Experiment: BHIS with Trimethoprim 0.0125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1711 and BT1712 overlap by 4 nucleotides BT1712 and BT1713 are separated by 12 nucleotides BT1713 and BT1714 are separated by 92 nucleotides
BT1711: BT1711 - hypothetical protein (NCBI ptt file), at 2,107,671 to 2,108,696
BT1711
BT1712: BT1712 - glycosyltransferase (NCBI ptt file), at 2,108,693 to 2,109,871
BT1712
BT1713: BT1713 - putative acylneuraminate cytidylyltransferase (NCBI ptt file), at 2,109,884 to 2,110,378
BT1713
BT1714: BT1714 - sialic acid synthase (N-acetylneuraminate synthase) (NCBI ptt file), at 2,110,471 to 2,111,511
BT1714
Position (kb)
2108
2109
2110 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 2107.742 kb on + strand at 2107.742 kb on + strand at 2107.775 kb on + strand, within BT1711 at 2107.775 kb on + strand, within BT1711 at 2107.967 kb on + strand, within BT1711 at 2107.967 kb on + strand, within BT1711 at 2108.047 kb on + strand, within BT1711 at 2108.093 kb on + strand, within BT1711 at 2108.162 kb on + strand, within BT1711 at 2108.255 kb on + strand, within BT1711 at 2108.259 kb on + strand, within BT1711 at 2108.354 kb on + strand, within BT1711 at 2108.402 kb on + strand, within BT1711 at 2108.417 kb on + strand, within BT1711 at 2108.420 kb on - strand, within BT1711 at 2108.421 kb on + strand, within BT1711 at 2108.451 kb on + strand, within BT1711 at 2108.452 kb on - strand, within BT1711 at 2108.452 kb on - strand, within BT1711 at 2108.454 kb on - strand, within BT1711 at 2108.496 kb on - strand, within BT1711 at 2108.541 kb on - strand, within BT1711 at 2108.553 kb on + strand, within BT1711 at 2108.564 kb on + strand, within BT1711 at 2108.565 kb on - strand, within BT1711 at 2108.576 kb on + strand, within BT1711 at 2108.612 kb on + strand at 2108.642 kb on + strand at 2108.654 kb on + strand at 2108.654 kb on + strand at 2108.655 kb on - strand at 2108.657 kb on - strand at 2108.659 kb on + strand at 2108.669 kb on + strand at 2108.672 kb on + strand at 2108.673 kb on - strand at 2108.690 kb on - strand at 2108.698 kb on + strand at 2108.806 kb on + strand at 2108.823 kb on + strand, within BT1712 at 2108.824 kb on - strand, within BT1712 at 2108.825 kb on + strand, within BT1712 at 2108.825 kb on + strand, within BT1712 at 2108.825 kb on + strand, within BT1712 at 2108.825 kb on + strand, within BT1712 at 2108.827 kb on + strand, within BT1712 at 2108.896 kb on + strand, within BT1712 at 2108.911 kb on + strand, within BT1712 at 2108.972 kb on + strand, within BT1712 at 2109.038 kb on - strand, within BT1712 at 2109.101 kb on + strand, within BT1712 at 2109.239 kb on + strand, within BT1712 at 2109.239 kb on + strand, within BT1712 at 2109.268 kb on + strand, within BT1712 at 2109.298 kb on + strand, within BT1712 at 2109.392 kb on + strand, within BT1712 at 2109.400 kb on + strand, within BT1712 at 2109.400 kb on + strand, within BT1712 at 2109.432 kb on + strand, within BT1712 at 2109.439 kb on + strand, within BT1712 at 2109.439 kb on + strand, within BT1712 at 2109.448 kb on + strand, within BT1712 at 2109.449 kb on - strand, within BT1712 at 2109.496 kb on + strand, within BT1712 at 2109.539 kb on + strand, within BT1712 at 2109.539 kb on + strand, within BT1712 at 2109.612 kb on + strand, within BT1712 at 2109.614 kb on + strand, within BT1712 at 2109.614 kb on + strand, within BT1712 at 2109.625 kb on + strand, within BT1712 at 2109.680 kb on + strand, within BT1712 at 2109.747 kb on + strand, within BT1712 at 2109.856 kb on + strand at 2109.982 kb on + strand, within BT1713 at 2109.985 kb