Experiment: BHIS with Trimethoprim 0.0125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1201 and BT1202 are separated by 82 nucleotides BT1202 and BT1203 overlap by 22 nucleotides BT1203 and BT1204 are separated by 415 nucleotides
BT1201: BT1201 - conserved hypothetical protein (NCBI ptt file), at 1,496,933 to 1,497,562
BT1201
BT1202: BT1202 - UDP-N-acetylglucosamine 2-epimerase (NCBI ptt file), at 1,497,645 to 1,498,121
BT1202
BT1203: BT1203 - putative UDP-N-acetylglucosamine 2-epimerase (NCBI ptt file), at 1,498,100 to 1,498,729
BT1203
BT1204: BT1204 - conserved hypothetical protein, putative outer membrane protein (NCBI ptt file), at 1,499,145 to 1,501,721
BT1204
Position (kb)
1498
1499 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1497.190 kb on + strand, within BT1201 at 1497.193 kb on + strand, within BT1201 at 1497.302 kb on - strand, within BT1201 at 1497.452 kb on + strand, within BT1201 at 1497.565 kb on - strand at 1497.572 kb on - strand at 1497.973 kb on + strand, within BT1202 at 1498.064 kb on + strand, within BT1202 at 1498.151 kb on + strand at 1498.155 kb on - strand at 1498.216 kb on - strand, within BT1203 at 1498.353 kb on + strand, within BT1203 at 1498.370 kb on + strand, within BT1203 at 1498.371 kb on - strand, within BT1203 at 1498.401 kb on + strand, within BT1203 at 1498.514 kb on - strand, within BT1203 at 1498.986 kb on - strand at 1499.047 kb on - strand at 1499.049 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Trimethoprim 0.0125 mM remove 1,497,190 + BT1201 0.41 +2.2 1,497,193 + BT1201 0.41 +1.6 1,497,302 - BT1201 0.59 +3.3 1,497,452 + BT1201 0.82 +1.9 1,497,565 - -0.3 1,497,572 - -0.3 1,497,973 + BT1202 0.69 +0.3 1,498,064 + BT1202 0.88 -0.3 1,498,151 + -0.3 1,498,155 - -0.3 1,498,216 - BT1203 0.18 +1.9 1,498,353 + BT1203 0.40 +2.0 1,498,370 + BT1203 0.43 +0.9 1,498,371 - BT1203 0.43 +2.0 1,498,401 + BT1203 0.48 +1.5 1,498,514 - BT1203 0.66 -0.3 1,498,986 - +0.6 1,499,047 - -1.3 1,499,049 - -3.5
Or see this region's nucleotide sequence