Experiment: BHIS with Trimethoprim 0.0125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1453 and BT1454 are separated by 290 nucleotides BT1454 and BT1455 are separated by 4 nucleotides BT1455 and BT1456 are separated by 512 nucleotides
BT1453: BT1453 - L-lactate permease (NCBI ptt file), at 1,789,860 to 1,791,359
BT1453
BT1454: BT1454 - putative peptide chain release factor (NCBI ptt file), at 1,791,650 to 1,792,270
BT1454
BT1455: BT1455 - protein rtcB (NCBI ptt file), at 1,792,275 to 1,793,672
BT1455
BT1456: BT1456 - thioredoxin (TRX) (NCBI ptt file), at 1,794,185 to 1,794,658
BT1456
Position (kb)
1792
1793
1794 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1791.318 kb on + strand at 1791.318 kb on + strand at 1791.330 kb on - strand at 1791.373 kb on + strand at 1791.377 kb on - strand at 1791.404 kb on + strand at 1791.404 kb on - strand at 1791.405 kb on - strand at 1791.406 kb on + strand at 1791.415 kb on + strand at 1791.415 kb on + strand at 1791.424 kb on + strand at 1791.424 kb on + strand at 1791.425 kb on - strand at 1791.429 kb on - strand at 1791.429 kb on - strand at 1791.450 kb on + strand at 1791.528 kb on + strand at 1791.540 kb on - strand at 1791.551 kb on + strand at 1791.552 kb on - strand at 1791.604 kb on + strand at 1791.629 kb on + strand at 1791.703 kb on + strand at 1791.710 kb on - strand at 1792.245 kb on - strand at 1792.307 kb on + strand at 1792.345 kb on + strand at 1792.345 kb on + strand at 1792.346 kb on - strand at 1792.368 kb on - strand at 1792.631 kb on + strand, within BT1455 at 1792.645 kb on - strand, within BT1455 at 1792.712 kb on + strand, within BT1455 at 1792.713 kb on - strand, within BT1455 at 1792.934 kb on - strand at 1793.141 kb on + strand, within BT1455 at 1793.208 kb on + strand, within BT1455 at 1793.257 kb on - strand, within BT1455 at 1793.267 kb on + strand, within BT1455 at 1793.399 kb on + strand, within BT1455 at 1793.448 kb on + strand, within BT1455 at 1793.495 kb on + strand, within BT1455 at 1793.605 kb on - strand at 1793.622 kb on + strand at 1793.805 kb on + strand at 1793.826 kb on - strand at 1793.826 kb on - strand at 1793.833 kb on + strand at 1793.836 kb on + strand at 1793.931 kb on + strand at 1793.934 kb on - strand at 1793.944 kb on + strand at 1793.949 kb on - strand at 1793.949 kb on - strand at 1793.980 kb on - strand at 1793.980 kb on - strand at 1794.288 kb on + strand, within BT1456 at 1794.289 kb on - strand, within BT1456 at 1794.302 kb on - strand, within BT1456 at 1794.455 kb on + strand, within BT1456 at 1794.456 kb on - strand, within BT1456 at 1794.652 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Trimethoprim 0.0125 mM remove 1,791,318 + -1.7 1,791,318 + +2.4 1,791,330 - -1.2 1,791,373 + -3.0 1,791,377 - +1.7 1,791,404 + -0.7 1,791,404 - -2.7 1,791,405 - +2.6 1,791,406 + +2.7 1,791,415 + +0.9 1,791,415 + -0.6 1,791,424 + -1.1 1,791,424 + -0.1 1,791,425 - -2.1 1,791,429 - +3.3 1,791,429 - +0.1 1,791,450 + -0.5 1,791,528 + +1.0 1,791,540 - +0.4 1,791,551 + +1.9 1,791,552 - +2.3 1,791,604 + -2.6 1,791,629 + -0.3 1,791,703 + -0.1 1,791,710 - +0.9 1,792,245 - +1.0 1,792,307 + +2.7 1,792,345 + -2.1 1,792,345 + -0.7 1,792,346 - -0.5 1,792,368 - -1.1 1,792,631 + BT1455 0.25 -3.1 1,792,645 - BT1455 0.26 -1.1 1,792,712 + BT1455 0.31 -0.1 1,792,713 - BT1455 0.31 -2.7 1,792,934 - -1.1 1,793,141 + BT1455 0.62 -3.7 1,793,208 + BT1455 0.67 +2.2 1,793,257 - BT1455 0.70 -3.0 1,793,267 + BT1455 0.71 -1.7 1,793,399 + BT1455 0.80 -1.1 1,793,448 + BT1455 0.84 -3.0 1,793,495 + BT1455 0.87 +0.9 1,793,605 - -1.5 1,793,622 + -3.0 1,793,805 + +3.2 1,793,826 - -1.1 1,793,826 - -0.7 1,793,833 + -1.4 1,793,836 + -0.1 1,793,931 + -1.6 1,793,934 - -1.7 1,793,944 + +0.4 1,793,949 - -2.5 1,793,949 - -0.1 1,793,980 - -0.1 1,793,980 - -1.7 1,794,288 + BT1456 0.22 -3.1 1,794,289 - BT1456 0.22 -2.5 1,794,302 - BT1456 0.25 +2.4 1,794,455 + BT1456 0.57 -1.2 1,794,456 - BT1456 0.57 -0.7 1,794,652 + -1.1
Or see this region's nucleotide sequence