on + strand, within BT1713 at 2109.994 kb on + strand, within BT1713 at 2109.994 kb on + strand, within BT1713 at 2110.033 kb on + strand, within BT1713 at 2110.037 kb on + strand, within BT1713 at 2110.118 kb on + strand, within BT1713 at 2110.142 kb on - strand, within BT1713 at 2110.200 kb on - strand, within BT1713 at 2110.243 kb on + strand, within BT1713 at 2110.243 kb on + strand, within BT1713 at 2110.246 kb on + strand, within BT1713 at 2110.315 kb on - strand, within BT1713 at 2110.315 kb on - strand, within BT1713 at 2110.349 kb on - strand at 2110.412 kb on + strand at 2110.433 kb on + strand at 2110.433 kb on + strand at 2110.472 kb on + strand at 2110.562 kb on + strand at 2110.701 kb on + strand, within BT1714 at 2110.720 kb on + strand, within BT1714 at 2110.851 kb on + strand, within BT1714 at 2110.851 kb on + strand, within BT1714 at 2110.867 kb on + strand, within BT1714
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Trimethoprim 0.0125 mM remove 2,107,742 + -2.0 2,107,742 + +3.0 2,107,775 + BT1711 0.10 +1.0 2,107,775 + BT1711 0.10 -1.4 2,107,967 + BT1711 0.29 -1.0 2,107,967 + BT1711 0.29 -2.0 2,108,047 + BT1711 0.37 +0.0 2,108,093 + BT1711 0.41 +2.6 2,108,162 + BT1711 0.48 +0.0 2,108,255 + BT1711 0.57 -0.8 2,108,259 + BT1711 0.57 +0.6 2,108,354 + BT1711 0.67 -2.6 2,108,402 + BT1711 0.71 +1.0 2,108,417 + BT1711 0.73 +1.9 2,108,420 - BT1711 0.73 -1.5 2,108,421 + BT1711 0.73 +2.2 2,108,451 + BT1711 0.76 -2.3 2,108,452 - BT1711 0.76 +3.2 2,108,452 - BT1711 0.76 -2.3 2,108,454 - BT1711 0.76 -0.5 2,108,496 - BT1711 0.80 -1.0 2,108,541 - BT1711 0.85 -2.0 2,108,553 + BT1711 0.86 -1.0 2,108,564 + BT1711 0.87 -0.4 2,108,565 - BT1711 0.87 -0.2 2,108,576 + BT1711 0.88 +1.1 2,108,612 + +1.8 2,108,642 + +0.6 2,108,654 + +1.0 2,108,654 + -1.0 2,108,655 - -1.0 2,108,657 - -3.0 2,108,659 + -2.0 2,108,669 + +1.6 2,108,672 + -1.0 2,108,673 - -2.9 2,108,690 - -2.5 2,108,698 + -2.0 2,108,806 + -1.0 2,108,823 + BT1712 0.11 +0.0 2,108,824 - BT1712 0.11 +1.6 2,108,825 + BT1712 0.11 +2.4 2,108,825 + BT1712 0.11 -1.0 2,108,825 + BT1712 0.11 +0.7 2,108,825 + BT1712 0.11 -1.3 2,108,827 + BT1712 0.11 +0.6 2,108,896 + BT1712 0.17 +3.4 2,108,911 + BT1712 0.18 +0.0 2,108,972 + BT1712 0.24 -0.9 2,109,038 - BT1712 0.29 +2.4 2,109,101 + BT1712 0.35 -2.1 2,109,239 + BT1712 0.46 +1.6 2,109,239 + BT1712 0.46 -0.7 2,109,268 + BT1712 0.49 +0.6 2,109,298 + BT1712 0.51 +0.6 2,109,392 + BT1712 0.59 +0.0 2,109,400 + BT1712 0.60 +0.3 2,109,400 + BT1712 0.60 +2.9 2,109,432 + BT1712 0.63 +0.4 2,109,439 + BT1712 0.63 +1.0 2,109,439 + BT1712 0.63 +0.5 2,109,448 + BT1712 0.64 -0.3 2,109,449 - BT1712 0.64 +1.5 2,109,496 + BT1712 0.68 +0.6 2,109,539 + BT1712 0.72 -2.3 2,109,539 + BT1712 0.72 -1.8 2,109,612 + BT1712 0.78 +2.2 2,109,614 + BT1712 0.78 +2.0 2,109,614 + BT1712 0.78 +0.0 2,109,625 + BT1712 0.79 -1.8 2,109,680 + BT1712 0.84 -0.7 2,109,747 + BT1712 0.89 -1.5 2,109,856 + +0.0 2,109,982 + BT1713 0.20 +4.0 2,109,985 + BT1713 0.20 +2.4 2,109,994 + BT1713 0.22 +0.0 2,109,994 + BT1713 0.22 +0.0 2,110,033 + BT1713 0.30 -1.0 2,110,037 + BT1713 0.31 +2.0 2,110,118 + BT1713 0.47 +0.5 2,110,142 - BT1713 0.52 +0.0 2,110,200 - BT1713 0.64 -2.8 2,110,243 + BT1713 0.73 +0.0 2,110,243 + BT1713 0.73 -1.8 2,110,246 + BT1713 0.73 +2.9 2,110,315 - BT1713 0.87 -2.0 2,110,315 - BT1713 0.87 -2.0 2,110,349 - -3.0 2,110,412 + -3.0 2,110,433 + -0.8 2,110,433 + +2.7 2,110,472 + +2.6 2,110,562 + +2.0 2,110,701 + BT1714 0.22 -1.5 2,110,720 + BT1714 0.24 +0.0 2,110,851 + BT1714 0.37 -1.8 2,110,851 + BT1714 0.37 +3.6 2,110,867 + BT1714 0.38 -2.8
Or see this region's nucleotide sequence