228,290 + BT1782 0.40 +2.9 2,228,290 + BT1782 0.40 +2.5 2,228,291 - BT1782 0.40 +3.0 2,228,291 - BT1782 0.40 -1.7 2,228,291 - BT1782 0.40 +1.4 2,228,373 + BT1782 0.50 -1.9 2,228,412 - BT1782 0.54 +1.1 2,228,479 - BT1782 0.62 +2.5 2,228,484 - BT1782 0.63 +0.1 2,228,489 + BT1782 0.63 +0.1 2,228,489 + BT1782 0.63 -0.3 2,228,525 - BT1782 0.67 -0.7 2,228,544 + BT1782 0.70 +2.1 2,228,545 - BT1782 0.70 -0.5 2,228,575 - BT1782 0.73 -1.9 2,228,576 + BT1782 0.74 -1.8 2,228,577 - BT1782 0.74 +0.1 2,228,577 - BT1782 0.74 +1.9 2,228,583 - BT1782 0.74 -1.9 2,228,605 - BT1782 0.77 +2.1 2,228,639 + BT1782 0.81 +0.1 2,228,682 - BT1782 0.86 -1.2 2,228,695 - BT1782 0.88 -0.3 2,228,707 + BT1782 0.89 +2.3 2,228,712 - BT1782 0.90 +1.7 2,228,735 - -0.8 2,228,762 - -0.6 2,228,762 - +0.7 2,228,764 + -0.4 2,228,765 - +2.7 2,228,812 + +3.1 2,228,885 - -0.8 2,228,886 + -0.9 2,228,967 - -2.5 2,228,980 - +0.9 2,229,072 - BT1783 0.13 +2.4 2,229,073 + BT1783 0.13 -1.9 2,229,073 + BT1783 0.13 -0.1 2,229,090 - BT1783 0.14 +0.6 2,229,096 - BT1783 0.14 +1.1 2,229,148 + BT1783 0.17 -1.5 2,229,149 - BT1783 0.17 +1.7 2,229,227 + BT1783 0.20 -1.8 2,229,232 + BT1783 0.20 -0.9 2,229,233 - BT1783 0.20 -0.6 2,229,233 - BT1783 0.20 -1.9 2,229,333 - BT1783 0.25 +2.3 2,229,463 + BT1783 0.31 +0.5 2,229,463 + BT1783 0.31 +1.1 2,229,464 - BT1783 0.31 -0.5 2,229,464 - BT1783 0.31 +1.4 2,229,464 - BT1783 0.31 -1.6 2,229,465 + BT1783 0.31 +1.7 2,229,465 + BT1783 0.31 -3.1 2,229,466 - BT1783 0.31 -1.9 2,229,646 + BT1783 0.39 -0.8 2,229,668 - BT1783 0.40 +2.7 2,229,782 - BT1783 0.45 +1.4 2,229,890 - BT1783 0.50 +1.8 2,229,902 + BT1783 0.51 -1.7 2,229,902 + BT1783 0.51 -0.9 2,229,903 - BT1783 0.51 +4.2 2,229,935 + BT1783 0.52 +2.4 2,229,935 + BT1783 0.52 +2.3 2,230,141 - BT1783 0.61 +2.8 2,230,202 + BT1783 0.64 +1.5 2,230,256 - BT1783 0.66 -3.2 2,230,391 - BT1783 0.73 -1.5 2,230,409 - BT1783 0.73 +2.2 2,230,435 + BT1783 0.74 -2.5 2,230,449 + BT1783 0.75 +1.4 2,230,449 + BT1783 0.75 -2.3 2,230,536 - BT1783 0.79 +1.1 2,230,536 - BT1783 0.79 -2.7 2,230,703 - BT1783 0.87 +2.9 2,230,790 - -0.3 2,230,798 + +0.1 2,230,798 + +3.2 2,230,799 - -1.2 2,230,802 + +1.7 2,230,803 - +1.4 2,230,838 - -0.5 2,230,871 + -1.7 2,230,924 + -0.7 2,230,964 + -0.3 2,230,972 + -1.8 2,230,994 - +1.7 2,231,003 - +1.9 2,231,035 - +2.1 2,231,137 + -3.1 2,231,138 - -0.9 2,231,197 - +0.4 2,231,388 - BT1784 0.38 -2.7 2,231,393 + BT1784 0.39 +1.1 2,231,398 + BT1784 0.40 -0.9 2,231,399 - BT1784 0.40 -3.2 2,231,399 - BT1784 0.40 -0.8 2,231,400 + BT1784 0.40 -1.1 2,231,545 + BT1784 0.68 -0.9 2,231,554 - BT1784 0.70 -1.5 2,231,647 + BT1784 0.88 -1.4 2,231,648 - BT1784 0.88 -0.9 2,231,669 - -2.9 2,231,669 - +0.8 2,231,682 - +1.9 2,231,686 - -0.9 2,231,704 + -0.5 2,231,811 + -3.2 2,231,813 + -0.5 2,231,814 - +0.1 2,231,898 + -1.8 2,231,898 + +2.4 2,231,998 - BT1785 0.17 +1.4 2,232,000 - BT1785 0.17 -0.5
Or see this region's nucleotide sequence