Experiment: BHIS with Trimethoprim 0.0125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1107 and BT1108 are separated by 50 nucleotides BT1108 and BT1109 are separated by 24 nucleotides BT1109 and BT1110 are separated by 161 nucleotides BT1110 and BT1111 are separated by 177 nucleotides
BT1107: BT1107 - ferritin A (NCBI ptt file), at 1,395,440 to 1,395,922
BT1107
BT1108: BT1108 - conserved hypothetical protein, putative integral membrane protein (NCBI ptt file), at 1,395,973 to 1,396,698
BT1108
BT1109: BT1109 - ferritin A (NCBI ptt file), at 1,396,723 to 1,397,175
BT1109
BT1110: BT1110 - hypothetical protein (NCBI ptt file), at 1,397,337 to 1,397,546
BT1110
BT1111: BT1111 - hypothetical protein (NCBI ptt file), at 1,397,724 to 1,397,945
BT1111
Position (kb)
1396
1397
1398 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 1395.762 kb on + strand, within BT1107 at 1395.762 kb on + strand, within BT1107 at 1395.763 kb on - strand, within BT1107 at 1395.851 kb on - strand, within BT1107 at 1395.938 kb on + strand at 1396.285 kb on + strand, within BT1108 at 1396.353 kb on + strand, within BT1108 at 1396.480 kb on - strand, within BT1108 at 1396.516 kb on - strand, within BT1108 at 1396.580 kb on - strand, within BT1108 at 1396.600 kb on + strand, within BT1108 at 1396.612 kb on + strand, within BT1108 at 1396.628 kb on + strand at 1396.629 kb on - strand at 1396.648 kb on + strand at 1396.718 kb on + strand at 1396.735 kb on + strand at 1396.764 kb on + strand at 1396.788 kb on + strand, within BT1109 at 1396.792 kb on + strand, within BT1109 at 1396.798 kb on + strand, within BT1109 at 1396.798 kb on + strand, within BT1109 at 1396.846 kb on + strand, within BT1109 at 1396.847 kb on - strand, within BT1109 at 1396.858 kb on + strand, within BT1109 at 1396.861 kb on + strand, within BT1109 at 1396.888 kb on + strand, within BT1109 at 1396.888 kb on + strand, within BT1109 at 1396.892 kb on + strand, within BT1109 at 1397.209 kb on - strand at 1397.286 kb on + strand at 1397.291 kb on - strand at 1397.298 kb on + strand at 1397.438 kb on + strand, within BT1110 at 1397.438 kb on + strand, within BT1110 at 1397.456 kb on + strand, within BT1110 at 1397.457 kb on - strand, within BT1110 at 1397.511 kb on + strand, within BT1110 at 1397.541 kb on + strand at 1397.546 kb on + strand at 1397.579 kb on + strand at 1397.587 kb on - strand at 1397.765 kb on - strand, within BT1111 at 1397.775 kb on - strand, within BT1111 at 1397.829 kb on - strand, within BT1111 at 1397.852 kb on - strand, within BT1111 at 1397.854 kb on - strand, within BT1111 at 1397.854 kb on - strand, within BT1111 at 1397.855 kb on + strand, within BT1111 at 1397.856 kb on - strand, within BT1111 at 1397.869 kb on - strand, within BT1111 at 1397.869 kb on - strand, within BT1111 at 1397.872 kb on + strand, within BT1111 at 1397.872 kb on + strand, within BT1111 at 1397.873 kb on - strand, within BT1111 at 1397.876 kb on + strand, within BT1111 at 1397.877 kb on - strand, within BT1111 at 1397.897 kb on + strand, within BT1111 at 1397.898 kb on - strand, within BT1111 at 1397.898 kb on - strand, within BT1111 at 1398.142 kb on + strand at 1398.145 kb on - strand at 1398.147 kb on - strand at 1398.147 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Trimethoprim 0.0125 mM remove 1,395,762 + BT1107 0.67 -1.2 1,395,762 + BT1107 0.67 -1.4 1,395,763 - BT1107 0.67 -2.4 1,395,851 - BT1107 0.85 +0.6 1,395,938 + -2.4 1,396,285 + BT1108 0.43 -2.4 1,396,353 + BT1108 0.52 +1.2 1,396,480 - BT1108 0.70 +1.4 1,396,516 - BT1108 0.75 +0.6 1,396,580 - BT1108 0.84 -0.4 1,396,600 + BT1108 0.86 -1.4 1,396,612 + BT1108 0.88 -2.1 1,396,628 + -1.4 1,396,629 - +0.6 1,396,648 + -0.4 1,396,718 + +0.1 1,396,735 + -0.6 1,396,764 + -2.0 1,396,788 + BT1109 0.14 -4.1 1,396,792 + BT1109 0.15 -2.4 1,396,798 + BT1109 0.17 +0.3 1,396,798 + BT1109 0.17 -2.7 1,396,846 + BT1109 0.27 +0.1 1,396,847 - BT1109 0.27 +0.6 1,396,858 + BT1109 0.30 -2.8 1,396,861 + BT1109 0.30 -2.4 1,396,888 + BT1109 0.36 +0.3 1,396,888 + BT1109 0.36 -0.4 1,396,892 + BT1109 0.37 -2.4 1,397,209 - -2.0 1,397,286 + -3.0 1,397,291 - +0.6 1,397,298 + -0.4 1,397,438 + BT1110 0.48 -1.8 1,397,438 + BT1110 0.48 -3.3 1,397,456 + BT1110 0.57 -2.3 1,397,457 - BT1110 0.57 -0.7 1,397,511 + BT1110 0.83 +1.1 1,397,541 + -1.0 1,397,546 + -2.4 1,397,579 + -0.6 1,397,587 - +0.4 1,397,765 - BT1111 0.18 -0.4 1,397,775 - BT1111 0.23 -2.8 1,397,829 - BT1111 0.47 +2.7 1,397,852 - BT1111 0.58 -0.4 1,397,854 - BT1111 0.59 -1.6 1,397,854 - BT1111 0.59 +2.7 1,397,855 + BT1111 0.59 +2.6 1,397,856 - BT1111 0.59 -2.7 1,397,869 - BT1111 0.65 +1.1 1,397,869 - BT1111 0.65 +1.1 1,397,872 + BT1111 0.67 -2.3 1,397,872 + BT1111 0.67 -2.0 1,397,873 - BT1111 0.67 -0.4 1,397,876 + BT1111 0.68 -0.4 1,397,877 - BT1111 0.69 -1.4 1,397,897 + BT1111 0.78 +2.7 1,397,898 - BT1111 0.78 +1.1 1,397,898 - BT1111 0.78 +3.3 1,398,142 + -3.6 1,398,145 - -0.5 1,398,147 - +2.4 1,398,147 - -1.4
Or see this region's nucleotide sequence